Strain identifier

BacDive ID: 12531

Type strain: Yes

Species: Prevotella intermedia

Strain Designation: B422

Strain history: <- DSM <- ATCC <- LV Holdeman, VPI 4197 <- S Finegold, B422

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 8783

BacDive-ID: 12531

DSM-Number: 20706

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, rod-shaped, human pathogen

description: Prevotella intermedia B422 is an anaerobe, mesophilic, rod-shaped human pathogen that was isolated from empyema.

NCBI tax id

NCBI tax idMatching level
1122984strain
28131species

strain history

@refhistory
8783<- ATCC <- L.V. Holdeman, VPI 4197 <- S. Finegold, B422
67770K. Okamoto <-- ATCC 25611 <-- L. V. Holdeman VPI 4197 <-- S. Finegold B422.
67771<- DSM <- ATCC <- LV Holdeman, VPI 4197 <- S Finegold, B422

doi: 10.13145/bacdive12531.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Bacteroidia
  • order: Bacteroidales
  • family: Prevotellaceae
  • genus: Prevotella
  • species: Prevotella intermedia
  • full scientific name: Prevotella intermedia (Holdeman and Moore 1970) Shah and Collins 1990
  • synonyms

    @refsynonym
    20215Bacteroides melaninogenicus subsp. intermedius
    20215Bacteroides intermedius

@ref: 8783

domain: Bacteria

phylum: Bacteroidetes

class: Bacteroidia

order: Bacteroidales

family: Prevotellaceae

genus: Prevotella

species: Prevotella intermedia

full scientific name: Prevotella intermedia (Holdeman and Moore 1970) Shah and Collins 1990 emend. Hahnke et al. 2016

strain designation: B422

type strain: yes

Morphology

cell morphology

@refcell shapegram stainconfidence
67771rod-shaped
67771negative
69480negative99.998

colony morphology

  • @ref: 8783
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8783COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
8783PYG MEDIUM (MODIFIED) (DSMZ Medium 104)yeshttps://mediadive.dsmz.de/medium/104Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water

culture temp

@refgrowthtypetemperaturerange
8783positivegrowth37mesophilic
67770positivegrowth37mesophilic
67771positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
8783anaerobe
67771anaerobe
69480anaerobe99.599

spore formation

@refspore formationconfidence
69481no100
69480no99.998

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836716634raffinose+builds acid from
6836716024D-mannose+builds acid from
6836717057cellobiose-builds acid from
683675291gelatin+hydrolysis
6836717306maltose+builds acid from
6836717992sucrose+builds acid from
6836717716lactose-builds acid from
6836717634D-glucose+builds acid from
6836716199urea-hydrolysis
6836727897tryptophan+energy source
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838029985L-glutamate-degradation
6838017632nitrate-reduction

metabolite production

  • @ref: 68367
  • Chebi-ID: 35581
  • metabolite: indole
  • production: yes

metabolite tests

  • @ref: 68367
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: +

enzymes

@refvalueactivityec
68380glycin arylamidase-
68380alanine arylamidase+3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase+3.4.11.1
68380alkaline phosphatase+3.1.3.1
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase-3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5
68367catalase-1.11.1.6
68367gelatinase+
68367urease-3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    47910C14:01.814
    47910C16:07.816
    47910C18:03.218
    47910C13:0 iso1.712.612
    47910C14:0 ISO12.413.618
    47910C15:0 ANTEISO17.914.711
    47910C15:0 ISO10.714.621
    47910C16:0 iso2.515.626
    47910C17:0 iso0.916.629
    47910C17:0 iso 3OH8.518.161
    47910C18:2 ω6,9c/C18:0 ANTE32.617.724
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API 20A

@refINDUREGLUMANLACSACMALSALXYLARAGELESCGLYCELMNEMLZRAFSORRHATRECAT
8783+-++-++++++++-++++++-
8783+-+--++---+---+-+---

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
8783-----+-------+-++++/-+----+-+/-+-
8783-----+----+/-+/--+-+++-+----+--+-
47910-----+-------+--++-+----+-++-
8783-----+-----+/--+-+++/--+----+--+/--
8783-----+-----+/----++--+----+--+-
8783-----+----------++-+----+----
8783-----+-----+/----+++-+----+--+-
8783-----+/-----------++-+----+--+/-+

Isolation, sampling and environmental information

isolation

@refsample type
8783empyema
47910Empyema
67770Empyema
67771From empyema

isolation source categories

  • Cat1: #Host Body Product
  • Cat2: #Fluids

taxonmaps

  • @ref: 69479
  • File name: preview.99_2089.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_295;96_1148;97_1348;98_1632;99_2089&stattab=map
  • Last taxonomy: Prevotella intermedia subclade
  • 16S sequence: X73965
  • Sequence Identity:
  • Total samples: 66903
  • soil counts: 1452
  • aquatic counts: 1863
  • animal counts: 62951
  • plant counts: 637

Safety information

risk assessment

  • @ref: 8783
  • pathogenicity human: yes
  • pathogenicity animal: yes
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Prevotella intermedia 16S-23S ribosomal RNA intergenic spacer, partial sequenceAF385927431ena1122984
20218Prevotella intermedia strain ATCC 25611 16S ribosomal RNA gene, partial sequenceAF4148211364ena1122984
20218Prevotella intermedia ATCC 25611 16S ribosomal RNA gene, complete sequenceL164681473ena28131
20218P.intermedia gene for 16S rRNAX739651459ena1122984
8783Prevotella intermedia strain ATCC 25611 16S ribosomal RNA gene, partial sequenceFJ7925351370ena1122984
67770Prevotella intermedia gene for 16S ribosomal RNA, partial sequence, strain: JCM 11150AB5476871380ena1122984

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Prevotella intermedia ATCC 25611 = DSM 20706GCA_001953955completencbi1122984
66792Prevotella intermedia ATCC 256111122984.4wgspatric1122984
66792Prevotella intermedia ATCC 25611 = DSM 20706 strain ATCC 256111122984.13completepatric1122984
66792Prevotella intermedia ATCC 25611 [PRJNA169798]1122984.7wgspatric1122984
66792Prevotella intermedia strain NCTC1307028131.49wgspatric28131
66792Prevotella intermedia strain strain 1728131.7completepatric28131
66792Prevotella intermedia ATCC 256112515154173draftimg1122984
66792Prevotella intermedia ATCC 256112816332597completeimg1122984
66792Prevotella intermedia NCTC 130702903615608draftimg28131
66792Prevotella intermedia DSM 207062523231016draftimg1122984
67770Prevotella intermedia ATCC 25611 = DSM 20706GCA_000519165scaffoldncbi1122984
67771Prevotella intermedia ATCC 25611 = DSM 20706GCA_000439065scaffoldncbi1122984
67771Prevotella intermedia NCTC13070GCA_900455005contigncbi28131

GC content

@refGC-contentmethod
878343.3sequence analysis
6777044.2high performance liquid chromatography (HPLC)
6777143.3genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno97.282no
gram-positiveno97.021yes
anaerobicyes98.913no
halophileno85.072no
spore-formingno96.868no
glucose-utilyes81.858no
aerobicno98.358yes
thermophileno97.534yes
motileno92.134no
glucose-fermentyes53.022no

