Strain identifier
BacDive ID: 12563
Type strain:
Species: Prevotella falsenii
Strain Designation: 04052
Strain history: M. Sakamoto 04052 <-- H. Kumada Pi 04052.
NCBI tax ID(s): 1122983 (strain), 515414 (species)
General
@ref: 16580
BacDive-ID: 12563
DSM-Number: 22864
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-negative, rod-shaped, animal pathogen
description: Prevotella falsenii 04052 is an anaerobe, mesophilic, Gram-negative animal pathogen that was isolated from monkey dental plaque .
NCBI tax id
NCBI tax id | Matching level |
---|---|
515414 | species |
1122983 | strain |
strain history
@ref | history |
---|---|
16580 | <- CCUG <- M. Sakamoto, JCM/RIKEN <- H. Kumada, Kanagawa Dental College, Yokosuka, Japan; 04052 |
67770 | M. Sakamoto 04052 <-- H. Kumada Pi 04052. |
doi: 10.13145/bacdive12563.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacteroidota
- domain: Bacteria
- phylum: Bacteroidota
- class: Bacteroidia
- order: Bacteroidales
- family: Prevotellaceae
- genus: Prevotella
- species: Prevotella falsenii
- full scientific name: Prevotella falsenii Sakamoto et al. 2009
@ref: 16580
domain: Bacteria
phylum: Bacteroidetes
class: Bacteroidia
order: Bacteroidales
family: Prevotellaceae
genus: Prevotella
species: Prevotella falsenii
full scientific name: Prevotella falsenii Sakamoto et al. 2009
strain designation: 04052
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility | confidence |
---|---|---|---|---|---|---|
28959 | negative | 1.6 µm | 0.65 µm | rod-shaped | no | |
68367 | rod-shaped | |||||
68367 | negative | |||||
69480 | negative | 99.999 |
colony morphology
@ref | incubation period |
---|---|
16580 | 1-2 days |
60873 | 2 days |
pigmentation
- @ref: 28959
- production: yes
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
16580 | COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) | yes | https://mediadive.dsmz.de/medium/693 | Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base |
16580 | PYG MEDIUM (MODIFIED) (DSMZ Medium 104) | yes | https://mediadive.dsmz.de/medium/104 | Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
16580 | positive | growth | 37 | mesophilic |
60873 | positive | growth | 37 | mesophilic |
67770 | positive | growth | 37 | mesophilic |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance | confidence |
---|---|---|
16580 | anaerobe | |
28959 | anaerobe | |
60873 | anaerobe | |
69480 | anaerobe | 99.999 |
spore formation
@ref | spore formation | confidence |
---|---|---|
28959 | no | |
68367 | no | |
69481 | no | 100 |
69480 | no | 99.998 |
observation
- @ref: 28959
- observation: aggregates in clumps
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
28959 | 17234 | glucose | + | carbon source |
28959 | 17306 | maltose | + | carbon source |
28959 | 37684 | mannose | + | carbon source |
28959 | 16634 | raffinose | + | carbon source |
28959 | 17992 | sucrose | + | carbon source |
68367 | 27082 | trehalose | - | builds acid from |
68367 | 62345 | L-rhamnose | - | builds acid from |
68367 | 30911 | sorbitol | - | builds acid from |
68367 | 16634 | raffinose | + | builds acid from |
68367 | 6731 | melezitose | - | builds acid from |
68367 | 16024 | D-mannose | + | builds acid from |
68367 | 17057 | cellobiose | - | builds acid from |
68367 | 17754 | glycerol | - | builds acid from |
68367 | 4853 | esculin | - | hydrolysis |
68367 | 5291 | gelatin | - | hydrolysis |
68367 | 30849 | L-arabinose | - | builds acid from |
68367 | 65327 | D-xylose | - | builds acid from |
68367 | 17814 | salicin | - | builds acid from |
68367 | 17306 | maltose | + | builds acid from |
