Strain identifier
BacDive ID: 12653
Type strain:
Species: Arachnia propionica
Strain Designation: 699
Strain history: CIP <- 1985, ATCC <- L. Pine: strain 699, Actinomyces propionicus <- S.S. Roberts
NCBI tax ID(s): 1218109 (strain), 1750 (species)
General
@ref: 10945
BacDive-ID: 12653
DSM-Number: 43307
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic
description: Arachnia propionica 699 is an anaerobe, mesophilic bacterium that was isolated from lacrimal canaliculitis.
NCBI tax id
NCBI tax id | Matching level |
---|---|
1750 | species |
1218109 | strain |
strain history
@ref | history |
---|---|
10945 | <- P. Hirsch <- ATCC <- L. Pine, 699 |
67770 | ATCC 14157 <-- L. Pine 699. |
121965 | CIP <- 1985, ATCC <- L. Pine: strain 699, Actinomyces propionicus <- S.S. Roberts |
doi: 10.13145/bacdive12653.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Propionibacteriales
- family: Propionibacteriaceae
- genus: Arachnia
- species: Arachnia propionica
- full scientific name: Arachnia propionica (Buchanan and Pine 1962) Pine and Georg 1969 (Approved Lists 1980)
synonyms
@ref synonym 20215 Actinomyces propionicus 20215 Propionibacterium propionicus 20215 Pseudopropionibacterium propionicum 20215 Propionibacterium propionicum
@ref: 10945
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Propionibacteriaceae
genus: Arachnia
species: Arachnia propionica
full scientific name: Arachnia propionica (Buchanan and Pine 1962) Pine and Georg 1969
strain designation: 699
type strain: yes
Morphology
cell morphology
@ref | motility | confidence | gram stain | cell shape |
---|---|---|---|---|
69480 | no | 94.98 | ||
69480 | 100 | positive | ||
121965 | no | positive | rod-shaped |
colony morphology
- @ref: 121965
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
10945 | BHI/1 MEDIUM (DSMZ Medium 216) | yes | https://mediadive.dsmz.de/medium/216 | Name: BHI/1 MEDIUM (DSMZ Medium 216) Composition: Brain heart infusion 37.0 g/l Yeast extract 2.0 g/l Distilled water |
42209 | MEDIUM 20 - for Anaerobic bacteria | yes | Agar (15.000 g);Glucose (5.000 g);Yeast extract (20.000 g);Tryptone (30.000 g);Cysteine hydrochloride (0.500 g);distilled water (1000.000 ml);Hemin solution -M00149 (25.000 ml) | |
121965 | CIP Medium 66 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=66 | |
121965 | CIP Medium 20 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=20 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
10945 | positive | growth | 37 | mesophilic |
42209 | positive | growth | 37 | mesophilic |
44809 | positive | growth | 37 | mesophilic |
67770 | positive | growth | 37 | mesophilic |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
10945 | anaerobe |
44809 | anaerobe |
44809 | microaerophile |
121965 | facultative anaerobe |
spore formation
- @ref: 69480
- spore formation: no
- confidence: 99.868
murein
- @ref: 10945
- murein short key: A42.01
- type: A3gamma' LL-Dpm-Gly
metabolite utilization
@ref | metabolite | utilization activity | kind of utilization tested | Chebi-ID |
---|---|---|---|---|
68371 | Potassium 2-ketogluconate | - | builds acid from | |
68371 | gluconate | - | builds acid from | 24265 |
68371 | L-arabitol | - | builds acid from | 18403 |
68371 | D-arabitol | + | builds acid from | 18333 |
68371 | L-fucose | + | builds acid from | 18287 |
68371 | D-fucose | + | builds acid from | 28847 |
68371 | D-tagatose | - | builds acid from | 16443 |
68371 | D-lyxose | - | builds acid from | 62318 |
68371 | gentiobiose | - | builds acid from | 28066 |
68371 | xylitol | - | builds acid from | 17151 |
68371 | glycogen | - | builds acid from | 28087 |
68371 | starch | - | builds acid from | 28017 |