External links

@ref: 8783

culture collection no.: DSM 20706, ATCC 25611, KCTC 15693, CCUG 24041, JCM 7365, JCM 12248, VPI 4197, JCM 11150, BCRC 14416, CIP 103682, NCTC 13070, CIP 101222, KCTC 3692

straininfo link

  • @ref: 81750
  • straininfo: 38613

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny1390107Biochemical and chemical studies on strains designated Prevotella intermedia and proposal of a new pigmented species, Prevotella nigrescens sp. nov.Shah HN, Gharbia SEInt J Syst Bacteriol10.1099/00207713-42-4-5421992Bacterial Typing Techniques, Bacteroides/*classification/genetics/metabolism, Cellobiose/metabolism, Culture Media, DNA, Bacterial/chemistry, Fermentation, Nucleic Acid Hybridization, Phenotype, Pigmentation, Xylans/metabolismMetabolism
Phylogeny1528627Phenotypic characteristics and DNA relatedness in Prevotella intermedia and similar organisms.Fukushima H, Moroi H, Inoue J, Onoe T, Ezaki T, Yabuuchi E, Leung KP, Walker CB, Clark WB, Sagawa HOral Microbiol Immunol10.1111/j.1399-302x.1992.tb00023.x1992Bacteroides/*classification/*genetics, DNA Probes, DNA, Bacterial/analysis, Humans, Nucleic Acid Hybridization, Periodontal Diseases/microbiology, Phenotype, Saliva/microbiologyPathogenicity
Phylogeny1920011Isolation and purification of bacteriocin from Prevotella intermedia (Bacteroides intermedius).Takada K, Hirasawa M, Ikeda TJ Periodontol10.1902/jop.1991.62.7.4391991Adult, Antibiosis, Bacteriocins/*isolation & purification/pharmacology, Bacteroides/classification/*metabolism, Colony Count, Microbial, Culture Media, Fusobacterium nucleatum/drug effects, Gingival Crevicular Fluid/microbiology, Humans, Molecular Weight, Periodontitis/microbiologyPathogenicity
Pathogenicity2134944[Mitogenic activity of lipopolysaccharide from Bacteroides intermedius against C3H/HeJ mice spleen cells].Yamamoto M, Ono K, Mochizuki S, Nishihara Y, Makimura M, Otake SNichidai Koko Kagaku1990Animals, *Bacteroides, Cell Division/drug effects, Cells, Cultured, *Lipopolysaccharides, Mice, Mice, Inbred BALB C, Mice, Inbred C3H, Mitogens/*pharmacology, Polymyxin B/pharmacology, Spleen/cytology/*drug effects, Thymus Gland/cytology/drug effects
Metabolism2562264[Effects of endotoxins from periodontopathic bacteria on the collagen metabolism of cultured normal human gingival fibroblasts].Tagata YNihon Shishubyo Gakkai Kaishi10.2329/perio.31.7551989Bacteroides, Collagen/*metabolism, Endotoxins/*pharmacokinetics, Fibroblasts/metabolism, Gingiva/cytology, Humans, Microbial Collagenase/metabolism, Periodontal Diseases/microbiologyPathogenicity
Pathogenicity2626166[Toxicity of Bacteroides intermedius surface extracts against mouse splenic lymphocyte].Kuroki T, Kato H, Nishihara Y, Nakagawa S, Makimura M, Otake SNichidai Koko Kagaku1989Animals, Bacterial Proteins/*toxicity, *Bacteroides, Cell Line, Lymphocytes/*drug effects, Membrane Proteins/*toxicity, Mice
Pathogenicity2640314Surface appendages, hemagglutination, and adherence to human epithelial cells of Bacteroides intermedius.Leung KP, Fukushima H, Sagawa H, Walker CB, Clark WBOral Microbiol Immunol10.1111/j.1399-302x.1989.tb00253.x1989Animals, *Bacterial Adhesion, Bacteroides/physiology/*ultrastructure, Cell Membrane/*ultrastructure, Epithelial Cells, *Hemagglutination, Humans, Mouth Mucosa/cytology
Phylogeny2902111The use of whole-cell DNA probes for the identification of Bacteroides intermedius isolates in a dot blot assay.Moncla BJ, Strockbine L, Braham P, Karlinsey J, Roberts MCJ Dent Res10.1177/002203458806701004011988Bacteroides/*classification/genetics/isolation & purification, *DNA Probes, Nucleic Acid Hybridization, Prevotella melaninogenica/*classification/genetics/isolation & purification, Sequence Homology, Nucleic Acid, Species SpecificityEnzymology
Pathogenicity7560233Effect of periodontal treatments on serum IgG antibody titers against periodontopathic bacteria.Horibe M, Watanabe H, Ishikawa IJ Clin Periodontol10.1111/j.1600-051x.1995.tb00798.x1995Adult, Aggregatibacter actinomycetemcomitans/immunology, Alveolar Bone Loss/microbiology/surgery/therapy, Antibodies, Bacterial/*blood, Bacteria/*immunology, Capnocytophaga/immunology, Dental Scaling, Eikenella corrodens/immunology, Female, Fusobacterium nucleatum/immunology, Humans, Immunoglobulin G/*blood, Male, Middle Aged, Periodontal Pocket/microbiology/surgery/therapy, Periodontitis/*microbiology/surgery/*therapy, Porphyromonas gingivalis/immunology, Prevotella/immunology, Prevotella intermedia/immunology, Root Planing
Enzymology7796488Properties of alkaline phosphatase in the gingival crevicular fluid.Kina JR, Yoshida N, Goseki M, Sasaki S, Ishikawa IBull Tokyo Med Dent Univ1995Aged, Alkaline Phosphatase/*chemistry/metabolism, Electrophoresis, Enzyme Activation, Female, Gingival Crevicular Fluid/*enzymology, Humans, Isoenzymes/*chemistry/metabolism, Male, Middle Aged, Periodontitis/enzymologyMetabolism
Pathogenicity8188371Immunobiological activities of a 55-kilodalton cell surface protein of Prevotella intermedia ATCC 25611.Matsushita K, Nagaoka S, Arakaki R, Kawabata Y, Iki K, Kawagoe M, Takada HInfect Immun10.1128/iai.62.6.2459-2469.19941994Amino Acid Sequence, Animals, Antigens, Surface/immunology, Bacterial Proteins/chemistry/*immunology/pharmacology, Bacteroides/*immunology, Cell Line, Cytokines/biosynthesis, Female, Humans, Mice, Mice, Inbred BALB C, Mice, Inbred C3H, Molecular Sequence Data, Molecular WeightGenetics
Metabolism9345789Fermentation product analysis in the identification of black-pigmented bacteria from periodontitis.Takada K, Hirasawa MMicrobios1997Antibodies, Bacterial/analysis/immunology, Antigens, Bacterial/analysis/immunology, Bacteriological Techniques, Chromatography, Gas, Culture Media/*analysis/metabolism, Electrophoresis, Polyacrylamide Gel, Fatty Acids/*analysis/metabolism, Fermentation, Gram-Negative Anaerobic Bacteria/growth & development/immunology/*metabolism, Humans, Immunodiffusion, Periodontitis/*microbiologyEnzymology
Pathogenicity9353030A novel component different from endotoxin extracted from Prevotella intermedia ATCC 25611 activates lymphoid cells from C3H/HeJ mice and gingival fibroblasts from humans.Iki K, Kawahara K, Sawamura S, Arakaki R, Sakuta T, Sugiyama A, Tamura H, Sueda T, Hamada S, Takada HInfect Immun10.1128/iai.65.11.4531-4538.19971997Adjuvants, Immunologic/*analysis, Animals, Cytokines/biosynthesis, Fibroblasts/drug effects/metabolism, Gingiva/cytology, Glycoproteins/*analysis/pharmacology, Humans, Lipopolysaccharides/pharmacology, *Lymphocyte Activation, Mice, Mice, Inbred C3H, Polymyxin B/pharmacology, Prevotella intermedia/*chemistryMetabolism
