Strain identifier

BacDive ID: 12651

Type strain: Yes

Species: Acidipropionibacterium jensenii

Strain Designation: 29

Strain history: CIP <- 1987, NCIB <- 1950, ATCC <- C.B. van Niel: strain 29

NCBI tax ID(s): 1122997 (strain), 1749 (species)

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General

@ref: 8765

BacDive-ID: 12651

DSM-Number: 20535

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, oval-shaped

description: Acidipropionibacterium jensenii 29 is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from buttermilk.

NCBI tax id

NCBI tax idMatching level
1749species
1122997strain

strain history

@refhistory
8765<- ATCC <- C.B. van Niel, 29
120337CIP <- 1987, NCIB <- 1950, ATCC <- C.B. van Niel: strain 29

doi: 10.13145/bacdive12651.20250331.9.3

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Propionibacteriales
  • family: Propionibacteriaceae
  • genus: Acidipropionibacterium
  • species: Acidipropionibacterium jensenii
  • full scientific name: Acidipropionibacterium jensenii (van Niel 1928) Scholz and Kilian 2016

  • synonyms

    • @ref: 20215
    • synonym: Propionibacterium jensenii

@ref: 8765

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Propionibacteriaceae

genus: Acidipropionibacterium

species: Acidipropionibacterium jensenii

full scientific name: Acidipropionibacterium jensenii (van Niel 1928) Scholz and Kilian 2016 emend. Nouioui et al. 2018

strain designation: 29

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
120337positiveoval-shapedno
125439positive99.3
125438no94

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8765PROPIONIBACTERIUM AGAR (DSMZ Medium 91)yeshttps://mediadive.dsmz.de/medium/91Name: PROPIONIBACTERIUM AGAR (DSMZ Medium 91) Composition: Agar 15.0 g/l Na-lactate 10.0 g/l Casein peptone 10.0 g/l Yeast extract 5.0 g/l Distilled water
42201MEDIUM 20 - for Anaerobic bacteriayesAgar (15.000 g);Glucose (5.000 g);Yeast extract (20.000 g);Tryptone (30.000 g);Cysteine hydrochloride (0.500 g);distilled water (1000.000 ml);Hemin solution -M00149 (25.000 ml)
120337CIP Medium 20yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=20

culture temp

@refgrowthtypetemperature
8765positivegrowth30
42201positivegrowth37
58380positivegrowth30

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
8765anaerobe
58380anaerobe
120337facultative anaerobe

murein

  • @ref: 8765
  • murein short key: A41.01
  • type: A3gamma LL-Dpm-Gly

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838029985L-glutamate-degradation
6838016634raffinose+fermentation
6838029016arginine-hydrolysis
6838016024D-mannose+fermentation
6838016199urea-hydrolysis
12033717632nitrate-reduction
12033716301nitrite-reduction
68371Potassium 5-ketogluconate-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6838027897tryptophan-energy source
6838017632nitrate-reduction
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol+builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose-builds acid from
6837128053melibiose+builds acid from
6837117716lactose+builds acid from
6837117306maltose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol+builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose+builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol+builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose+builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol+builds acid from

metabolite production

@refChebi-IDmetaboliteproduction
6838035581indoleno
12033735581indoleno

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase+3.2.1.31
68382beta-galactosidase+3.2.1.23
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382alkaline phosphatase-3.1.3.1
120337oxidase-
120337catalase-1.11.1.6
120337urease-3.5.1.5
68380glycin arylamidase+
68380tyrosine arylamidase+
68380leucine arylamidase+3.4.11.1
68380phenylalanine arylamidase+
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase-3.2.1.23
68382alpha-galactosidase+3.2.1.22
68380serine arylamidase+
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase+
68380alanine arylamidase+3.4.11.2
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase+3.4.11.5
68380L-arginine arylamidase+
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380alpha-arabinosidase-3.2.1.55
68380alpha-galactosidase+3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    58380C13:00.613
    58380C14:00.814
    58380C15:020.815
    58380C16:0316
    58380C17:03.217
    58380C18:01.118
    58380C13:0 ANTEISO0.312.701
    58380C13:0 iso0.412.612
    58380C14:0 ISO0.813.618
    58380C15:0 ANTEISO23.114.711
    58380C15:0 ISO24.514.621
    58380C16:0 iso0.415.626
    58380C16:1 ISO H4.915.461
    58380C16:1 ω7c0.315.819
    58380C17:0 anteiso0.516.722
    58380C17:0 iso2.416.629
    58380C17:1 ω5c ISO0.916.461
    58380C18:1 ω9c2.317.769
    58380C18:2 ω6,9c/C18:0 ANTE4.617.724
    58380Unidentified0.6
    58380Unidentified1.1
    58380Unidentified2.7
    58380Unidentified0.5
    58380unknown 17.1050.317.105
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120337-----+----+++++-++--

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
120337+---+--+-++++---+/-+------+/----++--------------+----

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
8765--+--+/-+/----++-----++-++-++++-+

Isolation, sampling and environmental information

isolation

@refsample type
8765buttermilk
58380Buttermilk
120337Food, Buttermilk

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Food production
  • Cat3: #Dairy product

taxonmaps

  • @ref: 69479
  • File name: preview.99_5608.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15360;96_2703;97_3321;98_4184;99_5608&stattab=map
  • Last taxonomy: Propionibacteriaceae
  • 16S sequence: AJ704571
  • Sequence Identity:
  • Total samples: 3135
  • soil counts: 179
  • aquatic counts: 788
  • animal counts: 2096
  • plant counts: 72