68367 | 17992 | sucrose | + | builds acid from |
68367 | 17716 | lactose | - | builds acid from |
68367 | 16899 | D-mannitol | - | builds acid from |
68367 | 17634 | D-glucose | + | builds acid from |
68367 | 16199 | urea | - | hydrolysis |
68367 | 27897 | tryptophan | + | energy source |
68380 | 16199 | urea | - | hydrolysis |
68380 | 29016 | arginine | - | hydrolysis |
68380 | 16024 | D-mannose | - | fermentation |
68380 | 29985 | L-glutamate | - | degradation |
68380 | 17632 | nitrate | - | reduction |
68380 | 27897 | tryptophan | + | energy source |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
28959 | 35581 | indole | yes |
68367 | 35581 | indole | yes |
68380 | 35581 | indole | yes |
metabolite tests
@ref | Chebi-ID | metabolite | indole test |
---|---|---|---|
28959 | 35581 | indole | + |
68380 | 35581 | indole | + |
68367 | 35581 | indole | + |
enzymes
@ref | value | activity | ec |
---|---|---|---|
16580 | catalase | - | 1.11.1.6 |
16580 | cytochrome-c oxidase | - | 1.9.3.1 |
28959 | alkaline phosphatase | + | 3.1.3.1 |
28959 | gelatinase | + | |
68380 | serine arylamidase | - | |
68380 | glutamyl-glutamate arylamidase | + | |
68380 | histidine arylamidase | - | |
68380 | glycin arylamidase | - | |
68380 | alanine arylamidase | + | 3.4.11.2 |
68380 | tyrosine arylamidase | - | |
68380 | pyrrolidonyl arylamidase | - | 3.4.19.3 |
68380 | leucine arylamidase | - | 3.4.11.1 |
68380 | phenylalanine arylamidase | - | |
68380 | leucyl glycin arylamidase | - | 3.4.11.1 |
68380 | proline-arylamidase | - | 3.4.11.5 |
68380 | L-arginine arylamidase | + | |
68380 | alkaline phosphatase | + | 3.1.3.1 |
68380 | tryptophan deaminase | + | 4.1.99.1 |
68380 | alpha-fucosidase | + | 3.2.1.51 |
68380 | glutamate decarboxylase | - | 4.1.1.15 |
68380 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68380 | beta-glucuronidase | - | 3.2.1.31 |
68380 | alpha-arabinosidase | - | 3.2.1.55 |
68380 | beta-glucosidase | - | 3.2.1.21 |
68380 | alpha-glucosidase | + | 3.2.1.20 |
68380 | beta-Galactosidase 6-phosphate | - | |
68380 | beta-galactosidase | - | 3.2.1.23 |
68380 | alpha-galactosidase | - | 3.2.1.22 |
68380 | arginine dihydrolase | - | 3.5.3.6 |
68380 | urease | - | 3.5.1.5 |
68367 | catalase | - | 1.11.1.6 |
68367 | beta-glucosidase | - | 3.2.1.21 |
68367 | gelatinase | - | |
68367 | urease | - | 3.5.1.5 |
API 20A
@ref | IND | URE | GLU | MAN | LAC | SAC | MAL | SAL | XYL | ARA | GEL | ESC | GLY | CEL | MNE | MLZ | RAF | SOR | RHA | TRE | CAT | SPOR | GRAM | COCC |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
16580 | + | - | + | - | - | + | + | - | - | - | - | - | - | - | + | - | + | - | - | - | - | - | - | - |
API rID32A
@ref | URE | ADH Arg | alpha GAL | beta GAL | beta GP | alpha GLU | beta GLU | alpha ARA | beta GUR | beta NAG | MNE | RAF | GDC | alpha FUC | NIT | IND | PAL | ArgA | ProA | LGA | PheA | LeuA | PyrA | TyrA | AlaA | GlyA | HisA | GGA | SerA |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
16580 | - | - | - | - | - | + | - | - | - | - | - | +/- | - | + | - | + | + | + | - | - | - | - | - | - | + | - | - | + | - |
16580 | - | - | - | - | - | + | - | - | - | - | - | +/- | - | + | - | + | + | + | - | - | - | - | - | - | + | - | - | + | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | country | origin.country | continent | sampling date |
---|---|---|---|---|---|
16580 | monkey dental plaque (male long-tailed macaques, 5–6 years old) | Japan | JPN | Asia | |
60873 | Monkey dental plaque | Japan | JPN | Asia | 2004-03-01 |
67770 | Monkey dental plaque |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Host | #Mammals | #Primates |
