Strain identifier

BacDive ID: 12619

Type strain: Yes

Species: Microlunatus sagamiharensis

Strain Designation: FB2, S20_27

Strain history: <- K. Iwai, BioFrontier Laboratories, Kyowa Hakko Kogyo Co., Ltd.; S20_27

NCBI tax ID(s): 546874 (species)

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General

@ref: 15909

BacDive-ID: 12619

DSM-Number: 21743

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, coccus-shaped

description: Microlunatus sagamiharensis FB2 is an aerobe, mesophilic, coccus-shaped bacterium that was isolated from web of the spider Agelena silvatica.

NCBI tax id

  • NCBI tax id: 546874
  • Matching level: species

strain history

  • @ref: 15909
  • history: <- K. Iwai, BioFrontier Laboratories, Kyowa Hakko Kogyo Co., Ltd.; S20_27

doi: 10.13145/bacdive12619.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Propionibacteriales
  • family: Propionibacteriaceae
  • genus: Microlunatus
  • species: Microlunatus sagamiharensis
  • full scientific name: Microlunatus sagamiharensis (Iwai et al. 2010) Nouioui et al. 2018
  • synonyms

    • @ref: 20215
    • synonym: Friedmanniella sagamiharensis

@ref: 15909

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Propionibacteriaceae

genus: Microlunatus

species: Microlunatus sagamiharensis

full scientific name: Microlunatus sagamiharensis (Iwai et al. 2010) Nouioui et al. 2018

strain designation: FB2, S20_27

type strain: yes

Morphology

cell morphology

@refcell widthcell shapemotilityconfidencegram stain
291680.85 µmcoccus-shaped
69480no95.299
69480100positive

pigmentation

  • @ref: 29168
  • production: yes

Culture and growth conditions

culture medium

  • @ref: 15909
  • name: PLATE COUNT AGAR (DSMZ Medium 464)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/464
  • composition: Name: PLATE COUNT AGAR (DSMZ Medium 464) Composition: Agar 15.0 g/l Tryptone 5.0 g/l Yeast extract 2.5 g/l Dextrose 1.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
15909positivegrowth25-28mesophilic
29168positivegrowth18-32
29168positiveoptimum26.5mesophilic

culture pH

@refabilitytypepHPH range
29168positivegrowth05-09alkaliphile
29168positiveoptimum7.35

Physiology and metabolism

oxygen tolerance

  • @ref: 29168
  • oxygen tolerance: aerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.787

halophily

  • @ref: 29168
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-6 %

observation

  • @ref: 29168
  • observation: aggregates in clumps

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2916822599arabinose+carbon source
2916828757fructose+carbon source
2916828260galactose+carbon source
2916817234glucose+carbon source
2916829864mannitol+carbon source
2916817268myo-inositol+carbon source
2916816634raffinose+carbon source
2916826546rhamnose+carbon source
2916833942ribose+carbon source
2916817992sucrose+carbon source
2916818222xylose+carbon source

enzymes

@refvalueactivityec
29168gelatinase+
29168urease+3.5.1.5

Isolation, sampling and environmental information

isolation

  • @ref: 15909
  • sample type: web of the spider Agelena silvatica
  • host species: Agelena silvatica
  • geographic location: Kanagawa Prefecture
  • country: Japan
  • origin.country: JPN
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Host#Arthropoda#Insecta
#Host Body Product#Fluids

Safety information

risk assessment

  • @ref: 15909
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 15909
  • description: Friedmanniella sagamiharensis gene for 16S rRNA, partial sequence
  • accession: AB445456
  • length: 1495
  • database: ena
  • NCBI tax ID: 546874

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Microlunatus sagamiharensis DSM 21743GCA_900105785chromosomencbi546874
66792Friedmanniella sagamiharensis strain DSM 21743546874.3completepatric546874
66792Microlunatus sagamiharensis DSM 217432634166345draftimg546874

GC content

  • @ref: 15909
  • GC-content: 75

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno58no
gram-positiveyes87.952no
anaerobicno99.19yes
halophileno95.012no
spore-formingno85.482no
glucose-utilyes87.052no
thermophileno98.932yes
aerobicyes91.273yes
motileno94.604no
glucose-fermentno86.259no
flagellatedno98.533no

External links

@ref: 15909

culture collection no.: DSM 21743, NBRC 104965, KY 13154

straininfo link

  • @ref: 81833
  • straininfo: 403191

literature

  • topic: Phylogeny
  • Pubmed-ID: 19648329
  • title: Friedmanniella luteola sp. nov., Friedmanniella lucida sp. nov., Friedmanniella okinawensis sp. nov. and Friedmaniella sagamiharensis sp. nov., isolated from spiders.
  • authors: Iwai K, Aisaka K, Suzuki M
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.007815-0
  • year: 2009
  • mesh: Actinomycetales/*classification/genetics/*isolation & purification/metabolism, Animals, Base Composition, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Spiders/*microbiology
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmedjournal
15909Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21743)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21743
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29168Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2559228776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updates33211880Nucleic Acids Res. 49: D498-D508 2020
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
81833Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID403191.1StrainInfo: A central database for resolving microbial strain identifiers