Archive BacDiveID:10.13145/bacdive12736.20230509.8.1

Strain identifier

BacDive ID: 12736

Type strain:

Species: Azotobacter chroococcum

Strain Designation: 43

Strain history: IAM 12666 <-- ATCC 9043 <-- R. L. Starkey 43.

NCBI tax ID(s): 353 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 1019

BacDive-ID: 12736

DSM-Number: 2286

keywords: genome sequence, 16S sequence, Bacteria, mesophilic

description: Azotobacter chroococcum 43 is a mesophilic bacterium of the family Pseudomonadaceae.

NCBI tax id

NCBI tax id: 353
Matching level: species

strain history

@ref	history
1019	<- ATCC <- R.L. Starkey, 43
67770	IAM 12666 <-- ATCC 9043 <-- R. L. Starkey 43.

doi: 10.13145/bacdive12736.20230509.8.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/bacteria
keyword: phylum/pseudomonadota
domain: Bacteria
phylum: Pseudomonadota
class: Gammaproteobacteria
order: Pseudomonadales
family: Pseudomonadaceae
genus: Azotobacter
species: Azotobacter chroococcum
full scientific name: Azotobacter chroococcum Beijerinck 1901 (Approved Lists 1980)

@ref: 1019

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Azotobacter

species: Azotobacter chroococcum

full scientific name: Azotobacter chroococcum Beijerinck 1901

strain designation: 43

type strain: yes

Culture and growth conditions

culture medium

@ref: 1019
name: DIAZOTROPHIC MEDIUM (RBA) (DSMZ Medium 441)
growth: yes
link: https://mediadive.dsmz.de/medium/441
composition: Name: DIAZOTROPHIC MEDIUM (RBA) (DSMZ Medium 441) Composition: Agar 14.881 g/l DL-Malate 1.98413 g/l D-Glucose 1.98413 g/l D-Mannitol 1.98413 g/l Na-pyruvate 0.992063 g/l Disodium succinate 0.992063 g/l K2HPO4 0.892857 g/l KH2PO4 0.0992065 g/l NaCl 0.0992065 g/l CaCl2 x 2 H2O 0.0992065 g/l MgSO4 x 7 H2O 0.0992065 g/l Yeast extract 0.0496032 g/l FeSO4 x 7 H2O 0.00992065 g/l MnSO4 x H2O 0.00496032 g/l NaVO3 x H2O 0.00496032 g/l Nicotinic acid 0.00248016 g/l Pyridoxine hydrochloride 0.00248016 g/l Calcium pantothenate 0.00248016 g/l Thiamine-HCl x 2 H2O 0.00248016 g/l H3BO3 0.000892857 g/l CoCl2 x 6 H2O 0.000595238 g/l Riboflavin 0.000496032 g/l ZnSO4 x 7 H2O 0.000297619 g/l MnCl2 x 4 H2O 8.92857e-05 g/l Na2MoO4 x 2 H2O 8.92857e-05 g/l NiCl2 x 6 H2O 5.95238e-05 g/l Vitamin B12 4.96032e-05 g/l CuCl2 x 2 H2O 2.97619e-05 g/l Folic acid 9.92063e-06 g/l Biotin 4.96032e-06 g/l Distilled water

culture temp

@ref	growth	type	temperature	range
1019	positive	growth	30	mesophilic
67770	positive	growth	25	mesophilic

Physiology and metabolism

compound production

@ref: 1019
compound: L carnitine

observation

@ref: 67770
observation: quinones: Q-9

Safety information

risk assessment

@ref: 1019
biosafety level: 1
biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@ref	description	accession	length	database	NCBI tax ID
20218	Azotobacter chroococcum small subunit ribosomal RNA gene, partial sequence	AF035211	959	ena	353
20218	Azotobacter chroococcum strain ICMP 15214 16S ribosomal RNA gene, partial sequence	EF100154	1371	ena	353
20218	Azotobacter chroococcum 16S ribosomal RNA gene, partial sequence	AY353708	1383	ena	353
20218	Azotobacter chroococcum gene for 16S rRNA, partial sequence	AB175653	1430	ena	353
20218	Azotobacter chroococcum strain LMG 8756 16S ribosomal RNA gene, partial sequence	EU401906	1501	ena	353
20218	Azotobacter chroococcum gene for 16S rRNA, partial sequence, strain: NBRC 102613	AB681887	1461	ena	353

Genome sequences

@ref	description	accession	assembly level	database	NCBI tax ID
66792	Azotobacter chroococcum strain ATCC 9043	353.12	wgs	patric	353
66792	Azotobacter chroococcum strain DSM 2286	353.14	wgs	patric	353
66792	Azotobacter chroococcum DSM 2286	2802428852	draft	img	353
67770	Azotobacter chroococcum ATCC 9043	GCA_004327905	scaffold	ncbi	353
67770	Azotobacter chroococcum DSM 2286	GCA_004339665	scaffold	ncbi	353

