Strain identifier

BacDive ID: 12634

Type strain: No

Species: Acidipropionibacterium acidipropionici

Strain Designation: 4

Strain history: CIP <- 1953, ATCC, Bacterium acidi-propionici <- C.B. van Niel, Hopkins Marine Stat.: strain 4, Propionibacterium pentosaceum

NCBI tax ID(s): 1171373 (strain), 1748 (species)

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General

@ref: 8679

BacDive-ID: 12634

DSM-Number: 20272

keywords: genome sequence, 16S sequence, Bacteria, obligate anaerobe, mesophilic, Gram-positive, rod-shaped

description: Acidipropionibacterium acidipropionici 4 is an obligate anaerobe, mesophilic, Gram-positive bacterium that was isolated from emmental cheese.

NCBI tax id

NCBI tax idMatching level
1171373strain
1748species

strain history

@refhistory
8679<- Weihenstephan, Germany <- ATCC <- C.B. van Niel, 4 (Propionibacterium pentosaceum)
67770T. Mitsuoka <-- ATCC 4875 <-- C. B. van Niel 4.
116014CIP <- 1953, ATCC, Bacterium acidi-propionici <- C.B. van Niel, Hopkins Marine Stat.: strain 4, Propionibacterium pentosaceum

doi: 10.13145/bacdive12634.20250331.9.3

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Propionibacteriales
  • family: Propionibacteriaceae
  • genus: Acidipropionibacterium
  • species: Acidipropionibacterium acidipropionici
  • full scientific name: Acidipropionibacterium acidipropionici (Orla-Jensen 1909) Scholz and Kilian 2016

  • synonyms

    • @ref: 20215
    • synonym: Propionibacterium acidipropionici

@ref: 8679

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Propionibacteriaceae

genus: Acidipropionibacterium

species: Acidipropionibacterium acidipropionici

full scientific name: Acidipropionibacterium acidipropionici (Orla-Jensen 1909) Scholz and Kilian 2016 emend. Nouioui et al. 2018

strain designation: 4

type strain: no

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
116014positiverod-shapedno
125438no92
125439no96.1
125439positive99.9

colony morphology

  • @ref: 116014

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8679PYG MEDIUM (MODIFIED) (DSMZ Medium 104)yeshttps://mediadive.dsmz.de/medium/104Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water
8679PROPIONIBACTERIUM AGAR (DSMZ Medium 91)yeshttps://mediadive.dsmz.de/medium/91Name: PROPIONIBACTERIUM AGAR (DSMZ Medium 91) Composition: Agar 15.0 g/l Na-lactate 10.0 g/l Casein peptone 10.0 g/l Yeast extract 5.0 g/l Distilled water
42208MEDIUM 20 - for Anaerobic bacteriayesAgar (15.000 g);Glucose (5.000 g);Yeast extract (20.000 g);Tryptone (30.000 g);Cysteine hydrochloride (0.500 g);distilled water (1000.000 ml);Hemin solution -M00149 (25.000 ml)
116014CIP Medium 20yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=20

culture temp

@refgrowthtypetemperature
8679positivegrowth30
42208positivegrowth30
67770positivegrowth30

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
8679obligate anaerobe
116014facultative anaerobe
125439facultative anaerobe96.4

