Strain identifier

BacDive ID: 1251

Type strain: Yes

Species: Neobacillus fumarioli

Strain Designation: B1801T

Strain history: CIP <- 2001, NA. Logan, Caledonian Univ., Glasgow, UK: strain B1801T

NCBI tax ID(s): 1314754 (strain), 105229 (species)

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General

@ref: 7449

BacDive-ID: 1251

DSM-Number: 18237

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, thermophilic, Gram-positive, motile, rod-shaped, colony-forming

description: Neobacillus fumarioli B1801T is an aerobe, spore-forming, thermophilic bacterium that forms circular colonies and was isolated from soil, active fumarole.

NCBI tax id

NCBI tax idMatching level
1314754strain
105229species

strain history

@refhistory
7449<- NCIMB
386542001, N.A. Logan, Caledonian Univ., Glasgow, UK: strain B1801T
67770IAM 15257 <-- LMG 19448 <-- N. Logan B1801 (=strain Rcp Sm1).
119489CIP <- 2001, NA. Logan, Caledonian Univ., Glasgow, UK: strain B1801T

doi: 10.13145/bacdive1251.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Neobacillus
  • species: Neobacillus fumarioli
  • full scientific name: Neobacillus fumarioli (Logan et al. 2000) Patel and Gupta 2020

  • synonyms

    • @ref: 20215
    • synonym: Bacillus fumarioli

@ref: 7449

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Neobacillus

species: Neobacillus fumarioli

full scientific name: Neobacillus fumarioli (Logan et al. 2000) Patel and Gupta 2020

strain designation: B1801T

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
23318positive4-8 µm0.5-0.8 µmrod-shapedyes
69480yes92.823
69480positive100
119489positiverod-shapedno

colony morphology

@refcolony sizecolony colorcolony shapeincubation periodmedium used
233185-10 mmbrownish-cream, opaquecircular2-3 daysTSA
233183-8 mmcreamy browncircular2-3 daysBFA

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7449ALICYCLOBACILLUS MEDIUM (DSMZ Medium 402)yeshttps://mediadive.dsmz.de/medium/402Name: ALICYCLOBACILLUS MEDIUM (DSMZ Medium 402) Composition: Agar 15.0 g/l Glucose 5.0 g/l KH2PO4 3.0 g/l Yeast extract 2.0 g/l MgSO4 x 7 H2O 0.5 g/l CaCl2 x 2 H2O 0.25 g/l (NH4)2SO4 0.2 g/l H3BO3 0.0003 g/l CoCl2 x 6 H2O 0.0002 g/l ZnSO4 x 7 H2O 0.0001 g/l MnCl2 x 4 H2O 3e-05 g/l Na2MoO4 x 2 H2O 3e-05 g/l NiCl2 x 6 H2O 2e-05 g/l CuCl2 x 2 H2O 1e-05 g/l Distilled water
23318BFAyes
23318Trypticase Soy Agar (TSA)yes
38654MEDIUM 371 - for Bacillus fumarioliyesDistilled water make up to (992.000 ml);Agar (18.000 g);Yeast extract (4.000 g);Ammonium sulphate (2.000 g);Potassium di-hydrogen phosphate (3.000 g);Solution A- M00715 (4.000 ml);Solution B - M00715 (4.000 ml)
119489CIP Medium 113yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=113

culture temp

@refgrowthtypetemperaturerange
7449positivegrowth45thermophilic
23318positivemaximum55thermophilic
23318positiveminimum25-30mesophilic
23318positiveoptimum50thermophilic
38654positivegrowth50thermophilic
67770positivegrowth52thermophilic
119489positivegrowth37-45
119489nogrowth10psychrophilic
119489nogrowth22psychrophilic
119489nogrowth30mesophilic

culture pH

@refabilitytypepHPH range
23318positiveminimum4.0-5.0acidophile
23318positiveoptimum5.5
23318positivemaximum6.0-6.5

Physiology and metabolism

oxygen tolerance

  • @ref: 23318
  • oxygen tolerance: aerobe

spore formation

@refspore descriptiontype of sporespore formationconfidence
23318ellipsoidal to cylindrical, paracentrally and subterminally, unswollen sporangiaendosporeyes
69481yes100
69480yes100

