Strain identifier
BacDive ID: 12626
Type strain:
Species: Microlunatus ginsengisoli
Strain history: CIP <- 2007, DSMZ <- S. T. Lee <- W. T. Im, Korea Ad. Inst. Technol., Guseong-dong, Daejon, Korea: strain Gsoil 633
NCBI tax ID(s): 363863 (species)
General
@ref: 7279
BacDive-ID: 12626
DSM-Number: 17942
keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, coccus-shaped
description: Microlunatus ginsengisoli DSM 17942 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from soil, ginseng field.
NCBI tax id
- NCBI tax id: 363863
- Matching level: species
strain history
@ref | history |
---|---|
7279 | <- Sung-Taik Lee <- Wan-Taek Im, Gsoil 633 |
67770 | W.-T. Im Gsoil 633. |
67771 | <- ST Lee, KAIST |
119627 | CIP <- 2007, DSMZ <- S. T. Lee <- W. T. Im, Korea Ad. Inst. Technol., Guseong-dong, Daejon, Korea: strain Gsoil 633 |
doi: 10.13145/bacdive12626.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Propionibacteriales
- family: Propionibacteriaceae
- genus: Microlunatus
- species: Microlunatus ginsengisoli
- full scientific name: Microlunatus ginsengisoli Cui et al. 2007
@ref: 7279
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Propionibacteriaceae
genus: Microlunatus
species: Microlunatus ginsengisoli
full scientific name: Microlunatus ginsengisoli Cui et al. 2007
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell width | cell shape | motility |
---|---|---|---|---|
31819 | positive | 0.5-0.8 µm | coccus-shaped | no |
67771 | positive | |||
119627 | positive | coccus-shaped | no |
colony morphology
- @ref: 19757
- colony color: Honey yellow (1005)
- incubation period: 10-14 days
- medium used: ISP 3
multicellular morphology
- @ref: 19757
- forms multicellular complex: no
- medium name: ISP 3
pigmentation
- @ref: 31819
- production: yes
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
7279 | MICROLUNATUS MEDIUM (DSMZ Medium 776) | yes | https://mediadive.dsmz.de/medium/776 | Name: MICROLUNATUS MEDIUM (DSMZ Medium 776) Composition: KH2PO4 0.5 g/l Na glutamate 0.5 g/l Yeast extract 0.5 g/l Peptone 0.5 g/l Glucose 0.5 g/l (NH4)2SO4 0.1 g/l MgSO4 x 7 H2O 0.1 g/l Distilled water |
19757 | ISP 3 | yes | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |
33312 | MEDIUM 135 - for Microlunatus phosphovorus and Microsphaera multipartita | yes | Distilled water make up to (1000.000 ml);Magnesium sulphate heptahydrate (0.100 g);Agar (18.000 g);Glucose (0.500 g);Yeast extract (0.500 g);Ammonium sulphate (0.100 g);Peptone (0.500 g);Potassium di-hydrogen phosphate (0.440 g);Sodium glutamate (0.500 g) | |
119627 | CIP Medium 135 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=135 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
7279 | positive | growth | 28 | mesophilic |
19757 | positive | optimum | 28 | mesophilic |
31819 | positive | growth | 16-37 | |
31819 | positive | optimum | 20-30 | |
33312 | positive | growth | 25 | mesophilic |
67770 | positive | growth | 28 | mesophilic |
67771 | positive | growth | 30 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
31819 | positive | growth | 5.5-8.5 | alkaliphile |
31819 | positive | optimum | 7 |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
31819 | aerobe |
67771 | aerobe |
spore formation
- @ref: 31819
- spore formation: no
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
31819 | NaCl | positive | growth | 04-05 % |
31819 | NaCl | positive | optimum | 4.5 % |
observation
- @ref: 67770
- observation: quinones: MK-9(H4)
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
31819 | 15963 | ribitol | + | carbon source |
31819 | 16449 | alanine | + | carbon source |
31819 | 22653 | asparagine | + | carbon source |
31819 | 35391 | aspartate | + | carbon source |
31819 | 23652 | dextrin | + | carbon source |
31819 | 28757 | fructose | + | carbon source |
31819 | 27570 | histidine | + | carbon source |
31819 | 24996 | lactate | + | carbon source |
31819 | 25115 | malate | + | carbon source |
31819 | 28044 | phenylalanine | + | carbon source |
31819 | 26271 | proline | + | carbon source |
31819 | 17272 | propionate | + | carbon source |
31819 | 16634 | raffinose | + | carbon source |
31819 | 26546 | rhamnose | + | carbon source |
31819 | 33942 | ribose | + | carbon source |
31819 | 17814 | salicin | + | carbon source |
31819 | 30911 | sorbitol | + | carbon source |
31819 | 17992 | sucrose | + | carbon source |
31819 | 27082 | trehalose | + | carbon source |
31819 | 31011 | valerate | + | carbon source |
31819 | 18222 | xylose | + | carbon source |
31819 | 17632 | nitrate | + | reduction |
119627 | 17632 | nitrate | + | reduction |
119627 | 16301 | nitrite | - | reduction |
68379 | 17632 | nitrate | - | reduction |
68379 | 4853 | esculin | + | hydrolysis |
68379 | 16199 | urea | + | hydrolysis |
68379 | 5291 | gelatin | + | hydrolysis |
68379 | 17634 | D-glucose | - | fermentation |
68379 | 16988 | D-ribose | - | fermentation |
68379 | 65327 | D-xylose | - | fermentation |
68379 | 16899 | D-mannitol | - | fermentation |
68379 | 17306 | maltose | - | fermentation |
68379 | 17716 | lactose | - | fermentation |
68379 | 17992 | sucrose | + | fermentation |
68379 | 28087 | glycogen | - | fermentation |
metabolite production
- @ref: 119627
- Chebi-ID: 35581
- metabolite: indole
- production: no
enzymes
@ref | value | activity | ec |
---|---|---|---|
31819 | catalase | + | 1.11.1.6 |
68379 | gelatinase | + | |
68379 | urease | + | 3.5.1.5 |
68379 | beta-glucosidase | + | 3.2.1.21 |
68379 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
68379 | alpha-glucosidase | + | 3.2.1.20 |
