Strain identifier

BacDive ID: 12616

Type strain: Yes

Species: Microlunatus lucidus

Strain Designation: FA2, M2_8

Strain history: <- K. Iwai, BioFrontier Laboratories, Kyowa Hakko Kogyo Co., Ltd.; M2_8

NCBI tax ID(s): 546872 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 15908

BacDive-ID: 12616

DSM-Number: 21742

keywords: 16S sequence, Bacteria, aerobe

description: Microlunatus lucidus FA2 is an aerobe bacterium that was isolated from web of the spider Agelena silvatica.

NCBI tax id

  • NCBI tax id: 546872
  • Matching level: species

strain history

  • @ref: 15908
  • history: <- K. Iwai, BioFrontier Laboratories, Kyowa Hakko Kogyo Co., Ltd.; M2_8

doi: 10.13145/bacdive12616.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Propionibacteriales
  • family: Propionibacteriaceae
  • genus: Microlunatus
  • species: Microlunatus lucidus
  • full scientific name: Microlunatus lucidus (Iwai et al. 2010) Nouioui et al. 2018
  • synonyms

    • @ref: 20215
    • synonym: Friedmanniella lucida

@ref: 15908

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Propionibacteriaceae

genus: Microlunatus

species: Microlunatus lucidus

full scientific name: Microlunatus lucidus (Iwai et al. 2010) Nouioui et al. 2018

strain designation: FA2, M2_8

type strain: yes

Morphology

cell morphology

  • @ref: 29168
  • motility: no

colony morphology

@refcolony colorincubation periodmedium used
21358Honey lemon (1005)10-14 daysISP 2
21358Lemon yellow (1012)10-14 daysISP 3
21358Lemon yellow (1012)10-14 daysISP 7
2135810-14 dayssuter with tyrosine
2135810-14 dayssuter without tyrosine

multicellular morphology

@refforms multicellular complexmedium name
21358noISP 2
21358noISP 3
21358
21358noISP 7
21358noSuter with tyrosine
21358noSuter without tyrosine

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
15908R2A MEDIUM (DSMZ Medium 830)yeshttps://mediadive.dsmz.de/medium/830Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
21358ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
21358ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
21358ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
21358suter with tyrosineyesName: Synthetically suter medium (5337) with and without tyrosine - see Kutzner,H.J., R. M. Kroppensted and F. Korn-Wendisch. (1986) Composition: Glycerol 15.0 g/l Tyrosine 1.0 (optional, see preparation comment) L-arginine 5.0 g/l L-glutamic acid 5.0 g/l L-methionine 0.3 g/l L-isoleucine 0.3 g/l K2HPO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l Agar 20.0 g/l Trace element solution (5341) 1.0 ml Composition of Trace element solution (5341): CuSO4 x 5 H2O 10.0 g/l CaCl2 x 2 H2O 10.0 g/l FeSO4 x 7 H2O 10.0 g/l ZnSO4 x 7 H2O 10.0 g/l MnSO4 x 7 H2O 40.0 g/l Preparation: Control medium is prepared without tyrosine Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigment Organisms: All Actinomycetes
21358suter without tyrosineyesName: Synthetically suter medium (5337) with and without tyrosine - see Kutzner,H.J., R. M. Kroppensted and F. Korn-Wendisch. (1986) Composition: Glycerol 15.0 g/l Tyrosine 1.0 (optional, see preparation comment) L-arginine 5.0 g/l L-glutamic acid 5.0 g/l L-methionine 0.3 g/l L-isoleucine 0.3 g/l K2HPO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l Agar 20.0 g/l Trace element solution (5341) 1.0 ml Composition of Trace element solution (5341): CuSO4 x 5 H2O 10.0 g/l CaCl2 x 2 H2O 10.0 g/l FeSO4 x 7 H2O 10.0 g/l ZnSO4 x 7 H2O 10.0 g/l MnSO4 x 7 H2O 40.0 g/l Preparation: Control medium is prepared without tyrosine Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigment Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperature
15908positivegrowth22-28
29168positivegrowth18-32
29168positiveoptimum22-28

culture pH

@refabilitytypepHPH range
29168positivegrowth05-09alkaliphile
29168positiveoptimum06-07

Physiology and metabolism

oxygen tolerance

  • @ref: 29168
  • oxygen tolerance: aerobe

halophily

@refsaltgrowthtested relationconcentration
21358NaClpositivegrowth0 %
29168NaClpositivegrowth0-2 %
29168NaClpositiveoptimum0-2 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2135817234glucose+/-
2135822599arabinose+/-
2135817992sucrose+/-
2135818222xylose+/-
2135817268myo-inositol+/-
2135837684mannose+/-
2135828757fructose+/-
2135826546rhamnose+/-
2135816634raffinose-
2135862968cellulose-
2916817234glucose+carbon source
2916829864mannitol+carbon source
2916816634raffinose+carbon source
2916817992sucrose+carbon source
683794853esculin+hydrolysis
6837916199urea-hydrolysis
683795291gelatin-hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837928087glycogen-fermentation

enzymes

@refvalueactivityec
29168catalase+1.11.1.6
29168urease+3.5.1.5
68379gelatinase-
68379urease-3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase-3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382alpha-chymotrypsin-3.4.21.1
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACGLYG
21358-------+---------
21358+------+---------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
21358----+++/-+/--+/-+-+/--+++--

Isolation, sampling and environmental information

isolation

  • @ref: 15908
  • sample type: web of the spider Agelena silvatica
  • host species: Agelena silvatica
  • geographic location: Mitake ravine
  • country: Japan
  • origin.country: JPN
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Host#Arthropoda#Insecta
#Host Body Product#Fluids

Safety information

risk assessment

  • @ref: 15908
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 15908
  • description: Friedmanniella lucida gene for 16S rRNA, partial sequence
  • accession: AB445454
  • length: 1420
  • database: ena
  • NCBI tax ID: 546872

GC content

@refGC-contentmethod
1590873high performance liquid chromatography (HPLC)
2916873

External links

@ref: 15908

culture collection no.: DSM 21742, NBRC 104964, KY 13138

straininfo link

  • @ref: 81830
  • straininfo: 400181

literature

  • topic: Phylogeny
  • Pubmed-ID: 19648329
  • title: Friedmanniella luteola sp. nov., Friedmanniella lucida sp. nov., Friedmanniella okinawensis sp. nov. and Friedmaniella sagamiharensis sp. nov., isolated from spiders.
  • authors: Iwai K, Aisaka K, Suzuki M
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.007815-0
  • year: 2009
  • mesh: Actinomycetales/*classification/genetics/*isolation & purification/metabolism, Animals, Base Composition, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Spiders/*microbiology
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
15908Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21742)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21742
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
21358Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM21742.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
29168Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2559228776041
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
81830Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID400181.1StrainInfo: A central database for resolving microbial strain identifiers