Strain identifier

BacDive ID: 12553

Type strain: Yes

Species: Hoylesella loescheii

Strain history: Tokyo Med. Dent. Univ. <-- Tama Biochem. Co., Ltd. <-- ATCC 15930 <-- W. J. Loesche 8B (Bacteroides oralis).

NCBI tax ID(s): 1122985 (strain), 840 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 8242

BacDive-ID: 12553

DSM-Number: 19665

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic

description: Hoylesella loescheii DSM 19665 is an anaerobe, mesophilic bacterium that was isolated from gingival crevice.

NCBI tax id

NCBI tax idMatching level
840species
1122985strain

strain history

@refhistory
8242<- JCM/RIKEN; Loesche strain 8B {2007} <- CCUG <- ATCC <- W. J. Loesche
67770Tokyo Med. Dent. Univ. <-- Tama Biochem. Co., Ltd. <-- ATCC 15930 <-- W. J. Loesche 8B (Bacteroides oralis).

doi: 10.13145/bacdive12553.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Bacteroidia
  • order: Bacteroidales
  • family: Prevotellaceae
  • genus: Hoylesella
  • species: Hoylesella loescheii
  • full scientific name: Hoylesella loescheii (Holdeman and Johnson 1982) Hitch et al. 2023
  • synonyms

    @refsynonym
    20215Prevotella loescheii
    20215Bacteroides loescheii

@ref: 8242

domain: Bacteria

phylum: Bacteroidetes

class: Bacteroidia

order: Bacteroidales

family: Prevotellaceae

genus: Prevotella

species: Prevotella loescheii

full scientific name: Prevotella loescheii (Holdeman and Johnson 1982) Shah and Collins 1990 emend. Wu et al. 1992 emend. Hahnke et al. 2016

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram stain
69480no93.975
6948099.997negative

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8242COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
8242PYG MEDIUM (MODIFIED) (DSMZ Medium 104)yeshttps://mediadive.dsmz.de/medium/104Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water

culture temp

@refgrowthtypetemperaturerange
8242positivegrowth37mesophilic
44876positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
8242anaerobe
44876anaerobe
69480anaerobe98.651

spore formation

@refspore formationconfidence
69481no100
69480no99.996

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose+fermentation
6838016634raffinose+fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68380serine arylamidase-
68380glutamyl-glutamate arylamidase+
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase+3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase+3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase-
68380alkaline phosphatase+3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase+3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase+3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase+3.2.1.21
68380alpha-glucosidase+3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase+3.2.1.23
68380alpha-galactosidase+3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
8242--++-++--+++-+--+--+----+--+-

Isolation, sampling and environmental information

isolation

@refsample type
8242gingival crevice
44876Gingival crevice
67770Gingival crevice

isolation source categories

  • Cat1: #Host Body-Site
  • Cat2: #Oral cavity and airways
  • Cat3: #Gingiva

taxonmaps

  • @ref: 69479
  • File name: preview.99_1175.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_198;96_687;97_798;98_925;99_1175&stattab=map
  • Last taxonomy: Prevotella loescheii subclade
  • 16S sequence: AB547689
  • Sequence Identity:
  • Total samples: 27850
  • soil counts: 377
  • aquatic counts: 404
  • animal counts: 26967
  • plant counts: 102

Safety information

risk assessment

  • @ref: 8242
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Prevotella loescheii gene for 16S ribosomal RNA, partial sequence, strain: JCM 12249AB5476881490ena840
20218Prevotella loescheii strain NCTC 11321 16S ribosomal RNA gene, partial sequenceAY8365081451ena840
8242Prevotella loescheii ATCC 15930 16S ribosomal RNA gene, complete sequenceL164811480ena840
67770Prevotella loescheii gene for 16S ribosomal RNA, partial sequence, strain: JCM 8530AB5476891476ena840

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Prevotella loescheii DSM 19665 = JCM 12249 = ATCC 159301122985.7wgspatric1122985
66792Prevotella loescheii DSM 19665 = JCM 12249 = ATCC 159301122985.3wgspatric1122985
66792Prevotella loescheii DSM 19665 = JCM 12249 = ATCC 159301122985.6wgspatric1122985
66792Prevotella loescheii 8B, DSM 196652518645604draftimg1122985
67770Hoylesella loescheii DSM 19665 = JCM 12249 = ATCC 15930GCA_000378085scaffoldncbi1122985
67770Hoylesella loescheii DSM 19665 = JCM 12249 = ATCC 15930GCA_000613485contigncbi1122985
67770Hoylesella loescheii DSM 19665 = JCM 12249 = ATCC 15930GCA_000698925scaffoldncbi1122985

GC content

  • @ref: 8242
  • GC-content: 46.6
  • method: sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno92.497no
flagellatedno96.634no
gram-positiveno97.139no
anaerobicyes98.167yes
aerobicno98.077no
halophileno84.396no
spore-formingno95.723no
glucose-utilyes83.117no
thermophileno99.072yes
glucose-fermentyes57.488no