68371 | raffinose | + | builds acid from | 16634 |
68371 | melezitose | - | builds acid from | 6731 |
68371 | inulin | - | builds acid from | 15443 |
68371 | sucrose | + | builds acid from | 17992 |
68371 | lactose | + | builds acid from | 17716 |
68371 | maltose | + | builds acid from | 17306 |
68371 | cellobiose | - | builds acid from | 17057 |
68371 | salicin | - | builds acid from | 17814 |
68371 | esculin | - | builds acid from | 4853 |
68371 | arbutin | - | builds acid from | 18305 |
68371 | amygdalin | - | builds acid from | 27613 |
68371 | N-acetylglucosamine | - | builds acid from | 59640 |
68371 | methyl alpha-D-glucopyranoside | - | builds acid from | 320061 |
68371 | methyl alpha-D-mannoside | - | builds acid from | 43943 |
68371 | D-sorbitol | - | builds acid from | 17924 |
68371 | myo-inositol | - | builds acid from | 17268 |
68371 | galactitol | - | builds acid from | 16813 |
68371 | L-rhamnose | - | builds acid from | 62345 |
68371 | L-sorbose | - | builds acid from | 17266 |
68371 | D-fructose | + | builds acid from | 15824 |
68371 | D-glucose | + | builds acid from | 17634 |
68371 | D-galactose | - | builds acid from | 12936 |
68371 | methyl beta-D-xylopyranoside | - | builds acid from | 74863 |
68371 | ribitol | - | builds acid from | 15963 |
68371 | L-xylose | - | builds acid from | 65328 |
68371 | D-xylose | - | builds acid from | 65327 |
68371 | D-ribose | - | builds acid from | 16988 |
68371 | L-arabinose | - | builds acid from | 30849 |
68371 | D-arabinose | - | builds acid from | 17108 |
68371 | erythritol | - | builds acid from | 17113 |
68371 | glycerol | - | builds acid from | 17754 |
121965 | D-arabinose | + | degradation | 17108 |
121965 | D-fructose | - | degradation | 15824 |
121965 | D-glucose | - | degradation | 17634 |
121965 | D-xylose | + | degradation | 65327 |
121965 | cellobiose | - | degradation | 17057 |
121965 | lactose | - | degradation | 17716 |
121965 | maltose | + | degradation | 17306 |
121965 | salicin | - | degradation | 17814 |
121965 | sucrose | - | degradation | 17992 |
121965 | esculin | - | hydrolysis | 4853 |
121965 | nitrate | + | reduction | 17632 |
121965 | nitrite | - | reduction | 16301 |
121965 | nitrate | + | respiration | 17632 |
68380 | urea | - | hydrolysis | 16199 |
68380 | arginine | - | hydrolysis | 29016 |
68380 | D-mannose | - | fermentation | 16024 |
68380 | raffinose | - | fermentation | 16634 |
68380 | L-glutamate | - | degradation | 29985 |
68380 | nitrate | - | reduction | 17632 |
68380 | tryptophan | - | energy source | 27897 |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
121965 | 35581 | indole | no |
68380 | 35581 | indole | no |
metabolite tests
- @ref: 68380
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
68380 | serine arylamidase | + | |
68380 | glutamyl-glutamate arylamidase | - | |
68380 | alanine arylamidase | + | 3.4.11.2 |
68380 | tyrosine arylamidase | + | |
68380 | pyrrolidonyl arylamidase | - | 3.4.19.3 |
68380 | leucine arylamidase | + | 3.4.11.1 |
68380 | phenylalanine arylamidase | + | |
68380 | leucyl glycin arylamidase | - | 3.4.11.1 |
68380 | L-arginine arylamidase | + | |
68380 | alkaline phosphatase | - | 3.1.3.1 |
68380 | tryptophan deaminase | - | 4.1.99.1 |
68380 | glutamate decarboxylase | - | 4.1.1.15 |
68380 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68380 | beta-glucuronidase | - | 3.2.1.31 |
68380 | alpha-arabinosidase | - | 3.2.1.55 |
68380 | beta-glucosidase | - | 3.2.1.21 |
68380 | alpha-glucosidase | + | 3.2.1.20 |
68380 | beta-Galactosidase 6-phosphate | - | |
68380 | beta-galactosidase | + | 3.2.1.23 |
68380 | alpha-galactosidase | + | 3.2.1.22 |
68380 | arginine dihydrolase | - | 3.5.3.6 |
68380 | urease | - | 3.5.1.5 |
