Strain identifier
BacDive ID: 12762
Type strain: 
Species: Pseudomonas aeruginosa
Strain history: <- NCTC <- R. Sutherland
NCBI tax ID(s): 287 (species)
version 8.1 (current version)
General
@ref: 630
BacDive-ID: 12762
DSM-Number: 1299
keywords: Bacteria, mesophilic, human pathogen
description: Pseudomonas aeruginosa DSM 1299 is a mesophilic human pathogen that was isolated from sputum.
NCBI tax id
- NCBI tax id: 287
- Matching level: species
strain history
- @ref: 630
- history: <- NCTC <- R. Sutherland
doi: 10.13145/bacdive12762.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Pseudomonadales
- family: Pseudomonadaceae
- genus: Pseudomonas
- species: Pseudomonas aeruginosa
- full scientific name: Pseudomonas aeruginosa (Schroeter 1872) Migula 1900 (Approved Lists 1980)
synonyms
- @ref: 20215
- synonym: Bacterium aeruginosum
@ref: 630
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Pseudomonadales
family: Pseudomonadaceae
genus: Pseudomonas
species: Pseudomonas aeruginosa
full scientific name: Pseudomonas aeruginosa (Schroeter 1872) Migula 1900
type strain: no
Culture and growth conditions
culture medium
- @ref: 630
- name: NUTRIENT AGAR (DSMZ Medium 1)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1
- composition: Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
culture temp
- @ref: 630
- growth: positive
- type: growth
- temperature: 37
- range: mesophilic
Physiology and metabolism
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 68369 | 25115 | malate | + | assimilation |
| 68369 | 17128 | adipate | + | assimilation |
| 68369 | 27689 | decanoate | + | assimilation |
| 68369 | 24265 | gluconate | - | assimilation |
| 68369 | 17306 | maltose | - | assimilation |
| 68369 | 59640 | N-acetylglucosamine | + | assimilation |
| 68369 | 16899 | D-mannitol | - | assimilation |
| 68369 | 16024 | D-mannose | - | assimilation |
| 68369 | 30849 | L-arabinose | - | assimilation |
| 68369 | 17634 | D-glucose | + | assimilation |
| 68369 | 5291 | gelatin | - | hydrolysis |
| 68369 | 4853 | esculin | - | hydrolysis |
| 68369 | 16199 | urea | + | hydrolysis |
| 68369 | 29016 | arginine | + | hydrolysis |
| 68369 | 17634 | D-glucose | - | fermentation |
| 68369 | 27897 | tryptophan | - | energy source |
| 68369 | 17632 | nitrate | + | reduction |
metabolite production
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 68369 | cytochrome oxidase | + | 1.9.3.1 |
| 68369 | gelatinase | - | |
| 68369 | beta-glucosidase | - | 3.2.1.21 |
| 68369 | urease | + | 3.5.1.5 |
| 68369 | arginine dihydrolase | + | 3.5.3.6 |
API 20NE
| @ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC | OX |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 630 | + | - | - | + | + | - | - | - | + | - | - | - | + | - | - | + | + | + | + | - | + |
Isolation, sampling and environmental information
isolation
- @ref: 630
- sample type: sputum
isolation source categories
- Cat1: #Host Body Product
- Cat2: #Fluids
- Cat3: #Sputum
Safety information
risk assessment
- @ref: 630
- pathogenicity human: yes
- pathogenicity animal: yes
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
External links
@ref: 630
culture collection no.: DSM 1299, ATCC 23389, NCTC 10701
straininfo link
- @ref: 81971
- straininfo: 47348
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Enzymology | 96731 | Beta-lactamases: determination of their isoelectric points. | Barthelemy M, Guionie M, Labia R | Antimicrob Agents Chemother | 10.1128/AAC.13.4.695 | 1978 | Amidohydrolases/*analysis, Cephalosporinase/*analysis, Escherichia coli/enzymology, Isoelectric Focusing/methods, Isoelectric Point, Molecular Weight, Pseudomonas aeruginosa/enzymology | |
| Pathogenicity | 3997707 | Human pharmacokinetics of temocillin (BRL 17421) side chain epimers. | Guest EA, Horton R, Mellows G, Slocombe B, Swaisland AJ, Tasker TC | J Antimicrob Chemother | 10.1093/jac/15.3.327 | 1985 | Adult, Humans, Isomerism, Male, Metabolic Clearance Rate, Penicillins/*metabolism, Structure-Activity Relationship | Enzymology |
| Metabolism | 4029021 | Human pharmacokinetics and antimicrobial activities of the temocillin epimers. | Guest EA, Horton R, Mellows G, Slocombe B, Swaisland AJ, Tasker TC, White AR | Drugs | 10.2165/00003495-198500295-00032 | 1985 | Adult, Blood Proteins/metabolism, Humans, Isomerism, Male, Metabolic Clearance Rate, Microbial Sensitivity Tests, Penicillins/*metabolism/pharmacology, Protein Binding, Structure-Activity Relationship | Pathogenicity |
| Pathogenicity | 23303259 | [Discrepancy in the disk diffusion susceptibility test of Pseudomonas aeruginosa strains isolated from cystic fibrosis patients after anaerobic preincubation and its potential clinical relevance]. | Cafini F, Garcia-Rey C, Bas P, Gomez-Lus ML, Sanchez I, Vazquez S, Prieto J | Rev Esp Quimioter | cafini | 2012 | Aerobiosis, Anaerobiosis, Anti-Bacterial Agents/pharmacology, Cystic Fibrosis/*complications, *Disk Diffusion Antimicrobial Tests, Drug Resistance, Bacterial/*physiology, Expectorants/pharmacology, Humans, Microbial Viability, Mucus/microbiology, Oxygen/metabolism, Pseudomonas Infections/complications/*microbiology, Pseudomonas aeruginosa/*drug effects/growth & development/isolation & purification/metabolism, Respiratory System/microbiology | Metabolism |
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 630 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 1299) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-1299 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 68369 | Automatically annotated from API 20NE | |||
| 81971 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID47348.1 | StrainInfo: A central database for resolving microbial strain identifiers |