Strain identifier

BacDive ID: 12763

Type strain: No

Species: Pseudomonas aeruginosa

Strain Designation: PAO

Strain history: <- P.V. Phibbs <- B.W. Holloway, PAO

NCBI tax ID(s): 287 (species)

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General

@ref: 864

BacDive-ID: 12763

DSM-Number: 1707

keywords: Bacteria, mesophilic, human pathogen

description: Pseudomonas aeruginosa PAO is a mesophilic human pathogen of the family Pseudomonadaceae.

NCBI tax id

  • NCBI tax id: 287
  • Matching level: species

strain history

  • @ref: 864
  • history: <- P.V. Phibbs <- B.W. Holloway, PAO

doi: 10.13145/bacdive12763.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas aeruginosa
  • full scientific name: Pseudomonas aeruginosa (Schroeter 1872) Migula 1900 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Bacterium aeruginosum

@ref: 864

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas aeruginosa

full scientific name: Pseudomonas aeruginosa (Schroeter 1872) Migula 1900

strain designation: PAO

type strain: no

Morphology

multimedia

  • @ref: 864
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_1707.jpg
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

  • @ref: 864
  • name: NUTRIENT AGAR (DSMZ Medium 1)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1
  • composition: Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water

culture temp

  • @ref: 864
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

compound production

  • @ref: 864
  • compound: pyruvate carboxylase

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate+assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose+assimilation
683694853esculin-hydrolysis
6836916199urea+hydrolysis
6836929016arginine+hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369beta-glucosidase-3.2.1.21
68369urease+3.5.1.5
68369arginine dihydrolase+3.5.3.6

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43271C14:00.9
    43271C16:013.3
    43271C18:07.8
    43271C13:0 iso1.9
    43271C14:0 iso1
    43271C15:0 iso26.2
    43271C15:0 anteiso2.5
    43271C15:1ω6c0.6
    43271C16:1ω5c4.1
    43271C15:0 iso 3OH8
    43271C16:0 iso 3OH3.1
    43271C17:0 iso 3OH10.9
    43271C16:1ω7c / C16:1ω6c10.7
  • type of FA analysis: whole cell analysis
  • incubation medium: R2A
  • agar/liquid: agar
  • incubation temperature: 30
  • incubation time: 2
  • system: MIS MIDI

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
864+--++-+-+--++-+++++-+
864+--++-+/--+--++/--+++++-+

Safety information

risk assessment

  • @ref: 864
  • pathogenicity human: yes
  • pathogenicity animal: yes
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

External links

@ref: 864

culture collection no.: DSM 1707

straininfo link

  • @ref: 81972
  • straininfo: 47477

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology1459083A PacI/SwaI map of the Pseudomonas aeruginosa PAO chromosome.Romling U, Tummler BElectrophoresis10.1002/elps.115013011351992Base Sequence, DNA, Bacterial/genetics, Deoxyribonucleases, Type II Site-Specific, Electrophoresis, Gel, Pulsed-Field, Pseudomonas aeruginosa/*genetics, Restriction Mapping
Enzymology1905802The impact of two-dimensional pulsed-field gel electrophoresis techniques for the consistent and complete mapping of bacterial genomes: refined physical map of Pseudomonas aeruginosa PAO.Romling U, Tummler BNucleic Acids Res10.1093/nar/19.12.31991991Blotting, Southern, DNA Replication, DNA, Bacterial, Deoxyribonucleases, Type II Site-Specific/metabolism, Electrophoresis, Agar Gel/*methods, Electrophoresis, Gel, Two-Dimensional/*methods, Ethidium, Genes, Bacterial, Pseudomonas aeruginosa/*genetics, *Restriction MappingGenetics
Enzymology2512121A physical genome map of Pseudomonas aeruginosa PAO.Romling U, Grothues D, Bautsch W, Tummler BEMBO J10.1002/j.1460-2075.1989.tb08592.x1989Base Sequence, Blotting, Southern, *Chromosomes, Bacterial, DNA, Bacterial/genetics/isolation & purification, Electrophoresis, Gel, Two-Dimensional, *Genes, Bacterial, *Genomic Library, Molecular Sequence Data, Nucleic Acid Hybridization, Pseudomonas aeruginosa/*genetics, Restriction MappingPhylogeny
Enzymology7684678Comparative mapping of the Pseudomonas aeruginosa PAO genome with rare-cutter linking clones or two-dimensional pulsed-field gel electrophoresis protocols.Romling U, Tummler BElectrophoresis10.1002/elps.11501401501993DNA, Bacterial/analysis, Deoxyribonucleases, Type II Site-Specific, Electrophoresis, Gel, Pulsed-Field/*methods, Ethidium, Gene Library, *Genome, Bacterial, Nucleic Acid Hybridization, Pseudomonas aeruginosa/*genetics, *Restriction Mapping, Staining and LabelingGenetics

Reference

@idauthorscataloguedoi/urltitle
864Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 1707)https://www.dsmz.de/collection/catalogue/details/culture/DSM-1707
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
68369Automatically annotated from API 20NE
81972Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID47477.1StrainInfo: A central database for resolving microbial strain identifiers