Strain identifier

BacDive ID: 12539

Type strain: Yes

Species: Prevotella shahii

Strain Designation: EHS11

Strain history: M. Sakamoto EHS11.

For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 6 (current version):
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 6 (current version)

General

@ref: 5929

BacDive-ID: 12539

DSM-Number: 15611

keywords: mesophilic, anaerobe, Bacteria, 16S sequence, Gram-negative, genome sequence, rod-shaped

description: Prevotella shahii EHS11 is an anaerobe, mesophilic, Gram-negative bacterium that was isolated from human oral cavity, saliva of a peridontally healthy subject.

NCBI tax id

NCBI tax idMatching level
1122991strain
228603species

strain history

doi: 10.13145/bacdive12539.20211221.6

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidetes
  • domain: Bacteria
  • phylum: Bacteroidetes
  • class: Bacteroidia
  • order: Bacteroidales
  • family: Prevotellaceae
  • genus: Prevotella
  • species: Prevotella shahii
  • full scientific name: Prevotella shahii Sakamoto et al. 2004

@ref: 5929

domain: Bacteria

phylum: Bacteroidetes

class: Bacteroidia

order: Bacteroidales

family: Prevotellaceae

genus: Prevotella

species: Prevotella shahii

full scientific name: Prevotella shahii Sakamoto et al. 2004

strain designation: EHS11

type strain: yes

Morphology

cell morphology

  • @ref: 30045
  • gram stain: negative
  • cell length: 9.15 µm
  • cell width: 0.65 µm
  • cell shape: rod-shaped
  • motility: no

pigmentation

  • @ref: 30045
  • production: no

Culture and growth conditions

culture medium

  • @ref: 5929
  • name: PYG MEDIUM (MODIFIED) (DSMZ Medium 104)
  • growth: yes
  • link: https://bacmedia.dsmz.de/medium/104
  • composition: Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water

culture temp

@refgrowthtypetemperaturerange
5929positivegrowth37mesophilic
59371positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
5929anaerobe
30045anaerobe
59371anaerobe

spore formation

  • @ref: 30045
  • spore formation: no

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
300455291gelatin+carbon source
3004517234glucose+carbon source
3004517716lactose+carbon source
3004517306maltose+carbon source
3004537684mannose+carbon source
30045506227N-acetylglucosamine+carbon source
3004516634raffinose+carbon source
3004517992sucrose+carbon source

enzymes

@refvalueactivityec
30045acid phosphatase+3.1.3.2
30045alkaline phosphatase+3.1.3.1
30045alpha-galactosidase+3.2.1.22
30045gelatinase+

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
5929human oral cavity, saliva of a peridontally healthy subjectJapanJPNAsia
59371Human saliva,periodontally healthy subject
67770Human oral cavity

isolation source categories

Cat1Cat2
#Host#Human
#Host Body-Site#Oral cavity and airways

Safety information

risk assessment

  • @ref: 5929
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 5929
  • description: Prevotella shahii gene for 16S rRNA, complete sequence
  • accession: AB108825
  • length: 1490
  • database: ena
  • NCBI tax ID: 228603

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Prevotella shahii DSM 15611 = JCM 12083 DSM 15611GCA_003201715scaffoldncbi1122991
66792Prevotella shahii DSM 15611 = JCM 12083 JCM 12083GCA_000613445contigncbi1122991
66792Prevotella shahii DSM 15611 = JCM 120831122991.5wgspatric1122991
66792Prevotella shahii JCM 120831235817.5wgspatric1122991
66792Prevotella shahii DSM 156112585427597draftimg1122991
66792Prevotella shahii JCM 120832585427909draftimg1122991
67770Prevotella shahii DSM 15611 = JCM 12083, whole genome shotgun sequencing projectBAIZ00000000ncbi1122991
67770Prevotella shahii DSM 15611 = JCM 12083 strain DSM 15611, whole genome shotgun sequencing projectQJJX00000000ncbi1122991

GC content

@refGC-contentmethod
592944.3
6777044.3high performance liquid chromatography (HPLC)

External links

@ref: 5929

culture collection no.: DSM 15611, JCM 12083, CCUG 51933, KCTC 15078

straininfo link

@refpassport
20218http://www.straininfo.net/strains/378417
20218http://www.straininfo.net/strains/371098

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
5929Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15611)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15611
20215D.Gleim, M.Kracht, N.Weiss et. al.http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.htmlProkaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
2640610.1099/ijs.0.02876-015143039
30045Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2877604126406
59371Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 51933)https://www.ccug.se/strain?id=51933
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/