68379 | beta-galactosidase | + | 3.2.1.23 |
68379 | beta-glucuronidase | + | 3.2.1.31 |
68379 | alkaline phosphatase | + | 3.1.3.1 |
68379 | pyrrolidonyl arylamidase | - | 3.4.19.3 |
68379 | pyrazinamidase | - | 3.5.1.B15 |
119627 | oxidase | - | |
119627 | catalase | + | 1.11.1.6 |
119627 | urease | + | 3.5.1.5 |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | - | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | - | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | beta-galactosidase | + | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | + | 3.2.1.20 |
68382 | beta-glucosidase | + | 3.2.1.21 |
API coryne
@ref | NIT | PYZ | PYRA | PAL | beta GUR | beta GAL | alpha GLU | beta NAG | ESC | URE | GEL | GLU | RIB | XYL | MAN | MAL | LAC | SAC | GLYG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
19757 | - | - | - | + | + | + | + | + | + | + | + | - | - | - | - | - | - | + | - |
API zym
@ref | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase | Control |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
19757 | + | - | + | - | + | - | - | - | - | + | - | + | + | - | + | + | + | + | + | |
119627 | - | + | - | - | + | - | - | - | - | + | + | - | + | - | + | + | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
7279 | soil, ginseng field | Pocheon Province | Republic of Korea | KOR | Asia |
67770 | Soil from a ginseng field | Pocheon Province | Republic of Korea | KOR | Asia |
67771 | From soil of the ginseng field | Republic of Korea | KOR | Asia | |
119627 | Environment, Soil, ginseng field | Pocheon | Republic of Korea | KOR | Asia |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Agriculture | #Field |
#Environmental | #Terrestrial | #Soil |
#Host | #Plants | #Herbaceous plants (Grass,Crops) |
taxonmaps
- @ref: 69479
- File name: preview.99_132832.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_72;96_3710;97_4562;98_20755;99_132832&stattab=map
- Last taxonomy: Microlunatus ginsengisoli subclade
- 16S sequence: AB245389
- Sequence Identity:
- Total samples: 558
- soil counts: 427
- aquatic counts: 28
- animal counts: 94
- plant counts: 9
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
7279 | 1 | Risk group (German classification) |
19757 | 1 | Risk group (German classification) |
119627 | 1 | Risk group (French classification) |
Sequence information
16S sequences
- @ref: 7279
- description: Microlunatus ginsengisoli gene for 16S rRNA, partial sequence, strain: Gsoil 633
- accession: AB245389
- length: 1472
- database: ena
- NCBI tax ID: 363863
GC content
@ref | GC-content | method |
---|---|---|
7279 | 69.8 | |
67770 | 69.8 | high performance liquid chromatography (HPLC) |
External links
@ref: 7279
culture collection no.: DSM 17942, CIP 109594, KCTC 13940, Gsoil 633, JCM 16929, JCM 15306
straininfo link
- @ref: 81840
- straininfo: 302767
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 17392192 | Microlunatus ginsengisoli sp. nov., isolated from soil of a ginseng field. | Cui YS, Im WT, Yin CR, Yang DC, Lee ST | Int J Syst Evol Microbiol | 10.1099/ijs.0.64395-0 | 2007 | DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Korea, Molecular Sequence Data, Panax/*microbiology, Phylogeny, Propionibacteriaceae/*classification/genetics/*isolation & purification/physiology, RNA, Ribosomal, 16S/genetics, *Soil Microbiology | Genetics |
Phylogeny | 19946055 | Microlunatus parietis sp. nov., isolated from an indoor wall. | Kampfer P, Schafer J, Lodders N, Martin K | Int J Syst Evol Microbiol | 10.1099/ijs.0.020115-0 | 2009 | Bacterial Typing Techniques, Cell Wall/chemistry, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Diaminopimelic Acid/analysis, *Environmental Microbiology, Fatty Acids, Molecular Sequence Data, Mycolic Acids/analysis, Phospholipids/analysis, Phylogeny, Propionibacteriaceae/chemistry/*classification/genetics/*isolation & purification, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA | Genetics |
Phylogeny | 32886594 | Microlunatus elymi sp. nov., a novel actinobacterium isolated from rhizospheric soil of the wild plant Elymus tsukushiensis. | Son JS, Lee SY, Hwang YJ, Shin JH, Ghim SY | Int J Syst Evol Microbiol | 10.1099/ijsem.0.004428 | 2020 | Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Elymus/*microbiology, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Propionibacteriaceae/*classification/isolation & purification, RNA, Ribosomal, 16S/genetics, Republic of Korea, *Rhizosphere, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistry | Transcriptome |
Reference
@id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
---|---|---|---|---|---|---|
7279 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17942) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-17942 | |||
19757 | Wink, J. | http://www.dsmz.de/microorganisms/wink_pdf/DSM17942.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
31819 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 28086 | 28776041 | |
33312 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/7355 | ||||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
67771 | Curators of the KCTC | https://kctc.kribb.re.kr/En/Kctc | ||||
68379 | Automatically annotated from API Coryne | |||||
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
81840 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID302767.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
119627 | Curators of the CIP | Collection of Institut Pasteur (CIP 109594) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109594 |