External links

@ref: 8242

culture collection no.: DSM 19665, ATCC 15930, JCM 12249, NCTC 11321, CCUG 5914, Loesche strain 8B, JCM 8530, BCRC 10621, VPI 9085

straininfo link

  • @ref: 81768
  • straininfo: 92360

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Pathogenicity1408357Suppressive effect of soluble factor(s) derived from Prevotella loescheii ATCC 15930 on proliferation of human lymphocytes.Terao N, Saito S, Hayakawa M, Abiko YOral Microbiol Immunol10.1111/j.1399-302x.1992.tb00030.x1992Bacterial Proteins/*immunology, Bacteroides/*immunology/pathogenicity, Cell Division/drug effects, DNA/biosynthesis, Humans, Immunologic Factors/*immunology, *Lymphocyte Activation, Lymphocytes/drug effects/*immunology, Precursor Cell Lymphoblastic Leukemia-Lymphoma, Sonication, Tumor Cells, Cultured, Virulence
Enzymology1820573Purification and properties of neuraminidase from the culture medium of Bacteroides loescheii.Takeshita T, Takagaki M, Amano A, Murakami Y, Nagata H, Shizukuishi SOral Microbiol Immunol10.1111/j.1399-302x.1991.tb00501.x1991Bacteroides/*enzymology, Chromatography, Neuraminidase/chemistry/*isolation & purificationPhylogeny
Enzymology2488923[Purification and some properties of neuraminidase from the culture medium of Bacteroides loescheii ATCC 15930].Takeshita TOsaka Daigaku Shigaku Zasshi1989Bacteroides/*enzymology, Culture Media, Hemagglutination/*immunology, Neuraminidase/*metabolismCultivation
Phylogeny6735473Immunoglobulin G response to subgingival gram-negative bacteria in human subjects.Naito Y, Okuda K, Takazoe IInfect Immun10.1128/iai.45.1.47-51.19841984Adult, *Antibody Formation, Bacteroides/immunology, Enzyme-Linked Immunosorbent Assay, Fusobacterium/immunology, Gingiva/*microbiology, Gram-Negative Bacteria/*immunology/isolation & purification, Humans, Immunoglobulin G/*analysis, Periodontitis/*microbiology, Reference Values, Species SpecificityPathogenicity
Pathogenicity7560233Effect of periodontal treatments on serum IgG antibody titers against periodontopathic bacteria.Horibe M, Watanabe H, Ishikawa IJ Clin Periodontol10.1111/j.1600-051x.1995.tb00798.x1995Adult, Aggregatibacter actinomycetemcomitans/immunology, Alveolar Bone Loss/microbiology/surgery/therapy, Antibodies, Bacterial/*blood, Bacteria/*immunology, Capnocytophaga/immunology, Dental Scaling, Eikenella corrodens/immunology, Female, Fusobacterium nucleatum/immunology, Humans, Immunoglobulin G/*blood, Male, Middle Aged, Periodontal Pocket/microbiology/surgery/therapy, Periodontitis/*microbiology/surgery/*therapy, Porphyromonas gingivalis/immunology, Prevotella/immunology, Prevotella intermedia/immunology, Root Planing
Metabolism9345789Fermentation product analysis in the identification of black-pigmented bacteria from periodontitis.Takada K, Hirasawa MMicrobios1997Antibodies, Bacterial/analysis/immunology, Antigens, Bacterial/analysis/immunology, Bacteriological Techniques, Chromatography, Gas, Culture Media/*analysis/metabolism, Electrophoresis, Polyacrylamide Gel, Fatty Acids/*analysis/metabolism, Fermentation, Gram-Negative Anaerobic Bacteria/growth & development/immunology/*metabolism, Humans, Immunodiffusion, Periodontitis/*microbiologyEnzymology
Enzymology11389867Isolation and characterisation of dipeptidyl peptidase IV from Prevotella loescheii ATCC 15930.Koreeda Y, Hayakawa M, Ikemi T, Abiko YArch Oral Biol10.1016/s0003-9969(00)00065-02001Alanine/metabolism, Cadmium/pharmacology, Chromatography, Chromatography, Gel, Chromatography, High Pressure Liquid, Cobalt/pharmacology, Coumarins, Dipeptides, Dipeptidyl Peptidase 4/chemistry/drug effects/*isolation & purification, Electrophoresis, Polyacrylamide Gel, Enzyme Inhibitors/pharmacology, Fluorescent Dyes, Humans, Hydrogen-Ion Concentration, Isoelectric Point, Isoflurophate/pharmacology, Mercaptoethanol, Mercury/pharmacology, Molecular Weight, Prevotella/classification/*enzymology, Proline/metabolism, Protease Inhibitors/pharmacology, Serine Proteinase Inhibitors/pharmacology, Zinc/pharmacologyPhylogeny
Metabolism11408950Inhibitory effect of lactoperoxidase-generated hypothiocyanite upon black pigmented anaerobe growth.Fadel M, Courtois PInt J Mol Med2001Analysis of Variance, Cell Division/drug effects, Glucose Oxidase/metabolism/pharmacology, Hydrogen Peroxide/metabolism, Hydrogen-Ion Concentration, Lactoperoxidase/metabolism/*pharmacology, Porphyromonas gingivalis/*drug effects/growth & development/metabolism, Prevotella/*drug effects/growth & development/metabolism, Prevotella melaninogenica/drug effects/growth & development/metabolism, Thiocyanates/metabolism/*pharmacology, Time FactorsPathogenicity
Phylogeny15143039Prevotella shahii sp. nov. and Prevotella salivae sp. nov., isolated from the human oral cavity.Sakamoto M, Suzuki M, Huang Y, Umeda M, Ishikawa I, Benno YInt J Syst Evol Microbiol10.1099/ijs.0.02876-02004DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/analysis, Humans, Molecular Sequence Data, Mouth/*microbiology, Phenotype, Phylogeny, Prevotella/*classification/genetics/*isolation & purification, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Species SpecificityPathogenicity

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8242Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19665)https://www.dsmz.de/collection/catalogue/details/culture/DSM-19665
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
44876Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 5914)https://www.ccug.se/strain?id=5914
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68380Automatically annotated from API rID32A
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
81768Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID92360.1StrainInfo: A central database for resolving microbial strain identifiers