Strain identifier
BacDive ID: 12553
Type strain:
Species: Hoylesella loescheii
Strain history: Tokyo Med. Dent. Univ. <-- Tama Biochem. Co., Ltd. <-- ATCC 15930 <-- W. J. Loesche 8B (Bacteroides oralis).
NCBI tax ID(s): 1122985 (strain), 840 (species)
General
@ref: 8242
BacDive-ID: 12553
DSM-Number: 19665
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic
description: Hoylesella loescheii DSM 19665 is an anaerobe, mesophilic bacterium that was isolated from gingival crevice.
NCBI tax id
NCBI tax id | Matching level |
---|---|
840 | species |
1122985 | strain |
strain history
@ref | history |
---|---|
8242 | <- JCM/RIKEN; Loesche strain 8B {2007} <- CCUG <- ATCC <- W. J. Loesche |
67770 | Tokyo Med. Dent. Univ. <-- Tama Biochem. Co., Ltd. <-- ATCC 15930 <-- W. J. Loesche 8B (Bacteroides oralis). |
doi: 10.13145/bacdive12553.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacteroidota
- domain: Bacteria
- phylum: Bacteroidota
- class: Bacteroidia
- order: Bacteroidales
- family: Prevotellaceae
- genus: Hoylesella
- species: Hoylesella loescheii
- full scientific name: Hoylesella loescheii (Holdeman and Johnson 1982) Hitch et al. 2023
synonyms
@ref synonym 20215 Prevotella loescheii 20215 Bacteroides loescheii
@ref: 8242
domain: Bacteria
phylum: Bacteroidetes
class: Bacteroidia
order: Bacteroidales
family: Prevotellaceae
genus: Prevotella
species: Prevotella loescheii
full scientific name: Prevotella loescheii (Holdeman and Johnson 1982) Shah and Collins 1990 emend. Wu et al. 1992 emend. Hahnke et al. 2016
type strain: yes
Morphology
cell morphology
@ref | motility | confidence | gram stain |
---|---|---|---|
69480 | no | 93.975 | |
69480 | 99.997 | negative |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
8242 | COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) | yes | https://mediadive.dsmz.de/medium/693 | Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base |
8242 | PYG MEDIUM (MODIFIED) (DSMZ Medium 104) | yes | https://mediadive.dsmz.de/medium/104 | Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
8242 | positive | growth | 37 | mesophilic |
44876 | positive | growth | 37 | mesophilic |
67770 | positive | growth | 37 | mesophilic |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance | confidence |
---|---|---|
8242 | anaerobe | |
44876 | anaerobe | |
69480 | anaerobe | 98.651 |
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | no | 100 |
69480 | no | 99.996 |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
68380 | 16199 | urea | - | hydrolysis |
68380 | 29016 | arginine | - | hydrolysis |
68380 | 16024 | D-mannose | + | fermentation |
68380 | 16634 | raffinose | + | fermentation |
68380 | 29985 | L-glutamate | - | degradation |
68380 | 17632 | nitrate | - | reduction |
68380 | 27897 | tryptophan | - | energy source |
metabolite production
- @ref: 68380
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 68380
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
68380 | serine arylamidase | - | |
68380 | glutamyl-glutamate arylamidase | + | |
68380 | histidine arylamidase | - | |
68380 | glycin arylamidase | - | |
68380 | alanine arylamidase | + | 3.4.11.2 |
68380 | tyrosine arylamidase | - | |
68380 | pyrrolidonyl arylamidase | - | 3.4.19.3 |
68380 | leucine arylamidase | - | 3.4.11.1 |
68380 | phenylalanine arylamidase | - | |
68380 | leucyl glycin arylamidase | + | 3.4.11.1 |
68380 | proline-arylamidase | - | 3.4.11.5 |
68380 | L-arginine arylamidase | - | |
68380 | alkaline phosphatase | + | 3.1.3.1 |
68380 | tryptophan deaminase | - | 4.1.99.1 |
68380 | alpha-fucosidase | + | 3.2.1.51 |
68380 | glutamate decarboxylase | - | 4.1.1.15 |
68380 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
68380 | beta-glucuronidase | - | 3.2.1.31 |
68380 | alpha-arabinosidase | - | 3.2.1.55 |
68380 | beta-glucosidase | + | 3.2.1.21 |
68380 | alpha-glucosidase | + | 3.2.1.20 |
68380 | beta-Galactosidase 6-phosphate | - | |
68380 | beta-galactosidase | + | 3.2.1.23 |
68380 | alpha-galactosidase | + | 3.2.1.22 |
68380 | arginine dihydrolase | - | 3.5.3.6 |
68380 | urease | - | 3.5.1.5 |
API rID32A
@ref | URE | ADH Arg | alpha GAL | beta GAL | beta GP | alpha GLU | beta GLU | alpha ARA | beta GUR | beta NAG | MNE | RAF | GDC | alpha FUC | NIT | IND | PAL | ArgA | ProA | LGA | PheA | LeuA | PyrA | TyrA | AlaA | GlyA | HisA | GGA | SerA |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
