Strain identifier

BacDive ID: 12614

Type strain: Yes

Species: Microlunatus lacustris

Strain Designation: EL-17A, EL-17AT

Strain history: CIP <- 2001, P. Hirsch, Kiel Univ., Kiel, Germany: strain EL-17AT <- Pittisch

NCBI tax ID(s): 88568 (species)

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General

@ref: 4377

BacDive-ID: 12614

DSM-Number: 11465

keywords: 16S sequence, Bacteria, obligate aerobe, psychrophilic, Gram-positive, ovoid-shaped

description: Microlunatus lacustris EL-17A is an obligate aerobe, psychrophilic, Gram-positive bacterium that was isolated from water sample.

NCBI tax id

  • NCBI tax id: 88568
  • Matching level: species

strain history

@refhistory
4377<- M. Labrenz <- P. Hirsch; EL-17A
67770CIP 106992 <-- P. Hirsch EL-17A.
120544CIP <- 2001, P. Hirsch, Kiel Univ., Kiel, Germany: strain EL-17AT <- Pittisch

doi: 10.13145/bacdive12614.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Propionibacteriales
  • family: Propionibacteriaceae
  • genus: Microlunatus
  • species: Microlunatus lacustris
  • full scientific name: Microlunatus lacustris (Lawson et al. 2000) Nouioui et al. 2018

  • synonyms

    • @ref: 20215
    • synonym: Friedmanniella lacustris

@ref: 4377

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Propionibacteriaceae

genus: Microlunatus

species: Microlunatus lacustris

full scientific name: Microlunatus lacustris (Lawson et al. 2000) Nouioui et al. 2018

strain designation: EL-17A, EL-17AT

type strain: yes

Morphology

cell morphology

  • @ref: 120544
  • gram stain: positive
  • cell shape: ovoid-shaped
  • motility: no

colony morphology

@refcolony colorincubation periodmedium used
18360Bright red orange (2008)10-14 daysISP 2
18360Pastel yellow (1034)10-14 daysISP 3
1836010-14 daysISP 4
1836010-14 daysISP 5
18360Bright red orange (2008)10-14 daysISP 6
1836010-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium name
18360noISP 2
18360noISP 3
18360noISP 4
18360noISP 5
18360noISP 6
18360noISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4377EKHO LAKE STRAINS MEDIUM (DSMZ Medium 621a)yeshttps://mediadive.dsmz.de/medium/621aName: EKHO LAKE STRAINS MEDIUM (DSMZ Medium 621a) Composition: NaCl 22.6553 g/l Agar 15.0 g/l MgCl2 x 6 H2O 4.80666 g/l Na2SO4 3.77991 g/l CaCl2 1.06343 g/l KCl 0.64076 g/l MgSO4 x 7 H2O 0.625264 g/l Glucose 0.25 g/l Peptone 0.25 g/l Yeast extract 0.25 g/l Nitrilotriacetic acid 0.210526 g/l NaHCO3 0.18528 g/l CaCl2 x 2 H2O 0.0703158 g/l H3BO3 0.02509 g/l SrCl2 0.02316 g/l KBr 0.00579 g/l NaF 0.002895 g/l FeSO4 x 7 H2O 0.00208422 g/l Na2MoO4 x 2 H2O 0.000266736 g/l Pyridoxine hydrochloride 0.0001 g/l D-Calcium pantothenate 5e-05 g/l Nicotinamide 5e-05 g/l Thiamine-HCl x 2 H2O 5e-05 g/l Riboflavine 5e-05 g/l p-Aminobenzoic acid 5e-05 g/l Folic acid 2e-05 g/l Biotin 2e-05 g/l Vitamin B12 1e-06 g/l Metall salt sol. 44 Distilled water
18360ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18360ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18360ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18360ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18360ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18360ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
33033MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
120544CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
4377positivegrowth20psychrophilic
18360positiveoptimum20psychrophilic
33033positivegrowth20psychrophilic
67770positivegrowth25mesophilic
120544positivegrowth10-37
120544nogrowth41thermophilic
120544nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 120544
  • oxygen tolerance: obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
120544NaClpositivegrowth0-4 %
120544NaClnogrowth6 %
120544NaClnogrowth8 %
120544NaClnogrowth10 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H4)

