Strain identifier

BacDive ID: 12565

Type strain: Yes

Species: Prevotella dentasini

Strain history: M. Hirasawa NUM 1903.

NCBI tax ID(s): 1236509 (strain), 589537 (species)

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General

@ref: 16216

BacDive-ID: 12565

DSM-Number: 22229

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-negative, rod-shaped

description: Prevotella dentasini DSM 22229 is an anaerobe, mesophilic, Gram-negative bacterium that was isolated from oral cavity of a donkey.

NCBI tax id

NCBI tax idMatching level
589537species
1236509strain

strain history

@refhistory
16216<- M. Hirasawa, Nihon Univ. School; NUM 1903
67770M. Hirasawa NUM 1903.

doi: 10.13145/bacdive12565.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Bacteroidia
  • order: Bacteroidales
  • family: Prevotellaceae
  • genus: Prevotella
  • species: Prevotella dentasini
  • full scientific name: Prevotella dentasini Takada et al. 2010

@ref: 16216

domain: Bacteria

phylum: Bacteroidetes

class: Bacteroidia

order: Bacteroidales

family: Prevotellaceae

genus: Prevotella

species: Prevotella dentasini

full scientific name: Prevotella dentasini Takada et al. 2010

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
29614negative1.5-2.5 µm0.6-0.8 µmrod-shapedno
68367rod-shaped
68367negative
69480negative99.997

pigmentation

  • @ref: 29614
  • production: yes

Culture and growth conditions

culture medium

  • @ref: 16216
  • name: COLUMBIA BLOOD AGAR (DSMZ Medium 429)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/429
  • composition: Name: COLUMBIA BLOOD AGAR (DSMZ Medium 429) Composition: Horse blood 40.0 g/l Columbia agar base

culture temp

@refgrowthtypetemperaturerange
16216positivegrowth37mesophilic
29614positivegrowth37mesophilic
29614positiveoptimum37mesophilic
67770positivegrowth37mesophilic

culture pH

@refabilitytypepH
29614positivegrowth7
29614positiveoptimum7

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
16216anaerobe
29614anaerobe
69480anaerobe99.626

spore formation

@refspore formationconfidence
29614no
68367no
69481no100
69480no100

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2961417234glucose+carbon source
2961417754glycerol+carbon source
2961417306maltose+carbon source
2961416634raffinose+carbon source
2961417992sucrose+carbon source
6836727082trehalose-builds acid from
6836762345L-rhamnose-builds acid from
6836730911sorbitol-builds acid from
6836716634raffinose-builds acid from
683676731melezitose-builds acid from
6836716024D-mannose-builds acid from
6836717057cellobiose-builds acid from
6836717754glycerol-builds acid from
683674853esculin-hydrolysis
683675291gelatin-hydrolysis
6836730849L-arabinose-builds acid from
6836765327D-xylose-builds acid from
6836717814salicin-builds acid from
6836717306maltose-builds acid from
6836717992sucrose-builds acid from
6836717716lactose-builds acid from
6836716899D-mannitol-builds acid from
6836717634D-glucose-builds acid from
6836716199urea-hydrolysis
6836727897tryptophan+energy source
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan+energy source

metabolite production

@refChebi-IDmetaboliteproduction
6836735581indoleyes
6838035581indoleyes

metabolite tests

@refChebi-IDmetaboliteindole test
6838035581indole+
6836735581indole+

enzymes

@refvalueactivityec
29614acid phosphatase+3.1.3.2
29614alkaline phosphatase+3.1.3.1
29614gelatinase+
68380serine arylamidase-
68380glutamyl-glutamate arylamidase+
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase+3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase+3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase-
68380alkaline phosphatase+3.1.3.1
68380tryptophan deaminase+4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase-3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase-3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5
68367catalase-1.11.1.6
68367beta-glucosidase-3.2.1.21
68367gelatinase-
68367urease-3.5.1.5

API 20A

@refINDUREGLUMANLACSACMALSALXYLARAGELESCGLYCELMNEMLZRAFSORRHATRECATSPORGRAMCOCC
16216+-----------------------

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
16216---------------++--+----+--+-

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
16216oral cavity of a donkeyJapanJPNAsia
67770Oral cavity of donkeys

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Equidae (Horse)
#Host Body-Site#Oral cavity and airways

taxonmaps

  • @ref: 69479
  • File name: preview.99_3512.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_44;96_1825;97_2193;98_2692;99_3512&stattab=map
  • Last taxonomy: Prevotella dentasini subclade
  • 16S sequence: AB477014
  • Sequence Identity:
  • Total samples: 18702
  • soil counts: 87
  • aquatic counts: 80
  • animal counts: 18481
  • plant counts: 54

Safety information

risk assessment

  • @ref: 16216
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Prevotella dentasini gene for 16S ribosomal RNA, partial sequence, strain: JCM 15908AB5476811494ena1236509
16216Prevotella dentasini gene for 16S ribosomal RNA, partial sequenceAB4770141525ena1236509

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Prevotella dentasini JCM 159081236509.3wgspatric1236509
66792Prevotella dentasini JCM 159082585427895draftimg1236509
67770Prevotella dentasini JCM 15908GCA_000614065contigncbi1236509

GC content

@refGC-contentmethod
1621650.2high performance liquid chromatography (HPLC)
2961450.2

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno92.17yes
gram-positiveno97.83yes
anaerobicyes98.46yes
aerobicno97.768no
halophileno86.449no
spore-formingno97.447no
glucose-utilyes84.185yes
flagellatedno96.485yes
thermophileno98.306yes
glucose-fermentyes58.014no

External links

@ref: 16216

culture collection no.: DSM 22229, JCM 15908, NUM 1903, KCTC 15187

straininfo link

  • @ref: 81779
  • straininfo: 396765

literature

  • topic: Phylogeny
  • Pubmed-ID: 19717577
  • title: Prevotella dentasini sp. nov., a black-pigmented species isolated from the oral cavity of donkeys.
  • authors: Takada K, Hayashi K, Sato Y, Hirasawa M
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.017020-0
  • year: 2009
  • mesh: Animals, Base Sequence, Equidae/*microbiology, Molecular Sequence Data, Mouth/*microbiology, Phylogeny, Prevotella/*classification/genetics/isolation & purification/physiology
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
16216Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22229)https://www.dsmz.de/collection/catalogue/details/culture/DSM-22229
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
29614Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2877604126004
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68367Automatically annotated from API 20A
68380Automatically annotated from API rID32A
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
81779Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID396765.1StrainInfo: A central database for resolving microbial strain identifiers