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2331816947citrate-assimilation
23318168082-dehydro-D-gluconate-builds acid from
23318174265-dehydro-D-gluconate-builds acid from
2331815963ribitol-builds acid from
2331827613amygdalin-builds acid from
2331818305arbutin-builds acid from
2331817057cellobiose-builds acid from
2331817108D-arabinose-builds acid from
2331818333D-arabitol-builds acid from
2331828847D-fucose-builds acid from
2331862318D-lyxose-builds acid from
2331828053melibiose-builds acid from
2331816443D-tagatose-builds acid from
2331865327D-xylose-builds acid from
2331816813galactitol-builds acid from
2331817113erythritol-builds acid from
2331828260galactose-builds acid from
2331828066gentiobiose-builds acid from
2331824265gluconate-builds acid from
2331828087glycogen-builds acid from
2331815443inulin-builds acid from
2331830849L-arabinose-builds acid from
2331818403L-arabitol-builds acid from
2331818287L-fucose-builds acid from
2331817266L-sorbose-builds acid from
2331865328L-xylose-builds acid from
2331817716lactose-builds acid from
2331817268myo-inositol-builds acid from
23318320061methyl alpha-D-glucopyranoside-builds acid from
2331843943methyl alpha-D-mannoside-builds acid from
2331826546rhamnose-builds acid from
2331833942ribose-builds acid from
2331817814salicin-builds acid from
2331830911sorbitol-builds acid from
2331828017starch-builds acid from
2331817151xylitol-builds acid from
23318casein-hydrolysis
23318O-nitrophenyl-beta-D-galactopyranosid-hydrolysis
2331817632nitrate-reduction
2331815824D-fructose+builds acid from
2331817634D-glucose+builds acid from
2331816024D-mannose+builds acid from
233186731melezitose+builds acid from
2331816634raffinose+builds acid from
2331832528turanose+builds acid from
2331817754glycerol+builds acid from
2331817306maltose+builds acid from
2331829864mannitol+builds acid from
23318506227N-acetylglucosamine+builds acid from
2331817992sucrose+builds acid from
2331827082trehalose+builds acid from
233185291gelatin+hydrolysis
233184853esculin+/-hydrolysis
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837116024D-mannose-builds acid from
6837115824D-fructose-builds acid from
6837117634D-glucose-builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
1194894853esculin-hydrolysis
11948917632nitrate-reduction
11948916301nitrite-reduction

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantresistance conc.is sensitivesensitivity conc.
23318100147nalidixic acidyesyes30 µg
2331837943colistinyesyes100 µg
2331827902tetracyclineyesyes100 µg
2331828971ampicillinyesyes25 µg
2331817076streptomycinyesyes25 µg
233186104kanamycinyesyes30 µg
2331817698chloramphenicolyesyes50 µg
2331871415nitrofurantoinyesyes50 µg

metabolite production

@refChebi-IDmetaboliteproduction
2331835581indoleno
2331816136hydrogen sulfideno
2331815688acetoinyes
11948935581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
2331815688acetoin+
11948915688acetoin-
11948917234glucose-

enzymes

@refvalueactivityec
23318arginine dihydrolase-3.5.3.6
23318catalase+1.11.1.6
23318lysine decarboxylase-4.1.1.18
23318ornithine decarboxylase-4.1.1.17
23318tryptophan deaminase-4.1.99.1
23318urease-3.5.1.5
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase+3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
119489oxidase-
119489beta-galactosidase-3.2.1.23
119489alcohol dehydrogenase-1.1.1.1
119489gelatinase+/-
119489amylase-
119489DNase-
119489caseinase-3.4.21.50
119489catalase+1.11.1.6
119489tween esterase-
119489lecithinase-
119489lipase-
119489lysine decarboxylase-4.1.1.18
119489ornithine decarboxylase-4.1.1.17
119489urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119489--++-------++-+-----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
119489----+/---------+/-----------+/-----------------+/-------+/-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119489--------------------------------------------------------------+--+--------------------+------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcontinentcountryorigin.countryisolation date
7449soil, active fumaroleMT RittmannAustralia and Oceania
67770Soil from active fumaroleMt Rittmann in northern Victoria LandAntarcticaAntarcticaATA
119489Environment, Geothermal soilMount Rittmann, Victoria landAntarcticaAntarcticaATA1997

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Environmental#Terrestrial#Volcanic

taxonmaps

  • @ref: 69479
  • File name: preview.99_6616.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_292;97_323;98_4878;99_6616&stattab=map
  • Last taxonomy: Neobacillus fumarioli
  • 16S sequence: AJ250056
  • Sequence Identity:
  • Total samples: 374
  • soil counts: 295
  • aquatic counts: 17
  • animal counts: 51
  • plant counts: 11