121965 | oxidase | - | |
121965 | beta-galactosidase | - | 3.2.1.23 |
121965 | gelatinase | - | |
121965 | amylase | + | |
121965 | DNase | - | |
121965 | caseinase | - | 3.4.21.50 |
121965 | catalase | - | 1.11.1.6 |
121965 | tween esterase | - | |
121965 | lecithinase | - | |
121965 | lipase | - | |
121965 | protease | - | |
121965 | urease | - | 3.5.1.5 |
68382 | alkaline phosphatase | - | 3.1.3.1 |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | + | |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | - | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | - | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | alpha-galactosidase | + | 3.2.1.22 |
68382 | beta-galactosidase | + | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | + | 3.2.1.20 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | + | 3.2.1.51 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
121965 | - | - | + | + | - | + | - | - | - | - | + | + | + | + | - | + | - | - | - | + |
API 50CHac
@ref | GLY | ERY | DARA | LARA | RIB | DXYL | LXYL | ADO | MDX | GAL | GLU | FRU | MNE | SBE | RHA | DUL | INO | MAN | SOR | MDM | MDG | NAG | AMY | ARB | ESC | SAL | CEL | MAL | LAC | MEL | SAC | TRE | INU | MLZ | RAF | AMD | GLYG | XLT | GEN | TUR | LYX | TAG | DFUC | LFUC | DARL | LARL | GNT | 2KG | 5KG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
121965 | - | - | - | - | - | - | - | - | - | - | + | + | +/- | - | - | - | - | +/- | - | - | - | - | - | - | - | - | - | + | + | +/- | + | +/- | - | - | + | - | - | - | - | +/- | - | - | + | + | + | - | - | - | +/- |
API rID32A
@ref | URE | ADH Arg | alpha GAL | beta GAL | beta GP | alpha GLU | beta GLU | alpha ARA | beta GUR | beta NAG | MNE | RAF | GDC | alpha FUC | NIT | IND | PAL | ArgA | ProA | LGA | PheA | LeuA | PyrA | TyrA | AlaA | GlyA | HisA | GGA | SerA |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
10945 | - | - | + | + | - | + | - | - | - | - | - | - | - | +/- | - | - | - | + | +/- | - | + | + | - | + | + | +/- | +/- | - | + |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
10945 | lacrimal canaliculitis | ||||
44809 | Human lacrimal duct,small concretions | NC,Durham | USA | USA | North America |
67770 | Lacrimal canaliculitis | ||||
121965 | Human, Small concretions from human lacrimal duct | Durham | United Kingdom | GBR | Europe |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Infection | #Disease | |
#Host Body-Site | #Organ | #Eye |
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
10945 | 2 | Risk group (German classification) |
121965 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Propionibacterium propionicum strain CCUG 4939 clone 1 16S-23S ribosomal RNA intergenic spacer, partial sequence | GQ496478 | 315 | ena | 1750 |
20218 | Propionibacterium propionicus 16S ribosomal RNA gene, partial sequence | AF285117 | 1447 | ena | 1750 |
20218 | Propionibacterium propionicus DSM 43307 16S rRNA gene | AJ003058 | 1506 | ena | 1750 |
20218 | Propionibacterium propionicus 16S rRNA gene, strain DSM 43307T | AJ315953 | 1503 | ena | 1750 |
20218 | Propionibacterium propionicus partial 16S rRNA | X53216 | 1179 | ena | 1750 |
10945 | Pseudopropionibacterium propionicum strain DSM 43307 16S ribosomal RNA, partial sequence | NR_114803 | 1503 | nuccore | 1750 |
67770 | Propionibacterium propionicum gene for 16S ribosomal RNA, partial sequence, strain: JCM 5830 | AB971802 | 1488 | ena | 1750 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Arachnia propionica NCTC12967 | GCA_900637725 | complete | ncbi | 1750 |
66792 | Propionibacterium propionicum NBRC 14587 | 1218109.3 | wgs | patric | 1218109 |
66792 | Pseudopropionibacterium propionicum strain NCTC12967 | 1750.7 | complete | patric | 1750 |
