Strain identifier

BacDive ID: 12687

Type strain: Yes

Species: Pseudoalteromonas peptidolytica

Strain Designation: F12-50-A1, F12 50-A1

Strain history: CIP <- 2001, A. Katsuta, Marine Biotechnol. Inst., Kamaishi, Iwate, Japan <- Dohmoto: strain F12 50-A1

NCBI tax ID(s): 61150 (species)

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General

@ref: 5213

BacDive-ID: 12687

DSM-Number: 14001

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, motile

description: Pseudoalteromonas peptidolytica F12-50-A1 is a mesophilic, motile bacterium that was isolated from sea water.

NCBI tax id

  • NCBI tax id: 61150
  • Matching level: species

strain history

@refhistory
5213<- A. Katsuta <- N. Dohmoto; F12-50-A1
121669CIP <- 2001, A. Katsuta, Marine Biotechnol. Inst., Kamaishi, Iwate, Japan <- Dohmoto: strain F12 50-A1

doi: 10.13145/bacdive12687.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Alteromonadales
  • family: Pseudoalteromonadaceae
  • genus: Pseudoalteromonas
  • species: Pseudoalteromonas peptidolytica
  • full scientific name: Pseudoalteromonas peptidolytica Venkateswaran and Dohmoto 2000

@ref: 5213

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Alteromonadales

family: Pseudoalteromonadaceae

genus: Pseudoalteromonas

species: Pseudoalteromonas peptidolytica

full scientific name: Pseudoalteromonas peptidolytica Venkateswaran and Dohmoto 2000

strain designation: F12-50-A1, F12 50-A1

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480yes98.034
6948099.986negative
121669nonegativerod-shaped

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5213BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
42019Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
121669CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperaturerange
5213positivegrowth28mesophilic
42019positivegrowth30mesophilic
121669positivegrowth25-41
121669nogrowth5psychrophilic
121669nogrowth10psychrophilic

Physiology and metabolism

spore formation

@refspore formationconfidence
69481no100
69480no99.637

halophily

@refsaltgrowthtested relationconcentration
121669NaClpositivegrowth2-8 %
121669NaClnogrowth0 %
121669NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1216694853esculin+hydrolysis
12166917632nitrate-reduction
12166916301nitrite-reduction

metabolite production

  • @ref: 121669
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
121669oxidase+
121669beta-galactosidase-3.2.1.23
121669alcohol dehydrogenase-1.1.1.1
121669gelatinase+
121669amylase+
121669caseinase+3.4.21.50
121669catalase+1.11.1.6
121669tween esterase+
121669lecithinase+
121669lipase+
121669lysine decarboxylase-4.1.1.18
121669ornithine decarboxylase-4.1.1.17
121669protease+
121669tryptophan deaminase-
121669urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121669-+++-+----++-----+--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
5213sea waterThe sea of JapanJapanJPNAsia
121669Environment, SeawaterJapanJPNAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Marine

taxonmaps

  • @ref: 69479
  • File name: preview.99_6623.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_57;96_353;97_393;98_4884;99_6623&stattab=map
  • Last taxonomy: Pseudoalteromonas
  • 16S sequence: AB681334
  • Sequence Identity:
  • Total samples: 1509
  • soil counts: 33
  • aquatic counts: 1221
  • animal counts: 253
  • plant counts: 2

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
52131Risk group (German classification)
1216691Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Pseudoalteromonas peptidolytica gene for 16S rRNA, partial sequence, strain: NBRC 101021AB6813341459ena61150
5213Pseudoalteromonas peptidysin 16S ribosomal RNA gene, partial sequenceAF0072861448ena61150

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pseudoalteromonas peptidolytica DSM 14001GCA_012641745contigncbi61150
66792Pseudoalteromonas peptidolytica F12-50-A1GCA_014858745contigncbi1315280
66792Pseudoalteromonas peptidolytica F12-50-A11315280.3wgspatric1315280
66792Pseudoalteromonas peptidolytica strain DSM 1400161150.5wgspatric61150

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileyes92.094no
gram-positiveno99.12no
anaerobicno96.744no
halophileyes63.656no
spore-formingno93.809no
glucose-utilyes89.265no
flagellatedyes93.155no
thermophileno99.136no
aerobicyes85.682no
glucose-fermentno74.847no

External links

@ref: 5213

culture collection no.: DSM 14001, MBIC 1416, NITE 00156, CIP 107066, MBIC 01416

straininfo link

  • @ref: 81899
  • straininfo: 100877

literature

  • topic: Phylogeny
  • Pubmed-ID: 10758862
  • title: Pseudoalteromonas peptidolytica sp. nov., a novel marine mussel-thread-degrading bacterium isolated from the Sea of Japan.
  • authors: Venkateswaran K, Dohmoto N
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/00207713-50-2-565
  • year: 2000
  • mesh: Animals, Biodegradation, Environmental, Bivalvia/chemistry/*metabolism, DNA Gyrase, DNA Primers, DNA Topoisomerases, Type II/genetics, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/analysis, Gammaproteobacteria/*classification/genetics/isolation & purification/physiology, Gram-Negative Aerobic Bacteria/*classification/genetics/isolation & purification/physiology, Japan, Molecular Sequence Data, Nucleic Acid Hybridization, Peptides/chemical synthesis/metabolism, Phylogeny, Polymerase Chain Reaction, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Water Microbiology
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5213Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14001)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14001
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
42019Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/19265
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
81899Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID100877.1StrainInfo: A central database for resolving microbial strain identifiers
121669Curators of the CIPCollection of Institut Pasteur (CIP 107066)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107066