Archive BacDiveID:10.13145/bacdive12736.20250331.9.3

Strain identifier

BacDive ID: 12736

Type strain:

Species: Azotobacter chroococcum

Strain Designation: 43

Strain history: IAM 12666 <-- ATCC 9043 <-- R. L. Starkey 43.

NCBI tax ID(s): 353 (species), 2707774 (subspecies)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 1019

BacDive-ID: 12736

DSM-Number: 2286

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-negative

description: Azotobacter chroococcum 43 is a mesophilic, Gram-negative bacterium of the family Pseudomonadaceae.

NCBI tax id

NCBI tax id	Matching level
2707774	subspecies
353	species

strain history

@ref	history
1019	<- ATCC <- R.L. Starkey, 43
67770	IAM 12666 <-- ATCC 9043 <-- R. L. Starkey 43.

doi: 10.13145/bacdive12736.20250331.9.3

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/bacteria
keyword: phylum/pseudomonadota
domain: Bacteria
phylum: Pseudomonadota
class: Gammaproteobacteria
order: Pseudomonadales
family: Pseudomonadaceae
genus: Azotobacter
species: Azotobacter chroococcum
full scientific name: Azotobacter chroococcum Beijerinck 1901 (Approved Lists 1980)

@ref: 1019

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Azotobacter

species: Azotobacter chroococcum

full scientific name: Azotobacter chroococcum Beijerinck 1901

strain designation: 43

type strain: yes

Morphology

cell morphology

@ref	gram stain	confidence
125439	negative	94.4
125438	negative	98.5

Culture and growth conditions

culture medium

@ref	name	growth	link	composition
1019	DIAZOTROPHIC MEDIUM (RBA) (DSMZ Medium 441)	yes	https://mediadive.dsmz.de/medium/441	Name: DIAZOTROPHIC MEDIUM (RBA) (DSMZ Medium 441) Composition: Agar 14.881 g/l DL-Malate 1.98413 g/l D-Glucose 1.98413 g/l D-Mannitol 1.98413 g/l Na-pyruvate 0.992063 g/l Disodium succinate 0.992063 g/l K2HPO4 0.892857 g/l KH2PO4 0.0992065 g/l NaCl 0.0992065 g/l CaCl2 x 2 H2O 0.0992065 g/l MgSO4 x 7 H2O 0.0992065 g/l Yeast extract 0.0496032 g/l FeSO4 x 7 H2O 0.00992065 g/l MnSO4 x H2O 0.00496032 g/l NaVO3 x H2O 0.00496032 g/l Nicotinic acid 0.00248016 g/l Pyridoxine hydrochloride 0.00248016 g/l Calcium pantothenate 0.00248016 g/l Thiamine-HCl x 2 H2O 0.00248016 g/l H3BO3 0.000892857 g/l CoCl2 x 6 H2O 0.000595238 g/l Riboflavin 0.000496032 g/l ZnSO4 x 7 H2O 0.000297619 g/l MnCl2 x 4 H2O 8.92857e-05 g/l Na2MoO4 x 2 H2O 8.92857e-05 g/l NiCl2 x 6 H2O 5.95238e-05 g/l Vitamin B12 4.96032e-05 g/l CuCl2 x 2 H2O 2.97619e-05 g/l Folic acid 9.92063e-06 g/l Biotin 4.96032e-06 g/l Distilled water
1019	R2A MEDIUM (DSMZ Medium 830)	yes	https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium830.pdf

culture temp

@ref	growth	type	temperature
1019	positive	growth	30
67770	positive	growth	25
1019	positive	growth	28

Physiology and metabolism

spore formation

@ref: 125439
spore formation: no
confidence: 99

compound production

@ref: 1019
compound: L carnitine

observation

@ref: 67770
observation: quinones: Q-9

Safety information

risk assessment

@ref: 1019
biosafety level: 1
biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@ref	description	accession	length	database	NCBI tax ID
20218	Azotobacter chroococcum small subunit ribosomal RNA gene, partial sequence	AF035211	959	nuccore	353
20218	Azotobacter chroococcum strain ICMP 15214 16S ribosomal RNA gene, partial sequence	EF100154	1371	nuccore	353
20218	UNVERIFIED_ORG: Azotobacter chroococcum 16S ribosomal RNA gene, partial sequence	AY353708	1383	nuccore	353
20218	Azotobacter chroococcum gene for 16S rRNA, partial sequence	AB175653	1430	nuccore	353
20218	Azotobacter chroococcum strain LMG 8756 16S ribosomal RNA gene, partial sequence	EU401906	1501	nuccore	353
20218	Azotobacter chroococcum gene for 16S rRNA, partial sequence, strain: NBRC 102613	AB681887	1461	nuccore	353

Genome sequences

@ref	description	accession	assembly level	database	NCBI tax ID
66792	Azotobacter chroococcum strain ATCC 9043	353.12	wgs	patric	353
66792	Azotobacter chroococcum strain DSM 2286	353.14	wgs	patric	353
66792	Azotobacter chroococcum DSM 2286	2802428852	draft	img	353
67770	Azotobacter chroococcum ATCC 9043	GCA_004327905	scaffold	ncbi	353
67770	Azotobacter chroococcum DSM 2286	GCA_004339665	scaffold	ncbi	353

