Strain identifier
BacDive ID: 12696
Type strain:
Species: Pseudoalteromonas issachenkonii
Strain history: CIP <- 2000, E.P. Ivanova, Russian Academy Sci., Vladivostok, Russia
NCBI tax ID(s): 1315274 (strain), 152297 (species)
General
@ref: 6188
BacDive-ID: 12696
DSM-Number: 15925
keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, psychrophilic, Gram-negative, motile, rod-shaped
description: Pseudoalteromonas issachenkonii DSM 15925 is an obligate aerobe, psychrophilic, Gram-negative bacterium that was isolated from thallus of the brown alga Fucus evanescens.
NCBI tax id
NCBI tax id | Matching level |
---|---|
152297 | species |
1315274 | strain |
strain history
@ref | history |
---|---|
6188 | <- E. P. Ivanova, Swinburne Univ. Technol., IRIS, Hawthorn, Australia; KMM 3549 |
42039 | 2000, E.P. Ivanova, Russian Academy Sci., Vladivostok, Russia. |
120288 | CIP <- 2000, E.P. Ivanova, Russian Academy Sci., Vladivostok, Russia |
doi: 10.13145/bacdive12696.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Alteromonadales
- family: Pseudoalteromonadaceae
- genus: Pseudoalteromonas
- species: Pseudoalteromonas issachenkonii
- full scientific name: Pseudoalteromonas issachenkonii Ivanova et al. 2002
@ref: 6188
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Alteromonadales
family: Pseudoalteromonadaceae
genus: Pseudoalteromonas
species: Pseudoalteromonas issachenkonii
full scientific name: Pseudoalteromonas issachenkonii Ivanova et al. 2002
type strain: yes
Morphology
cell morphology
- @ref: 120288
- gram stain: negative
- cell shape: rod-shaped
- motility: yes
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
6188 | BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) | yes | https://mediadive.dsmz.de/medium/514 | Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water |
42039 | Marine agar (MA) | yes | Distilled water make up to (1000.000 ml);Marine agar (55.100 g) | |
120288 | CIP Medium 13 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
6188 | positive | growth | 22 | psychrophilic |
42039 | positive | growth | 25 | mesophilic |
120288 | positive | growth | 5-37 | |
120288 | no | growth | 41 | thermophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 120288
- oxygen tolerance: obligate aerobe
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
120288 | NaCl | positive | growth | 2-10 % |
120288 | NaCl | no | growth | 0 % |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
120288 | 4853 | esculin | + | hydrolysis |
120288 | 17632 | nitrate | + | reduction |
120288 | 16301 | nitrite | - | reduction |
120288 | 17632 | nitrate | - | respiration |
antibiotic resistance
- @ref: 120288
- metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
- is antibiotic: yes
- is sensitive: no
- is resistant: yes
metabolite production
- @ref: 120288
- Chebi-ID: 35581
- metabolite: indole
- production: no
enzymes
@ref | value | activity | ec |
---|---|---|---|
120288 | oxidase | + | |
120288 | beta-galactosidase | - | 3.2.1.23 |
120288 | alcohol dehydrogenase | - | 1.1.1.1 |
120288 | gelatinase | + | |
120288 | amylase | - | |
120288 | caseinase | + | 3.4.21.50 |
120288 | catalase | + | 1.11.1.6 |
120288 | tween esterase | + | |
120288 | lecithinase | + | |
120288 | lipase | + | |
120288 | lysine decarboxylase | - | 4.1.1.18 |
120288 | ornithine decarboxylase | - | 4.1.1.17 |
120288 | protease | + | |
120288 | tryptophan deaminase | - | |
120288 | urease | - | 3.5.1.5 |
68382 | alkaline phosphatase | + | 3.1.3.1 |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | + | |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | + | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | + | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
120288 | - | + | + | + | - | + | + | - | + | - | + | + | - | - | - | - | - | - | - | - |
API biotype100
@ref | GLU | FRU | GAL | TRE | MNE | SBE | MEL | SAC | RAF | MTE | MAL | LAC | LTE | MbGa | MaGa | CEL | GEN | MbGu | ESC | RIB | ARA | XYL | PLE | RHA | FUC | MLZ | DARL | LARL | XLT | DUL | TAG | GLY | INO | MAN | MTL | TUR | SOR | ADO | HBG | LYX | ERY | MDG | 3MDG | SAT | MUC | LTAT | DTAT | MTAT | DMLT | LMLT | CATE | TATE | TTE | CIT | GRT | GAT | 2KG | 5KG | TRY | NAG | GNT | PAC | PAT | pOBE | QAT | GTE | mOBE | BAT | PPAT | CMT | TGE | BET | PCE | ABT | HIN | LAT | CAP | CYT | HIS | SUC | FUM | GRE | GYT | AVT | ETN | TTN | GLN | ITA | 3OBU | APT | GTT | PRO | DALA | LALA | SER | MNT | PROP | TYR | 2KT |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
120288 | + | - | - | + | - | - | - | + | - | + | + | - | - | - | - | - | - | - | + | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | + | - | - | - | - | - | + | - | + | - | - | + | - | + | + | - | + | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | host species | geographic location | country | origin.country | continent | isolation date |
---|---|---|---|---|---|---|---|
