Strain identifier

BacDive ID: 12617

Type strain: Yes

Species: Friedmanniella luteola

Strain Designation: A34_1, FA1

Strain history: <- K. Iwai, BioFrontier Laboratories, Kyowa Hakko Kogyo Co., Ltd.; A34_1

NCBI tax ID(s): 546871 (species)

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General

@ref: 15904

BacDive-ID: 12617

DSM-Number: 21741

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, coccus-shaped

description: Friedmanniella luteola A34_1 is an aerobe, mesophilic, coccus-shaped bacterium that was isolated from surface of the spider Diaea subdola.

NCBI tax id

  • NCBI tax id: 546871
  • Matching level: species

strain history

  • @ref: 15904
  • history: <- K. Iwai, BioFrontier Laboratories, Kyowa Hakko Kogyo Co., Ltd.; A34_1

doi: 10.13145/bacdive12617.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Propionibacteriales
  • family: Propionibacteriaceae
  • genus: Friedmanniella
  • species: Friedmanniella luteola
  • full scientific name: Friedmanniella luteola Iwai et al. 2010

  • synonyms

    • @ref: 20215
    • synonym: Microlunatus luteolus

@ref: 15904

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Propionibacteriaceae

genus: Friedmanniella

species: Friedmanniella luteola

full scientific name: Friedmanniella luteola Iwai et al. 2010

strain designation: A34_1, FA1

type strain: yes

Morphology

cell morphology

@refcell widthcell shapemotilityconfidencegram stain
291681.35 µmcoccus-shaped
69480no93.912
69480100positive

colony morphology

@refcolony colorincubation periodmedium used
21357Golden yellow (1014)10-14 daysISP 2
21357Ivory (1014)10-14 daysISP 3
21357Traffic yellow (1023)10-14 daysISP 4
21357Saffron yellow (1017)10-14 daysISP 5
21357Golden yellow (1004)10-14 daysISP 7
21357Saffron yellow (1017)10-14 dayssuter with tyrosine
21357Saffron yellow (1017)10-14 dayssuter without tyrosine

multicellular morphology

@refforms multicellular complexmedium name
21357noISP 2
21357noISP 3
21357noISP 4
21357noISP 5
21357noISP 7
21357noSuter with tyrosine
21357noSuter without tyrosine

pigmentation

  • @ref: 29168
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
15904R2A MEDIUM (DSMZ Medium 830)yeshttps://mediadive.dsmz.de/medium/830Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
21357ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
21357ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
21357ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
21357ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
21357ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
21357suter with tyrosineyesName: Synthetically suter medium (5337) with and without tyrosine - see Kutzner,H.J., R. M. Kroppensted and F. Korn-Wendisch. (1986) Composition: Glycerol 15.0 g/l Tyrosine 1.0 (optional, see preparation comment) L-arginine 5.0 g/l L-glutamic acid 5.0 g/l L-methionine 0.3 g/l L-isoleucine 0.3 g/l K2HPO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l Agar 20.0 g/l Trace element solution (5341) 1.0 ml Composition of Trace element solution (5341): CuSO4 x 5 H2O 10.0 g/l CaCl2 x 2 H2O 10.0 g/l FeSO4 x 7 H2O 10.0 g/l ZnSO4 x 7 H2O 10.0 g/l MnSO4 x 7 H2O 40.0 g/l Preparation: Control medium is prepared without tyrosine Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigment Organisms: All Actinomycetes
21357suter without tyrosineyesName: Synthetically suter medium (5337) with and without tyrosine - see Kutzner,H.J., R. M. Kroppensted and F. Korn-Wendisch. (1986) Composition: Glycerol 15.0 g/l Tyrosine 1.0 (optional, see preparation comment) L-arginine 5.0 g/l L-glutamic acid 5.0 g/l L-methionine 0.3 g/l L-isoleucine 0.3 g/l K2HPO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l Agar 20.0 g/l Trace element solution (5341) 1.0 ml Composition of Trace element solution (5341): CuSO4 x 5 H2O 10.0 g/l CaCl2 x 2 H2O 10.0 g/l FeSO4 x 7 H2O 10.0 g/l ZnSO4 x 7 H2O 10.0 g/l MnSO4 x 7 H2O 40.0 g/l Preparation: Control medium is prepared without tyrosine Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigment Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperaturerange
15904positivegrowth25mesophilic
29168positivegrowth18-28
29168positiveoptimum26mesophilic

culture pH

@refabilitytypepHPH range
29168positivegrowth5.3-8.5alkaliphile
29168positiveoptimum6.85