66792 | Pseudopropionibacterium propionicum NBRC 14587 | 2731957689 | draft | img | 1218109 |
67770 | Arachnia propionica NBRC 14587 | GCA_001592325 | contig | ncbi | 1218109 |
GC content
- @ref: 67770
- GC-content: 65
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 89 | no |
flagellated | no | 98.03 | no |
gram-positive | yes | 90.779 | no |
anaerobic | no | 80.988 | no |
aerobic | no | 77.004 | no |
halophile | no | 91.798 | no |
spore-forming | no | 87.513 | no |
thermophile | no | 96.795 | yes |
glucose-util | yes | 85.845 | no |
motile | no | 93.354 | no |
glucose-ferment | yes | 73.934 | no |
External links
@ref: 10945
culture collection no.: DSM 43307, ATCC 14157, CIP 110010, CCUG 4939, LMG 16729, ATCC 13682, JCM 5830, CIP 101941, IFO 14587, IMET 10974, LMG 19873, NBRC 14587, NCTC 12967, PCM 2431, VKM Ac-1449, VPI 0026, NBRC 4587
straininfo link
- @ref: 81866
- straininfo: 13971
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Metabolism | 12427753 | Novel bacterial polar lipids containing ether-linked alkyl chains, the structures and biological properties of the four major glycolipids from Propionibacterium propionicum PCM 2431 (ATCC 14157T). | Pasciak M, Holst O, Lindner B, Mordarska H, Gamian A | J Biol Chem | 10.1074/jbc.M206013200 | 2002 | Carbohydrate Sequence, Chromatography, Thin Layer, Ethers/*chemistry, Gas Chromatography-Mass Spectrometry, Glycolipids/chemistry/*metabolism, Magnetic Resonance Spectroscopy, Methylation, Molecular Structure, Propionibacterium/*metabolism, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization | |
Phylogeny | 16400987 | [Isolation and comparative analysis of glycolipid fractions in bifidobacteria]. | Novik GI, Astapovich NI, Grzegorzewicz A, Gamian A | Mikrobiologiia | 2005 | Bifidobacterium/*chemistry, Chromatography, Thin Layer, Glycolipids/*analysis/isolation & purification | Enzymology | |
Phylogeny | 20971831 | Propioniciclava tarda gen. nov., sp. nov., isolated from a methanogenic reactor treating waste from cattle farms. | Sugawara Y, Ueki A, Abe K, Kaku N, Watanabe K, Ueki K | Int J Syst Evol Microbiol | 10.1099/ijs.0.027482-0 | 2010 | Aerobiosis, Anaerobiosis, Animals, Bacterial Typing Techniques, Base Composition, Bioreactors/*microbiology, Carbohydrate Metabolism, Catalase/metabolism, Cattle, Cell Wall/chemistry, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Hydrogen-Ion Concentration, *Industrial Waste, Japan, Molecular Sequence Data, Nitrates/metabolism, Oxidation-Reduction, Phylogeny, Propionibacteriaceae/*classification/genetics/*isolation & purification/physiology, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Temperature, Vitamin B 12/metabolism | Metabolism |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
10945 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43307) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-43307 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
42209 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/13572 | ||||
44809 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 4939) | https://www.ccug.se/strain?id=4939 | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68371 | Automatically annotated from API 50CH acid | |||||
68380 | Automatically annotated from API rID32A | |||||
68382 | Automatically annotated from API zym | |||||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
81866 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID13971.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
121965 | Curators of the CIP | Collection of Institut Pasteur (CIP 101941) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20101941 |