Strain identifier

BacDive ID: 12530

Type strain: Yes

Species: Prevotella disiens

Strain Designation: 1304-72B

Strain history: T. Mitsuoka <-- L. V. Holdeman VPI 8057 <-- D. W. Lambe.

NCBI tax ID(s): 1235811 (strain), 28130 (species)

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General

@ref: 8888

BacDive-ID: 12530

DSM-Number: 20516

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic

description: Prevotella disiens 1304-72B is an anaerobe, mesophilic bacterium that was isolated from bartholin abscess.

NCBI tax id

NCBI tax idMatching level
28130species
1235811strain

strain history

@refhistory
8888<- ATCC <- L.V. Holdeman, VPI 8057 <- D.W. Lambe Jr., 1304-72B
67770T. Mitsuoka <-- L. V. Holdeman VPI 8057 <-- D. W. Lambe.

doi: 10.13145/bacdive12530.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Bacteroidia
  • order: Bacteroidales
  • family: Prevotellaceae
  • genus: Prevotella
  • species: Prevotella disiens
  • full scientific name: Prevotella disiens (Holdeman and Johnson 1977) Shah and Collins 1990

  • synonyms

    • @ref: 20215
    • synonym: Bacteroides disiens

@ref: 8888

domain: Bacteria

phylum: Bacteroidetes

class: Bacteroidia

order: Bacteroidales

family: Prevotellaceae

genus: Prevotella

species: Prevotella disiens

full scientific name: Prevotella disiens (Holdeman and Johnson 1977) Shah and Collins 1990

strain designation: 1304-72B

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram stain
69480no93.974
6948099.995negative

colony morphology

  • @ref: 45194
  • incubation period: 2 days

Culture and growth conditions

culture medium

  • @ref: 8888
  • name: PYG MEDIUM (MODIFIED) (DSMZ Medium 104)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/104
  • composition: Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water

culture temp

@refgrowthtypetemperaturerange
8888positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
8888anaerobe
45194anaerobe
69480anaerobe99.999

spore formation

@refspore formationconfidence
69481no100
69480no100

observation

  • @ref: 67770
  • observation: quinones: MK-11 (MK-12)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68380serine arylamidase-
68380glutamyl-glutamate arylamidase+
68380histidine arylamidase-
68380glycin arylamidase+
68380alanine arylamidase+3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase+3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase+
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase+3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase-3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
8888-----+----------+/-+-+----++-+-

Isolation, sampling and environmental information

isolation

@refsample type
8888bartholin abscess
45194Bartholin abscess
67770Bartholin abscess (leukemia)

isolation source categories

Cat1Cat2Cat3
#Infection#Disease
#Host#Human#Female
#Host Body-Site#Other#Abscess
#Host Body-Site#Urogenital tract#Vagina

taxonmaps

  • @ref: 69479
  • File name: preview.99_3328.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_295;96_1735;97_2076;98_2552;99_3328&stattab=map
  • Last taxonomy: Prevotella disiens subclade
  • 16S sequence: AB547682
  • Sequence Identity:
  • Total samples: 57720
  • soil counts: 815
  • aquatic counts: 844
  • animal counts: 55740
  • plant counts: 321

Safety information

risk assessment

  • @ref: 8888
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Prevotella disiens ATCC 29426 16S ribosomal RNA gene, complete sequenceL164831479ena28130
20218Prevotella disiens gene for 16S ribosomal RNA, partial sequence, strain: JCM 6334AB5476821492ena28130

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Prevotella disiens JCM 6334 = ATCC 294261235811.4wgspatric1235811
66792Prevotella disiens JCM 6334 = ATCC 294261235811.5wgspatric1235811
66792Prevotella disiens strain NCTC1115728130.5wgspatric28130
66792Prevotella disiens NCTC 111572871815117draftimg28130
66792Prevotella disiens VPI 80572585427922draftimg1235811
66792Prevotella disiens VPI 80572600254990draftimg1235811
67770Prevotella disiens JCM 6334 = ATCC 29426GCA_000467875scaffoldncbi1235811
67770Prevotella disiens JCM 6334 = ATCC 29426GCA_000613345contigncbi1235811
67770Prevotella disiens NCTC11157GCA_900454955contigncbi28130

GC content

@refGC-contentmethod
888840.0
6777040thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno93.954no
gram-positiveno97.752no
anaerobicyes98.431yes
aerobicno98.281yes
halophileno83.474no
spore-formingno97.312no
glucose-utilyes80.608no
flagellatedno97.394no
thermophileno97.053no
glucose-fermentyes51.195no

External links

@ref: 8888

culture collection no.: CCUG 9558, JCM 6334, LMG 6453, CIP 105108, DSM 20516, ATCC 29426, NCTC 11157, VPI 8057

straininfo link

  • @ref: 81749
  • straininfo: 3863

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8888Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20516)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20516
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
45194Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 9558)https://www.ccug.se/strain?id=9558
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68380Automatically annotated from API rID32A
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
81749Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID3863.1StrainInfo: A central database for resolving microbial strain identifiers