Strain identifier
BacDive ID: 12720
Type strain: 
Species: Azomonas macrocytogenes
Strain history: CIP <- 1993, ATCC <- D.B. Johnstone, Azotobacter macrocytogenes
NCBI tax ID(s): 69962 (species)
version 9.3 (current version)
General
@ref: 367
BacDive-ID: 12720
DSM-Number: 721
keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-negative
description: Azomonas macrocytogenes 0 is a mesophilic, Gram-negative bacterium that was isolated from garden soil.
NCBI tax id
- NCBI tax id: 69962
- Matching level: species
strain history
| @ref | history |
|---|---|
| 367 | <- J. De Ley <- NCIB <- H.L. Jensen, 0 (Azotobacter macrocytogenes) |
| 120742 | CIP <- 1993, ATCC <- D.B. Johnstone, Azotobacter macrocytogenes |
doi: 10.13145/bacdive12720.20250331.9.3
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Pseudomonadales
- family: Pseudomonadaceae
- genus: Azomonas
- species: Azomonas macrocytogenes
- full scientific name: Azomonas macrocytogenes (Jensen 1955) New and Tchan 1982
synonyms
@ref synonym 20215 Azomonotrichon macrocytogenes 20215 Azotobacter macrocytogenes
@ref: 367
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Pseudomonadales
family: Pseudomonadaceae
genus: Azomonas
species: Azomonas macrocytogenes
full scientific name: Azomonas macrocytogenes (Jensen 1955) New and Tchan 1982
strain designation: 0
type strain: yes
Morphology
cell morphology
| @ref | gram stain | confidence | motility |
|---|---|---|---|
| 125439 | negative | 99.9 | |
| 125439 | 91.2 | yes | |
| 125438 | negative | 98.985 |
Culture and growth conditions
culture medium
| @ref | name | growth | composition | link |
|---|---|---|---|---|
| 40875 | MEDIUM 29- Brain heart agar | yes | Distilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g) | |
| 367 | DIAZOTROPHIC MEDIUM (RBA) (DSMZ Medium 441) | yes | Name: DIAZOTROPHIC MEDIUM (RBA) (DSMZ Medium 441) Composition: Agar 14.881 g/l DL-Malate 1.98413 g/l D-Glucose 1.98413 g/l D-Mannitol 1.98413 g/l Na-pyruvate 0.992063 g/l Disodium succinate 0.992063 g/l K2HPO4 0.892857 g/l KH2PO4 0.0992065 g/l NaCl 0.0992065 g/l CaCl2 x 2 H2O 0.0992065 g/l MgSO4 x 7 H2O 0.0992065 g/l Yeast extract 0.0496032 g/l FeSO4 x 7 H2O 0.00992065 g/l MnSO4 x H2O 0.00496032 g/l NaVO3 x H2O 0.00496032 g/l Nicotinic acid 0.00248016 g/l Pyridoxine hydrochloride 0.00248016 g/l Calcium pantothenate 0.00248016 g/l Thiamine-HCl x 2 H2O 0.00248016 g/l H3BO3 0.000892857 g/l CoCl2 x 6 H2O 0.000595238 g/l Riboflavin 0.000496032 g/l ZnSO4 x 7 H2O 0.000297619 g/l MnCl2 x 4 H2O 8.92857e-05 g/l Na2MoO4 x 2 H2O 8.92857e-05 g/l NiCl2 x 6 H2O 5.95238e-05 g/l Vitamin B12 4.96032e-05 g/l CuCl2 x 2 H2O 2.97619e-05 g/l Folic acid 9.92063e-06 g/l Biotin 4.96032e-06 g/l Distilled water | https://mediadive.dsmz.de/medium/441 |
| 367 | AZOTOBACTER MEDIUM (DSMZ Medium 3) | yes | Name: AZOTOBACTER MEDIUM (DSMZ Medium 3) Composition: Agar 15.0 g/l CaCO3 5.0 g/l Glucose 5.0 g/l Mannitol 5.0 g/l K2HPO4 0.9 g/l KH2PO4 0.1 g/l MgSO4 x 7 H2O 0.1 g/l CaCl2 x 2 H2O 0.1 g/l FeSO4 x 7 H2O 0.01 g/l Na2MoO4 x 2 H2O 0.005 g/l Distilled water | https://mediadive.dsmz.de/medium/3 |
| 120742 | CIP Medium 29 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29 |
culture temp
| @ref | growth | type | temperature |
|---|---|---|---|
| 367 | positive | growth | 28 |
| 40875 | positive | growth | 26 |
Physiology and metabolism
spore formation
- @ref: 125439
- spore formation: no
- confidence: 99.8
Isolation, sampling and environmental information
isolation
