Strain identifier

BacDive ID: 12686

Type strain: Yes

Species: Pseudoalteromonas tetraodonis

Strain Designation: GFC

Strain history: CIP <- 1996, NCIMB <- IAM <- U. Simidu: strain GFC

NCBI tax ID(s): 1315271 (strain), 43659 (species)

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General

@ref: 3499

BacDive-ID: 12686

DSM-Number: 9166

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, psychrophilic, motile, animal pathogen

description: Pseudoalteromonas tetraodonis GFC is an obligate aerobe, psychrophilic, motile animal pathogen that was isolated from skin slime of pufferfish .

NCBI tax id

NCBI tax idMatching level
43659species
1315271strain

strain history

@refhistory
3499<- NCIMB <- IAM <- U. Simidu
67770IAM 14160 <-- U. Simidu strain GFC <-- T. Yasumoto and M. Yotsu.
123491CIP <- 1996, NCIMB <- IAM <- U. Simidu: strain GFC

doi: 10.13145/bacdive12686.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Alteromonadales
  • family: Pseudoalteromonadaceae
  • genus: Pseudoalteromonas
  • species: Pseudoalteromonas tetraodonis
  • full scientific name: Pseudoalteromonas tetraodonis (Simidu et al. 1990) Ivanova et al. 2001

  • synonyms

    @refsynonym
    20215Pseudoalteromonas haloplanktis subsp. tetraodonis
    20215Alteromonas tetraodonis

@ref: 3499

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Alteromonadales

family: Pseudoalteromonadaceae

genus: Pseudoalteromonas

species: Pseudoalteromonas tetraodonis

full scientific name: Pseudoalteromonas tetraodonis (Simidu et al. 1990) Ivanova et al. 2001

strain designation: GFC

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480yes98.192
6948099.988negative
123491yesnegativerod-shaped

pigmentation

  • @ref: 123491
  • production: no
  • name: Pyocyanin

multimedia

  • @ref: 3499
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_9166.jpg
  • caption: Micrograph of DSM 9166. Scale bar represents 2 um.
  • intellectual property rights: Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3499BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
42032MEDIUM 192 - for Alteromonas rubrayesDistilled water make up to (250.000 ml);Agar (15.000 g);Yeast extract (3.000 g);Peptone (5.000 g);Synthetic sea solution - M0216 (750.000 ml)
123491CIP Medium 192yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=192
123491CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperaturerange
3499positivegrowth20psychrophilic
42032positivegrowth22psychrophilic
67770positivegrowth25mesophilic
123491positivegrowth10-30
123491nogrowth5psychrophilic
123491nogrowth37mesophilic
123491nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 123491
  • oxygen tolerance: obligate aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.956
123491no

halophily

@refsaltgrowthtested relationconcentration
123491NaClpositivegrowth2-10 %
123491NaClnogrowth0 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12349116947citrate-carbon source
1234914853esculin-hydrolysis
12349117632nitrate-reduction
12349116301nitrite-reduction
12349117632nitrate-respiration

antibiotic resistance

  • @ref: 123491
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
677709506tetrodotoxinyes
12349135581indoleno

enzymes

@refvalueactivityec
123491oxidase+
123491beta-galactosidase+3.2.1.23
123491alcohol dehydrogenase-1.1.1.1
123491gelatinase+
123491amylase+
123491DNase-
123491caseinase+3.4.21.50
123491catalase+1.11.1.6
123491tween esterase-
123491lecithinase-
123491lipase+
123491lysine decarboxylase-4.1.1.18
123491ornithine decarboxylase-4.1.1.17
123491protease+
123491tryptophan deaminase-
123491urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123491-+++-++---++--------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
123491+--+---+-++-----+-+------------------------------+---------+-------------------++-----+-+--+-++-++-

