Strain identifier

BacDive ID: 12536

Type strain: Yes

Species: Segatella bryantii

Strain Designation: B14, B14

Strain history: CIP <- 1998, G. Avgustin, Univ. Ljubljana, Slovenia: strain B14 <- H.J. Flint, Rowett. Res. Inst., Aberdeen, Scotland <- J.B. Russell, Cornell Univ., Ithaca, N.Y., Bacteroides rumicola subsp. brevis

NCBI tax ID(s): 77095 (species)

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General

@ref: 4314

BacDive-ID: 12536

DSM-Number: 11371

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-negative, rod-shaped

description: Segatella bryantii B14 is an anaerobe, mesophilic, Gram-negative bacterium that was isolated from rumen of cow.

NCBI tax id

  • NCBI tax id: 77095
  • Matching level: species

strain history

@refhistory
4314<- G. Avgustin <- H.J. Flint <- J.B. Russell <- M.P. Bryant, B14 (Bacteroides ruminicola subsp. brevis)
117065CIP <- 1998, G. Avgustin, Univ. Ljubljana, Slovenia: strain B14 <- H.J. Flint, Rowett. Res. Inst., Aberdeen, Scotland <- J.B. Russell, Cornell Univ., Ithaca, N.Y., Bacteroides rumicola subsp. brevis

doi: 10.13145/bacdive12536.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Bacteroidia
  • order: Bacteroidales
  • family: Prevotellaceae
  • genus: Segatella
  • species: Segatella bryantii
  • full scientific name: Segatella bryantii (Avguštin et al. 1997) Hitch et al. 2023

  • synonyms

    • @ref: 20215
    • synonym: Prevotella bryantii

@ref: 4314

domain: Bacteria

phylum: Bacteroidetes

class: Bacteroidia

order: Bacteroidales

family: Prevotellaceae

genus: Prevotella

species: Prevotella bryantii

full scientific name: Prevotella bryantii Avguštin et al. 1997 emend. Hahnke et al. 2016

strain designation: B14, B14

type strain: yes

Morphology

cell morphology

  • @ref: 117065
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4314COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
4314TREPONEMA MEDIUM (DSMZ Medium 159)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium159.pdf
40178MEDIUM 20 - for Anaerobic bacteriayesAgar (15.000 g);Glucose (5.000 g);Yeast extract (20.000 g);Tryptone (30.000 g);Cysteine hydrochloride (0.500 g);distilled water (1000.000 ml);Hemin solution -M00149 (25.000 ml)
117065CIP Medium 20yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=20

culture temp

@refgrowthtypetemperaturerange
4314positivegrowth37mesophilic
40178positivegrowth37mesophilic
117065positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 4314
  • oxygen tolerance: anaerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68380serine arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase+3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase+3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase-
68380alkaline phosphatase+3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase+3.2.1.55
68380beta-glucosidase+3.2.1.21
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
4314--+/-+-+++--------+--+----+--+/--
4314--+++/-+++--------+--+----+----
4314---+-+/-++--------+--+----+----
4314-----+++--------+--+----+----
4314--+++/-+++--------+--+----+----

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentisolation date
4314rumen of cowUSAUSANorth America
117065Animal, Cattle, rumenUnited States of AmericaUSANorth America1958

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Bovinae (Cow, Cattle)
#Host Body-Site#Organ#Rumen
#Host Body-Site#Gastrointestinal tract#Stomach

taxonmaps

  • @ref: 69479
  • File name: preview.99_3326.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_16745;96_1734;97_2075;98_2550;99_3326&stattab=map
  • Last taxonomy: Prevotella bryantii subclade
  • 16S sequence: NR_028866
  • Sequence Identity:
  • Total samples: 23526
  • soil counts: 406
  • aquatic counts: 489
  • animal counts: 22190
  • plant counts: 441

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
43142Risk group (German classification)
1170652Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Prevotella bryantii 16S rRNA geneAJ0064571486ena752555
4314Prevotella bryantii B14 16S ribosomal RNA, partial sequenceNR_0288661486nuccore752555

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Prevotella bryantii DSM 11371GCA_019973295scaffoldncbi77095
66792Prevotella bryantii B14GCA_900110745contigncbi752555
66792Prevotella bryantii B14GCA_000179055contigncbi752555
66792Prevotella bryantii B14752555.7wgspatric752555
66792Prevotella bryantii B14752555.5wgspatric752555
66792Prevotella bryantii strain DSM 1137177095.19wgspatric77095
66792Prevotella bryantii DSM 113712693429877draftimg752555
66792Prevotella bryantii B14648276714draftimg752555
66792Prevotella bryantii B14GCA_022024235completencbi752555
66792Prevotella bryantii strain DSM 1137177095.22wgspatric77095
66792Prevotella bryantii strain DSM 1137177095.20wgspatric77095
66792Prevotella bryantii strain DSM 1137177095.21wgspatric77095

GC content

  • @ref: 4314
  • GC-content: 39.1
  • method: sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno94.867no
gram-positiveno97.481no
anaerobicyes99.376yes
aerobicno98.535yes
halophileno89.29no
spore-formingno95.922no
motileno91.076no
glucose-fermentyes55.159no
thermophileno96.571yes
glucose-utilyes82.686no

External links

@ref: 4314

culture collection no.: DSM 11371, CIP 105474, JCM 13993

straininfo link

  • @ref: 81753
  • straininfo: 49087

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4314Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 11371)https://www.dsmz.de/collection/catalogue/details/culture/DSM-11371
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40178Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17497
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68380Automatically annotated from API rID32A
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
81753Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID49087.1StrainInfo: A central database for resolving microbial strain identifiers
117065Curators of the CIPCollection of Institut Pasteur (CIP 105474)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105474