| @ref | sample type | country | origin.country | continent |
|---|---|---|---|---|
| 367 | garden soil | Denmark | DNK | Europe |
| 59971 | Soil,garden | |||
| 120742 | Environment, Soil | Denmark | DNK | Europe |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Terrestrial | #Soil |
| #Engineered | #Agriculture | #Garden |
Safety information
risk assessment
| @ref | biosafety level | biosafety level comment |
|---|---|---|
| 367 | 1 | Risk group (German classification) |
| 120742 | 1 | Risk group (French classification) |
Sequence information
16S sequences
- @ref: 20218
- description: Azomonas macrocytogenes gene for 16S rRNA, partial sequence
- accession: AB175654
- length: 1442
- database: nuccore
- NCBI tax ID: 69962
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Azomonas macrocytogenes CECT 4462 | GCA_014191985 | scaffold | ncbi | 69962 |
| 66792 | Azomonas macrocytogenes strain CECT 4462 | 69962.3 | wgs | patric | 69962 |
| 66792 | Azomonas macrocytogenes CECT 4462 | 2824346434 | draft | img | 69962 |
GC content
- @ref: 367
- GC-content: 59.2
Genome-based predictions
predictions
| @ref | model | trait | description | prediction | confidence | training_data |
|---|---|---|---|---|---|---|
| 125438 | gram-positive | gram-positive | Positive reaction to Gram-staining | no | 98.985 | no |
| 125438 | anaerobic | anaerobic | Ability to grow under anoxygenic conditions (including facultative anaerobes) | no | 89.14 | no |
| 125438 | aerobic | aerobic | Ability to grow under oxygenic conditions (including facultative aerobes) | yes | 72.678 | no |
| 125438 | spore-forming | spore-forming | Ability to form endo- or exospores | no | 87.343 | no |
| 125438 | thermophile | thermophilic | Ability to grow at temperatures above or equal to 45°C | no | 96.829 | yes |
| 125438 | motile2+ | flagellated | Ability to perform flagellated movement | yes | 86.436 | no |
| 125439 | BacteriaNet | spore_formation | Ability to form endo- or exospores | no | 99.8 | |
| 125439 | BacteriaNet | motility | Ability to perform movement | yes | 91.2 | |
| 125439 | BacteriaNet | gram_stain | Reaction to gram-staining | negative | 99.9 | |
| 125439 | BacteriaNet | oxygen_tolerance | Oxygenic conditions needed for growth | obligate aerobe | 63.9 |
External links
@ref: 367
culture collection no.: CCUG 53671, CIP 103928, LMG 1266, CECT 4462, IAM 15003, LMG 8755, DSM 721, ATCC 12335, NCIB 8700, NCIMB 8700
straininfo link
- @ref: 81929
- straininfo: 1016
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 367 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 721) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-721 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 40875 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/15780 | ||||
| 59971 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 53671) | https://www.ccug.se/strain?id=53671 | |||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 81929 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID1016.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
| 120742 | Curators of the CIP | Collection of Institut Pasteur (CIP 103928) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103928 | |||
| 125438 | Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann | 10.1101/2024.08.12.607695 | Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets | |||
| 125439 | Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy | https://github.com/GenomeNet/deepG | deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 |