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinentisolation date
3499skin slime of pufferfish (Fugu poecilonotus)Fugu poecilonotus
67770Skin slime of pufferfish (Fugu poecilonotus)Fugu poecilonotus
123491Animal, Pufferfish (Fugu poecilonotus), skin slimeSendai BayJapanJPNAsia1985-07-01

isolation source categories

Cat1Cat2Cat3
#Host#Fishes
#Host Body-Site#Organ#Skin, Nail, Hair
#Host Body Product#Fluids

taxonmaps

  • @ref: 69479
  • File name: preview.99_1002.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_57;96_122;97_129;98_145;99_1002&stattab=map
  • Last taxonomy: Pseudoalteromonas
  • 16S sequence: X82139
  • Sequence Identity:
  • Total samples: 3222
  • soil counts: 24
  • aquatic counts: 2792
  • animal counts: 384
  • plant counts: 22

Safety information

risk assessment

@refpathogenicity animalbiosafety levelbiosafety level comment
3499yes1Risk group (German classification)
1234912Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Pseudoalteromonas tetraodonis strain IAM 14160 16S ribosomal RNA gene, partial sequenceAF2147301464ena43659
20218A.tetraodonis 16S rRNA gene (IAM 14160T)X821391428ena43659
20218Pseudoalteromonas tetraodonis strain KMM 458 16S ribosomal RNA gene, partial sequenceAF2147291491ena43659
20218Pseudoalteromonas tetraodonis gene for 16S rRNA, partial sequence, strain: NBRC 103034AB6819141459ena43659

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pseudoalteromonas tetraodonis GFCGCA_002310835completencbi43659
66792Pseudoalteromonas tetraodonis GFC NBRC 103034GCA_007991235contigncbi1315271
66792Pseudoalteromonas tetraodonis GFC strain NBRC 1030341315271.8wgspatric1315271
66792Pseudoalteromonas tetraodonis strain GFC43659.6completepatric43659

GC content

  • @ref: 67770
  • GC-content: 41
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedyes87.687no
gram-positiveno99.055no
anaerobicno95.547no
aerobicyes88.658no
halophileno55.745no
spore-formingno94.381no
glucose-utilyes89.994no
thermophileno98.967yes
motileyes91.904no
glucose-fermentno74.81no

External links

@ref: 3499

culture collection no.: DSM 9166, IAM 14160, NCIMB 13177, JCM 21038, ATCC 51193, CIP 104758, KMM 458, NBRC 103034, CIP 107120

straininfo link

  • @ref: 81898
  • straininfo: 42852

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny11411675Retrieval of the species Alteromonas tetraodonis Simidu et al. 1990 as Pseudoalteromonas tetraodonis comb. nov. and emendation of description.Ivanova EP, Romanenko LA, Matte MH, Matte GR, Lysenko AM, Simidu U, Kita-Tsukamoto K, Sawabe T, Vysotskii MV, Frolova GM, Mikhailov V, Christen R, Colwell RRInt J Syst Evol Microbiol10.1099/00207713-51-3-10712001Alteromonas/*classification/genetics, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Gammaproteobacteria/*classification/genetics, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, *Phylogeny, Polymerase Chain Reaction, RNA, Ribosomal, 16S/geneticsGenetics
Genetics11423109Structure of a colitose-containing O-specific polysaccharide of the marine bacterium Pseudoalteromonas tetraodonis IAM 14160(T).Muldoon J, Perepelov AV, Shashkov AS, Gorshkova RP, Nazarenko EL, Zubkov VA, Ivanova EP, Knirel YA, Savage AVCarbohydr Res10.1016/s0008-6215(01)00121-52001Carbohydrate Conformation, Carbohydrate Sequence, Deoxy Sugars/*chemistry, Gammaproteobacteria/*chemistry/isolation & purification, Magnetic Resonance Spectroscopy, Marine Biology, Molecular Sequence Data, O Antigens/*chemistryEnzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3499Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 9166)https://www.dsmz.de/collection/catalogue/details/culture/DSM-9166
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
42032Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16702
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
81898Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID42852.1StrainInfo: A central database for resolving microbial strain identifiers
123491Curators of the CIPCollection of Institut Pasteur (CIP 104758)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104758