Strain identifier

BacDive ID: 12672

Type strain: Yes

Species: Auraticoccus monumenti

Strain history: LMG 25551 <-- M. E. Trujillo MON 2.2 <-- P. Alonso-Vega.

NCBI tax ID(s): 675864 (species)

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General

@ref: 16762

BacDive-ID: 12672

DSM-Number: 23257

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, coccus-shaped

description: Auraticoccus monumenti DSM 23257 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from sandstone monument.

NCBI tax id

  • NCBI tax id: 675864
  • Matching level: species

strain history

@refhistory
16762<- M. E. Trujillo, Depart. Microbiol. y Gen., Univ. Salamanca, Spain; MON 2.2 <- P. Alonso-Vega
67770LMG 25551 <-- M. E. Trujillo MON 2.2 <-- P. Alonso-Vega.

doi: 10.13145/bacdive12672.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Propionibacteriales
  • family: Propionibacteriaceae
  • genus: Auraticoccus
  • species: Auraticoccus monumenti
  • full scientific name: Auraticoccus monumenti Alonso-Vega et al. 2011

@ref: 16762

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Propionibacteriaceae

genus: Auraticoccus

species: Auraticoccus monumenti

full scientific name: Auraticoccus monumenti Alonso-Vega et al. 2011

type strain: yes

Morphology

cell morphology

  • @ref: 29888
  • gram stain: positive
  • cell shape: coccus-shaped
  • motility: no

colony morphology

@refcolony colorincubation periodmedium used
21370Yellow orange (2000)10-14 daysISP 2
21370Pastel yellow (1034)10-14 daysISP 3
21370Dahlia yellow (1033)10-14 daysISP 4
21370Saffron yellow (1017)10-14 daysISP 5
21370Daffodil yellow (1007)10-14 daysISP 7
21370Daffodil yellow (1007)10-14 dayssuter with tyrosine
21370Daliah yellow (1033)10-14 dayssuter without tyrosine

multicellular morphology

@refforms multicellular complexmedium name
21370noISP 2
21370noISP 3
21370noISP 4
21370noISP 5
21370noISP 7
21370noSuter with tyrosine
21370noSuter without tyrosine

pigmentation

  • @ref: 29888
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16762MICROLUNATUS MEDIUM (DSMZ Medium 776)yeshttps://mediadive.dsmz.de/medium/776Name: MICROLUNATUS MEDIUM (DSMZ Medium 776) Composition: KH2PO4 0.5 g/l Na glutamate 0.5 g/l Yeast extract 0.5 g/l Peptone 0.5 g/l Glucose 0.5 g/l (NH4)2SO4 0.1 g/l MgSO4 x 7 H2O 0.1 g/l Distilled water
16762GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
21370ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
21370ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
21370ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
21370ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
21370ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
21370suter with tyrosineyesName: Synthetically suter medium (5337) with and without tyrosine - see Kutzner,H.J., R. M. Kroppensted and F. Korn-Wendisch. (1986) Composition: Glycerol 15.0 g/l Tyrosine 1.0 (optional, see preparation comment) L-arginine 5.0 g/l L-glutamic acid 5.0 g/l L-methionine 0.3 g/l L-isoleucine 0.3 g/l K2HPO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l Agar 20.0 g/l Trace element solution (5341) 1.0 ml Composition of Trace element solution (5341): CuSO4 x 5 H2O 10.0 g/l CaCl2 x 2 H2O 10.0 g/l FeSO4 x 7 H2O 10.0 g/l ZnSO4 x 7 H2O 10.0 g/l MnSO4 x 7 H2O 40.0 g/l Preparation: Control medium is prepared without tyrosine Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigment Organisms: All Actinomycetes
21370suter without tyrosineyesName: Synthetically suter medium (5337) with and without tyrosine - see Kutzner,H.J., R. M. Kroppensted and F. Korn-Wendisch. (1986) Composition: Glycerol 15.0 g/l Tyrosine 1.0 (optional, see preparation comment) L-arginine 5.0 g/l L-glutamic acid 5.0 g/l L-methionine 0.3 g/l L-isoleucine 0.3 g/l K2HPO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l Agar 20.0 g/l Trace element solution (5341) 1.0 ml Composition of Trace element solution (5341): CuSO4 x 5 H2O 10.0 g/l CaCl2 x 2 H2O 10.0 g/l FeSO4 x 7 H2O 10.0 g/l ZnSO4 x 7 H2O 10.0 g/l MnSO4 x 7 H2O 40.0 g/l Preparation: Control medium is prepared without tyrosine Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigment Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperaturerange
16762positivegrowth28mesophilic
29888positivegrowth15-30
29888positiveoptimum28mesophilic
67770positivegrowth28mesophilic

culture pH

@refabilitytypepHPH range
29888positivegrowth6.5-9alkaliphile
29888positiveoptimum7.75

