Strain identifier

BacDive ID: 12603

Type strain: Yes

Species: Promicromonospora aerolata

Strain Designation: V-54A, V54A

Strain history: CIP <- 2003, CCM <- H.J. Busse, Vienna Univ., Vienna, Austria <- C. Zlamala: strain V54A

NCBI tax ID(s): 195749 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 6200

BacDive-ID: 12603

DSM-Number: 15943

keywords: 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-positive, rod-shaped

description: Promicromonospora aerolata V-54A is an obligate aerobe, mesophilic, Gram-positive bacterium that was isolated from air in the medieval "Virgilkapelle".

NCBI tax id

  • NCBI tax id: 195749
  • Matching level: species

strain history

@refhistory
6200<- P. Kämpfer <- H. J. Busse <- C. Zlamala
67770CCM 7043 <-- H.-J. Busse V54A <-- C. Zlamala.
116069CIP <- 2003, CCM <- H.J. Busse, Vienna Univ., Vienna, Austria <- C. Zlamala: strain V54A

doi: 10.13145/bacdive12603.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Promicromonosporaceae
  • genus: Promicromonospora
  • species: Promicromonospora aerolata
  • full scientific name: Promicromonospora aerolata Busse et al. 2003

@ref: 6200

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Promicromonosporaceae

genus: Promicromonospora

species: Promicromonospora aerolata

full scientific name: Promicromonospora aerolata Busse et al. 2003

strain designation: V-54A, V54A

type strain: yes

Morphology

cell morphology

  • @ref: 116069
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 116069

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
33207MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
6200GPHF-MEDIUM (DSMZ Medium 553)yesName: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled waterhttps://mediadive.dsmz.de/medium/553
6200GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yesName: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/65
116069CIP Medium 456yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=456
116069CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
33207positivegrowth25mesophilic
67770positivegrowth28mesophilic
6200positivegrowth28mesophilic
116069positivegrowth10-30
116069nogrowth37mesophilic
116069nogrowth41thermophilic
116069nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 116069
  • oxygen tolerance: obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
116069NaClpositivegrowth0-6 %
116069NaClnogrowth8 %
116069NaClnogrowth10 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11606916947citrate-carbon source
1160694853esculin-hydrolysis
116069606565hippurate-hydrolysis
11606917632nitrate-reduction
11606916301nitrite-reduction
11606917632nitrate-respiration

antibiotic resistance

  • @ref: 116069
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 116069
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11606915688acetoin-
11606917234glucose-

enzymes

@refvalueactivityec
116069oxidase-
116069beta-galactosidase+3.2.1.23
116069alcohol dehydrogenase-1.1.1.1
116069gelatinase+/-
116069amylase+
116069DNase+
116069caseinase+3.4.21.50
116069catalase+1.11.1.6
116069tween esterase-
116069gamma-glutamyltransferase+2.3.2.2
116069lysine decarboxylase-4.1.1.18
116069ornithine decarboxylase-4.1.1.17
116069phenylalanine ammonia-lyase-4.3.1.24
116069tryptophan deaminase-
116069urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116069-+++-+-----+++-++++-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116069+++++--+-++++--++++++++++-+----+-+++-+-++--+----++----++++-++--------------+-+-++-----+-+++----+---

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
6200air in the medieval "Virgilkapelle"ViennaAustriaAUTEurope
67770Air in the 'Virgilkapelle' chapelViennaAustriaAUTEurope
116069Environment, Air in the chapelViennaAustriaAUTEurope1999

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Air
  • Cat3: #Indoor Air

taxonmaps

  • @ref: 69479
  • File name: preview.99_69468.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_9;96_1939;97_2331;98_2876;99_69468&stattab=map
  • Last taxonomy: Promicromonospora
  • 16S sequence: AJ487303
  • Sequence Identity:
  • Total samples: 8
  • soil counts: 5
  • animal counts: 1
  • plant counts: 2

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
62001Risk group (German classification)
1160691Risk group (French classification)

Sequence information

16S sequences

  • @ref: 6200
  • description: Promicromonospora aerolata partial 16S rRNA gene, type strain V-54AT
  • accession: AJ487303
  • length: 1393
  • database: ena
  • NCBI tax ID: 195749

GC content

@refGC-contentmethod
620070
6777070high performance liquid chromatography (HPLC)

External links

@ref: 6200

culture collection no.: DSM 15943, CCM 7043, IFO 16526, NBRC 16526, JCM 14119, CIP 108163

straininfo link

  • @ref: 81817
  • straininfo: 88108

literature

  • topic: Phylogeny
  • Pubmed-ID: 13130039
  • title: Promicromonospora vindobonensis sp. nov. and Promicromonospora aerolata sp. nov., isolated from the air in the medieval 'Virgilkapelle' in Vienna.
  • authors: Busse HJ, Zlamala C, Buczolits S, Lubitz W, Kampfer P, Takeuchi M
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.02522-0
  • year: 2003
  • mesh: Actinobacteria/*classification/genetics/*isolation & purification/metabolism, Air Microbiology, Austria, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/analysis, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Species Specificity
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitle
6200Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15943)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15943
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
33207Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5755
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
81817Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID88108.1StrainInfo: A central database for resolving microbial strain identifiers
116069Curators of the CIPCollection of Institut Pasteur (CIP 108163)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108163