Strain identifier
BacDive ID: 12603
Type strain:
Species: Promicromonospora aerolata
Strain Designation: V-54A, V54A
Strain history: CIP <- 2003, CCM <- H.J. Busse, Vienna Univ., Vienna, Austria <- C. Zlamala: strain V54A
NCBI tax ID(s): 195749 (species)
General
@ref: 6200
BacDive-ID: 12603
DSM-Number: 15943
keywords: 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-positive, rod-shaped
description: Promicromonospora aerolata V-54A is an obligate aerobe, mesophilic, Gram-positive bacterium that was isolated from air in the medieval "Virgilkapelle".
NCBI tax id
- NCBI tax id: 195749
- Matching level: species
strain history
@ref | history |
---|---|
6200 | <- P. Kämpfer <- H. J. Busse <- C. Zlamala |
67770 | CCM 7043 <-- H.-J. Busse V54A <-- C. Zlamala. |
116069 | CIP <- 2003, CCM <- H.J. Busse, Vienna Univ., Vienna, Austria <- C. Zlamala: strain V54A |
doi: 10.13145/bacdive12603.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Micrococcales
- family: Promicromonosporaceae
- genus: Promicromonospora
- species: Promicromonospora aerolata
- full scientific name: Promicromonospora aerolata Busse et al. 2003
@ref: 6200
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Promicromonosporaceae
genus: Promicromonospora
species: Promicromonospora aerolata
full scientific name: Promicromonospora aerolata Busse et al. 2003
strain designation: V-54A, V54A
type strain: yes
Morphology
cell morphology
- @ref: 116069
- gram stain: positive
- cell shape: rod-shaped
- motility: no
colony morphology
- @ref: 116069
Culture and growth conditions
culture medium
@ref | name | growth | composition | link |
---|---|---|---|---|
33207 | MEDIUM 72- for trypto casein soja agar | yes | Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g) | |
6200 | GPHF-MEDIUM (DSMZ Medium 553) | yes | Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water | https://mediadive.dsmz.de/medium/553 |
6200 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | yes | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water | https://mediadive.dsmz.de/medium/65 |
116069 | CIP Medium 456 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=456 | |
116069 | CIP Medium 72 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
33207 | positive | growth | 25 | mesophilic |
67770 | positive | growth | 28 | mesophilic |
6200 | positive | growth | 28 | mesophilic |
116069 | positive | growth | 10-30 | |
116069 | no | growth | 37 | mesophilic |
116069 | no | growth | 41 | thermophilic |
116069 | no | growth | 45 | thermophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 116069
- oxygen tolerance: obligate aerobe
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
116069 | NaCl | positive | growth | 0-6 % |
116069 | NaCl | no | growth | 8 % |
116069 | NaCl | no | growth | 10 % |
observation
- @ref: 67770
- observation: quinones: MK-9(H4)
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
116069 | 16947 | citrate | - | carbon source |
116069 | 4853 | esculin | - | hydrolysis |
116069 | 606565 | hippurate | - | hydrolysis |
116069 | 17632 | nitrate | - | reduction |
116069 | 16301 | nitrite | - | reduction |
116069 | 17632 | nitrate | - | respiration |
antibiotic resistance
- @ref: 116069
- metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
- is antibiotic: yes
- is sensitive: no
- is resistant: yes
metabolite production
- @ref: 116069
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test | methylred-test |
---|---|---|---|---|
116069 | 15688 | acetoin | - | |
116069 | 17234 | glucose | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
116069 | oxidase | - | |
116069 | beta-galactosidase | + | 3.2.1.23 |
116069 | alcohol dehydrogenase | - | 1.1.1.1 |
116069 | gelatinase | +/- | |
116069 | amylase | + | |
116069 | DNase | + | |
116069 | caseinase | + | 3.4.21.50 |
116069 | catalase | + | 1.11.1.6 |
116069 | tween esterase | - | |
116069 | gamma-glutamyltransferase | + | 2.3.2.2 |
116069 | lysine decarboxylase | - | 4.1.1.18 |
116069 | ornithine decarboxylase | - | 4.1.1.17 |
116069 | phenylalanine ammonia-lyase | - | 4.3.1.24 |
116069 | tryptophan deaminase | - | |
116069 | urease | - | 3.5.1.5 |
68382 | alkaline phosphatase | + | 3.1.3.1 |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | + | |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | - | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | - | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | - | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | alpha-galactosidase | + | 3.2.1.22 |
