Strain identifier

BacDive ID: 12563

Type strain: Yes

Species: Prevotella falsenii

Strain Designation: 04052

Strain history: M. Sakamoto 04052 <-- H. Kumada Pi 04052.

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 16580

BacDive-ID: 12563

DSM-Number: 22864

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-negative, rod-shaped, animal pathogen

description: Prevotella falsenii 04052 is an anaerobe, mesophilic, Gram-negative animal pathogen that was isolated from monkey dental plaque .

NCBI tax id

NCBI tax idMatching level
515414species
1122983strain

strain history

@refhistory
16580<- CCUG <- M. Sakamoto, JCM/RIKEN <- H. Kumada, Kanagawa Dental College, Yokosuka, Japan; 04052
67770M. Sakamoto 04052 <-- H. Kumada Pi 04052.

doi: 10.13145/bacdive12563.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Bacteroidia
  • order: Bacteroidales
  • family: Prevotellaceae
  • genus: Prevotella
  • species: Prevotella falsenii
  • full scientific name: Prevotella falsenii Sakamoto et al. 2009

@ref: 16580

domain: Bacteria

phylum: Bacteroidetes

class: Bacteroidia

order: Bacteroidales

family: Prevotellaceae

genus: Prevotella

species: Prevotella falsenii

full scientific name: Prevotella falsenii Sakamoto et al. 2009

strain designation: 04052

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
28959negative1.6 µm0.65 µmrod-shapedno
68367rod-shaped
68367negative
69480negative99.999

colony morphology

@refincubation period
165801-2 days
608732 days

pigmentation

  • @ref: 28959
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16580COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
16580PYG MEDIUM (MODIFIED) (DSMZ Medium 104)yeshttps://mediadive.dsmz.de/medium/104Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water

culture temp

@refgrowthtypetemperaturerange
16580positivegrowth37mesophilic
60873positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
16580anaerobe
28959anaerobe
60873anaerobe
69480anaerobe99.999

spore formation

@refspore formationconfidence
28959no
68367no
69481no100
69480no99.998

observation

  • @ref: 28959
  • observation: aggregates in clumps

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2895917234glucose+carbon source
2895917306maltose+carbon source
2895937684mannose+carbon source
2895916634raffinose+carbon source
2895917992sucrose+carbon source
6836727082trehalose-builds acid from
6836762345L-rhamnose-builds acid from
6836730911sorbitol-builds acid from
6836716634raffinose+builds acid from
683676731melezitose-builds acid from
6836716024D-mannose+builds acid from
6836717057cellobiose-builds acid from
6836717754glycerol-builds acid from
683674853esculin-hydrolysis
683675291gelatin-hydrolysis
6836730849L-arabinose-builds acid from
6836765327D-xylose-builds acid from
6836717814salicin-builds acid from
6836717306maltose+builds acid from
6836717992sucrose+builds acid from
6836717716lactose-builds acid from
6836716899D-mannitol-builds acid from
6836717634D-glucose+builds acid from
6836716199urea-hydrolysis
6836727897tryptophan+energy source
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan+energy source

metabolite production

@refChebi-IDmetaboliteproduction
2895935581indoleyes
6836735581indoleyes
6838035581indoleyes

metabolite tests

@refChebi-IDmetaboliteindole test
2895935581indole+
6838035581indole+
6836735581indole+

enzymes

@refvalueactivityec
16580catalase-1.11.1.6
16580cytochrome-c oxidase-1.9.3.1
28959alkaline phosphatase+3.1.3.1
28959gelatinase+
68380serine arylamidase-
68380glutamyl-glutamate arylamidase+
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase+3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase+
68380alkaline phosphatase+3.1.3.1
68380tryptophan deaminase+4.1.99.1
68380alpha-fucosidase+3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase+3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase-3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5
68367catalase-1.11.1.6
68367beta-glucosidase-3.2.1.21
68367gelatinase-
68367urease-3.5.1.5

API 20A

@refINDUREGLUMANLACSACMALSALXYLARAGELESCGLYCELMNEMLZRAFSORRHATRECATSPORGRAMCOCC
16580+-+--++-------+-+-------

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
16580-----+-----+/--+-+++------+--+-
16580-----+-----+/--+-+++------+--+-

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentsampling date
16580monkey dental plaque (male long-tailed macaques, 5–6 years old)JapanJPNAsia
60873Monkey dental plaqueJapanJPNAsia2004-03-01
67770Monkey dental plaque

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Primates
#Host Body-Site#Oral cavity and airways#Plaque
#Host Body-Site#Oral cavity and airways#Tooth

taxonmaps

  • @ref: 69479
  • File name: preview.99_6795.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_295;96_3206;97_3935;98_5009;99_6795&stattab=map
  • Last taxonomy: Prevotella falsenii subclade
  • 16S sequence: AB429504
  • Sequence Identity:
  • Total samples: 3109
  • soil counts: 21
  • aquatic counts: 17
  • animal counts: 3061
  • plant counts: 10

Safety information

risk assessment

  • @ref: 16580
  • pathogenicity animal: yes, in single cases
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 16580
  • description: Prevotella falsenii gene for 16S ribosomal RNA, partial sequence, strain: 04052 (= JCM 15124)
  • accession: AB429504
  • length: 1494
  • database: ena
  • NCBI tax ID: 515414

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Prevotella falsenii JCM 151241236505.3wgspatric1122983
66792Prevotella falsenii JCM 151242585427898draftimg1122983
67770Prevotella falsenii DSM 22864 = JCM 15124GCA_000613945contigncbi1122983

GC content

@refGC-contentmethod
1658045.2
6777045.2high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno93.585yes
gram-positiveno97.663yes
anaerobicyes98.245yes
aerobicno98.319yes
halophileno87.053no
spore-formingno97.006yes
glucose-utilyes85.366yes
flagellatedno97.237yes
thermophileno98.229no
glucose-fermentno50no

External links

@ref: 16580

culture collection no.: DSM 22864, CCUG 56137, JCM 15124, KCTC 15074

straininfo link

  • @ref: 81777
  • straininfo: 397205

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19196771Prevotella falsenii sp. nov., a Prevotella intermedia-like organism isolated from monkey dental plaque.Sakamoto M, Kumada H, Hamada N, Takahashi Y, Okamoto M, Bakir MA, Benno YInt J Syst Evol Microbiol10.1099/ijs.0.002626-02009Animals, Dental Plaque/*microbiology, Fatty Acids/analysis, Macaca fascicularis/*microbiology, Molecular Sequence Data, Phylogeny, Prevotella/chemistry/*classification/genetics, RNA, Ribosomal, 16S/genetics, Species SpecificityGenetics
Phylogeny19654360Prevotella aurantiaca sp. nov., isolated from the human oral cavity.Sakamoto M, Suzuki N, Okamoto MInt J Syst Evol Microbiol10.1099/ijs.0.012831-02009DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/chemistry/metabolism, Humans, Molecular Sequence Data, Mouth/*microbiology, Periodontitis/microbiology, Phylogeny, Prevotella/*classification/genetics/*isolation & purification/metabolism, RNA, Ribosomal, 16S/geneticsMetabolism

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
16580Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22864)https://www.dsmz.de/collection/catalogue/details/culture/DSM-22864
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
28959Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2539628776041
60873Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 56137)https://www.ccug.se/strain?id=56137
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68367Automatically annotated from API 20A
68380Automatically annotated from API rID32A
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
81777Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID397205.1StrainInfo: A central database for resolving microbial strain identifiers