Pathogenicity9354029Anti-bacterial action of onion (Allium cepa L.) extracts against oral pathogenic bacteria.Kim JHJ Nihon Univ Sch Dent10.2334/josnusd1959.39.13619971-Butanol/chemistry, Acetic Acid, Adult, Alkalies/pharmacology, Anti-Bacterial Agents/analysis/antagonists & inhibitors/*pharmacology, Cellulose, Chromatography, Thin Layer, Culture Media, Cysteine/pharmacology, Dental Caries/microbiology, Humans, Indicators and Reagents, Ninhydrin, *Onions/chemistry, Periodontitis/microbiology, Plant Extracts/analysis/antagonists & inhibitors/*pharmacology, Porphyromonas gingivalis/*drug effects, Prevotella intermedia/*drug effects, Solvents/chemistry, Steam, Streptococcus mutans/*drug effects, Streptococcus sobrinus/*drug effects, Time FactorsCultivation
Pathogenicity9467379Acid tolerance response and survival by oral bacteria.Svensater G, Larsson UB, Greif EC, Cvitkovitch DG, Hamilton IROral Microbiol Immunol10.1111/j.1399-302x.1997.tb00390.x1997Acids/*metabolism, Actinomyces/growth & development/*physiology, Adaptation, Biological, Culture Media/chemistry, Drug Tolerance, Humans, Hydrogen-Ion Concentration, Lactobacillus/growth & development/*physiology, Mouth/*microbiology, Neisseria/growth & development, Prevotella intermedia/growth & development, Streptococcus/growth & development/*physiologyMetabolism
Phylogeny9542074Biochemical and genetic characterization of a Prevotella intermedia/nigrescens-like organism.Kononen E, Matto J, Vaisanen-Tunkelrott ML, Frandsen EV, Helander I, Asikainen S, Finegold SM, Jousimies-Somer HInt J Syst Bacteriol10.1099/00207713-48-1-391998Bacterial Proteins/analysis, Classification, DNA, Bacterial/analysis, Electrophoresis, Genetic Heterogeneity, Glutamate Dehydrogenase/analysis, Immunoglobulin A/metabolism, Malate Dehydrogenase/analysis, Oligonucleotide Probes, Polymerase Chain Reaction, Prevotella intermedia/classification/*enzymology/*genetics, Species Specificity, beta-Lactamases/metabolismEnzymology
Enzymology9654126Purification and characterization of alkaline phosphatase containing phosphotyrosyl phosphatase activity from the bacterium Prevotella intermedia.Ansai T, Awano S, Chen X, Fuchi T, Arimoto T, Akifusa S, Takehara TFEBS Lett10.1016/s0014-5793(98)00514-61998Alkaline Phosphatase/chemistry/isolation & purification/*metabolism, Amino Acid Sequence, Chromatography, Gel, Chromatography, Ion Exchange, Electrophoresis, Polyacrylamide Gel, Enzyme Stability, Hot Temperature, Molecular Sequence Data, Peptide Fragments/chemistry, Prevotella intermedia/*enzymology, Protein Tyrosine Phosphatases/isolation & purification/*metabolism, Sequence Alignment, Sequence Homology, Amino Acid, Substrate Specificity, ThermodynamicsPhylogeny
Pathogenicity9719033Dual regulatory effects of interferon-alpha, -beta, and -gamma on interleukin-8 gene expression by human gingival fibroblasts in culture upon stimulation with lipopolysaccharide from Prevotella intermedia, interleukin-1alpha, or tumor necrosis factor-alpha.Sakuta T, Tokuda M, Tamura M, Jimi E, Ikebe T, Koba T, Nagaoka S, Takada HJ Dent Res10.1177/002203459807700807011998Blotting, Northern, Cells, Cultured, Child, Enzyme-Linked Immunosorbent Assay, Fibroblasts/drug effects/metabolism, Gene Expression Regulation, Gingiva/cytology/*drug effects/metabolism, Humans, Interferon-alpha/pharmacology, Interferon-beta/pharmacology, Interferon-gamma/pharmacology, Interferons/*pharmacology, Interleukin-1/pharmacology, Interleukin-8/*biosynthesis/genetics, Lipopolysaccharides/antagonists & inhibitors/*pharmacology, Male, NF-kappa B/physiology, Prevotella intermedia/chemistry, Protein Synthesis Inhibitors/pharmacology, RNA, Messenger/analysis, Recombinant Proteins/pharmacology, Tosylphenylalanyl Chloromethyl Ketone/pharmacology, Tumor Necrosis Factor-alpha/pharmacology, Up-RegulationMetabolism
Phylogeny10074526Use of PCR and sodium dodecyl sulfate-polyacrylamide gel electrophoresis techniques for differentiation of Prevotella intermedia sensu stricto and Prevotella nigrescens.Premaraj T, Kato N, Fukui K, Kato H, Watanabe KJ Clin Microbiol10.1128/JCM.37.4.1057-1061.19991999*Bacterial Typing Techniques, Base Sequence, DNA Primers/genetics, Electrophoresis, Polyacrylamide Gel/*methods, Evaluation Studies as Topic, Humans, Polymerase Chain Reaction/*methods, Prevotella/*classification/*genetics/isolation & purification, Prevotella intermedia/*classification/*genetics/isolation & purification, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Reproducibility of Results, Sodium Dodecyl Sulfate, Species SpecificityEnzymology
Phylogeny10559178Isolation, cloning, and expression of an acid phosphatase containing phosphotyrosyl phosphatase activity from Prevotella intermedia.Chen X, Ansai T, Awano S, Iida T, Barik S, Takehara TJ Bacteriol10.1128/JB.181.22.7107-7114.19991999Acid Phosphatase/chemistry/*genetics/*isolation & purification/metabolism, Amino Acid Sequence, Base Sequence, Cloning, Molecular, Molecular Sequence Data, Phosphorylation, Phylogeny, Prevotella intermedia/*enzymology/genetics, Protein Tyrosine Phosphatases/chemistry/*genetics/*metabolism, Recombinant Proteins/metabolism, Sequence Analysis, DNAEnzymology
Phylogeny10635221Survey for collagenase gene prtC in Porphyromonas gingivalis and Porphyromonas endodontalis isolated from endodontic infections.Odell LJ, Baumgartner JC, Xia T, David LLJ Endod10.1016/S0099-2399(99)80379-31999*Bacterial Proteins, Bacterial Typing Techniques, Bacteroidaceae Infections/microbiology, Collagenases/*genetics, DNA, Bacterial/genetics, Dental Pulp Cavity/microbiology, Dental Pulp Diseases/*microbiology, *Genes, Bacterial, Humans, Microbial Collagenase/*genetics, Polymerase Chain Reaction, Polymorphism, Restriction Fragment Length, Porphyromonas/classification/*enzymology/*genetics, Porphyromonas gingivalis/classification/enzymology/genetics, RNA, Ribosomal, 16S/geneticsPathogenicity
Pathogenicity10726523Inflammatory cytokine production and specific antibody responses to lipopolysaccharide from endodontopathic black-pigmented bacteria in patients with multilesional periapical periodontitis.Matsushita K, Tajima T, Tomita K, Takada H, Nagaoka S, Torii MJ Endod10.1016/S0099-2399(99)80299-41999Adult, Antibodies, Bacterial/biosynthesis/blood, Case-Control Studies, Culture Media, Escherichia coli/chemistry/immunology, Fusobacterium nucleatum/chemistry/immunology, Humans, Interleukin-1/biosynthesis, Interleukin-6/biosynthesis, Interleukin-8/biosynthesis, Interleukins/*biosynthesis, Lipopolysaccharides/*immunology, Periapical Periodontitis/blood/*immunology/metabolism/*microbiology, Porphyromonas/chemistry/immunology, Prevotella intermedia/chemistry/immunologyCultivation
