Strain identifier

BacDive ID: 12535

Type strain: Yes

Species: Segatella albensis

Strain Designation: M384

Strain history: CIP <- 1998, G. Avgustin, Univ. Ljubljana, Slovenija: strain M384 <- R.J. Wallace, Rowett Res. Inst., Aberdeen, Scotland: strain M384, Bacteroides ruminicola subsp. ruminicola

NCBI tax ID(s): 1122978 (strain), 77768 (species)

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General

@ref: 4313

BacDive-ID: 12535

DSM-Number: 11370

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-negative

description: Segatella albensis M384 is an anaerobe, mesophilic, Gram-negative bacterium that was isolated from sheep rumen.

NCBI tax id

NCBI tax idMatching level
77768species
1122978strain

strain history

@refhistory
4313<- G. Avgustin <- R.J. Wallace, M384 (Bacteroides ruminicola subsp. ruminicola)
67770CIP 105472 <-- G. Avgustin M384 <-- R. J. Wallace M384.
116183CIP <- 1998, G. Avgustin, Univ. Ljubljana, Slovenija: strain M384 <- R.J. Wallace, Rowett Res. Inst., Aberdeen, Scotland: strain M384, Bacteroides ruminicola subsp. ruminicola

doi: 10.13145/bacdive12535.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Bacteroidia
  • order: Bacteroidales
  • family: Prevotellaceae
  • genus: Segatella
  • species: Segatella albensis
  • full scientific name: Segatella albensis (Avguštin et al. 1997) Hitch et al. 2023

  • synonyms

    • @ref: 20215
    • synonym: Prevotella albensis

@ref: 4313

domain: Bacteria

phylum: Bacteroidetes

class: Bacteroidia

order: Bacteroidales

family: Prevotellaceae

genus: Prevotella

species: Prevotella albensis

full scientific name: Prevotella albensis Avguštin et al. 1997 emend. Hahnke et al. 2016

strain designation: M384

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480negative99.999
116183negativerod-shapedno

colony morphology

@refincubation period
43131-2 days
59373>3 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4313COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
4313TREPONEMA MEDIUM (DSMZ Medium 159)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium159.pdf
39299MEDIUM 20 - for Anaerobic bacteriayesAgar (15.000 g);Glucose (5.000 g);Yeast extract (20.000 g);Tryptone (30.000 g);Cysteine hydrochloride (0.500 g);distilled water (1000.000 ml);Hemin solution -M00149 (25.000 ml)
116183CIP Medium 20yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=20

culture temp

@refgrowthtypetemperaturerange
4313positivegrowth37mesophilic
39299positivegrowth37mesophilic
59373positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
4313anaerobe
59373anaerobe
69480anaerobe99.999

spore formation

@refspore formationconfidence
69481no100
69480no100

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11618317632nitrate-reduction
11618316301nitrite-reduction
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

@refChebi-IDmetaboliteproduction
11618335581indoleno
6838035581indoleno

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68380serine arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase-
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380beta-Galactosidase 6-phosphate-
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5
116183oxidase-
116183catalase-1.11.1.6
116183urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116183-+--------++-+--+---

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
4313---+-+++--------+--+----+--+/--
4313---+-+/-++--------+--+----+----
4313---+/---+/-+/---------+/---+/-----+/-----

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
4313sheep rumenScotlandUnited KingdomGBREurope
59373Rumen of cattle
67770Rumen of cattle
116183Animal, Sheep, rumenScotlandUnited KingdomGBREurope

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Caprinae (Sheep/Goat)
#Host Body-Site#Organ#Rumen

taxonmaps

  • @ref: 69479
  • File name: preview.99_5895.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_44;96_2821;97_3467;98_4383;99_5895&stattab=map
  • Last taxonomy: Prevotella albensis subclade
  • 16S sequence: AB547669
  • Sequence Identity:
  • Total samples: 8477
  • soil counts: 66
  • aquatic counts: 61
  • animal counts: 8227
  • plant counts: 123

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
43132Risk group (German classification)
1161832Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
4313Prevotella albensis 16S rRNA gene, strain M384AJ0116831495ena77768
67770Prevotella albensis gene for 16S ribosomal RNA, partial sequence, strain: JCM 12258AB5476691481ena77768

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Prevotella albensis DSM 113701122978.3wgspatric1122978
66792Prevotella albensis JCM 122581235821.3wgspatric1122978
66792Prevotella albensis JCM 122582582581006draftimg1122978
66792Prevotella albensis DSM 113702526164514draftimg1122978
67770Prevotella albensis DSM 11370 = JCM 12258GCA_000426565scaffoldncbi1122978
67770Prevotella albensis DSM 11370 = JCM 12258GCA_000613525contigncbi1122978

GC content

  • @ref: 4313
  • GC-content: 41.2
  • method: sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno91.864no
flagellatedno96.496no
gram-positiveno97.686no
anaerobicyes99.664no
aerobicno98.784yes
halophileno85.979no
spore-formingno96.071no
thermophileno94.149yes
glucose-utilyes82.289no
glucose-fermentyes70.683no

External links

@ref: 4313

culture collection no.: CIP 105472, DSM 11370, CCUG 51935, JCM 12258

straininfo link

  • @ref: 81752
  • straininfo: 49086

Reference

@idauthorscataloguedoi/urltitle
4313Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 11370)https://www.dsmz.de/collection/catalogue/details/culture/DSM-11370
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
39299Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17495
59373Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 51935)https://www.ccug.se/strain?id=51935
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68380Automatically annotated from API rID32A
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
81752Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID49086.1StrainInfo: A central database for resolving microbial strain identifiers
116183Curators of the CIPCollection of Institut Pasteur (CIP 105472)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105472