Strain identifier
BacDive ID: 12587
Type strain: 
Species: Isoptericola nanjingensis
Strain Designation: H17
Strain history: DSM 24300 <-- H. Zhi H17.
NCBI tax ID(s): 903413 (species)
version 8.1 (current version)
General
@ref: 17625
BacDive-ID: 12587
DSM-Number: 24300
keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, ovoid-shaped
description: Isoptericola nanjingensis H17 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from soil.
NCBI tax id
- NCBI tax id: 903413
- Matching level: species
strain history
| @ref | history |
|---|---|
| 17625 | <- H. Zhi, Nanjing Agric. Univ., College of Life Science, China; H17 |
| 67770 | DSM 24300 <-- H. Zhi H17. |
doi: 10.13145/bacdive12587.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Micrococcales
- family: Promicromonosporaceae
- genus: Isoptericola
- species: Isoptericola nanjingensis
- full scientific name: Isoptericola nanjingensis Huang et al. 2012
@ref: 17625
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Promicromonosporaceae
genus: Isoptericola
species: Isoptericola nanjingensis
full scientific name: Isoptericola nanjingensis Huang et al. 2012
strain designation: H17
type strain: yes
Morphology
cell morphology
- @ref: 30304
- gram stain: positive
- cell shape: ovoid-shaped
- motility: no
pigmentation
- @ref: 30304
- production: yes
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 17625 | TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) | yes | https://mediadive.dsmz.de/medium/92 | Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water |
| 17625 | TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) | yes | https://mediadive.dsmz.de/medium/535 | Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water |
| 17625 | BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) | yes | https://mediadive.dsmz.de/medium/514 | Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water |
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 17625 | positive | growth | 28 | mesophilic |
| 30304 | positive | optimum | 30 | mesophilic |
| 67770 | positive | growth | 28 | mesophilic |
culture pH
- @ref: 30304
- ability: positive
- type: optimum
- pH: 7
Physiology and metabolism
oxygen tolerance
- @ref: 30304
- oxygen tolerance: aerobe
spore formation
- @ref: 30304
- spore formation: no
halophily
- @ref: 30304
- salt: NaCl
- growth: positive
- tested relation: optimum
- concentration: 3 %
murein
- @ref: 17625
- murein short key: A11.31
- type: A4alpha L-Lys-D-Asp
observation
- @ref: 67770
- observation: quinones: MK-9(H4)
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 30304 | 18403 | L-arabitol | + | carbon source |
| 30304 | 17716 | lactose | + | carbon source |
| 30304 | 29864 | mannitol | + | carbon source |
| 30304 | 28053 | melibiose | + | carbon source |
| 30304 | 16634 | raffinose | + | carbon source |
| 30304 | 26546 | rhamnose | + | carbon source |
| 30304 | 33942 | ribose | + | carbon source |
| 30304 | 27082 | trehalose | + | carbon source |
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 30304 | alkaline phosphatase | + | 3.1.3.1 |
| 30304 | alpha-galactosidase | + | 3.2.1.22 |
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent | latitude | longitude |
|---|---|---|---|---|---|---|---|
| 17625 | soil | Jiangning, Nanjing, Houhan Road | China | CHN | Asia | 31.7833 | 118.733 |
| 67770 | Soil | Nanjing, Jiangsu | China | CHN | Asia |
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Soil
taxonmaps
- @ref: 69479
- File name: preview.99_3403.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_9;96_1778;97_2128;98_2608;99_3403&stattab=map
- Last taxonomy: Isoptericola
- 16S sequence: HQ222356
- Sequence Identity:
- Total samples: 1061
- soil counts: 472
- aquatic counts: 100
- animal counts: 365
- plant counts: 124
Safety information
risk assessment
- @ref: 17625
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 17625
- description: Isoptericola nanjingensis strain H17 16S ribosomal RNA gene, partial sequence
- accession: HQ222356
- length: 1404
- database: ena
- NCBI tax ID: 903413
GC content
| @ref | GC-content | method |
|---|---|---|
| 17625 | 72.4 | thermal denaturation, midpoint method (Tm) |
| 30304 | 72.4 |
External links
@ref: 17625
culture collection no.: DSM 24300, CCTCC AB 2011005, JCM 19549
straininfo link
- @ref: 81801
- straininfo: 400588
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 21669920 | Isoptericola nanjingensis sp. nov., a mineral-weathering bacterium. | Huang Z, Sheng XF, Zhao F, He LY, Huang J, Wang Q | Int J Syst Evol Microbiol | 10.1099/ijs.0.033043-0 | 2011 | Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistry | Genetics |
| Phylogeny | 27045419 | Isoptericola cucumis sp. nov., isolated from the root tissue of cucumber (Cucumis sativus). | Kampfer P, Glaeser SP, Kloepper JW, Hu CH, McInroy JA, Martin K, Busse HJ | Int J Syst Evol Microbiol | 10.1099/ijsem.0.001055 | 2016 | Actinobacteria/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Cucumis sativus/*microbiology, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, Plant Roots/*microbiology, Polyamines/chemistry, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry | Transcriptome |
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
|---|---|---|---|---|---|---|
| 17625 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 24300) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-24300 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 30304 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 26645 | 28776041 | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 81801 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID400588.1 | StrainInfo: A central database for resolving microbial strain identifiers |