Strain identifier

BacDive ID: 1252

Type strain: Yes

Species: Halalkalibacter krulwichiae

Strain Designation: AM31D

Strain history: I. Yumoto AM31D.

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 7441

BacDive-ID: 1252

DSM-Number: 18225

keywords: mesophilic, Bacteria, 16S sequence, genome sequence

description: Halalkalibacter krulwichiae AM31D is a mesophilic bacterium that was isolated from soil.

NCBI tax id

NCBI tax idMatching level
199441species
1220588strain

strain history

doi: 10.13145/bacdive1252.20220920.7

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Halalkalibacter
  • species: Halalkalibacter krulwichiae
  • full scientific name: Halalkalibacter krulwichiae (Yumoto et al. 2003) Joshi et al. 2022
  • synonyms

    @refsynonym
    20215Alkalihalobacillus krulwichiae
    20215Bacillus krulwichiae

@ref: 7441

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Alkalihalobacillus

species: Alkalihalobacillus krulwichiae

full scientific name: Alkalihalobacillus krulwichiae (Yumoto et al. 2003) Patel and Gupta 2020

strain designation: AM31D

type strain: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7441ALKALINE NUTRIENT AGAR (DSMZ Medium 31)yeshttps://bacmedia.dsmz.de/medium/31Name: ALKALINE NUTRIENT AGAR (DSMZ Medium 31) Composition: Agar 15.0 g/l Na2CO3 anhydrous 5.3 g/l Peptone 5.0 g/l NaHCO3 4.2 g/l Meat extract 3.0 g/l Distilled water
32895MEDIUM 291 - for Bacillus hortiyesDistilled water make up to (900.000 ml);Magnesium sulphate heptahydrate (0.200 g);Agar (15.000 g);Glucose (10.000 g);Yeast extract (5.000 g);Potassium di-hydrogen phosphate (1.000 g);Polypeptone (5.000 g);Sodium carbonate solution - M00442 (100.000 ml)

culture temp

@refgrowthtypetemperaturerange
7441positivegrowth30mesophilic
32895positivegrowth30mesophilic
67770positivegrowth27mesophilic

Physiology and metabolism

observation

  • @ref: 67770
  • observation: quinones: MK-7, MK-6, MK-5

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
7441soilIbaraki, TsukubaJapanJPNAsia
67770Soil sample obtained from TsukubaIbaraki Pref.JapanJPNAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

  • @ref: 7441
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
7441Bacillus krulwichiae gene for 16S rRNA, partial sequenceAB0868971507ena199441
67770Bacillus krulwichiae gene for 16S rRNA, partial sequence, strain: NBRC 102362AB6817541412ena199441

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Alkalihalobacillus krulwichiae NBRC 102362 NBRC 102362GCA_001591945contigncbi1220588
66792Alkalihalobacillus krulwichiae AM31DGCA_002109385completencbi199441
66792Bacillus krulwichiae NBRC 1023621220588.3wgspatric1220588
66792Bacillus krulwichiae NBRC 1023622731957674draftimg1220588
66792Bacillus krulwichiae NBRC 1023622773857802completeimg1220588
67770Alkalihalobacillus krulwichiae NBRC 102362, whole genome shotgun sequencing projectBCVW00000000ncbi1220588
67770Alkalihalobacillus krulwichiae strain AM31D chromosome, complete genomeCP020814ena199441

GC content

@refGC-contentmethod
744141.5
6777041.5high performance liquid chromatography (HPLC)

External links

@ref: 7441

culture collection no.: DSM 18225, IAM 15000, JCM 11691, NCIMB 13904, CIP 108137, NBRC 102362

straininfo link

@refpassport
20218http://www.straininfo.net/strains/691299
20218http://www.straininfo.net/strains/371201
20218http://www.straininfo.net/strains/371202
20218http://www.straininfo.net/strains/371203

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny13130043Bacillus krulwichiae sp. nov., a halotolerant obligate alkaliphile that utilizes benzoate and m-hydroxybenzoate.Yumoto I, Yamaga S, Sogabe Y, Nodasaka Y, Matsuyama H, Nakajima K, Suemori AInt J Syst Evol Microbiol10.1099/ijs.0.02596-02003Bacillus/*classification/genetics/isolation & purification/*metabolism, Benzoates/metabolism, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/analysis, Hydroxybenzoates/metabolism, Japan, Microscopy, Electron, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sodium Chloride, Soil Microbiology, Vitamin K 2/analysisMetabolism
Phylogeny20833886Bacillus alkalisediminis sp. nov., an alkaliphilic and moderately halophilic bacterium isolated from sediment of extremely shallow soda ponds.Borsodi AK, Pollak B, Keki Z, Rusznyak A, Kovacs AL, Sproer C, Schumann P, Marialigeti K, Toth EMInt J Syst Evol Microbiol10.1099/ijs.0.019489-02010Alkalies/*metabolism, Bacillus/*classification/genetics/*isolation & purification/metabolism, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Geologic Sediments/*microbiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sodium Chloride/*metabolism, *Water MicrobiologyMetabolism
Metabolism31126948A Pathway for Isethionate Dissimilation in Bacillus krulwichiae.Tong Y, Wei Y, Hu Y, Ang EL, Zhao H, Zhang YAppl Environ Microbiol10.1128/AEM.00793-192019Acetaldehyde/*analogs & derivatives/metabolism, Acetyltransferases/*genetics, Bacillus/*metabolism, Bacterial Proteins/*genetics, Isethionic Acid/*metabolism, Multigene Family
Enzymology34021568Molecular and biochemical characteristics of inulosucrase InuBK from Alkalihalobacillus krulwichiae JCM 11691.Yokoi KJ, Tsutsui S, Arakawa GY, Takaba M, Fujii K, Kaneko SBiosci Biotechnol Biochem10.1093/bbb/zbab0942021Bacillaceae/*enzymology/genetics, Chromatography, High Pressure Liquid/methods, Cloning, Molecular, Culture Media, Genes, Bacterial, Hexosyltransferases/genetics/isolation & purification/*metabolism, Inulin/biosynthesis, Magnetic Resonance Spectroscopy/methods, Molecular Weight, Phylogeny, TemperaturePhylogeny

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
7441Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18225)https://www.dsmz.de/collection/catalogue/details/culture/DSM-18225
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
32895Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5726
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/