Genome-based predictions

predictions

trait	prediction	confidence	training_data
gram-positive	no	98.794	no
anaerobic	no	96.786	no
halophile	no	89.939	no
spore-forming	no	94.178	no
glucose-util	yes	83.918	no
motile	yes	88.458	no
flagellated	yes	79.148	no
aerobic	yes	85.589	no
thermophile	no	98.41	yes
glucose-ferment	no	89.165	no

External links

@ref: 1019

culture collection no.: DSM 2286, ATCC 9043, VKM B-1616, CCUG 53675, LMG 8756, CECT 4103, IAM 12666, IAM 15040, JCM 20725, BCRC 10599, ICMP 15214, JCM 21503, NBRC 102613, NCAIM B.01391, NCIMB 11694, NRRL B-14346, NRRL B-14637

straininfo link

@ref: 81945
straininfo: 1078

literature

topic	Pubmed-ID	title	authors	journal	DOI	year	mesh	topic2
Enzymology	1987127	Detection of alternative nitrogenases in aerobic gram-negative nitrogen-fixing bacteria.	Fallik E, Chan YK, Robson RL	J Bacteriol	10.1128/jb.173.1.365-371.1991	1991	Azotobacter/enzymology/genetics, DNA Probes, DNA, Bacterial/genetics/isolation & purification, Escherichia coli/genetics, Genes, Bacterial, Gram-Negative Aerobic Bacteria/enzymology/genetics, Nitrogen Fixation, Nitrogenase/genetics, Nucleic Acid Hybridization, Pseudomonas/enzymology/genetics, Rhizobium/enzymology/genetics, Species Specificity	Phylogeny
Metabolism	8415713	Effects of Mn2+ and Mg2+ on assimilation of NO3- and NH4+ by soil microorganisms.	McCarty GW, Bremner JM	Proc Natl Acad Sci U S A	10.1073/pnas.90.20.9403	1993	Azotobacter/metabolism, Magnesium/pharmacology, Manganese/pharmacology, Nitrates/metabolism, Pseudomonas/metabolism, Quaternary Ammonium Compounds/metabolism, Soil Microbiology
Phylogeny	31046894	Azotobacter bryophylli sp. nov., isolated from the succulent plant Bryophyllum pinnatum.	Liu L, Yuan T, An Q, Yang M, Mao X, Mo C, Tan Z, Peng G	Int J Syst Evol Microbiol	10.1099/ijsem.0.003412	2019	Azotobacter/classification/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA Fingerprinting, DNA, Bacterial/genetics, Fatty Acids/chemistry, Kalanchoe/microbiology, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Plant Leaves/microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Ubiquinone/chemistry	Transcriptome
Phylogeny	31995466	Description of Azotobacter chroococcum subsp. isscasi subsp. nov. isolated from paddy soil and establishment of Azotobacter chroococcum subsp. chroococcum subsp. nov.	Jin H, Wang H, Zhang Y, Hu T, Lin Z, Liu B, Ma J, Wang X, Liu Q, Lin X, Xie Z	Int J Syst Evol Microbiol	10.1099/ijsem.0.004026	2020	Azotobacter/classification/isolation & purification, Bacterial Typing Techniques, Base Composition, Base Sequence, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Oryza, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology	Transcriptome
Metabolism	33436213	Characterization of a novel endo-levanase from Azotobacter chroococcum DSM 2286 and its application for the production of prebiotic fructooligosaccharides.	Hovels M, Kosciow K, Deppenmeier U	Carbohydr Polym	10.1016/j.carbpol.2020.117384	2020	Azotobacter/enzymology/genetics, Bacterial Proteins/genetics/metabolism, Disaccharides/chemistry/metabolism, Escherichia coli/enzymology/genetics, Fructans/chemistry/metabolism, Fructose/chemistry/metabolism, Gene Expression, Gluconobacter/enzymology/genetics, Glycoside Hydrolases/genetics/metabolism, Hexosyltransferases/genetics/metabolism, Humans, Hydrolysis, Oligosaccharides/biosynthesis/chemistry, Phleum/chemistry, Prebiotics/*analysis, Recombinant Proteins/genetics/metabolism, Sucrose/chemistry/metabolism	Enzymology

Reference

@id	authors	catalogue	doi/url	title	journal	pubmed
1019	Curators of the DSMZ	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2286)	https://www.dsmz.de/collection/catalogue/details/culture/DSM-2286
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.		10.1099/ijsem.0.004332	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.		10.1016/j.syapm.2013.11.002	StrainInfo introduces electronic passports for microorganisms.	Syst Appl Microbiol. 37: 42-50 2014	24321274
66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770	Curators of the JCM		https://jcm.brc.riken.jp/en/
81945	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.		10.60712/SI-ID1078.1	StrainInfo: A central database for resolving microbial strain identifiers