Strain identifier
BacDive ID: 12707
Type strain:
Species: Azorhizophilus paspali
Strain Designation: Ax-8, WR-129
Strain history: IAM 12667 <-- ATCC 23833 <-- D. B. Johnstone <-- J. Döbereiner Ax-8.
NCBI tax ID(s): 69963 (species)
General
@ref: 1017
BacDive-ID: 12707
DSM-Number: 2283
keywords: 16S sequence, Bacteria, mesophilic
description: Azorhizophilus paspali Ax-8 is a mesophilic bacterium that was isolated from rhizosphere soil of Paspalum notatum.
NCBI tax id
- NCBI tax id: 69963
- Matching level: species
strain history
@ref | history |
---|---|
1017 | <- WR, WR-129 <- J. Döbereiner, Ax-8 |
67770 | IAM 12667 <-- ATCC 23833 <-- D. B. Johnstone <-- J. Döbereiner Ax-8. |
doi: 10.13145/bacdive12707.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Pseudomonadales
- family: Pseudomonadaceae
- genus: Azorhizophilus
- species: Azorhizophilus paspali
- full scientific name: Azorhizophilus paspali (Döbereiner 1966) Thompson and Skerman 1981
synonyms
- @ref: 20215
- synonym: Azotobacter paspali
@ref: 1017
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Pseudomonadales
family: Pseudomonadaceae
genus: Azorhizophilus
species: Azorhizophilus paspali
full scientific name: Azorhizophilus paspali (Döbereiner 1966) Thompson and Skerman 1981
strain designation: Ax-8, WR-129
type strain: yes
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
1017 | DIAZOTROPHIC MEDIUM (RBA) (DSMZ Medium 441) | yes | https://mediadive.dsmz.de/medium/441 | Name: DIAZOTROPHIC MEDIUM (RBA) (DSMZ Medium 441) Composition: Agar 14.881 g/l DL-Malate 1.98413 g/l D-Glucose 1.98413 g/l D-Mannitol 1.98413 g/l Na-pyruvate 0.992063 g/l Disodium succinate 0.992063 g/l K2HPO4 0.892857 g/l KH2PO4 0.0992065 g/l NaCl 0.0992065 g/l CaCl2 x 2 H2O 0.0992065 g/l MgSO4 x 7 H2O 0.0992065 g/l Yeast extract 0.0496032 g/l FeSO4 x 7 H2O 0.00992065 g/l MnSO4 x H2O 0.00496032 g/l NaVO3 x H2O 0.00496032 g/l Nicotinic acid 0.00248016 g/l Pyridoxine hydrochloride 0.00248016 g/l Calcium pantothenate 0.00248016 g/l Thiamine-HCl x 2 H2O 0.00248016 g/l H3BO3 0.000892857 g/l CoCl2 x 6 H2O 0.000595238 g/l Riboflavin 0.000496032 g/l ZnSO4 x 7 H2O 0.000297619 g/l MnCl2 x 4 H2O 8.92857e-05 g/l Na2MoO4 x 2 H2O 8.92857e-05 g/l NiCl2 x 6 H2O 5.95238e-05 g/l Vitamin B12 4.96032e-05 g/l CuCl2 x 2 H2O 2.97619e-05 g/l Folic acid 9.92063e-06 g/l Biotin 4.96032e-06 g/l Distilled water |
1017 | AZOTOBACTER MEDIUM (DSMZ Medium 3) | yes | https://mediadive.dsmz.de/medium/3 | Name: AZOTOBACTER MEDIUM (DSMZ Medium 3) Composition: Agar 15.0 g/l CaCO3 5.0 g/l Glucose 5.0 g/l Mannitol 5.0 g/l K2HPO4 0.9 g/l KH2PO4 0.1 g/l MgSO4 x 7 H2O 0.1 g/l CaCl2 x 2 H2O 0.1 g/l FeSO4 x 7 H2O 0.01 g/l Na2MoO4 x 2 H2O 0.005 g/l Distilled water |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
1017 | positive | growth | 30 | mesophilic |
67770 | positive | growth | 30 | mesophilic |
Isolation, sampling and environmental information
isolation
@ref | sample type | host species | country | origin.country | continent |
---|---|---|---|---|---|
1017 | rhizosphere soil of Paspalum notatum | Paspalum notatum | Brazil | BRA | Middle and South America |
59973 | Soil,rhizosphere | ||||
67770 | Soil |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Terrestrial | #Soil |
#Host | #Plants | #Herbaceous plants (Grass,Crops) |
#Host Body-Site | #Plant | #Rhizosphere |
taxonmaps
- @ref: 69479
- File name: preview.99_177588.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_23;96_646;97_1313;98_1582;99_177588&stattab=map
- Last taxonomy: Azotobacter
- 16S sequence: AB681742
- Sequence Identity:
- Total samples: 37
- soil counts: 9
- aquatic counts: 10
- animal counts: 15
- plant counts: 3
Safety information
risk assessment
- @ref: 1017
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Azorhizophilus paspali partial 16S rRNA gene, isolate ICMP 7469; ATCC 23833 | AJ308318 | 1384 | ena | 69963 |
20218 | Azorhizophilus paspali strain ICMP 7469 16S ribosomal RNA gene, partial sequence | EF100147 | 1371 | ena | 69963 |
20218 | Azorhizophilus paspali gene for 16S rRNA, partial sequence, strain: NBRC 102228 | AB681742 | 1461 | ena | 69963 |
External links
@ref: 1017
culture collection no.: DSM 2283, ATCC 23833, CCUG 53674, CECT 4095, LMG 3864, IAM 12667, ICMP 7469, JCM 20726, NBRC 102228, NCIMB 12095
straininfo link
- @ref: 81917
- straininfo: 1025
literature
- topic: Enzymology
- Pubmed-ID: 1987127
- title: Detection of alternative nitrogenases in aerobic gram-negative nitrogen-fixing bacteria.
- authors: Fallik E, Chan YK, Robson RL
- journal: J Bacteriol
- DOI: 10.1128/jb.173.1.365-371.1991
- year: 1991
- mesh: Azotobacter/enzymology/genetics, DNA Probes, DNA, Bacterial/genetics/isolation & purification, Escherichia coli/genetics, *Genes, Bacterial, Gram-Negative Aerobic Bacteria/enzymology/*genetics, *Nitrogen Fixation, Nitrogenase/*genetics, Nucleic Acid Hybridization, Pseudomonas/enzymology/genetics, Rhizobium/enzymology/genetics, Species Specificity
- topic2: Phylogeny
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
1017 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2283) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-2283 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
59973 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 53674) | https://www.ccug.se/strain?id=53674 | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
81917 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID1025.1 | StrainInfo: A central database for resolving microbial strain identifiers |