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
8765yes, in single cases1Risk group (German classification)
1203371Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Propionibacterium jensenii partial 16S rRNA gene, type strain CIP 103028TAM412523182nuccore1749
20218Propionibacterium jensenii strain CIP 103028 16S ribosomal RNA gene, partial sequenceAY883044461nuccore1749
20218Propionibacterium jensenii partial 16S rRNA gene, type strain DSM 20535AJ7045711487nuccore1749
20218Propionibacterium jensenii partial 16S rRNAX532191364nuccore1749

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Acidipropionibacterium jensenii DSM 20535GCA_000425285scaffoldncbi1122997
66792Propionibacterium jensenii DSM 205351122997.3wgspatric1122997
66792Acidipropionibacterium jensenii DSM 205352523533551draftimg1122997

GC content

  • @ref: 8765
  • GC-content: 66.0

Genome-based predictions

predictions

@refmodeltraitdescriptionpredictionconfidencetraining_data
125438gram-positivegram-positivePositive reaction to Gram-stainingyes88.053no
125438anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no76.308no
125438aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)no58.514no
125438spore-formingspore-formingAbility to form endo- or exosporesno79.532no
125438thermophilethermophilicAbility to grow at temperatures above or equal to 45°Cno94.5yes
125438motile2+flagellatedAbility to perform flagellated movementno94no
125439BacteriaNetspore_formationAbility to form endo- or exosporesno54.9
125439BacteriaNetmotilityAbility to perform movementno77.3
125439BacteriaNetgram_stainReaction to gram-stainingpositive99.3
125439BacteriaNetoxygen_toleranceOxygenic conditions needed for growthfacultative anaerobe80.1

External links

@ref: 8765

culture collection no.: CCUG 48883, CIP 103028, NCIMB 8071, DSM 20535, ATCC 4868, NCDO 850, NCIB 8071

straininfo link

  • @ref: 81864
  • straininfo: 139519

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism23709505Development of a Propionibacterium-Escherichia coli shuttle vector for metabolic engineering of Propionibacterium jensenii, an efficient producer of propionic acid.Zhuge X, Liu L, Shin HD, Chen RR, Li J, Du G, Chen JAppl Environ Microbiol10.1128/AEM.00737-132013Cloning, Molecular, Escherichia coli/*genetics/metabolism, Genetic Vectors/genetics/metabolism, Metabolic Engineering, Molecular Sequence Data, Plasmids/genetics/metabolism, Propionates/*metabolism, Propionibacterium/*genetics/metabolism, Reverse Transcriptase Polymerase Chain Reaction, Sequence Analysis, DNA, Sequence Analysis, ProteinTranscriptome
Metabolism25453933Improved propionic acid production with metabolically engineered Propionibacterium jensenii by an oxidoreduction potential-shift control strategy.Zhuge X, Li J, Shin HD, Liu L, Du G, Chen JBioresour Technol10.1016/j.biortech.2014.10.0382014Batch Cell Culture Techniques/instrumentation, Bioreactors/microbiology, Industrial Microbiology/instrumentation/*methods, Metabolic Engineering/*methods, NAD/metabolism, Oxidation-Reduction, Propionates/*metabolism, Propionibacterium/genetics/*metabolismBiotechnology
Biotechnology26666200Metabolic engineering of acid resistance elements to improve acid resistance and propionic acid production of Propionibacterium jensenii.Guan N, Li J, Shin HD, Du G, Chen J, Liu LBiotechnol Bioeng10.1002/bit.259022015Cell Proliferation/physiology, Genetic Enhancement/methods, Glutamate Decarboxylase/*genetics, Hydrogen-Ion Concentration, Hydrolases/*genetics, Metabolic Engineering/*methods, Propionates/isolation & purification/*metabolism, Propionibacterium/*chemistry/*physiologyEnzymology
Metabolism30497590A low-power ultrasound attenuation improves the stability of biofilm and hydrophobicity of Propionibacterium freudenreichii subsp. freudenreichii DSM 20271 and Acidipropionibacterium jensenii DSM 20535.Bevilacqua A, Racioppo A, Sinigaglia M, Speranza B, Campaniello D, Corbo MRFood Microbiol10.1016/j.fm.2018.10.0102018Acids/metabolism, Biofilms/*growth & development, Hydrogen-Ion Concentration, *Hydrophobic and Hydrophilic Interactions, Microbial Viability, Permeability, Propionibacterium/*physiology, Propionibacterium freudenreichii/growth & development/*physiology, Sonication, *Ultrasonics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8765Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20535)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20535
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
42201Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14780
58380Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 48883)https://www.ccug.se/strain?id=48883
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68371Automatically annotated from API 50CH acid
68380Automatically annotated from API rID32A
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
81864Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID139519.1StrainInfo: A central database for resolving microbial strain identifiers
120337Curators of the CIPCollection of Institut Pasteur (CIP 103028)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103028
125438Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann10.1101/2024.08.12.607695Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets
125439Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardyhttps://github.com/GenomeNet/deepGdeepG: Deep Learning for Genome Sequence Data. R package version 0.3.1