Strain identifier

BacDive ID: 1253

Type strain: Yes

Species: Bacillus seohaeanensis

Strain Designation: BH 724, 6.101, BH724

Strain history: CIP <- 2009, DSMZ <- C. J. Kim <- D. J. Park., Yusong Korea Res. Inst. Bioscience & Biotechnology, Daejeon, Korea: strain BH724

NCBI tax ID(s): 284580 (species)

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General

@ref: 6455

BacDive-ID: 1253

DSM-Number: 16464

keywords: 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive, rod-shaped

description: Bacillus seohaeanensis BH 724 is an aerobe, spore-forming, mesophilic bacterium that was isolated from salt lake soil.

NCBI tax id

  • NCBI tax id: 284580
  • Matching level: species

strain history

@refhistory
6455<- C.-J. Kim <- D.-J. Park
67771<- CJ Kim, KRIBB
122559CIP <- 2009, DSMZ <- C. J. Kim <- D. J. Park., Yusong Korea Res. Inst. Bioscience & Biotechnology, Daejeon, Korea: strain BH724

doi: 10.13145/bacdive1253.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Bacillus
  • species: Bacillus seohaeanensis
  • full scientific name: Bacillus seohaeanensis Lee et al. 2006

@ref: 6455

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Bacillus

species: Bacillus seohaeanensis

full scientific name: Bacillus seohaeanensis Lee et al. 2006

strain designation: BH 724, 6.101, BH724

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
31769positive1.3-1.8 µm0.5-0.6 µmrod-shapedno
67771rod-shapedno
67771positive
122559positiverod-shapedno

pigmentation

  • @ref: 31769
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6455BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514; with strain-specific modifications) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l MnSO4 0.01 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
37907Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
122559CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperaturerange
6455positivegrowth30mesophilic
31769positivegrowth15-50
31769positiveoptimum35-40
37907positivegrowth37mesophilic
67771positivegrowth34mesophilic

culture pH

@refabilitytypepH
31769positivegrowth05-08
31769positiveoptimum7.5

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
31769aerobe
67771aerobe
122559facultative anaerobe

spore formation

@refspore formationtype of spore
31769yes
67771yesendospore

halophily

@refsaltgrowthtested relationconcentration
31769NaClpositivegrowth0-10 %
31769NaClpositiveoptimum03-05 %

observation

@refobservation
31769aggregates in chains
67771quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3176928757fructose+carbon source
3176917234glucose+carbon source
3176917754glycerol+carbon source
3176917306maltose+carbon source
3176929864mannitol+carbon source
3176937684mannose+carbon source
3176933942ribose+carbon source
3176927082trehalose+carbon source
3176918222xylose+carbon source
317694853esculin+hydrolysis
12255917632nitrate+reduction
12255916301nitrite-reduction

metabolite production

  • @ref: 122559
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
31769catalase+1.11.1.6
31769cytochrome oxidase+1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
122559oxidase+
122559catalase+1.11.1.6
122559urease+3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122559-+++----------------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
6455salt lake soilChinaCHNAsia
67771From salt lake soilChinaCHNAsia
122559Environment, Soil, salt lakeChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Lake (large)
#Environmental#Terrestrial#Soil
#Condition#Saline

taxonmaps

  • @ref: 69479
  • File name: preview.99_67889.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_1652;97_36964;98_47830;99_67889&stattab=map
  • Last taxonomy: Bacillus
  • 16S sequence: AY667495
  • Sequence Identity:
  • Total samples: 545
  • soil counts: 215
  • aquatic counts: 154
  • animal counts: 137
  • plant counts: 39

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
64551Risk group (German classification)
1225591Risk group (French classification)

Sequence information

16S sequences

  • @ref: 6455
  • description: Bacillus seohaeanensis strain BH724 16S ribosomal RNA gene, partial sequence
  • accession: AY667495
  • length: 1401
  • database: ena
  • NCBI tax ID: 284580

GC content

@refGC-content
645539
6777139.0

External links

@ref: 6455

culture collection no.: DSM 16464, CIP 110058, KCTC 3913

straininfo link

  • @ref: 70909
  • straininfo: 232133

literature

  • topic: Phylogeny
  • Pubmed-ID: 16902027
  • title: Bacillus seohaeanensis sp. nov., a halotolerant bacterium that contains L-lysine in its cell wall.
  • authors: Lee JC, Lim JM, Park DJ, Jeon CO, Li WJ, Kim CJ
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.64237-0
  • year: 2006
  • mesh: Bacillus/chemistry/*classification/isolation & purification/physiology, Base Composition, Cell Wall/chemistry, Culture Media, Cyanoacrylates/analysis, DNA, Bacterial/genetics, Fatty Acids/analysis, *Industrial Microbiology, Lysine/analysis/metabolism, Molecular Sequence Data, Peptidoglycan/analysis/metabolism, Phenotype, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Homology, Nucleic Acid, Sodium Chloride, Species Specificity, Temperature, Vitamin K 2/analogs & derivatives
  • topic2: Cultivation

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
6455Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16464)https://www.dsmz.de/collection/catalogue/details/culture/DSM-16464
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
31769Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2804228776041
37907Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7873
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
70909Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID232133.1StrainInfo: A central database for resolving microbial strain identifiers
122559Curators of the CIPCollection of Institut Pasteur (CIP 110058)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110058