Workshop and Q&A October 6th

Strain identifier

BacDive ID: 12736

Type strain: Yes

Species: Azotobacter chroococcum

Strain Designation: 43

Strain history: IAM 12666 <-- ATCC 9043 <-- R. L. Starkey 43.

NCBI tax ID(s): 353 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 1019

BacDive-ID: 12736

DSM-Number: 2286

keywords: Bacteria, 16S sequence, genome sequence, mesophilic

description: Azotobacter chroococcum 43 is a mesophilic bacterium of the family Pseudomonadaceae.

NCBI tax id

  • NCBI tax id: 353
  • Matching level: species

strain history

doi: 10.13145/bacdive12736.20220920.7

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Azotobacter
  • species: Azotobacter chroococcum
  • full scientific name: Azotobacter chroococcum Beijerinck 1901 (Approved Lists 1980)

@ref: 1019

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Azotobacter

species: Azotobacter chroococcum

full scientific name: Azotobacter chroococcum Beijerinck 1901

strain designation: 43

type strain: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
1019DIAZOTROPHIC MEDIUM (RBA) (DSMZ Medium 441)yeshttps://bacmedia.dsmz.de/medium/441Name: DIAZOTROPHIC MEDIUM (RBA) (DSMZ Medium 441) Composition: Agar 14.881 g/l DL-Malate 1.98413 g/l D-Glucose 1.98413 g/l D-Mannitol 1.98413 g/l Na-pyruvate 0.992063 g/l Disodium succinate 0.992063 g/l K2HPO4 0.892857 g/l KH2PO4 0.0992065 g/l NaCl 0.0992065 g/l CaCl2 x 2 H2O 0.0992065 g/l MgSO4 x 7 H2O 0.0992065 g/l Yeast extract 0.0496032 g/l FeSO4 x 7 H2O 0.00992065 g/l MnSO4 x H2O 0.00496032 g/l NaVO3 x H2O 0.00496032 g/l Nicotinic acid 0.00248016 g/l Thiamine-HCl x 2 H2O 0.00248016 g/l Calcium pantothenate 0.00248016 g/l Pyridoxine hydrochloride 0.00248016 g/l H3BO3 0.000892857 g/l CoCl2 x 6 H2O 0.000595238 g/l Riboflavin 0.000496032 g/l ZnSO4 x 7 H2O 0.000297619 g/l MnCl2 x 4 H2O 8.92857e-05 g/l Na2MoO4 x 2 H2O 8.92857e-05 g/l NiCl2 x 6 H2O 5.95238e-05 g/l Vitamin B12 4.96032e-05 g/l CuCl2 x 2 H2O 2.97619e-05 g/l Folic acid 9.92063e-06 g/l Biotin 4.96032e-06 g/l Distilled water
1019AZOTOBACTER MEDIUM (DSMZ Medium 3)yeshttps://bacmedia.dsmz.de/medium/3Name: AZOTOBACTER MEDIUM (DSMZ Medium 3) Composition: Agar 15.0 g/l CaCO3 5.0 g/l Glucose 5.0 g/l Mannitol 5.0 g/l K2HPO4 0.9 g/l KH2PO4 0.1 g/l MgSO4 x 7 H2O 0.1 g/l CaCl2 x 2 H2O 0.1 g/l FeSO4 x 7 H2O 0.01 g/l Na2MoO4 x 2 H2O 0.005 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
1019positivegrowth30mesophilic
67770positivegrowth25mesophilic

Physiology and metabolism

compound production

  • @ref: 1019
  • compound: L carnitine

observation

  • @ref: 67770
  • observation: quinones: Q-9

Safety information

risk assessment

  • @ref: 1019
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Azotobacter chroococcum small subunit ribosomal RNA gene, partial sequenceAF035211959ena353
20218Azotobacter chroococcum strain ICMP 15214 16S ribosomal RNA gene, partial sequenceEF1001541371ena353
20218Azotobacter chroococcum 16S ribosomal RNA gene, partial sequenceAY3537081383ena353
20218Azotobacter chroococcum gene for 16S rRNA, partial sequenceAB1756531430ena353
20218Azotobacter chroococcum strain LMG 8756 16S ribosomal RNA gene, partial sequenceEU4019061501ena353
20218Azotobacter chroococcum gene for 16S rRNA, partial sequence, strain: NBRC 102613AB6818871461ena353