Metabolism11447173Monocytic cell activation by Nonendotoxic glycoprotein from Prevotella intermedia ATCC 25611 is mediated by toll-like receptor 2.Sugawara S, Yang S, Iki K, Hatakeyama J, Tamai R, Takeuchi O, Akashi S, Espevik T, Akira S, Takada HInfect Immun10.1128/IAI.69.8.4951-4957.20012001Acidic Glycosphingolipids/metabolism, *Acute-Phase Proteins, Animals, Antibodies, Monoclonal/metabolism/pharmacology, Bacterial Proteins/*metabolism, Calcitriol/analogs & derivatives/pharmacology, Carrier Proteins/metabolism, *Drosophila Proteins, Glycoproteins/*metabolism, Interleukin-8/biosynthesis, Lipopolysaccharide Receptors/biosynthesis, Lipopolysaccharides/pharmacology, Macrophages, Peritoneal/metabolism, Membrane Glycoproteins/biosynthesis/*metabolism, Mice, Mice, Inbred C3H, Mice, Knockout, Monocytes/drug effects/immunology/metabolism, Prevotella intermedia/*metabolism, Receptors, Cell Surface/biosynthesis/*metabolism, Toll-Like Receptor 2, Toll-Like Receptor 4, Toll-Like Receptors, Tumor Cells, Cultured, Tumor Necrosis Factor-alpha/biosynthesisPathogenicity
Pathogenicity12479628In vitro effect of laser irradiation on cementum-bound endotoxin isolated from periodontally diseased roots.Liu CM, Shyu YC, Pei SC, Lan WH, Hou LTJ Periodontol10.1902/jop.2002.73.11.12602002Adult, Aggregatibacter actinomycetemcomitans/chemistry, Bacteroides/chemistry, Chi-Square Distribution, Dental Cementum/*microbiology, Endotoxins/*radiation effects, Humans, In Vitro Techniques, Interleukin-1/biosynthesis, *Laser Therapy, Limulus Test, Monocytes/metabolism, Neodymium, Periodontitis/*microbiology/*therapy, Porphyromonas gingivalis/chemistry, Statistics, Nonparametric, Treatment FailureMetabolism
Metabolism12728301Cleavage of CD14 and LBP by a protease from Prevotella intermedia.Deschner J, Singhal A, Long P, Liu CC, Piesco N, Agarwal SArch Microbiol10.1007/s00203-003-0548-12003*Acute-Phase Proteins, Animals, Carrier Proteins/*metabolism, Cells, Cultured, Cysteine Endopeptidases/analysis/*metabolism, Cysteine Proteinase Inhibitors/metabolism, Electrophoresis, Polyacrylamide Gel, Humans, Lipopolysaccharide Receptors/*metabolism, Lipopolysaccharides/metabolism, *Membrane Glycoproteins, Mice, Monocytes/metabolism, Prevotella intermedia/*enzymology/metabolism, Time Factors, U937 CellsEnzymology
Enzymology12753473Purification and partial characterization of a dipeptidyl peptidase from Prevotella intermedia.Shibata Y, Miwa Y, Hirai K, Fujimura SOral Microbiol Immunol10.1034/j.1399-302x.2003.00057.x2003Amino Acid Sequence, Bacterial Proteins/isolation & purification/metabolism, Dipeptidyl Peptidase 4/*isolation & purification/metabolism, Electrophoresis, Polyacrylamide Gel, Hydrogen-Ion Concentration, Molecular Weight, Prevotella intermedia/*enzymology/isolation & purification, Virulence FactorsPhylogeny
Enzymology12753913Chemical structure and immunobiological activity of lipid A from Prevotella intermedia ATCC 25611 lipopolysaccharide.Hashimoto M, Asai Y, Tamai R, Jinno T, Umatani K, Ogawa TFEBS Lett10.1016/s0014-5793(03)00414-92003Animals, Cells, Cultured, Interleukin-6/biosynthesis, Lipid A/*chemistry/isolation & purification/*pharmacology, Mass Spectrometry, Mice, Mice, Inbred C3H, NF-kappa B/metabolism, Nuclear Magnetic Resonance, Biomolecular, Prevotella intermedia/*chemistryPhylogeny
Pathogenicity12937592Coronal microleakage assessed by polymicrobial markers.Britto LR, Grimaudo NJ, Vertucci FJJ Contemp Dent Pract1526-3711-1122003Analysis of Variance, Biomarkers, Dental Leakage/*microbiology/prevention & control, Dental Pulp Cavity/*microbiology, Humans, Prevotella intermedia/isolation & purification/physiology, *Root Canal Filling Materials, Root Canal Obturation/*methods, Saliva/microbiology, Saliva, Artificial, Statistics, Nonparametric, Streptococcus/isolation & purification/physiology, Tooth Crown/microbiology, Tooth, Nonvital/*microbiologyEnzymology
Metabolism14759706In vitro activation of the hemolysin in Prevotella nigrescens ATCC 33563 and Prevotella intermedia ATCC 25611.Silva TA, Noronha FS, de Macedo Farias L, Carvalho MARes Microbiol10.1016/j.resmic.2003.09.0102004Animals, Edetic Acid/pharmacology, Endopeptidase K/metabolism, Endopeptidases/metabolism, Enzyme Activation, Enzyme Inhibitors/pharmacology, Enzyme Stability, Hemolysin Proteins/*metabolism, *Hemolysis, Humans, Hydrogen-Ion Concentration, Kinetics, Leucine/*analogs & derivatives/pharmacology, Pepstatins/pharmacology, Phenylmethylsulfonyl Fluoride/pharmacology, Prevotella intermedia/growth & development/*metabolism, Prevotella nigrescens/growth & development/*metabolism, Rabbits, Temperature, Tosyllysine Chloromethyl Ketone/pharmacology, Trypsin/metabolismEnzymology
Pathogenicity15491347Prevotella intermedia lipopolysaccharide stimulates release of nitric oxide by inducing expression of inducible nitric oxide synthase.Kim SJ, Ha MS, Choi EY, Choi JI, Choi ISJ Periodontal Res10.1111/j.1600-0765.2004.00757.x2004Animals, Blotting, Western, Cell Line, Enzyme Induction/drug effects, Gene Expression, Genistein/toxicity, Indoles/toxicity, Lipopolysaccharides/*pharmacology, Macrophages/drug effects/*enzymology/microbiology, Maleimides/toxicity, Mice, Microtubules/physiology, Nitric Oxide/*biosynthesis/toxicity, Nitric Oxide Synthase/*biosynthesis, Nitric Oxide Synthase Type II, Prevotella intermedia/*pathogenicity, Protein Kinase Inhibitors/toxicity, RNA, Messenger/analysis, Reverse Transcriptase Polymerase Chain ReactionEnzymology
Pathogenicity16490986The efficacy of povidone-iodine products against periodontopathic bacteria.Nakagawa T, Hosaka Y, Ishihara K, Hiraishi T, Sato S, Ogawa T, Kamoi KDermatology10.1159/0000892082006Aggregatibacter actinomycetemcomitans/drug effects, Anti-Infective Agents, Local/*pharmacology, Fusobacterium nucleatum/drug effects, Gram-Negative Bacteria/*drug effects, Humans, Mouth/*microbiology, Porphyromonas gingivalis/drug effects, Povidone-Iodine/*pharmacology, Prevotella intermedia/drug effects, Streptococcus anginosus/*drug effects
Pathogenicity16673792Antimicrobial effect in vitro of chlorhexidine and calcium hydroxide impregnated gutta-percha points.Bozza FL, Molgatini SL, Perez SB, Tejerina DP, Perez Tito RI, Kaplan AEActa Odontol Latinoam2005Anti-Infective Agents, Local/*pharmacology, Calcium Hydroxide/*pharmacology, Candida albicans/drug effects, Chlorhexidine/*pharmacology, Colony Count, Microbial, Culture Media, Enterococcus faecalis/drug effects, Fusobacterium nucleatum/drug effects, *Gutta-Percha, Humans, Hydrogen-Ion Concentration, Materials Testing, Oxygen, Porphyromonas gingivalis/drug effects, Prevotella intermedia/drug effects, *Root Canal Filling Materials, Staphylococcus aureus/drug effects, Streptococcus mutans/drug effects, Temperature, Time FactorsCultivation