8242 | - | - | + | + | - | + | + | - | - | + | + | + | - | + | - | - | + | - | - | + | - | - | - | - | + | - | - | + | - |
Isolation, sampling and environmental information
isolation
@ref | sample type |
---|---|
8242 | gingival crevice |
44876 | Gingival crevice |
67770 | Gingival crevice |
isolation source categories
- Cat1: #Host Body-Site
- Cat2: #Oral cavity and airways
- Cat3: #Gingiva
taxonmaps
- @ref: 69479
- File name: preview.99_1175.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_198;96_687;97_798;98_925;99_1175&stattab=map
- Last taxonomy: Prevotella loescheii subclade
- 16S sequence: AB547689
- Sequence Identity:
- Total samples: 27850
- soil counts: 377
- aquatic counts: 404
- animal counts: 26967
- plant counts: 102
Safety information
risk assessment
- @ref: 8242
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Prevotella loescheii gene for 16S ribosomal RNA, partial sequence, strain: JCM 12249 | AB547688 | 1490 | ena | 840 |
20218 | Prevotella loescheii strain NCTC 11321 16S ribosomal RNA gene, partial sequence | AY836508 | 1451 | ena | 840 |
8242 | Prevotella loescheii ATCC 15930 16S ribosomal RNA gene, complete sequence | L16481 | 1480 | ena | 840 |
67770 | Prevotella loescheii gene for 16S ribosomal RNA, partial sequence, strain: JCM 8530 | AB547689 | 1476 | ena | 840 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Prevotella loescheii DSM 19665 = JCM 12249 = ATCC 15930 | 1122985.7 | wgs | patric | 1122985 |
66792 | Prevotella loescheii DSM 19665 = JCM 12249 = ATCC 15930 | 1122985.3 | wgs | patric | 1122985 |
66792 | Prevotella loescheii DSM 19665 = JCM 12249 = ATCC 15930 | 1122985.6 | wgs | patric | 1122985 |
66792 | Prevotella loescheii 8B, DSM 19665 | 2518645604 | draft | img | 1122985 |
67770 | Hoylesella loescheii DSM 19665 = JCM 12249 = ATCC 15930 | GCA_000378085 | scaffold | ncbi | 1122985 |
67770 | Hoylesella loescheii DSM 19665 = JCM 12249 = ATCC 15930 | GCA_000613485 | contig | ncbi | 1122985 |
67770 | Hoylesella loescheii DSM 19665 = JCM 12249 = ATCC 15930 | GCA_000698925 | scaffold | ncbi | 1122985 |
GC content
- @ref: 8242
- GC-content: 46.6
- method: sequence analysis
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 100 | no |
motile | no | 92.497 | no |
flagellated | no | 96.634 | no |
gram-positive | no | 97.139 | no |
anaerobic | yes | 98.167 | yes |
aerobic | no | 98.077 | no |
halophile | no | 84.396 | no |
spore-forming | no | 95.723 | no |
glucose-util | yes | 83.117 | no |
thermophile | no | 99.072 | yes |
glucose-ferment | yes | 57.488 | no |
External links
@ref: 8242
culture collection no.: DSM 19665, ATCC 15930, JCM 12249, NCTC 11321, CCUG 5914, Loesche strain 8B, JCM 8530, BCRC 10621, VPI 9085
straininfo link
- @ref: 81768
- straininfo: 92360
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Pathogenicity | 1408357 | Suppressive effect of soluble factor(s) derived from Prevotella loescheii ATCC 15930 on proliferation of human lymphocytes. | Terao N, Saito S, Hayakawa M, Abiko Y | Oral Microbiol Immunol | 10.1111/j.1399-302x.1992.tb00030.x | 1992 | Bacterial Proteins/*immunology, Bacteroides/*immunology/pathogenicity, Cell Division/drug effects, DNA/biosynthesis, Humans, Immunologic Factors/*immunology, *Lymphocyte Activation, Lymphocytes/drug effects/*immunology, Precursor Cell Lymphoblastic Leukemia-Lymphoma, Sonication, Tumor Cells, Cultured, Virulence | |
Enzymology | 1820573 | Purification and properties of neuraminidase from the culture medium of Bacteroides loescheii. | Takeshita T, Takagaki M, Amano A, Murakami Y, Nagata H, Shizukuishi S | Oral Microbiol Immunol | 10.1111/j.1399-302x.1991.tb00501.x | 1991 | Bacteroides/*enzymology, Chromatography, Neuraminidase/chemistry/*isolation & purification | Phylogeny |
Enzymology | 2488923 | [Purification and some properties of neuraminidase from the culture medium of Bacteroides loescheii ATCC 15930]. | Takeshita T | Osaka Daigaku Shigaku Zasshi | 1989 | Bacteroides/*enzymology, Culture Media, Hemagglutination/*immunology, Neuraminidase/*metabolism | Cultivation | |
