Strain identifier
BacDive ID: 12619
Type strain:
Species: Microlunatus sagamiharensis
Strain Designation: FB2, S20_27
Strain history: <- K. Iwai, BioFrontier Laboratories, Kyowa Hakko Kogyo Co., Ltd.; S20_27
NCBI tax ID(s): 546874 (species)
General
@ref: 15909
BacDive-ID: 12619
DSM-Number: 21743
keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, coccus-shaped
description: Microlunatus sagamiharensis FB2 is an aerobe, mesophilic, coccus-shaped bacterium that was isolated from web of the spider Agelena silvatica.
NCBI tax id
- NCBI tax id: 546874
- Matching level: species
strain history
- @ref: 15909
- history: <- K. Iwai, BioFrontier Laboratories, Kyowa Hakko Kogyo Co., Ltd.; S20_27
doi: 10.13145/bacdive12619.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Propionibacteriales
- family: Propionibacteriaceae
- genus: Microlunatus
- species: Microlunatus sagamiharensis
- full scientific name: Microlunatus sagamiharensis (Iwai et al. 2010) Nouioui et al. 2018
synonyms
- @ref: 20215
- synonym: Friedmanniella sagamiharensis
@ref: 15909
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Propionibacteriaceae
genus: Microlunatus
species: Microlunatus sagamiharensis
full scientific name: Microlunatus sagamiharensis (Iwai et al. 2010) Nouioui et al. 2018
strain designation: FB2, S20_27
type strain: yes
Morphology
cell morphology
@ref | cell width | cell shape | motility | confidence | gram stain |
---|---|---|---|---|---|
29168 | 0.85 µm | coccus-shaped | |||
69480 | no | 95.299 | |||
69480 | 100 | positive |
pigmentation
- @ref: 29168
- production: yes
Culture and growth conditions
culture medium
- @ref: 15909
- name: PLATE COUNT AGAR (DSMZ Medium 464)
- growth: yes
- link: https://mediadive.dsmz.de/medium/464
- composition: Name: PLATE COUNT AGAR (DSMZ Medium 464) Composition: Agar 15.0 g/l Tryptone 5.0 g/l Yeast extract 2.5 g/l Dextrose 1.0 g/l Distilled water
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
15909 | positive | growth | 25-28 | mesophilic |
29168 | positive | growth | 18-32 | |
29168 | positive | optimum | 26.5 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
29168 | positive | growth | 05-09 | alkaliphile |
29168 | positive | optimum | 7.35 |
Physiology and metabolism
oxygen tolerance
- @ref: 29168
- oxygen tolerance: aerobe
spore formation
- @ref: 69480
- spore formation: no
- confidence: 99.787
halophily
- @ref: 29168
- salt: NaCl
- growth: positive
- tested relation: growth
- concentration: 0-6 %
observation
- @ref: 29168
- observation: aggregates in clumps
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
29168 | 22599 | arabinose | + | carbon source |
29168 | 28757 | fructose | + | carbon source |
29168 | 28260 | galactose | + | carbon source |
29168 | 17234 | glucose | + | carbon source |
29168 | 29864 | mannitol | + | carbon source |
29168 | 17268 | myo-inositol | + | carbon source |
29168 | 16634 | raffinose | + | carbon source |
29168 | 26546 | rhamnose | + | carbon source |
29168 | 33942 | ribose | + | carbon source |
29168 | 17992 | sucrose | + | carbon source |
29168 | 18222 | xylose | + | carbon source |
enzymes
@ref | value | activity | ec |
---|---|---|---|
29168 | gelatinase | + | |
29168 | urease | + | 3.5.1.5 |
Isolation, sampling and environmental information
isolation
- @ref: 15909
- sample type: web of the spider Agelena silvatica
- host species: Agelena silvatica
- geographic location: Kanagawa Prefecture
- country: Japan
- origin.country: JPN
- continent: Asia
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Host | #Arthropoda | #Insecta |
#Host Body Product | #Fluids |
Safety information
risk assessment
- @ref: 15909
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 15909
- description: Friedmanniella sagamiharensis gene for 16S rRNA, partial sequence
- accession: AB445456
- length: 1495
- database: ena
- NCBI tax ID: 546874
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Microlunatus sagamiharensis DSM 21743 | GCA_900105785 | chromosome | ncbi | 546874 |
66792 | Friedmanniella sagamiharensis strain DSM 21743 | 546874.3 | complete | patric | 546874 |
66792 | Microlunatus sagamiharensis DSM 21743 | 2634166345 | draft | img | 546874 |
GC content
- @ref: 15909
- GC-content: 75
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 58 | no |
gram-positive | yes | 87.952 | no |
anaerobic | no | 99.19 | yes |
halophile | no | 95.012 | no |
spore-forming | no | 85.482 | no |
glucose-util | yes | 87.052 | no |
thermophile | no | 98.932 | yes |
aerobic | yes | 91.273 | yes |
motile | no | 94.604 | no |
glucose-ferment | no | 86.259 | no |
flagellated | no | 98.533 | no |
External links
@ref: 15909
culture collection no.: DSM 21743, NBRC 104965, KY 13154
straininfo link
- @ref: 81833
- straininfo: 403191
literature
- topic: Phylogeny
- Pubmed-ID: 19648329
- title: Friedmanniella luteola sp. nov., Friedmanniella lucida sp. nov., Friedmanniella okinawensis sp. nov. and Friedmaniella sagamiharensis sp. nov., isolated from spiders.
- authors: Iwai K, Aisaka K, Suzuki M
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.007815-0
- year: 2009
- mesh: Actinomycetales/*classification/genetics/*isolation & purification/metabolism, Animals, Base Composition, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Spiders/*microbiology
- topic2: Metabolism
Reference
@id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed | journal |
---|---|---|---|---|---|---|---|
15909 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21743) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-21743 | ||||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | ||||
29168 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 25592 | 28776041 | ||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | ||||
66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | 33211880 | Nucleic Acids Res. 49: D498-D508 2020 | ||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | ||||
81833 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID403191.1 | StrainInfo: A central database for resolving microbial strain identifiers |