Metabolism16806047Antimicrobial actions of benzimidazoles against the oral anaerobes Fusobacterium nucleatum and Prevotella intermedia.Sheng J, Nguyen PT, Baldeck JD, Olsson J, Marquis REArch Oral Biol10.1016/j.archoralbio.2006.05.00220062-Pyridinylmethylsulfinylbenzimidazoles/*pharmacology, Amino Acids/metabolism, Aminopeptidases/antagonists & inhibitors, Anaerobiosis/physiology, Anti-Infective Agents/*pharmacology, Enzyme Inhibitors/pharmacology, Fermentation, Fructose/metabolism, Fusobacterium nucleatum/*drug effects/metabolism, Glucose, Glutamic Acid/metabolism, Humans, Hydrogen-Ion Concentration, Lansoprazole, Lysine/metabolism, Mouth/*microbiology, Oxygen/metabolism, Prevotella intermedia/*drug effects/metabolism, Serine/metabolismPathogenicity
16887668Susceptibility of Prevotella intermedia/Prevotella nigrescens (and Porphyromonas gingivalis) to propolis (bee glue) and other antimicrobial agents.Santos FA, Bastos EM, Rodrigues PH, de Uzeda M, de Carvalho MA, Farias Lde M, Moreira ESAnaerobe10.1006/anae.2002.04112002
Pathogenicity17064394The effects of tetracycline, minocycline, doxycycline and ofloxacin on Prevotella intermedia biofilm.Takahashi N, Ishihara K, Kimizuka R, Okuda K, Kato TOral Microbiol Immunol10.1111/j.1399-302X.2006.00305.x2006Adenosine Triphosphate/metabolism, Anti-Bacterial Agents/*pharmacology, Biofilms/*drug effects, Colony Count, Microbial, Doxycycline/pharmacology, Luminescence, Microbial Sensitivity Tests, Minocycline/pharmacology, Ofloxacin/*pharmacology, Prevotella intermedia/*drug effects, Tetracycline/pharmacology, Tetracyclines/*pharmacologyMetabolism
Metabolism17120973Effects of sonicated Prevotella intermedia, Fusobacterium nucleatum and Lactobacillus casei extracts on interleukin-8 production by human dental pulp cells.Thaweboon B, Thaweboon S, Choonharuangdej S, Suppakpatana PSoutheast Asian J Trop Med Public Health2006Adolescent, Adult, Bacterial Infections/*metabolism, Cell Survival, Cells, Cultured, Dental Pulp/metabolism/*microbiology, *Fusobacteria, Humans, Interleukin-8/*biosynthesis, *Lactobacillus casei, *Prevotella intermediaPathogenicity
Metabolism17229113Hemin nutritional stress inhibits bacterial invasion of radicular dentine by two endodontic anaerobes.Love RMInt Endod J10.1111/j.1365-2591.2006.01186.x2007Bacteria, Anaerobic/*metabolism, Culture Media, Dental Pulp Cavity/*microbiology, Dentin/*microbiology, Hemin/*metabolism, Humans, Peptostreptococcus/metabolism, Prevotella intermedia/metabolismCultivation
Cultivation17584471Production of antagonistic substance by Eikenella corrodens isolated from the oral cavity of human beings with and without periodontal disease.Apolonio AC, Carvalho MA, Ribas RN, Sousa-Gaia LG, Santos KV, Lana MA, Nicoli JR, Farias LMJ Appl Microbiol10.1111/j.1365-2672.2006.03211.x2007Acute Disease, *Antibiosis, Bacteriocins/*biosynthesis, Bacteriological Techniques, Chronic Disease, Culture Media, Eikenella corrodens/*metabolism/physiology, Gingiva/microbiology, Humans, Microbial Sensitivity Tests/methods, Mouth/*microbiology, Periodontitis/*microbiologyPathogenicity
Pathogenicity17727652Prevotella intermedia lipopolysaccharide stimulates release of tumor necrosis factor-alpha through mitogen-activated protein kinase signaling pathways in monocyte-derived macrophages.Kim SJ, Choi EY, Kim EG, Shin SH, Lee JY, Choi JI, Choi ISFEMS Immunol Med Microbiol10.1111/j.1574-695X.2007.00318.x2007Blotting, Western, Cell Line, Gene Expression, Humans, Lipopolysaccharides/*immunology, *MAP Kinase Signaling System, Macrophages/*immunology/*microbiology, Prevotella intermedia/*immunology/metabolism, RNA, Messenger/biosynthesis, Reverse Transcriptase Polymerase Chain Reaction, Tumor Necrosis Factor-alpha/genetics/*metabolismMetabolism
Pathogenicity17897190The influence of molecular oxygen exposure on the biology of Prevotella intermedia, with emphasis on its antibiotic susceptibility.dos Santos SG, Diniz CG, da Silva VL, Souza NC, de Lima FL, Bomfim MR, de Carvalho MA, Farias LMJ Appl Microbiol10.1111/j.1365-2672.2007.03313.x2007Adaptation, Physiological, Anti-Bacterial Agents/*pharmacology, Bacterial Proteins/metabolism, Drug Resistance, Bacterial, Electrophoresis, Gel, Two-Dimensional/methods, Gene Expression Regulation, Bacterial, Genetic Variation, Humans, Microbial Sensitivity Tests, Oxidative Stress, Oxygen/*pharmacology, Polymerase Chain Reaction/methods, Prevotella intermedia/*drug effects/genetics/physiologyEnzymology
Enzymology18400000Prevotella intermedia induces matrix metalloproteinase-9 expression in human periodontal ligament cells.Guan SM, Shu L, Fu SM, Liu B, Xu XL, Wu JZFEMS Microbiol Lett10.1111/j.1574-6968.2008.01140.x2008Adolescent, Anthracenes/pharmacology, Bacteroidaceae Infections/*microbiology, Butadienes/pharmacology, Cells, Cultured, Child, Culture Media, Conditioned, Electrophoresis, Polyacrylamide Gel, Extracellular Signal-Regulated MAP Kinases/antagonists & inhibitors, Humans, Imidazoles/pharmacology, Interleukin-1beta/pharmacology, JNK Mitogen-Activated Protein Kinases/antagonists & inhibitors, Lipopolysaccharides/pharmacology, Matrix Metalloproteinase 9/*biosynthesis/metabolism, Nitriles/pharmacology, Periodontal Ligament/*enzymology/*microbiology, Porphyromonas gingivalis/pathogenicity, Prevotella intermedia/*pathogenicity, Protein Kinase Inhibitors/pharmacology, Pyrimidines/pharmacology, Reverse Transcriptase Polymerase Chain Reaction, Time Factors, p38 Mitogen-Activated Protein Kinases/antagonists & inhibitorsCultivation
Pathogenicity18705525[Study of adherence of periodontal pathogens to collagen-treated hydroxyapatite].Sun CJ, Yang H, Lei CF, Xu Y, Wu YFHua Xi Kou Qiang Yi Xue Za Zhi2008*Aggregatibacter actinomycetemcomitans, Bacteria, Collagen, Durapatite, Fusobacterium nucleatum, Humans, Periodontitis, *Porphyromonas gingivalis, Prevotella intermedia
Pathogenicity18814211Antibacterial activity of [10]-gingerol and [12]-gingerol isolated from ginger rhizome against periodontal bacteria.Park M, Bae J, Lee DSPhytother Res10.1002/ptr.24732008Anti-Bacterial Agents/*pharmacology, Catechols/chemistry/*pharmacology, Chromatography, High Pressure Liquid, Fatty Alcohols/chemistry/*pharmacology, Ginger/chemistry, Humans, Microbial Sensitivity Tests, Periodontitis/microbiology, Plant Extracts/pharmacology, Porphyromonas endodontalis/*drug effects, Porphyromonas gingivalis/*drug effects, Prevotella intermedia/*drug effects, Spectrometry, Mass, Electrospray Ionization
Phylogeny19196771Prevotella falsenii sp. nov., a Prevotella intermedia-like organism isolated from monkey dental plaque.Sakamoto M, Kumada H, Hamada N, Takahashi Y, Okamoto M, Bakir MA, Benno YInt J Syst Evol Microbiol10.1099/ijs.0.002626-02009Animals, Dental Plaque/*microbiology, Fatty Acids/analysis, Macaca fascicularis/*microbiology, Molecular Sequence Data, Phylogeny, Prevotella/chemistry/*classification/genetics, RNA, Ribosomal, 16S/genetics, Species SpecificityGenetics