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Azotobacter chroococcum ATCC 9043GCA_004327905scaffoldncbi353
66792Azotobacter chroococcum DSM 2286GCA_004339665scaffoldncbi353
66792Azotobacter chroococcum strain ATCC 9043353.12wgspatric353
66792Azotobacter chroococcum strain DSM 2286353.14wgspatric353
66792Azotobacter chroococcum DSM 22862802428852draftimg353
67770Azotobacter chroococcum strain ATCC 9043, whole genome shotgun sequencing projectSJAA00000000ncbi353
67770Azotobacter chroococcum strain DSM 2286, whole genome shotgun sequencing projectSMMU00000000ncbi353

External links

@ref: 1019

culture collection no.: DSM 2286, ATCC 9043, VKM B-1616, CCUG 53675, LMG 8756, CECT 4103, IAM 12666, IAM 15040, JCM 20725, BCRC 10599, ICMP 15214, JCM 21503, NBRC 102613, NCAIM B.01391, NCIMB 11694, NRRL B-14346, NRRL B-14637

straininfo link

@refpassport
20218http://www.straininfo.net/strains/4349
20218http://www.straininfo.net/strains/4348
20218http://www.straininfo.net/strains/157849

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology1987127Detection of alternative nitrogenases in aerobic gram-negative nitrogen-fixing bacteria.Fallik E, Chan YK, Robson RLJ Bacteriol10.1128/jb.173.1.365-371.19911991Azotobacter/enzymology/genetics, DNA Probes, DNA, Bacterial/genetics/isolation & purification, Escherichia coli/genetics, *Genes, Bacterial, Gram-Negative Aerobic Bacteria/enzymology/*genetics, *Nitrogen Fixation, Nitrogenase/*genetics, Nucleic Acid Hybridization, Pseudomonas/enzymology/genetics, Rhizobium/enzymology/genetics, Species SpecificityPhylogeny
Metabolism8415713Effects of Mn2+ and Mg2+ on assimilation of NO3- and NH4+ by soil microorganisms.McCarty GW, Bremner JMProc Natl Acad Sci U S A10.1073/pnas.90.20.94031993Azotobacter/*metabolism, Magnesium/*pharmacology, Manganese/*pharmacology, Nitrates/*metabolism, Pseudomonas/*metabolism, Quaternary Ammonium Compounds/*metabolism, Soil Microbiology
Phylogeny31046894Azotobacter bryophylli sp. nov., isolated from the succulent plant Bryophyllum pinnatum.Liu L, Yuan T, An Q, Yang M, Mao X, Mo C, Tan Z, Peng GInt J Syst Evol Microbiol10.1099/ijsem.0.0034122019Azotobacter/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA Fingerprinting, DNA, Bacterial/genetics, Fatty Acids/chemistry, Kalanchoe/*microbiology, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Plant Leaves/microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Ubiquinone/chemistryTranscriptome
Phylogeny31995466Description of Azotobacter chroococcum subsp. isscasi subsp. nov. isolated from paddy soil and establishment of Azotobacter chroococcum subsp. chroococcum subsp. nov.Jin H, Wang H, Zhang Y, Hu T, Lin Z, Liu B, Ma J, Wang X, Liu Q, Lin X, Xie ZInt J Syst Evol Microbiol10.1099/ijsem.0.0040262020Azotobacter/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, Base Sequence, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Oryza, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil MicrobiologyTranscriptome
Metabolism33436213Characterization of a novel endo-levanase from Azotobacter chroococcum DSM 2286 and its application for the production of prebiotic fructooligosaccharides.Hovels M, Kosciow K, Deppenmeier UCarbohydr Polym10.1016/j.carbpol.2020.1173842020Azotobacter/*enzymology/genetics, Bacterial Proteins/genetics/*metabolism, Disaccharides/chemistry/metabolism, Escherichia coli/enzymology/genetics, Fructans/chemistry/metabolism, Fructose/chemistry/metabolism, Gene Expression, Gluconobacter/*enzymology/genetics, Glycoside Hydrolases/genetics/*metabolism, Hexosyltransferases/genetics/*metabolism, Humans, Hydrolysis, Oligosaccharides/*biosynthesis/chemistry, Phleum/chemistry, Prebiotics/*analysis, Recombinant Proteins/genetics/metabolism, Sucrose/chemistry/metabolismEnzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
1019Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2286)https://www.dsmz.de/collection/catalogue/details/culture/DSM-2286
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/