Physiology and metabolism

oxygen tolerance

  • @ref: 29888
  • oxygen tolerance: aerobe

spore formation

  • @ref: 29888
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
21370NaClpositivemaximum5 %
29888NaClpositivegrowth01-05 %
29888NaClpositiveoptimum3 %

observation

@refobservation
29888aggregates in clumps
67770quinones: MK-8(H2), MK-9(H2), MK-9(H4), MK-9(H0)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2137017234glucose+
2137022599arabinose+
2137017992sucrose+
2137018222xylose+
2137017268myo-inositol+/-
2137037684mannose+
2137028757fructose+
2137026546rhamnose+
2137016634raffinose+
2137062968cellulose+
2988816449alanine+carbon source
2988829016arginine+carbon source
2988822653asparagine+carbon source
2988817057cellobiose+carbon source
2988828757fructose+carbon source
2988828260galactose+carbon source
298885291gelatin+carbon source
2988817234glucose+carbon source
2988817716lactose+carbon source
2988817306maltose+carbon source
2988829864mannitol+carbon source
2988837684mannose+carbon source
2988828053melibiose+carbon source
29888506227N-acetylglucosamine+carbon source
2988816634raffinose+carbon source
2988817814salicin+carbon source
2988817822serine+carbon source
2988817992sucrose+carbon source
2988827082trehalose+carbon source
2988818222xylose+carbon source
298884853esculin+hydrolysis
6837917632nitrate-reduction
683794853esculin+hydrolysis
6837916199urea-hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose+fermentation
6837916988D-ribose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation

enzymes

@refvalueactivityec
29888acid phosphatase+3.1.3.2
29888alkaline phosphatase+3.1.3.1
29888alpha-galactosidase+3.2.1.22
29888catalase+1.11.1.6
29888gelatinase+
29888cytochrome oxidase+1.9.3.1
68379gelatinase+
68379urease-3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase+3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase+3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
21370-+/-++-++++-++-+/-----+/-

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
21370+++-++++/-+/-++++-+++++

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
16762sandstone monumentSalamancaSpainESPEurope
67770Deteriorated sandstone monumentSalamancaSpainESPEurope

isolation source categories

Cat1Cat2Cat3
#Engineered#Built environment
#Environmental#Terrestrial#Geologic

taxonmaps

  • @ref: 69479
  • File name: preview.99_116551.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_72;96_22632;97_28291;98_36041;99_116551&stattab=map
  • Last taxonomy: Auraticoccus monumenti subclade
  • 16S sequence: FN552748
  • Sequence Identity:
  • Total samples: 2981
  • soil counts: 1007
  • aquatic counts: 529
  • animal counts: 1204
  • plant counts: 241

Safety information

risk assessment

  • @ref: 16762
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 16762
  • description: Auraticoccus monumenti partial 16S rRNA gene, type strain MON 2.2T
  • accession: FN552748
  • length: 1449
  • database: ena
  • NCBI tax ID: 675864

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Auraticoccus monumenti strain MON 2.2675864.4completepatric675864
66792Auraticoccus monumenti DSM 232572636415982draftimg675864
67770Auraticoccus monumenti MON 2.2GCA_900101785chromosomencbi675864

GC content

@refGC-contentmethod
1676273.5thermal denaturation, midpoint method (Tm)
2988873.5

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
gram-positiveyes89.594yes
anaerobicno99.706yes
halophileno90.717no
spore-formingno80.955no
glucose-utilyes85.294yes
thermophileno99.364yes
flagellatedno98.642yes
motileno93.663yes
aerobicyes94.312yes
glucose-fermentno82.67yes

External links

@ref: 16762

culture collection no.: DSM 23257, CECT 7672, LMG 25551, MON 2.2, JCM 18954

straininfo link

  • @ref: 81883
  • straininfo: 368627

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny20525813Auraticoccus monumenti gen. nov., sp. nov., an actinomycete isolated from a deteriorated sandstone monument.Alonso-Vega P, Carro L, Martinez-Molina E, Trujillo MEInt J Syst Evol Microbiol10.1099/ijs.0.024257-02010Actinobacteria/*classification/genetics/*isolation & purification/metabolism, DNA, Bacterial/genetics, *Environmental Microbiology, Fatty Acids/metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/geneticsMetabolism
Phylogeny32302275Auraticoccus cholistanensis sp. nov., an actinomycete isolated from soil of the Cholistan Desert, and emended description of the genus Auraticoccus.Cheema MT, Ye JJ, Li FN, Lu QP, Abbas M, Sajid I, Huang DL, Liu SW, Sun CHInt J Syst Evol Microbiol10.1099/ijsem.0.0041522020Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, *Desert Climate, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Glycolipids/chemistry, Nucleic Acid Hybridization, Pakistan, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, Propionibacteriaceae/*classification/isolation & purification, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmedjournal
16762Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23257)https://www.dsmz.de/collection/catalogue/details/culture/DSM-23257
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
21370Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM23257.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
29888Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2626128776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updates33211880Nucleic Acids Res. 49: D498-D508 2020
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
81883Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID368627.1StrainInfo: A central database for resolving microbial strain identifiers