Pathogenicity19405834Decreased interleukin-2 responses to Fusobacterium nucleatum and Porphyromonas gingivalis in generalized aggressive periodontitis.Borch TS, Lobner M, Bendtzen K, Holmstrup P, Nielsen CHJ Periodontol10.1902/jop.2009.0805132009Adolescent, Adult, Aggressive Periodontitis/*immunology/*microbiology, Case-Control Studies, Cells, Cultured, Cytokines/biosynthesis, Female, Fusobacterium nucleatum/immunology, Humans, Interferon-gamma/biosynthesis, Interleukin-2/biosynthesis/*immunology, Lymphocyte Activation/immunology, Male, Porphyromonas gingivalis/immunology, Prevotella intermedia/immunology, Smoking/adverse effects/metabolism, T-Lymphocytes, Helper-Inducer/*immunology/metabolism, Tetanus Toxoid/immunology, Young AdultMetabolism
19457170IL-17 is involved in bone resorption in mouse periapical lesions.Oseko F, Yamamoto T, Akamatsu Y, Kanamura N, Iwakura Y, Imanishi J, Kita MMicrobiol Immunol10.1111/j.1348-0421.2009.00123.x2009Animals, Bacteroidaceae Infections/diagnostic imaging/*immunology/microbiology/physiopathology, *Bone Resorption, Female, Gene Expression, Interleukin-17/genetics/*immunology, Male, Mice, Mice, Inbred C57BL, Mice, Inbred ICR, Mice, Knockout, Periapical Diseases/diagnostic imaging/*immunology/microbiology/physiopathology, Porphyromonas gingivalis/immunology, Prevotella intermedia/immunology, Tomography, X-Ray Computed
Pathogenicity19572892Prevotella intermedia ATCC 25611 targets host cell lamellipodia in epithelial cell adhesion and invasion.Gursoy UK, Kononen E, Uitto VJOral Microbiol Immunol10.1111/j.1399-302X.2009.00510.x2009Bacterial Adhesion, Cell Line, Cell Proliferation, Cell Survival, Epithelial Cells/*microbiology, Humans, Keratinocytes/microbiology, Prevotella intermedia/*physiology, Prevotella nigrescens/physiology, Pseudopodia/*microbiology, Skin/cytology, Species Specificity, VirulencePhylogeny
Phylogeny19654360Prevotella aurantiaca sp. nov., isolated from the human oral cavity.Sakamoto M, Suzuki N, Okamoto MInt J Syst Evol Microbiol10.1099/ijs.0.012831-02009DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/chemistry/metabolism, Humans, Molecular Sequence Data, Mouth/*microbiology, Periodontitis/microbiology, Phylogeny, Prevotella/*classification/genetics/*isolation & purification/metabolism, RNA, Ribosomal, 16S/geneticsMetabolism
Pathogenicity19708869Prevotella intermedia upregulates MMP-1 and MMP-8 expression in human periodontal ligament cells.Guan SM, Shu L, Fu SM, Liu B, Xu XL, Wu JZFEMS Microbiol Lett10.1111/j.1574-6968.2009.01748.x2009Adolescent, Blotting, Western, Cells, Cultured, Child, Enzyme-Linked Immunosorbent Assay, Gene Expression Profiling, Humans, Matrix Metalloproteinase 1/*biosynthesis, Matrix Metalloproteinase 8/*biosynthesis, Periodontal Ligament/*chemistry/*microbiology, Prevotella intermedia/*physiology, Reverse Transcriptase Polymerase Chain Reaction, Signal Transduction, *Up-Regulation
Enzymology19832801Characterization of L-cysteine desulfhydrase from Prevotella intermedia.Yano T, Fukamachi H, Yamamoto M, Igarashi TOral Microbiol Immunol10.1111/j.1399-302X.2009.00546.x2009Amino Acid Substitution, Cloning, Molecular, Cystathionine gamma-Lyase/*chemistry/*genetics/metabolism, Hemolysis/*genetics/physiology, Hydrogen Sulfide/metabolism, Mutagenesis, Site-Directed, Prevotella intermedia/*enzymology, Recombinant ProteinsMetabolism
Phylogeny19931406The identification of genes specific to Prevotella intermedia and Prevotella nigrescens using genomic subtractive hybridization.Masakiyo Y, Yoshida A, Shintani Y, Takahashi Y, Ansai T, Takehara TAnaerobe10.1016/j.anaerobe.2009.11.0032009Acyltransferases/*genetics, DNA Transposable Elements/genetics, Genes, Bacterial, Nucleic Acid Hybridization/methods, Periodontitis/microbiology, Prevotella intermedia/*genetics/pathogenicity, Prevotella nigrescens/enzymology/*genetics, Site-Specific DNA-Methyltransferase (Adenine-Specific)/genetics, Species Specificity, Virulence/geneticsEnzymology
Biotechnology20607056Leptin potentiates Prevotella intermedia lipopolysaccharide-induced production of TNF-alpha in monocyte-derived macrophages.Kim SJJ Periodontal Implant Sci10.5051/jpis.2010.40.3.1192010
Metabolism21309717Effects of luteolin on the release of nitric oxide and interleukin-6 by macrophages stimulated with lipopolysaccharide from Prevotella intermedia.Choi EY, Jin JY, Choi JI, Choi IS, Kim SJJ Periodontol10.1902/jop.2011.1007592011Animals, Anti-Inflammatory Agents/*pharmacology, Cell Line, Down-Regulation, Interleukin-6/antagonists & inhibitors/*biosynthesis, Lipopolysaccharides/*pharmacology, Luteolin/*pharmacology, MAP Kinase Signaling System, Macrophages/*drug effects/metabolism, Mice, NF-kappa B/antagonists & inhibitors/metabolism, Nitric Oxide/antagonists & inhibitors/*biosynthesis, Nitric Oxide Synthase Type II/metabolism, Phosphorylation, Prevotella intermedia/chemistry, Real-Time Polymerase Chain Reaction, STAT1 Transcription Factor/antagonists & inhibitors/metabolismPathogenicity
Enzymology21658104Molecular basis of indole production catalyzed by tryptophanase in the genus Prevotella.Sasaki-Imamura T, Yoshida Y, Suwabe K, Yoshimura F, Kato HFEMS Microbiol Lett10.1111/j.1574-6968.2011.02329.x2011Animals, Bacterial Proteins/chemistry/genetics/*metabolism, Humans, Indoles/*metabolism, Kinetics, Molecular Sequence Data, Mouth/microbiology, Phylogeny, Prevotella/classification/*enzymology/genetics/isolation & purification, Tryptophanase/chemistry/genetics/*metabolismPhylogeny
Biotechnology21811692Curcumin suppresses the production of interleukin-6 in Prevotella intermedia lipopolysaccharide-activated RAW 264.7 cells.Kim SJJ Periodontal Implant Sci10.5051/jpis.2011.41.3.1572011
Metabolism21864411Comparison of the virulence of exopolysaccharide-producing Prevotella intermedia to exopolysaccharide non-producing periodontopathic organisms.Yamanaka T, Yamane K, Furukawa T, Matsumoto-Mashimo C, Sugimori C, Nambu T, Obata N, Walker CB, Leung KP, Fukushima HBMC Infect Dis10.1186/1471-2334-11-2282011Abscess/microbiology/pathology, Animals, Immune Evasion, Male, Mice, Mice, Inbred BALB C, Phagocytosis, Polysaccharides, Bacterial/*metabolism, Porphyromonas gingivalis/immunology/*metabolism/*pathogenicity, Prevotella intermedia/immunology/*metabolism/*pathogenicity, Virulence, Virulence Factors/*metabolism
Pathogenicity21972936Anti-inflammatory effects and the underlying mechanisms of action of daidzein in murine macrophages stimulated with Prevotella intermedia lipopolysaccharide.Choi EY, Jin JY, Lee JY, Choi JI, Choi IS, Kim SJJ Periodontal Res10.1111/j.1600-0765.2011.01422.x2011Animals, Anti-Inflammatory Agents/*pharmacology, Bacteriological Techniques, Cell Culture Techniques, Cell Line, Growth Inhibitors/*pharmacology, I-kappa B Kinase/drug effects, Inflammation Mediators/antagonists & inhibitors, Interleukin-6/antagonists & inhibitors, Isoflavones/*pharmacology, JNK Mitogen-Activated Protein Kinases/drug effects, Janus Kinase 2/drug effects, Lipopolysaccharides/*pharmacology, Macrophages/*drug effects, Mice, NF-kappa B/drug effects, NF-kappa B p50 Subunit/drug effects, Nitric Oxide Synthase Type II/drug effects, Phosphorylation, Phytoestrogens/*pharmacology, *Prevotella intermedia, STAT1 Transcription Factor/drug effects, Transcription Factor RelA/drug effects, p38 Mitogen-Activated Protein Kinases/drug effects