Physiology and metabolism

oxygen tolerance

  • @ref: 29168
  • oxygen tolerance: aerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 98.807

halophily

@refsaltgrowthtested relationconcentration
21357NaClpositivegrowth0 %
29168NaClpositivegrowth0-2 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2135717234glucose-
2135722599arabinose-
2135717992sucrose-
2135718222xylose-
2135717268myo-inositol-
2135737684mannose-
2135728757fructose-
2135726546rhamnose-
2135716634raffinose-
2135762968cellulose-
2916822599arabinose+carbon source
2916828757fructose+carbon source
2916828260galactose+carbon source
2916817268myo-inositol+carbon source
2916816634raffinose+carbon source
6837917632nitrate-reduction
6837916199urea-hydrolysis
683795291gelatin-hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837928087glycogen-fermentation

enzymes

@refvalueactivityec
29168catalase+1.11.1.6
29168gelatinase+
29168urease+3.5.1.5
68379gelatinase-
68379urease-3.5.1.5
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase-3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase+3.2.1.24

API coryne

@refNITPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACGLYG
21357-------+/----------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
21357++++/-+-+/---+/-+-+/--++/-+++/-

Isolation, sampling and environmental information

isolation

  • @ref: 15904
  • sample type: surface of the spider Diaea subdola
  • host species: Diaea subdola
  • geographic location: Yamanashi
  • country: Japan
  • origin.country: JPN
  • continent: Asia

isolation source categories

  • Cat1: #Host
  • Cat2: #Arthropoda
  • Cat3: #Insecta

taxonmaps

  • @ref: 69479
  • File name: preview.99_21828.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_72;96_5617;97_13378;98_16466;99_21828&stattab=map
  • Last taxonomy: Friedmanniella luteola
  • 16S sequence: AB445453
  • Sequence Identity:
  • Total samples: 879
  • soil counts: 432
  • aquatic counts: 81
  • animal counts: 347
  • plant counts: 19

Safety information

risk assessment

  • @ref: 15904
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 15904
  • description: Friedmanniella luteola gene for 16S rRNA, partial sequence
  • accession: AB445453
  • length: 1458
  • database: ena
  • NCBI tax ID: 546871

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Friedmanniella luteola DSM 21741GCA_900105065chromosomencbi546871
66792Friedmanniella luteola strain DSM 21741546871.3completepatric546871
66792Friedmanniella luteola DSM 217412630969010draftimg546871

GC content

@refGC-contentmethod
1590472high performance liquid chromatography (HPLC)
2916872

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes82no
gram-positiveyes87.273no
anaerobicno99.254yes
halophileno94.91yes
spore-formingno76.636no
glucose-utilyes83.059yes
thermophileno98.877yes
aerobicyes93.129no
motileno92.422no
flagellatedno98.422no
glucose-fermentno90.786no

External links

@ref: 15904

culture collection no.: DSM 21741, NBRC 104963, KY 13132

straininfo link

  • @ref: 81831
  • straininfo: 401004

literature

  • topic: Phylogeny
  • Pubmed-ID: 19648329
  • title: Friedmanniella luteola sp. nov., Friedmanniella lucida sp. nov., Friedmanniella okinawensis sp. nov. and Friedmaniella sagamiharensis sp. nov., isolated from spiders.
  • authors: Iwai K, Aisaka K, Suzuki M
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.007815-0
  • year: 2009
  • mesh: Actinomycetales/*classification/genetics/*isolation & purification/metabolism, Animals, Base Composition, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Spiders/*microbiology
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmedjournal
15904Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21741)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21741
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
21357Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM21741.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
29168Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2559228776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updates33211880Nucleic Acids Res. 49: D498-D508 2020
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
81831Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID401004.1StrainInfo: A central database for resolving microbial strain identifiers