murein

  • @ref: 8679
  • murein short key: A41.01
  • type: A3gamma LL-Dpm-Gly

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838027897tryptophan-energy source
6838016634raffinose+fermentation
6838029016arginine-hydrolysis
6838016024D-mannose+fermentation
1160144853esculin+hydrolysis
11601417632nitrate+reduction
11601416301nitrite-reduction
11601435020tributyrin+hydrolysis
11601417632nitrate+respiration
68371Potassium 5-ketogluconate-builds acid from
683714853esculin+builds acid from
6837117754glycerol+builds acid from
6838017632nitrate-reduction
6838029985L-glutamate-degradation
6838016199urea-hydrolysis
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate+builds acid from
6837118403L-arabitol+builds acid from
6837118333D-arabitol+builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose+builds acid from
6837116634raffinose+builds acid from
6837115443inulin-builds acid from
6837117992sucrose+builds acid from
6837128053melibiose+builds acid from
6837117716lactose+builds acid from
6837117306maltose+builds acid from
6837159640N-acetylglucosamine+builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol+builds acid from
6837116899D-mannitol+builds acid from
6837117268myo-inositol+builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose+builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose+builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol+builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose+builds acid from
6837116988D-ribose+builds acid from
6837130849L-arabinose+builds acid from
6837117108D-arabinose+builds acid from
6837117113erythritol+builds acid from

metabolite production

@refChebi-IDmetaboliteproduction
6838035581indoleno
11601435581indoleno

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382alkaline phosphatase+3.1.3.1
116014oxidase-
116014beta-galactosidase+3.2.1.23
116014gelatinase-
116014amylase+
116014DNase-
116014caseinase-3.4.21.50
116014catalase-1.11.1.6
116014tween esterase+
116014lecithinase-
116014lipase-
116014protease-
116014urease-3.5.1.5
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucyl glycin arylamidase-3.4.11.1
68380L-arginine arylamidase+
68380alkaline phosphatase+3.1.3.1
68380glutamate decarboxylase-4.1.1.15
68380urease-3.5.1.5
68382alpha-galactosidase+3.2.1.22
68380glutamyl-glutamate arylamidase-
68380proline-arylamidase+3.4.11.5
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase+3.2.1.55
68380alpha-glucosidase+3.2.1.20
68380beta-galactosidase+3.2.1.23
68380alpha-galactosidase+3.2.1.22
68380arginine dihydrolase-3.5.3.6

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116014-+---+-+--++++-+++--

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
116014++++++-+-++++-+-+++-+/-++/-+/-++/-+/-+++++/--+/-++/-+/-+/-+/-+----+++--

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
8679--++-+++--++----+++-+/-+/--++++-+
8679--++++++--++----+++-++-++/-+/-+-+
8679--++-++/-+--++----+++----------

Isolation, sampling and environmental information

isolation

@refsample type
8679emmental cheese
67770Emmenthaler cheese
116014Food, Cheese, Emmenthal

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Food production
  • Cat3: #Dairy product

taxonmaps

  • @ref: 69479
  • File name: preview.99_3995.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15360;96_2030;97_2446;98_3026;99_3995&stattab=map
  • Last taxonomy: Acidipropionibacterium acidipropionici
  • 16S sequence: AB536774
  • Sequence Identity:
  • Total samples: 22874
  • soil counts: 3020
  • aquatic counts: 3912
  • animal counts: 15402
  • plant counts: 540

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
86791Risk group (German classification)
1160141Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Propionibacterium acidi-propionici partial 16S rRNAX532211351nuccore1171373
67770Propionibacterium acidipropionici gene for 16S ribosomal RNA, partial sequence, strain: JCM 6432AB5367741495nuccore1171373

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Acidipropionibacterium acidipropionici ATCC 4875GCA_000310065completencbi1171373
66792Propionibacterium acidipropionici ATCC 48751171373.8completepatric1171373
66792Acidipropionibacterium acidipropionici ATCC 48752521172602completeimg1171373
66792Acidipropionibacterium acidipropionici ATCC 48752518285597draftimg1171373

GC content

@refGC-contentmethod
867966.0
6777066thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

@refmodeltraitdescriptionpredictionconfidencetraining_data
125439BacteriaNetoxygen_toleranceOxygenic conditions needed for growthfacultative anaerobe96.4
125439BacteriaNetgram_stainReaction to gram-stainingpositive99.9
125439BacteriaNetmotilityAbility to perform movementno96.1
125439BacteriaNetspore_formationAbility to form endo- or exosporesyes50.9
125438gram-positivegram-positivePositive reaction to Gram-stainingyes89.237no
125438anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no74.999no
125438aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)no63.671no
125438spore-formingspore-formingAbility to form endo- or exosporesno72.547no
125438thermophilethermophilicAbility to grow at temperatures above or equal to 45°Cno93.611yes
125438motile2+flagellatedAbility to perform flagellated movementno92no