6188 | thallus of the brown alga Fucus evanescens | Fucus evanescens | Pacific Ocean, Kurile Islands (Kraternaya Bight) | Russia | RUS | Asia | |
120288 | Plant, Alga Fucus evanescens | Kraternaya bight, Kuril Islands | Russian Federation | RUS | Europe | 1999 |
isolation source categories
- Cat1: #Host
- Cat2: #Algae
- Cat3: #Brown Algae
taxonmaps
- @ref: 69479
- File name: preview.99_1002.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_57;96_122;97_129;98_145;99_1002&stattab=map
- Last taxonomy: Pseudoalteromonas
- 16S sequence: AF316144
- Sequence Identity:
- Total samples: 3222
- soil counts: 24
- aquatic counts: 2792
- animal counts: 384
- plant counts: 22
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
6188 | 1 | Risk group (German classification) |
120288 | 2 | Risk group (French classification) |
Sequence information
16S sequences
- @ref: 6188
- description: Pseudoalteromonas issachenkonii strain KMM 3549 16S ribosomal RNA gene, partial sequence
- accession: AF316144
- length: 1446
- database: ena
- NCBI tax ID: 152297
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Pseudoalteromonas issachenkonii KMM 3549 | GCA_002310795 | complete | ncbi | 152297 |
66792 | Pseudoalteromonas issachenkonii strain KMM 3549 | 152297.5 | complete | patric | 152297 |
66792 | Pseudoalteromonas issachenkonii KMM 3549 | 2802429664 | complete | img | 1315274 |
External links
@ref: 6188
culture collection no.: DSM 15925, CIP 106858, LMG 19697, KMM 3549
straininfo link
- @ref: 81908
- straininfo: 13467
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 11837307 | Pseudoalteromonas issachenkonii sp. nov., a bacterium that degrades the thallus of the brown alga Fucus evanescens. | Ivanova EP, Sawabe T, Alexeeva YV, Lysenko AM, Gorshkova NM, Hayashi K, Zukova NV, Christen R, Mikhailov VV | Int J Syst Evol Microbiol | 10.1099/00207713-52-1-229 | 2002 | Gammaproteobacteria/*classification/*genetics/metabolism, Genes, rRNA, Molecular Sequence Data, Phaeophyta/metabolism/*microbiology, Phenotype, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater, Sequence Analysis, DNA | Metabolism |
Cultivation | 12358700 | Optimization of glycosidases production by Pseudoalteromonas issachenkonii KMM 3549(T). | Alexeeva YV, Ivanova EP, Bakunina IY, Zvaygintseva TN, Mikhailov VV | Lett Appl Microbiol | 10.1046/j.1472-765x.2002.01189.x | 2002 | Culture Media, Glucan Endo-1,3-beta-D-Glucosidase, Glycoside Hydrolases/*biosynthesis, *Polysaccharide-Lyases, Polysaccharides/*metabolism, Pseudoalteromonas/*enzymology/growth & development, Xylose/administration & dosage/*metabolism | Metabolism |
Cultivation | 14687213 | Impact of cultivation conditions on haemolytic activity of Pseudoalteromonas issachenkonii KMM 3549T. | Alexeeva YV, Kalinovskaya NI, Kuznetsova TA, Ivanova EP | Lett Appl Microbiol | 10.1046/j.1472-765x.2003.01440.x | 2004 | Animals, Bacterial Proteins/biosynthesis, Calcium Chloride, Chymotrypsin, Culture Media/chemistry, Edetic Acid, Erythrocytes/metabolism, Hemolysin Proteins/*biosynthesis, *Hemolysis, Humans, Iron, Mice, Pseudoalteromonas/*enzymology/*physiology, Rabbits, Temperature | Pathogenicity |
Phylogeny | 15261592 | The complete structure of the lipooligosaccharide from the halophilic bacterium Pseudoalteromonas issachenkonii KMM 3549T. | Silipo A, Leone S, Lanzetta R, Parrilli M, Sturiale L, Garozzo D, Nazarenko EL, Gorshkova RP, Ivanova EP, Gorshkova NM, Molinaro A | Carbohydr Res | 10.1016/j.carres.2004.05.008 | 2004 | Bacillaceae/*chemistry, Carbohydrate Conformation, Carbohydrate Sequence, Lipopolysaccharides/*chemistry/isolation & purification, Magnetic Resonance Spectroscopy, Molecular Sequence Data, Pseudoalteromonas/*chemistry/classification, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods, Substrate Specificity | Genetics |
Genetics | 26732413 | Complete genome of brown algal polysaccharides-degrading Pseudoalteromonas issachenkonii KCTC 12958(T) (=KMM 3549(T)). | Lee SH, Choe H, Kim SG, Park DS, Nasir A, Kim BK, Kim KM | J Biotechnol | 10.1016/j.jbiotec.2015.12.031 | 2015 | Base Composition, Fucus/microbiology, Genome Size, *Genome, Bacterial, Polysaccharides/*metabolism, Pseudoalteromonas/*genetics/isolation & purification/metabolism | Metabolism |
Reference
@id | authors | catalogue | doi/url | title |
---|---|---|---|---|
6188 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15925) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-15925 | |
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
42039 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/19035 | ||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
68382 | Automatically annotated from API zym | |||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |
81908 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID13467.1 | StrainInfo: A central database for resolving microbial strain identifiers | |
120288 | Curators of the CIP | Collection of Institut Pasteur (CIP 106858) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106858 |