Pathogenicity22443519Human neutrophil defensins and their effect on epithelial cells.Gursoy UK, Kononen E, Luukkonen N, Uitto VJJ Periodontol10.1902/jop.2012.1200172012Adult, Anti-Infective Agents/analysis/*pharmacology, Bacterial Adhesion/drug effects, Basement Membrane/pathology, Cell Adhesion/drug effects, Cell Death/drug effects, Cell Movement/drug effects, Cell Proliferation/drug effects, Cell Survival/drug effects, Chronic Periodontitis/pathology, Coated Materials, Biocompatible/chemistry, Dose-Response Relationship, Drug, Epithelial Attachment/drug effects/pathology, Epithelial Cells/drug effects/pathology, Female, Fibronectins/chemistry, Fusobacterium nucleatum/drug effects, Gingiva/*drug effects/pathology, Gingival Pocket/pathology, Humans, Keratinocytes/drug effects, Male, Middle Aged, Neutrophils/*physiology, Prevotella intermedia/drug effects, alpha-Defensins/analysis/*pharmacology
Cultivation22554902Contribution of hly homologs to the hemolytic activity of Prevotella intermedia.Suzuki N, Fukamachi H, Arimoto T, Yamamoto M, Igarashi TAnaerobe10.1016/j.anaerobe.2012.04.0052012Animals, Bacterial Proteins/biosynthesis/*genetics, Culture Media, Erythrocytes/microbiology/pathology, Escherichia coli, Gene Expression, Gene Expression Regulation, Bacterial, Genes, Bacterial, Hemolysin Proteins/biosynthesis/*genetics, *Hemolysis, Iron/metabolism, Prevotella intermedia/*genetics/growth & development/metabolism, Recombinant Proteins/biosynthesis/genetics, SheepMetabolism
Metabolism23266804Identification and functional analysis of the gene cluster for fructan utilization in Prevotella intermedia.Fuse H, Fukamachi H, Inoue M, Igarashi TGene10.1016/j.gene.2012.12.0232012Bacterial Proteins/genetics/metabolism, Chromosome Mapping, Culture Media, Fructans/*metabolism, Fructokinases/chemistry/genetics/metabolism, Fructose, Gene Expression, *Genes, Bacterial, Glycoside Hydrolases/genetics/metabolism, Hydrolysis, Inulin/metabolism, Monosaccharide Transport Proteins/genetics/metabolism, *Multigene Family, Prevotella intermedia/enzymology/*genetics/growth & development, Recombinant Proteins/genetics/metabolism, Sequence Analysis, DNA, Substrate SpecificityCultivation
Pathogenicity23441850Isorhamnetin inhibits Prevotella intermedia lipopolysaccharide-induced production of interleukin-6 in murine macrophages via anti-inflammatory heme oxygenase-1 induction and inhibition of nuclear factor-kappaB and signal transducer and activator of transcription 1 activation.Jin JY, Choi EY, Park HR, Choi JI, Choi IS, Kim SJJ Periodontal Res10.1111/jre.120542013Animals, Anti-Inflammatory Agents/antagonists & inhibitors/*metabolism, Antioxidants/*pharmacology, Cell Line, Down-Regulation, Enzyme Induction/drug effects, Enzyme Inhibitors/pharmacology, Heme Oxygenase-1/antagonists & inhibitors/biosynthesis/*drug effects, I-kappa B Proteins/drug effects, Interleukin-6/*antagonists & inhibitors, JNK Mitogen-Activated Protein Kinases/drug effects, Lipopolysaccharides/*antagonists & inhibitors, Macrophages/*drug effects/immunology, Membrane Proteins/antagonists & inhibitors/biosynthesis/*drug effects, Metalloporphyrins/pharmacology, Mice, NF-kappa B/*antagonists & inhibitors, NF-kappa B p50 Subunit/drug effects, Prevotella intermedia/*immunology, Protein Biosynthesis/drug effects, Protoporphyrins/pharmacology, Quercetin/*analogs & derivatives/pharmacology, STAT1 Transcription Factor/*antagonists & inhibitors, Transcription, Genetic/drug effects, Up-Regulation, p38 Mitogen-Activated Protein Kinases/drug effectsBiotechnology
Biotechnology24040572Effect of quercetin on the production of nitric oxide in murine macrophages stimulated with lipopolysaccharide from Prevotella intermedia.Cho YJ, Kim SJJ Periodontal Implant Sci10.5051/jpis.2013.43.4.1912013
Metabolism25601508Effect of caffeic acid phenethyl ester on Prevotella intermedia lipopolysaccharide-induced production of proinflammatory mediators in murine macrophages.Choi EY, Choe SH, Hyeon JY, Choi JI, Choi IS, Kim SJJ Periodontal Res10.1111/jre.122602015Animals, Caffeic Acids/*metabolism, Cytokines/*metabolism, Gene Expression Profiling, Immunologic Factors/*metabolism, Lipopolysaccharides/isolation & purification/*metabolism, Macrophages/*drug effects, Mice, NF-kappa B/metabolism, Nitric Oxide/*metabolism, Phenylethyl Alcohol/*analogs & derivatives/metabolism, Prevotella intermedia/chemistry, Real-Time Polymerase Chain ReactionPathogenicity
Phylogeny25765460Comparative genome analysis of Prevotella intermedia strain isolated from infected root canal reveals features related to pathogenicity and adaptation.Ruan Y, Shen L, Zou Y, Qi Z, Yin J, Jiang J, Guo L, He L, Chen Z, Tang Z, Qin SBMC Genomics10.1186/s12864-015-1272-32015Adult, Animals, Bacterial Proteins/genetics, Bacteriocins/metabolism, *Comparative Genomic Hybridization, Contig Mapping, DNA/chemistry/isolation & purification, Dental Pulp Cavity/*microbiology, Drug Resistance, Bacterial/genetics, Erythrocytes/metabolism, *Genome, Bacterial, Hemolysis, Humans, Iron/metabolism, Male, Models, Theoretical, Multigene Family, Open Reading Frames/genetics, Phylogeny, Prevotella intermedia/classification/*genetics/pathogenicity, Sequence Analysis, DNA, Virulence Factors/geneticsMetabolism
Pathogenicity26347298Identification of an antibacterial protein by functional screening of a human oral metagenomic library.Arivaradarajan P, Warburton PJ, Paramasamy G, Nair SP, Allan E, Mullany PFEMS Microbiol Lett10.1093/femsle/fnv1422015Aldehyde Oxidoreductases/genetics, Amino Acid Sequence, Anti-Bacterial Agents/*chemistry/isolation & purification/pharmacology, Bacillus subtilis/drug effects, Bacteria/drug effects, Bacterial Proteins/*chemistry/isolation & purification/*pharmacology, Chromosomes, Artificial, Bacterial, Cloning, Molecular, Dental Plaque/*microbiology, Escherichia coli/genetics, Escherichia coli K12/drug effects, Genetic Vectors, Genomic Library, Humans, Lactobacillus casei/drug effects, *Metagenome, Molecular Sequence Data, Mutagenesis, Neisseria/*chemistry, Plasmids, Pseudomonas aeruginosa/drug effects, Saliva/*microbiology, Sequence Analysis, DNA, Staphylococcus aureus/drug effects, Staphylococcus epidermidis/drug effectsEnzymology