Genome-based predictions

predictions

@ref	model	trait	description	prediction	confidence	training_data
125438	gram-positive	gram-positive	Positive reaction to Gram-staining	no	98.5	no
125438	anaerobic	anaerobic	Ability to grow under anoxygenic conditions (including facultative anaerobes)	no	95.169	no
125438	spore-forming	spore-forming	Ability to form endo- or exospores	no	85.924	no
125438	aerobic	aerobic	Ability to grow under oxygenic conditions (including facultative aerobes)	yes	82.514	no
125438	thermophile	thermophilic	Ability to grow at temperatures above or equal to 45°C	no	96.8	no
125438	motile2+	flagellated	Ability to perform flagellated movement	yes	87.686	no
125439	BacteriaNet	spore_formation	Ability to form endo- or exospores	no	99
125439	BacteriaNet	motility	Ability to perform movement	yes	87.9
125439	BacteriaNet	gram_stain	Reaction to gram-staining	negative	94.4
125439	BacteriaNet	oxygen_tolerance	Oxygenic conditions needed for growth	facultative aerobe	70.4

External links

@ref: 1019

culture collection no.: DSM 2286, ATCC 9043, VKM B-1616, CCUG 53675, LMG 8756, CECT 4103, IAM 12666, IAM 15040, JCM 20725, BCRC 10599, ICMP 15214, JCM 21503, NBRC 102613, NCAIM B.01391, NCIMB 11694, NRRL B-14346, NRRL B-14637

straininfo link

@ref: 81945
straininfo: 1078

literature

topic	Pubmed-ID	title	authors	journal	DOI	year	mesh	topic2
Enzymology	1987127	Detection of alternative nitrogenases in aerobic gram-negative nitrogen-fixing bacteria.	Fallik E, Chan YK, Robson RL	J Bacteriol	10.1128/jb.173.1.365-371.1991	1991	Azotobacter/enzymology/genetics, DNA Probes, DNA, Bacterial/genetics/isolation & purification, Escherichia coli/genetics, Genes, Bacterial, Gram-Negative Aerobic Bacteria/enzymology/genetics, Nitrogen Fixation, Nitrogenase/genetics, Nucleic Acid Hybridization, Pseudomonas/enzymology/genetics, Rhizobium/enzymology/genetics, Species Specificity	Phylogeny
Metabolism	8415713	Effects of Mn2+ and Mg2+ on assimilation of NO3- and NH4+ by soil microorganisms.	McCarty GW, Bremner JM	Proc Natl Acad Sci U S A	10.1073/pnas.90.20.9403	1993	Azotobacter/metabolism, Magnesium/pharmacology, Manganese/pharmacology, Nitrates/metabolism, Pseudomonas/metabolism, Quaternary Ammonium Compounds/metabolism, Soil Microbiology
Phylogeny	31046894	Azotobacter bryophylli sp. nov., isolated from the succulent plant Bryophyllum pinnatum.	Liu L, Yuan T, An Q, Yang M, Mao X, Mo C, Tan Z, Peng G	Int J Syst Evol Microbiol	10.1099/ijsem.0.003412	2019	Azotobacter/classification/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA Fingerprinting, DNA, Bacterial/genetics, Fatty Acids/chemistry, Kalanchoe/microbiology, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Plant Leaves/microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Ubiquinone/chemistry	Transcriptome
Phylogeny	31995466	Description of Azotobacter chroococcum subsp. isscasi subsp. nov. isolated from paddy soil and establishment of Azotobacter chroococcum subsp. chroococcum subsp. nov.	Jin H, Wang H, Zhang Y, Hu T, Lin Z, Liu B, Ma J, Wang X, Liu Q, Lin X, Xie Z	Int J Syst Evol Microbiol	10.1099/ijsem.0.004026	2020	Azotobacter/classification/isolation & purification, Bacterial Typing Techniques, Base Composition, Base Sequence, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Oryza, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology	Transcriptome
Metabolism	33436213	Characterization of a novel endo-levanase from Azotobacter chroococcum DSM 2286 and its application for the production of prebiotic fructooligosaccharides.	Hovels M, Kosciow K, Deppenmeier U	Carbohydr Polym	10.1016/j.carbpol.2020.117384	2020	Azotobacter/enzymology/genetics, Bacterial Proteins/genetics/metabolism, Disaccharides/chemistry/metabolism, Escherichia coli/enzymology/genetics, Fructans/chemistry/metabolism, Fructose/chemistry/metabolism, Gene Expression, Gluconobacter/enzymology/genetics, Glycoside Hydrolases/genetics/metabolism, Hexosyltransferases/genetics/metabolism, Humans, Hydrolysis, Oligosaccharides/biosynthesis/chemistry, Phleum/chemistry, Prebiotics/*analysis, Recombinant Proteins/genetics/metabolism, Sucrose/chemistry/metabolism	Enzymology

Reference

@id	authors	catalogue	doi/url	title	journal	pubmed
1019	Curators of the DSMZ	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2286)	https://www.dsmz.de/collection/catalogue/details/culture/DSM-2286
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.		10.1099/ijsem.0.004332	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.		10.1016/j.syapm.2013.11.002	StrainInfo introduces electronic passports for microorganisms.	Syst Appl Microbiol. 37: 42-50 2014	24321274
66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770	Curators of the JCM		https://jcm.brc.riken.jp/en/
81945	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.		10.60712/SI-ID1078.1	StrainInfo: A central database for resolving microbial strain identifiers
125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann		10.1101/2024.08.12.607695	Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets
125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy		https://github.com/GenomeNet/deepG	deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1