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371arbutin-builds acid from18305
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371D-arabinose-builds acid from17108
68371glycerol-builds acid from17754
120544citrate-carbon source16947
120544esculin+hydrolysis4853
120544hippurate+hydrolysis606565
120544nitrate+reduction17632
120544nitrite-reduction16301
120544nitrate-respiration17632
68379nitrate-reduction17632
68379esculin+hydrolysis4853
68379urea-hydrolysis16199
68379gelatin-hydrolysis5291
68379D-glucose-fermentation17634
68379D-ribose+fermentation16988
68379D-xylose+fermentation65327
68379D-mannitol-fermentation16899
68379maltose-fermentation17306
68379lactose-fermentation17716
68379sucrose+fermentation17992
68379glycogen-fermentation28087

antibiotic resistance

  • @ref: 120544
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 120544
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12054415688acetoin-
12054417234glucose-

enzymes

@refvalueactivityec
68379gelatinase-
68379urease-3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase+3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase+3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase-3.5.1.B15
120544oxidase+
120544beta-galactosidase-3.2.1.23
120544alcohol dehydrogenase-1.1.1.1
120544gelatinase-
120544amylase+
120544DNase+
120544caseinase-3.4.21.50
120544catalase+1.11.1.6
120544tween esterase-
120544gamma-glutamyltransferase+2.3.2.2
120544lysine decarboxylase-4.1.1.18
120544ornithine decarboxylase-4.1.1.17
120544phenylalanine ammonia-lyase-4.3.1.24
120544tryptophan deaminase-
120544urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase lipase (C 8)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase+3.2.1.24

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
18360--+++++++---++---+-

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
18360+-+-+++++++++-+++++
120544++++++---++++-++++--

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
120544-+/--+/-+/-+/-+/---+/-+/-+/-+/-+/-+/--------+/--+/-----+/--------+/-------+/-+/----

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120544++++++++++++++++++++++++-++++--+++++++-+++-----------------++----------------------------++--------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcontinentcountryorigin.countryisolation date
4377water sampleEkho LakeAustralia and Oceania
67770Water sample of the hypersaline Ekho LakeVestfold HillsAntarcticaAntarcticaATA
120544Environment, WaterEkho lakeAntarcticaAntarcticaATA1990

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic

taxonmaps

  • @ref: 69479
  • File name: preview.99_21828.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_72;96_5617;97_13378;98_16466;99_21828&stattab=map
  • Last taxonomy: Friedmanniella luteola
  • 16S sequence: AJ132943
  • Sequence Identity:
  • Total samples: 879
  • soil counts: 432
  • aquatic counts: 81
  • animal counts: 347
  • plant counts: 19

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
183601
43771Risk group (German classification)
1205441Risk group (French classification)

Sequence information

16S sequences

  • @ref: 4377
  • description: Friedmanniella sp. EL-17a 16S rRNA gene, partial
  • accession: AJ132943
  • length: 1476
  • database: ena
  • NCBI tax ID: 88568

GC content

@refGC-contentmethod
437773
6777073high performance liquid chromatography (HPLC)

External links

@ref: 4377

culture collection no.: DSM 11465, ATCC BAA 165, NCFB 3066, JCM 11951, CIP 106992, KACC 15021, NCIMB 703066

straininfo link

  • @ref: 81828
  • straininfo: 44308

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny10930074New LL-diaminopimelic acid-containing actinomycetes from hypersaline, heliothermal and meromictic Antarctic Ekho Lake: Nocardioides aquaticus sp. nov. and Friedmanniella [correction of Friedmannielly] lacustris sp. nov.Lawson PA, Collins MD, Schumann P, Tindall BJ, Hirsch P, Labrenz MSyst Appl Microbiol10.1016/s0723-2020(00)80008-92000Actinomycetales/*classification, Antarctic Regions, Bacterial Typing Techniques, DNA, Ribosomal/genetics, Diaminopimelic Acid/*isolation & purification, Molecular Sequence Data, RNA, Ribosomal, 16S/genetics, *Water MicrobiologyGenetics
Phylogeny26873462Friedmanniella aerolata sp. nov., isolated from air.Kim SJ, Hamada M, Ahn JH, Weon HY, Suzuki KI, Kwon SWInt J Syst Evol Microbiol10.1099/ijsem.0.0009732016Actinomycetales/*classification/genetics/isolation & purification, *Air Microbiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, Vitamin K 2/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitle
4377Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 11465)https://www.dsmz.de/collection/catalogue/details/culture/DSM-11465
18360Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM11465.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
33033Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/19183
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
81828Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID44308.1StrainInfo: A central database for resolving microbial strain identifiers
120544Curators of the CIPCollection of Institut Pasteur (CIP 106992)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106992