Strain identifier

BacDive ID: 1268

Type strain: Yes

Species: Mesobacillus foraminis

Strain Designation: CV53

Strain history: CIP <- 2005, I. Tiago, Coimbra Univ., Coimbra, Portgual: strain CV53

NCBI tax ID(s): 279826 (species)

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General

@ref: 8209

BacDive-ID: 1268

DSM-Number: 19613

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped

description: Mesobacillus foraminis CV53 is an obligate aerobe, spore-forming, mesophilic bacterium that was isolated from alkaline groundwater.

NCBI tax id

  • NCBI tax id: 279826
  • Matching level: species

strain history

@refhistory
8209<- CIP <- I. Tiago, CV53
383572005, I. Tiago, Coimbra Univ., Coimbra, Portgual: strain CV53
67772Tiago, I. University of Coimbra
119424CIP <- 2005, I. Tiago, Coimbra Univ., Coimbra, Portgual: strain CV53

doi: 10.13145/bacdive1268.20250331.9.3

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Mesobacillus
  • species: Mesobacillus foraminis
  • full scientific name: Mesobacillus foraminis (Tiago et al. 2006) Patel and Gupta 2020

  • synonyms

    • @ref: 20215
    • synonym: Bacillus foraminis

@ref: 8209

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Mesobacillus

species: Mesobacillus foraminis

full scientific name: Mesobacillus foraminis (Tiago et al. 2006) Patel and Gupta 2020

strain designation: CV53

type strain: yes

Morphology

cell morphology

  • @ref: 119424
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 119424
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8209CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
38357MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
119424CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperature
8209positivegrowth30-37
38357positivegrowth37
67772positiveoptimum40
119424positivegrowth22-45
119424nogrowth10
119424nogrowth55

culture pH

@refabilitytypepHPH range
67772positivemaximum8.5alkaliphile
67772positiveminimum7.0

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
119424obligate aerobe
125439facultative anaerobe95.1

spore formation

@refspore formationconfidence
125438yes92.283
125439yes90.8

halophily

@refsaltgrowthtested relationconcentration
119424NaClnogrowth0 %
119424NaClnogrowth2 %
119424NaClnogrowth4 %
119424NaClnogrowth6 %
119424NaClnogrowth8 %
119424NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11942416947citrate-carbon source
1194244853esculin+hydrolysis
11942417632nitrate+reduction
11942416301nitrite-reduction
11942417632nitrate+respiration
6837159640N-acetylglucosamine+builds acid from
68371Potassium 2-ketogluconate+builds acid from
6837124265gluconate+builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose+builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose+builds acid from
6837128066gentiobiose+builds acid from
6837128087glycogen+builds acid from
6837128017starch+builds acid from
6837116634raffinose+builds acid from
683716731melezitose+builds acid from
6837115443inulin+builds acid from
6837127082trehalose+builds acid from
6837117992sucrose+builds acid from
6837128053melibiose+builds acid from
6837117716lactose+builds acid from
6837117306maltose+builds acid from
6837117057cellobiose+builds acid from
6837117814salicin+builds acid from
683714853esculin+builds acid from
6837118305arbutin+builds acid from
6837127613amygdalin+builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol+builds acid from
6837116899D-mannitol+builds acid from
6837117268myo-inositol+builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose+builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose+builds acid from
6837174863methyl beta-D-xylopyranoside+builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose+builds acid from
6837130849L-arabinose+builds acid from
6837117113erythritol-builds acid from
6837117754glycerol+builds acid from

metabolite production

  • @ref: 119424
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11942415688acetoin-
11942417234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-glucosidase+3.2.1.20
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase-3.1.3.2
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382lipase (C 14)-
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382alkaline phosphatase+3.1.3.1
119424oxidase+
119424beta-galactosidase+3.2.1.23
119424alcohol dehydrogenase-1.1.1.1
119424gelatinase-
119424amylase+
119424DNase+
119424caseinase-3.4.21.50
119424catalase+1.11.1.6
119424tween esterase-
119424gamma-glutamyltransferase+2.3.2.2
119424lecithinase-
119424lipase-
119424lysine decarboxylase-4.1.1.18
119424ornithine decarboxylase-4.1.1.17
119424protease-
119424urease-3.5.1.5
68382beta-glucosidase+3.2.1.21
68382leucine arylamidase-3.4.11.1

