Strain identifier

BacDive ID: 1275

Type strain: Yes

Species: Bacillus decisifrondis

Strain Designation: E5HC-32

Strain history: <- L. Zhang; E5HC-32

NCBI tax ID(s): 380630 (species)

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General

@ref: 6921

BacDive-ID: 1275

DSM-Number: 17725

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped

description: Bacillus decisifrondis E5HC-32 is an aerobe, spore-forming, mesophilic bacterium that was isolated from forest soil.

NCBI tax id

  • NCBI tax id: 380630
  • Matching level: species

strain history

  • @ref: 6921
  • history: <- L. Zhang; E5HC-32

doi: 10.13145/bacdive1275.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Bacillus
  • species: Bacillus decisifrondis
  • full scientific name: Bacillus decisifrondis Zhang et al. 2007

@ref: 6921

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Bacillus

species: Bacillus decisifrondis

full scientific name: Bacillus decisifrondis Zhang et al. 2007

strain designation: E5HC-32

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
31826positive3.7 µm1.2 µmrod-shapedyes
69480yes97.672
69480positive100

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6921CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
6921NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
6921positivegrowth30mesophilic
31826positivegrowth25-40
31826positiveoptimum40thermophilic

culture pH

@refabilitytypepHPH range
31826positivegrowth7.1-9.1alkaliphile
31826positiveoptimum8.4

Physiology and metabolism

oxygen tolerance

  • @ref: 31826
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
31826yes
69481yes100
69480yes100

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3182621217L-alaninamide+carbon source
3182616449alanine+carbon source
3182640585alpha-cyclodextrin+carbon source
31826286442-oxopentanoate+carbon source
3182624148galactonate+carbon source
3182617754glycerol+carbon source
3182617596inosine+carbon source
3182617716lactose+carbon source
3182626271proline+carbon source
3182615361pyruvate+carbon source
3182616634raffinose+carbon source
3182626546rhamnose+carbon source
3182630911sorbitol+carbon source
3182617748thymidine+carbon source
3182616704uridine+carbon source
3182627248urocanic acid+carbon source

enzymes

  • @ref: 31826
  • value: catalase
  • activity: +
  • ec: 1.11.1.6

Isolation, sampling and environmental information

isolation

  • @ref: 6921
  • sample type: forest soil
  • geographic location: Southeast Queensland
  • country: Australia
  • origin.country: AUS
  • continent: Australia and Oceania

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Forest
#Environmental#Terrestrial#Soil

Safety information

risk assessment

  • @ref: 6921
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 6921
  • description: UNVERIFIED: Bacillus decisifrondis strain E5HC-32 16S ribosomal RNA gene, partial sequence
  • accession: DQ465405
  • length: 1305
  • database: ena
  • NCBI tax ID: 380630

Genome sequences

  • @ref: 66792
  • description: Bacillus decisifrondis strain DSM 17725
  • accession: 380630.3
  • assembly level: wgs
  • database: patric
  • NCBI tax ID: 380630

GC content

  • @ref: 6921
  • GC-content: 41

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
gram-positiveyes89.327no
anaerobicno96.432yes
halophileno91.084no
spore-formingyes97.39yes
glucose-utilyes83.311no
aerobicyes88.217no
motileyes88.522no
flagellatedyes84.42no
thermophileno99.764no
glucose-fermentno88.787no

External links

@ref: 6921

culture collection no.: DSM 17725, JCM 13601

straininfo link

  • @ref: 70929
  • straininfo: 404034

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny17473244Bacillus decisifrondis sp. nov., isolated from soil underlying decaying leaf foliage.Zhang L, Xu Z, Patel BKCInt J Syst Evol Microbiol10.1099/ijs.0.64440-02007Amino Acids/metabolism, Australia, Bacillus/*classification/genetics/*isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, Carbohydrate Metabolism, Cell Wall/ultrastructure, Culture Media, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Flagella/ultrastructure, Genes, rRNA/genetics, Hydrogen-Ion Concentration, Microscopy, Electron, Transmission, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, *Soil Microbiology, Spores, Bacterial/cytology, Temperature, Thymidine/metabolism, Trees, Uridine/metabolismMetabolism
Genetics26494684Genome Sequence of Type Strain Bacillus decisifrondis E5HC-32T (DSM 11725T), Isolated from Soil Underlying the Decaying Leaf Litter of a Slash Pine Forest.Liu GH, Liu B, Wang JP, Chen QQ, Che JM, Zheng XF, Ge CBGenome Announc10.1128/genomeA.01249-152015Phylogeny

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
6921Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17725)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17725
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
31826Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2809228776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
70929Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID404034.1StrainInfo: A central database for resolving microbial strain identifiers