Phylogeny | 6735473 | Immunoglobulin G response to subgingival gram-negative bacteria in human subjects. | Naito Y, Okuda K, Takazoe I | Infect Immun | 10.1128/iai.45.1.47-51.1984 | 1984 | Adult, *Antibody Formation, Bacteroides/immunology, Enzyme-Linked Immunosorbent Assay, Fusobacterium/immunology, Gingiva/*microbiology, Gram-Negative Bacteria/*immunology/isolation & purification, Humans, Immunoglobulin G/*analysis, Periodontitis/*microbiology, Reference Values, Species Specificity | Pathogenicity |
Pathogenicity | 7560233 | Effect of periodontal treatments on serum IgG antibody titers against periodontopathic bacteria. | Horibe M, Watanabe H, Ishikawa I | J Clin Periodontol | 10.1111/j.1600-051x.1995.tb00798.x | 1995 | Adult, Aggregatibacter actinomycetemcomitans/immunology, Alveolar Bone Loss/microbiology/surgery/therapy, Antibodies, Bacterial/*blood, Bacteria/*immunology, Capnocytophaga/immunology, Dental Scaling, Eikenella corrodens/immunology, Female, Fusobacterium nucleatum/immunology, Humans, Immunoglobulin G/*blood, Male, Middle Aged, Periodontal Pocket/microbiology/surgery/therapy, Periodontitis/*microbiology/surgery/*therapy, Porphyromonas gingivalis/immunology, Prevotella/immunology, Prevotella intermedia/immunology, Root Planing | |
Metabolism | 9345789 | Fermentation product analysis in the identification of black-pigmented bacteria from periodontitis. | Takada K, Hirasawa M | Microbios | 1997 | Antibodies, Bacterial/analysis/immunology, Antigens, Bacterial/analysis/immunology, Bacteriological Techniques, Chromatography, Gas, Culture Media/*analysis/metabolism, Electrophoresis, Polyacrylamide Gel, Fatty Acids/*analysis/metabolism, Fermentation, Gram-Negative Anaerobic Bacteria/growth & development/immunology/*metabolism, Humans, Immunodiffusion, Periodontitis/*microbiology | Enzymology | |
Enzymology | 11389867 | Isolation and characterisation of dipeptidyl peptidase IV from Prevotella loescheii ATCC 15930. | Koreeda Y, Hayakawa M, Ikemi T, Abiko Y | Arch Oral Biol | 10.1016/s0003-9969(00)00065-0 | 2001 | Alanine/metabolism, Cadmium/pharmacology, Chromatography, Chromatography, Gel, Chromatography, High Pressure Liquid, Cobalt/pharmacology, Coumarins, Dipeptides, Dipeptidyl Peptidase 4/chemistry/drug effects/*isolation & purification, Electrophoresis, Polyacrylamide Gel, Enzyme Inhibitors/pharmacology, Fluorescent Dyes, Humans, Hydrogen-Ion Concentration, Isoelectric Point, Isoflurophate/pharmacology, Mercaptoethanol, Mercury/pharmacology, Molecular Weight, Prevotella/classification/*enzymology, Proline/metabolism, Protease Inhibitors/pharmacology, Serine Proteinase Inhibitors/pharmacology, Zinc/pharmacology | Phylogeny |
Metabolism | 11408950 | Inhibitory effect of lactoperoxidase-generated hypothiocyanite upon black pigmented anaerobe growth. | Fadel M, Courtois P | Int J Mol Med | 2001 | Analysis of Variance, Cell Division/drug effects, Glucose Oxidase/metabolism/pharmacology, Hydrogen Peroxide/metabolism, Hydrogen-Ion Concentration, Lactoperoxidase/metabolism/*pharmacology, Porphyromonas gingivalis/*drug effects/growth & development/metabolism, Prevotella/*drug effects/growth & development/metabolism, Prevotella melaninogenica/drug effects/growth & development/metabolism, Thiocyanates/metabolism/*pharmacology, Time Factors | Pathogenicity | |
Phylogeny | 15143039 | Prevotella shahii sp. nov. and Prevotella salivae sp. nov., isolated from the human oral cavity. | Sakamoto M, Suzuki M, Huang Y, Umeda M, Ishikawa I, Benno Y | Int J Syst Evol Microbiol | 10.1099/ijs.0.02876-0 | 2004 | DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/analysis, Humans, Molecular Sequence Data, Mouth/*microbiology, Phenotype, Phylogeny, Prevotella/*classification/genetics/*isolation & purification, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Species Specificity | Pathogenicity |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
8242 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19665) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-19665 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
44876 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 5914) | https://www.ccug.se/strain?id=5914 | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68380 | Automatically annotated from API rID32A | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
81768 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID92360.1 | StrainInfo: A central database for resolving microbial strain identifiers |