#Host Body-Site | #Oral cavity and airways | #Plaque |
#Host Body-Site | #Oral cavity and airways | #Tooth |
taxonmaps
- @ref: 69479
- File name: preview.99_6795.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_295;96_3206;97_3935;98_5009;99_6795&stattab=map
- Last taxonomy: Prevotella falsenii subclade
- 16S sequence: AB429504
- Sequence Identity:
- Total samples: 3109
- soil counts: 21
- aquatic counts: 17
- animal counts: 3061
- plant counts: 10
Safety information
risk assessment
- @ref: 16580
- pathogenicity animal: yes, in single cases
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 16580
- description: Prevotella falsenii gene for 16S ribosomal RNA, partial sequence, strain: 04052 (= JCM 15124)
- accession: AB429504
- length: 1494
- database: ena
- NCBI tax ID: 515414
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Prevotella falsenii JCM 15124 | 1236505.3 | wgs | patric | 1122983 |
66792 | Prevotella falsenii JCM 15124 | 2585427898 | draft | img | 1122983 |
67770 | Prevotella falsenii DSM 22864 = JCM 15124 | GCA_000613945 | contig | ncbi | 1122983 |
GC content
@ref | GC-content | method |
---|---|---|
16580 | 45.2 | |
67770 | 45.2 | high performance liquid chromatography (HPLC) |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 100 | no |
motile | no | 93.585 | yes |
gram-positive | no | 97.663 | yes |
anaerobic | yes | 98.245 | yes |
aerobic | no | 98.319 | yes |
halophile | no | 87.053 | no |
spore-forming | no | 97.006 | yes |
glucose-util | yes | 85.366 | yes |
flagellated | no | 97.237 | yes |
thermophile | no | 98.229 | no |
glucose-ferment | no | 50 | no |
External links
@ref: 16580
culture collection no.: DSM 22864, CCUG 56137, JCM 15124, KCTC 15074
straininfo link
- @ref: 81777
- straininfo: 397205
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 19196771 | Prevotella falsenii sp. nov., a Prevotella intermedia-like organism isolated from monkey dental plaque. | Sakamoto M, Kumada H, Hamada N, Takahashi Y, Okamoto M, Bakir MA, Benno Y | Int J Syst Evol Microbiol | 10.1099/ijs.0.002626-0 | 2009 | Animals, Dental Plaque/*microbiology, Fatty Acids/analysis, Macaca fascicularis/*microbiology, Molecular Sequence Data, Phylogeny, Prevotella/chemistry/*classification/genetics, RNA, Ribosomal, 16S/genetics, Species Specificity | Genetics |
Phylogeny | 19654360 | Prevotella aurantiaca sp. nov., isolated from the human oral cavity. | Sakamoto M, Suzuki N, Okamoto M | Int J Syst Evol Microbiol | 10.1099/ijs.0.012831-0 | 2009 | DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/chemistry/metabolism, Humans, Molecular Sequence Data, Mouth/*microbiology, Periodontitis/microbiology, Phylogeny, Prevotella/*classification/genetics/*isolation & purification/metabolism, RNA, Ribosomal, 16S/genetics | Metabolism |
Reference
@id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
---|---|---|---|---|---|---|
16580 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22864) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-22864 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
28959 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 25396 | 28776041 | |
60873 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 56137) | https://www.ccug.se/strain?id=56137 | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68367 | Automatically annotated from API 20A | |||||
68380 | Automatically annotated from API rID32A | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
81777 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID397205.1 | StrainInfo: A central database for resolving microbial strain identifiers |