68382 | beta-galactosidase | + | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | + | 3.2.1.20 |
68382 | beta-glucosidase | + | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
68382 | alpha-mannosidase | + | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
116069 | - | + | + | + | - | + | - | - | - | - | - | + | + | + | - | + | + | + | + | - |
API biotype100
@ref | GLU | FRU | GAL | TRE | MNE | SBE | MEL | SAC | RAF | MTE | MAL | LAC | LTE | MbGa | MaGa | CEL | GEN | MbGu | ESC | RIB | ARA | XYL | PLE | RHA | FUC | MLZ | DARL | LARL | XLT | DUL | TAG | GLY | INO | MAN | MTL | TUR | SOR | ADO | HBG | LYX | ERY | MDG | 3MDG | SAT | MUC | LTAT | DTAT | MTAT | DMLT | LMLT | CATE | TATE | TTE | CIT | GRT | GAT | 2KG | 5KG | TRY | NAG | GNT | PAC | PAT | pOBE | QAT | GTE | mOBE | BAT | PPAT | CMT | TGE | BET | PCE | ABT | HIN | LAT | CAP | CYT | HIS | SUC | FUM | GRE | GYT | AVT | ETN | TTN | GLN | ITA | 3OBU | APT | GTT | PRO | DALA | LALA | SER | MNT | PROP | TYR | 2KT |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
116069 | + | + | + | + | + | - | - | + | - | + | + | + | + | - | - | + | + | + | + | + | + | + | + | + | + | - | + | - | - | - | - | + | - | + | + | + | - | + | - | + | + | - | - | + | - | - | - | - | + | + | - | - | - | - | + | + | + | + | - | + | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | + | - | + | + | - | - | - | - | - | + | - | + | + | + | - | - | - | - | + | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | isolation date |
---|---|---|---|---|---|---|
6200 | air in the medieval "Virgilkapelle" | Vienna | Austria | AUT | Europe | |
67770 | Air in the 'Virgilkapelle' chapel | Vienna | Austria | AUT | Europe | |
116069 | Environment, Air in the chapel | Vienna | Austria | AUT | Europe | 1999 |
isolation source categories
- Cat1: #Environmental
- Cat2: #Air
- Cat3: #Indoor Air
taxonmaps
- @ref: 69479
- File name: preview.99_69468.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_9;96_1939;97_2331;98_2876;99_69468&stattab=map
- Last taxonomy: Promicromonospora
- 16S sequence: AJ487303
- Sequence Identity:
- Total samples: 8
- soil counts: 5
- animal counts: 1
- plant counts: 2
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
6200 | 1 | Risk group (German classification) |
116069 | 1 | Risk group (French classification) |
Sequence information
16S sequences
- @ref: 6200
- description: Promicromonospora aerolata partial 16S rRNA gene, type strain V-54AT
- accession: AJ487303
- length: 1393
- database: ena
- NCBI tax ID: 195749
GC content
@ref | GC-content | method |
---|---|---|
6200 | 70 | |
67770 | 70 | high performance liquid chromatography (HPLC) |
External links
@ref: 6200
culture collection no.: DSM 15943, CCM 7043, IFO 16526, NBRC 16526, JCM 14119, CIP 108163
straininfo link
- @ref: 81817
- straininfo: 88108
literature
- topic: Phylogeny
- Pubmed-ID: 13130039
- title: Promicromonospora vindobonensis sp. nov. and Promicromonospora aerolata sp. nov., isolated from the air in the medieval 'Virgilkapelle' in Vienna.
- authors: Busse HJ, Zlamala C, Buczolits S, Lubitz W, Kampfer P, Takeuchi M
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.02522-0
- year: 2003
- mesh: Actinobacteria/*classification/genetics/*isolation & purification/metabolism, Air Microbiology, Austria, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/analysis, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Species Specificity
- topic2: Genetics
Reference
@id | authors | catalogue | doi/url | title |
---|---|---|---|---|
6200 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15943) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-15943 | |
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
33207 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/5755 | ||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||
68382 | Automatically annotated from API zym | |||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |
81817 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID88108.1 | StrainInfo: A central database for resolving microbial strain identifiers | |
116069 | Curators of the CIP | Collection of Institut Pasteur (CIP 108163) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108163 |