External links

@ref: 8679

culture collection no.: DSM 20272, ATCC 4875, NCDO 570, NCIB 8070, JCM 6432, IFO 12425, NBRC 12425, NCIMB 8070, VPI 10402, CIP 53.164

straininfo link

  • @ref: 81847
  • straininfo: 8651

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology15977254Enhanced propionic acid fermentation by Propionibacterium acidipropionici mutant obtained by adaptation in a fibrous-bed bioreactor.Suwannakham S, Yang STBiotechnol Bioeng10.1002/bit.204732005Adenosine Triphosphatases/metabolism, *Bioreactors, Biotechnology/*methods, Cell Membrane/enzymology, Cells, Immobilized/enzymology, Fermentation, Mutation, Propionates/*metabolism, Propionibacterium/*enzymology/*geneticsMetabolism
Metabolism22481002Efficient utilization of hemicellulose hydrolysate for propionic acid production using Propionibacterium acidipropionici.Liu Z, Ma C, Gao C, Xu PBioresour Technol10.1016/j.biortech.2012.02.1182012Carbohydrate Metabolism/*physiology, Hydrolysis, Polysaccharides/*chemistry, Propionates/*isolation & purification/*metabolism, Propionibacterium/*metabolismBiotechnology
Metabolism23083487The genome sequence of Propionibacterium acidipropionici provides insights into its biotechnological and industrial potential.Parizzi LP, Grassi MC, Llerena LA, Carazzolle MF, Queiroz VL, Lunardi I, Zeidler AF, Teixeira PJ, Mieczkowski P, Rincones J, Pereira GABMC Genomics10.1186/1471-2164-13-5622012Acetate-CoA Ligase/genetics/metabolism, Bacterial Proteins/*genetics/metabolism, Base Composition, Base Sequence, Carbon Dioxide/metabolism, *Genome, Bacterial, *Industrial Microbiology, Metabolic Networks and Pathways, Molecular Sequence Data, Plasmids, Propionates/*metabolism, Propionibacterium/*genetics/*metabolism, Pyruvate Carboxylase/genetics/metabolism, beta-Fructofuranosidase/genetics/metabolismGenetics
Biotechnology24627047Development of an industrializable fermentation process for propionic acid production.Stowers CC, Cox BM, Rodriguez BAJ Ind Microbiol Biotechnol10.1007/s10295-014-1423-62014Bioreactors/microbiology, *Fermentation, Industrial Microbiology/economics, Propionates/*metabolism, Propionibacterium/*metabolismMetabolism
Metabolism25257628High cell density propionic acid fermentation with an acid tolerant strain of Propionibacterium acidipropionici.Wang Z, Jin Y, Yang STBiotechnol Bioeng10.1002/bit.254662014Batch Cell Culture Techniques, Bioreactors/*microbiology, Fermentation, Glucose/metabolism, Hydrogen-Ion Concentration, Kinetics, Propionates/*metabolism, Propionibacterium/*metabolism/*physiologyEnzymology
Biotechnology25852990Microbial production of Propionic and Succinic acid from Sorbitol using Propionibacterium acidipropionici.Duarte JC, Valenca GP, Moran PJ, Rodrigues JAAMB Express10.1186/s13568-015-0095-62015

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8679Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20272)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20272
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
42208Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17077
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68380Automatically annotated from API rID32A
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
81847Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID8651.1StrainInfo: A central database for resolving microbial strain identifiers
116014Curators of the CIPCollection of Institut Pasteur (CIP 53.164)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2053.164
125438Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann10.1101/2024.08.12.607695Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets
125439Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardyhttps://github.com/GenomeNet/deepGdeepG: Deep Learning for Genome Sequence Data. R package version 0.3.1