Strain identifier
BacDive ID: 12755
Type strain: 
Species: Azotobacter nigricans
Strain Designation: WR-128
Strain history: <- WR <- E. Afrikian <- N. A. Krassilnikov
NCBI tax ID(s): 270344 (species)
version 8.1 (current version)
General
@ref: 1020
BacDive-ID: 12755
DSM-Number: 2288
keywords: 16S sequence, Bacteria, mesophilic
description: Azotobacter nigricans WR-128 is a mesophilic bacterium of the family Pseudomonadaceae.
NCBI tax id
- NCBI tax id: 270344
- Matching level: species
strain history
- @ref: 1020
- history: <- WR <- E. Afrikian <- N. A. Krassilnikov
doi: 10.13145/bacdive12755.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Pseudomonadales
- family: Pseudomonadaceae
- genus: Azotobacter
- species: Azotobacter nigricans
- full scientific name: Azotobacter nigricans Krassilnikov 1949 (Approved Lists 1980)
@ref: 1020
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Pseudomonadales
family: Pseudomonadaceae
genus: Azotobacter
species: Azotobacter nigricans
full scientific name: Azotobacter nigricans Krassilnikov 1949
strain designation: WR-128
type strain: yes
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 1020 | DIAZOTROPHIC MEDIUM (RBA) (DSMZ Medium 441) | yes | https://mediadive.dsmz.de/medium/441 | Name: DIAZOTROPHIC MEDIUM (RBA) (DSMZ Medium 441) Composition: Agar 14.881 g/l DL-Malate 1.98413 g/l D-Glucose 1.98413 g/l D-Mannitol 1.98413 g/l Na-pyruvate 0.992063 g/l Disodium succinate 0.992063 g/l K2HPO4 0.892857 g/l KH2PO4 0.0992065 g/l NaCl 0.0992065 g/l CaCl2 x 2 H2O 0.0992065 g/l MgSO4 x 7 H2O 0.0992065 g/l Yeast extract 0.0496032 g/l FeSO4 x 7 H2O 0.00992065 g/l MnSO4 x H2O 0.00496032 g/l NaVO3 x H2O 0.00496032 g/l Nicotinic acid 0.00248016 g/l Pyridoxine hydrochloride 0.00248016 g/l Calcium pantothenate 0.00248016 g/l Thiamine-HCl x 2 H2O 0.00248016 g/l H3BO3 0.000892857 g/l CoCl2 x 6 H2O 0.000595238 g/l Riboflavin 0.000496032 g/l ZnSO4 x 7 H2O 0.000297619 g/l MnCl2 x 4 H2O 8.92857e-05 g/l Na2MoO4 x 2 H2O 8.92857e-05 g/l NiCl2 x 6 H2O 5.95238e-05 g/l Vitamin B12 4.96032e-05 g/l CuCl2 x 2 H2O 2.97619e-05 g/l Folic acid 9.92063e-06 g/l Biotin 4.96032e-06 g/l Distilled water |
| 1020 | AZOTOBACTER MEDIUM (DSMZ Medium 3) | yes | https://mediadive.dsmz.de/medium/3 | Name: AZOTOBACTER MEDIUM (DSMZ Medium 3) Composition: Agar 15.0 g/l CaCO3 5.0 g/l Glucose 5.0 g/l Mannitol 5.0 g/l K2HPO4 0.9 g/l KH2PO4 0.1 g/l MgSO4 x 7 H2O 0.1 g/l CaCl2 x 2 H2O 0.1 g/l FeSO4 x 7 H2O 0.01 g/l Na2MoO4 x 2 H2O 0.005 g/l Distilled water |
culture temp
- @ref: 1020
- growth: positive
- type: growth
- temperature: 30
- range: mesophilic
Safety information
risk assessment
- @ref: 1020
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 1020
- description: Azotobacter nigricans subsp. nigricans gene for 16S rRNA, partial sequence
- accession: AB175651
- length: 1475
- database: ena
- NCBI tax ID: 270492
GC content
- @ref: 1020
- GC-content: 64.5
External links
@ref: 1020
culture collection no.: DSM 2288, ATCC 35009, UQM 1967
straininfo link
- @ref: 81964
- straininfo: 1079
literature
- topic: Enzymology
- Pubmed-ID: 1987127
- title: Detection of alternative nitrogenases in aerobic gram-negative nitrogen-fixing bacteria.
- authors: Fallik E, Chan YK, Robson RL
- journal: J Bacteriol
- DOI: 10.1128/jb.173.1.365-371.1991
- year: 1991
- mesh: Azotobacter/enzymology/genetics, DNA Probes, DNA, Bacterial/genetics/isolation & purification, Escherichia coli/genetics, *Genes, Bacterial, Gram-Negative Aerobic Bacteria/enzymology/*genetics, *Nitrogen Fixation, Nitrogenase/*genetics, Nucleic Acid Hybridization, Pseudomonas/enzymology/genetics, Rhizobium/enzymology/genetics, Species Specificity
- topic2: Phylogeny
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 1020 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2288) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-2288 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 81964 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID1079.1 | StrainInfo: A central database for resolving microbial strain identifiers |