Strain identifier

BacDive ID: 12557

Type strain: Yes

Species: Segatella maculosa

Strain Designation: W1609

Strain history: CCUG 54766 <-- J. Downes <-- W. G. Wade W1609.

NCBI tax ID(s): 1122986 (strain), 439703 (species)

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General

@ref: 8056

BacDive-ID: 12557

DSM-Number: 19339

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-negative, rod-shaped, human pathogen

description: Segatella maculosa W1609 is an anaerobe, mesophilic, Gram-negative human pathogen that was isolated from human oral cavity.

NCBI tax id

NCBI tax idMatching level
1122986strain
439703species

strain history

@refhistory
8056<- J. Downes; W1609
67770CCUG 54766 <-- J. Downes <-- W. G. Wade W1609.

doi: 10.13145/bacdive12557.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Bacteroidia
  • order: Bacteroidales
  • family: Prevotellaceae
  • genus: Segatella
  • species: Segatella maculosa
  • full scientific name: Segatella maculosa (Downes et al. 2007) Hitch et al. 2023

  • synonyms

    • @ref: 20215
    • synonym: Prevotella maculosa

@ref: 8056

domain: Bacteria

phylum: Bacteroidetes

class: Bacteroidia

order: Bacteroidales

family: Prevotellaceae

genus: Prevotella

species: Prevotella maculosa

full scientific name: Prevotella maculosa Downes et al. 2007

strain designation: W1609

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
32300negative2 µm0.7 µmrod-shapedno
69480negative99.997

colony morphology

  • @ref: 60318
  • incubation period: 2 days

pigmentation

  • @ref: 32300
  • production: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8056COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
8056PYG MEDIUM (MODIFIED) (DSMZ Medium 104)yeshttps://mediadive.dsmz.de/medium/104Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water

culture temp

@refgrowthtypetemperaturerange
8056positivegrowth37mesophilic
60318positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
8056anaerobe
32300anaerobe
60318anaerobe
69480anaerobe99.999

spore formation

@refspore formationconfidence
69481no100
69480no99.998

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3230030089acetate+carbon source
3230022599arabinose+carbon source
3230017057cellobiose+carbon source
3230028757fructose+carbon source
3230017234glucose+carbon source
3230017716lactose+carbon source
3230017306maltose+carbon source
3230037684mannose+carbon source
3230028053melibiose+carbon source
3230016634raffinose+carbon source
3230026546rhamnose+carbon source
3230017814salicin+carbon source
3230030031succinate+carbon source
3230017992sucrose+carbon source
3230018222xylose+carbon source
323004853esculin+hydrolysis
6836727082trehalose-builds acid from
6836762345L-rhamnose-builds acid from
6836730911sorbitol-builds acid from
6836716634raffinose-builds acid from
683676731melezitose-builds acid from
6836716024D-mannose-builds acid from
6836717057cellobiose-builds acid from
6836717754glycerol-builds acid from
683674853esculin-hydrolysis
683675291gelatin-hydrolysis
6836730849L-arabinose-builds acid from
6836765327D-xylose-builds acid from
6836717814salicin+builds acid from
6836717306maltose-builds acid from
6836717992sucrose-builds acid from
6836717716lactose-builds acid from
6836716899D-mannitol-builds acid from
6836717634D-glucose-builds acid from
6836716199urea-hydrolysis
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
32300alkaline phosphatase+3.1.3.1
32300alpha-galactosidase+3.2.1.22
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase+3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase+3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase-
68380alkaline phosphatase+3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase+3.2.1.52
68380beta-glucuronidase+3.2.1.31
68380alpha-arabinosidase+3.2.1.55
68380beta-glucosidase+3.2.1.21
68380alpha-glucosidase+3.2.1.20
68380beta-galactosidase+3.2.1.23
68380alpha-galactosidase+3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5
68367beta-glucosidase-3.2.1.21
68367gelatinase-
68367urease-3.5.1.5

API 20A

@refUREGLUMANLACSACMALSALXYLARAGELESCGLYCELMNEMLZRAFSORRHATRE
8056------+------------

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
8056--+++/-+++++------+--+----+----

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
8056human oral cavityCardiffUnited KingdomGBREurope
60318Human oral cavity,pericoronitisCardiffUnited KingdomGBREurope1989
67770Human oral cavity

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body-Site#Oral cavity and airways
#Host Body-Site#Oral cavity and airways#Mouth

taxonmaps

  • @ref: 69479
  • File name: preview.99_3637.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_44;96_1886;97_2265;98_2780;99_3637&stattab=map
  • Last taxonomy: Prevotella maculosa subclade
  • 16S sequence: AB547690
  • Sequence Identity:
  • Total samples: 17153
  • soil counts: 188
  • aquatic counts: 232
  • animal counts: 16676
  • plant counts: 57

Safety information

risk assessment

  • @ref: 8056
  • pathogenicity human: yes, in single cases
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
8056Prevotella maculosa strain W1609 16S ribosomal RNA gene, partial sequenceEF5343141454ena439703
8056Prevotella maculosa strain W1609 16S ribosomal RNA gene, partial sequenceEF5343151454ena439703
67770Prevotella maculosa gene for 16S ribosomal RNA, partial sequence, strain: JCM 15638AB5476901405ena439703

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Prevotella maculosa DSM 193391122986.3wgspatric1122986
66792Prevotella maculosa JCM 156381236506.4wgspatric1122986
66792Prevotella maculosa JCM 156382585427901draftimg1122986
66792Prevotella maculosa DSM 193392521172638draftimg1122986
67770Prevotella maculosa DSM 19339 = JCM 15638GCA_000382385scaffoldncbi1122986
67770Prevotella maculosa DSM 19339 = JCM 15638GCA_000613965contigncbi1122986

GC content

@refGC-contentmethod
805648
6777048high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno89.827yes
flagellatedno97.387yes
gram-positiveno97.274no
anaerobicyes98.892yes
aerobicno97.383yes
halophileno91.141no
spore-formingno96.101no
glucose-utilyes85.32no
thermophileno90.758yes
glucose-fermentyes68.523no

External links

@ref: 8056

culture collection no.: DSM 19339, CCUG 54766, JCM 15638

straininfo link

  • @ref: 81771
  • straininfo: 402457

literature

  • topic: Phylogeny
  • Pubmed-ID: 18048753
  • title: Prevotella maculosa sp. nov., isolated from the human oral cavity.
  • authors: Downes J, Sutcliffe IC, Booth V, Wade WG
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.65281-0
  • year: 2007
  • mesh: Acetic Acid/metabolism, Anaerobiosis/physiology, Bacterial Typing Techniques, Base Composition, Carbohydrate Metabolism, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Genes, rRNA, Humans, Molecular Sequence Data, Mouth/*microbiology, Phylogeny, Prevotella/*classification/genetics/*isolation & purification/metabolism, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Succinic Acid/metabolism
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
8056Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19339)https://www.dsmz.de/collection/catalogue/details/culture/DSM-19339
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
32300Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2853928776041
60318Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 54766)https://www.ccug.se/strain?id=54766
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68367Automatically annotated from API 20A
68380Automatically annotated from API rID32A
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
81771Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID402457.1StrainInfo: A central database for resolving microbial strain identifiers