Pathogenicity26406499Intraspecies Variability Affects Heterotypic Biofilms of Porphyromonas gingivalis and Prevotella intermedia: Evidences of Strain-Dependence Biofilm Modulation by Physical Contact and by Released Soluble Factors.Barbosa GM, Colombo AV, Rodrigues PH, Simionato MRPLoS One10.1371/journal.pone.01386872015Bacterial Adhesion, Biofilms/*growth & development, Biomass, Dysbiosis/microbiology, Humans, Periodontitis/microbiology, Porphyromonas gingivalis/classification/*physiology, Prevotella intermedia/classification/genetics/*physiologyPhylogeny
Pathogenicity26655950Genistein suppresses Prevotella intermedia lipopolysaccharide-induced inflammatory response in macrophages and attenuates alveolar bone loss in ligature-induced periodontitis.Choi EY, Bae SH, Ha MH, Choe SH, Hyeon JY, Choi JI, Choi IS, Kim SJArch Oral Biol10.1016/j.archoralbio.2015.11.0192015Alveolar Bone Loss/*drug therapy/genetics/metabolism, Animals, Disease Models, Animal, Gene Expression, Genistein/*pharmacology, Inflammation Mediators/*antagonists & inhibitors, Interleukin-6/antagonists & inhibitors/biosynthesis, Ligation, Lipopolysaccharides/*antagonists & inhibitors/isolation & purification/pharmacology, Macrophages/*drug effects/immunology, Male, Mice, Nitric Oxide/biosynthesis, Nitric Oxide Synthase Type II/antagonists & inhibitors/biosynthesis, Periodontitis/*drug therapy/genetics/immunology/metabolism, Prevotella intermedia/chemistry, RAW 264.7 Cells, RNA, Messenger/genetics, Random Allocation, Rats, Rats, Sprague-DawleyMetabolism
Metabolism27476978Nucleases from Prevotella intermedia can degrade neutrophil extracellular traps.Doke M, Fukamachi H, Morisaki H, Arimoto T, Kataoka H, Kuwata HMol Oral Microbiol10.1111/omi.121712016Bacteria/enzymology/genetics/pathogenicity, Bacterial Proteins/genetics/metabolism, Culture Media/chemistry, DNA/*metabolism, Deoxyribonucleases/*metabolism, Extracellular Traps/*metabolism, Fusobacterium nucleatum/enzymology/genetics/pathogenicity, Genome, Bacterial, Humans, Neutrophils/*immunology/microbiology, Porphyromonas gingivalis/enzymology/genetics/pathogenicity, Prevotella intermedia/*enzymology/genetics/pathogenicity, RNA/metabolismEnzymology
Phylogeny27722787Bacterial invasion into radicular dentine-an in vitro study.Stauffacher S, Lussi A, Nietzsche S, Neuhaus KW, Eick SClin Oral Investig10.1007/s00784-016-1960-72016Actinomyces/isolation & purification, Bacterial Load, Coculture Techniques, Dental Pulp Cavity/*microbiology, Dentin/*microbiology, Fusobacterium nucleatum/isolation & purification, Humans, In Vitro Techniques, Microscopy, Electron, Scanning, Porphyromonas gingivalis/isolation & purification, Prevotella intermedia/isolation & purification, Species Specificity, Streptococcus gordonii/isolation & purification, Streptococcus sanguis/isolation & purificationEnzymology
Pathogenicity28578027Agomelatine, a MT1/MT2 melatonergic receptor agonist with serotonin 5-HT2C receptor antagonistic properties, suppresses Prevotella intermedia lipopolysaccharide-induced production of proinflammatory mediators in murine macrophages.Hyeon JY, Choi EY, Choe SH, Park HR, Choi JI, Choi IS, Kim SJArch Oral Biol10.1016/j.archoralbio.2017.05.0152017Acetamides/*pharmacology, Animals, Cell Line, Enzyme-Linked Immunosorbent Assay, Immunoblotting, Inflammation Mediators/*antagonists & inhibitors, Interleukin-1beta/biosynthesis, Interleukin-6/biosynthesis, Lipopolysaccharides/pharmacology, Macrophages/*drug effects, Mice, NF-kappa B/biosynthesis, Nitric Oxide/biosynthesis, Nitric Oxide Synthase Type II/biosynthesis, Prevotella intermedia/*drug effects, RNA, Messenger/biosynthesis, Real-Time Polymerase Chain Reaction, STAT1 Transcription Factor/biosynthesis, STAT3 Transcription Factor/biosynthesis, Serotonin 5-HT2 Receptor Antagonists/*pharmacology, Suppressor of Cytokine Signaling 1 Protein/biosynthesisBiotechnology
Metabolism28934361Restriction-modification mediated barriers to exogenous DNA uptake and incorporation employed by Prevotella intermedia.Johnston CD, Skeete CA, Fomenkov A, Roberts RJ, Rittling SRPLoS One10.1371/journal.pone.01852342017Base Sequence, Biological Transport, Clustered Regularly Interspaced Short Palindromic Repeats/*genetics, DNA/*genetics/*metabolism, DNA Methylation, *Genomics, Humans, Prevotella intermedia/*genetics/*metabolism, Sequence Analysis, DNAGenetics
Pathogenicity30189320Quorum sensing molecules regulate epithelial cytokine response and biofilm-related virulence of three Prevotella species.Fteita D, Kononen E, Gursoy M, Ma X, Sintim HO, Gursoy UKAnaerobe10.1016/j.anaerobe.2018.09.0012018Bacteroidaceae Infections/genetics/*immunology/microbiology, Biofilms, Epithelial Cells/*immunology/microbiology, Gingiva/*immunology/microbiology, Humans, Interleukin-1beta/genetics/*immunology, Interleukin-6/genetics/*immunology, Interleukin-8/genetics/*immunology, Keratinocytes/immunology/microbiology, Prevotella/classification/genetics/pathogenicity/*physiology, Prevotella intermedia/genetics/pathogenicity/physiology, Prevotella nigrescens/genetics/pathogenicity/physiology, *Quorum SensingPhylogeny
30356383Antimicrobial Activity of Piper marginatum Jacq and Ilex guayusa Loes on Microorganisms Associated with Periodontal Disease.Gamboa F, Munoz CC, Numpaque G, Sequeda-Castaneda LG, Gutierrez SJ, Tellez NInt J Microbiol10.1155/2018/41473832018
32750671Influence of different ceramic materials and surface treatments on the adhesion of Prevotella intermedia.Poole SF, Pitondo-Silva A, Oliveira-Silva M, Moris ICM, Gomes EAJ Mech Behav Biomed Mater10.1016/j.jmbbm.2020.1040102020*Ceramics, *Computer-Aided Design, Dental Porcelain, Materials Testing, Prevotella intermedia, Surface Properties
34158697In vitro antimicrobial effect of chloroaluminum phthalocyanine nanoemulsion on periodontal bacteria.Rey LPL, Pinto SML, Muelhmann LA, Mendez LM, Sandoval LVHJ Indian Soc Periodontol10.4103/jisp.jisp_433_202021
Genetics35791444Genomic and phenotypic comparison of Prevotella intermedia strains possessing different virulence in vivo.Kwack KH, Jang EY, Yang SB, Lee JH, Moon JHVirulence10.1080/21505594.2022.20957182022Animals, Carbohydrates, *DNA Copy Number Variations, *Genomics, Heme, Iron, Mammals, Phylogeny, Prevotella intermedia/genetics, Virulence/geneticsPhylogeny

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8783Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20706)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20706
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
47910Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 24041)https://www.ccug.se/strain?id=24041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68367Automatically annotated from API 20A
68380Automatically annotated from API rID32A
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
81750Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID38613.1StrainInfo: A central database for resolving microbial strain identifiers