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119424-+++--------++-++---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
119424+-+/-++/-+--+++++-+-+++-+/-+++++++++++++++++/-++---+--+++/-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119424+++++-++++++++++++++++++-+-+---+++-++----+--------------+--++-----------------+-------+---++-------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling datelatitudelongitudeisolation date
8209alkaline groundwaterCabeco de VidePortugalPRTEurope
67772Non-saline alkaline groundwaterAlto-Alentejo, Cabeço de VidePortugalPRTEurope199939.1308-7.59
119424Environment, Alkaline ground waterCabeco de VidePortugalPRTEurope2000

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Groundwater
#Condition#Alkaline

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
82091Risk group (German classification)
677721
1194241Risk group (French classification)

Sequence information

16S sequences

  • @ref: 8209
  • description: Bacillus foraminis 16S rRNA gene, type strain CV53T
  • accession: AJ717382
  • length: 1534
  • database: nuccore
  • NCBI tax ID: 279826

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mesobacillus foraminis CV53GCA_013618915contigncbi279826
67772Mesobacillus foraminis CV53GCA_004340465scaffoldncbi279826

GC content

  • @ref: 8209
  • GC-content: 43.1

Genome-based predictions

predictions

@refmodeltraitdescriptionpredictionconfidencetraining_data
125438gram-positivegram-positivePositive reaction to Gram-stainingyes79.577no
125438anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no95.203no
125438aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes71.9yes
125438spore-formingspore-formingAbility to form endo- or exosporesyes92.283no
125438thermophilethermophilicAbility to grow at temperatures above or equal to 45°Cno91.814yes
125438motile2+flagellatedAbility to perform flagellated movementyes80.623no
125439BacteriaNetspore_formationAbility to form endo- or exosporesyes90.8
125439BacteriaNetmotilityAbility to perform movementyes85.5
125439BacteriaNetgram_stainReaction to gram-stainingvariable79.6
125439BacteriaNetoxygen_toleranceOxygenic conditions needed for growthfacultative anaerobe95.1

External links

@ref: 8209

culture collection no.: DSM 19613, CIP 108889, LMG 23174, UCCCB11

straininfo link

  • @ref: 70924
  • straininfo: 264971

literature

  • topic: Phylogeny
  • Pubmed-ID: 17082392
  • title: Bacillus foraminis sp. nov., isolated from a non-saline alkaline groundwater.
  • authors: Tiago I, Pires C, Mendes V, Morais PV, da Costa MS, Verissimo A
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.64281-0
  • year: 2006
  • mesh: Aerobiosis, Bacillus/*classification/cytology/*isolation & purification/physiology, Base Composition, Catalase/metabolism, Cell Wall/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis/chemistry, Genes, rRNA, Growth Inhibitors/pharmacology, Hydrogen-Ion Concentration, Molecular Sequence Data, Oxidoreductases/analysis/metabolism, Peptidoglycan/chemistry, Phylogeny, Quinones/analysis/chemistry, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Sodium Chloride/pharmacology, Temperature, *Water Microbiology
  • topic2: Enzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8209Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19613)https://www.dsmz.de/collection/catalogue/details/culture/DSM-19613
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
38357Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6568
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67772Curators of the UCCCBhttps://ucccb.uc.pt/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
70924Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID264971.1StrainInfo: A central database for resolving microbial strain identifiers
119424Curators of the CIPCollection of Institut Pasteur (CIP 108889)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108889
125438Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann10.1101/2024.08.12.607695Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets
125439Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardyhttps://github.com/GenomeNet/deepGdeepG: Deep Learning for Genome Sequence Data. R package version 0.3.1