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
74491Risk group (German classification)
1194891Risk group (French classification)

Sequence information

16S sequences

  • @ref: 7449
  • description: Bacillus fumarioli 16S rRNA gene, strain LMG 17489
  • accession: AJ250056
  • length: 1466
  • database: ena
  • NCBI tax ID: 105229

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Bacillus fumarioli NBRC 1024281314754.3wgspatric1314754
66792Bacillus fumarioli NBRC 1024282731957653draftimg1314754
67770Neobacillus fumarioli NBRC 102428GCA_001591485contigncbi1314754

GC content

@refGC-contentmethod
744940.7
2331840.7high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileyes79.445no
gram-positiveyes84.463yes
anaerobicno97.566no
halophileno94.862no
spore-formingyes97.128yes
glucose-utilyes93.327no
flagellatedyes61.261no
thermophileno50no
aerobicyes87.766yes
glucose-fermentno89.479no

External links

@ref: 7449

culture collection no.: DSM 18237, LMG 17489, NCIMB 13771, Rcp Sm1, JCM 21708, CIP 106910, IAM 15257, KCTC 13885, KCTC 3851, LMG 19448, NBRC 102428

straininfo link

  • @ref: 70907
  • straininfo: 12034

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny11034482Aerobic endospore-forming bacteria from geothermal environments in northern Victoria Land, Antarctica, and Candlemas Island, South Sandwich archipelago, with the proposal of Bacillus fumarioli sp. nov.Logan NA, Lebbe L, Hoste B, Goris J, Forsyth G, Heyndrickx M, Murray BL, Syme N, Wynn-Williams DD, De Vos PInt J Syst Evol Microbiol10.1099/00207713-50-5-17412000Antarctic Regions, Bacillus/*classification/genetics/*isolation & purification/physiology, Bacterial Proteins/analysis/chemistry, Base Composition, Culture Media, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/analysis/genetics, Electrophoresis, Polyacrylamide Gel, Fatty Acids/analysis, Hydrogen-Ion Concentration, Molecular Sequence Data, Nucleic Acid Hybridization/methods, Phenotype, RNA, Ribosomal, 16S/genetics, Restriction Mapping, Sequence Analysis, DNA, *Soil Microbiology, *Volcanic EruptionsEnzymology
Phylogeny11732714Isolation of a bacterium from mangrove soil for degradation of sea sludge.Ando Y, Mitsugi N, Yano K, Karube IAppl Biochem Biotechnol10.1385/abab:95:3:1752001Bacteria/classification/genetics/*isolation & purification/metabolism, Carbon Dioxide/metabolism, DNA, Bacterial/analysis, Fermentation, Molecular Sequence Data, Oceans and Seas, Phylogeny, *Sewage, *Soil Microbiology, TreesMetabolism
Phylogeny22611202Bacillus ginsengisoli sp. nov., isolated from soil of a ginseng field.Nguyen NL, Kim YJ, Hoang VA, Min JW, Liang ZQ, Yang DCInt J Syst Evol Microbiol10.1099/ijs.0.031740-02012Bacillus/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Molecular Sequence Data, Panax/*microbiology, *Phylogeny, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, *Soil MicrobiologyGenetics
Phylogeny24908072Bacillus thermophilum sp. nov., isolated from a microbial fuel cell.Tang J, Yang G, Wen J, Yu Z, Zhou S, Liu ZArch Microbiol10.1007/s00203-014-1001-32014Bacillus/chemistry/classification/*isolation & purification/*physiology, Bacterial Typing Techniques, Base Composition, Bioelectric Energy Sources/*microbiology, *Biofilms, Fatty Acids/analysis, Molecular Sequence Data, Phosphatidylethanolamines, Phospholipids/analysis/genetics, *Phylogeny, RNA, Ribosomal, 16S/genetics, Species SpecificityGenetics
Phylogeny33620309Neobacillus endophyticus sp. nov., an endophytic bacterium isolated from Selaginella involvens roots.Jiang L, Lee MH, Jeong JC, Kim DH, Kim CY, Kim SW, Lee JInt J Syst Evol Microbiol10.1099/ijsem.0.0045812021

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
7449Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18237)https://www.dsmz.de/collection/catalogue/details/culture/DSM-18237
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23318N A Logan, L Lebbe, B Hoste, J Goris, G Forsyth, M Heyndrickx, B L Murray, N Syme, D D Wynn-Williams, P De Vos10.1099/00207713-50-5-1741Aerobic endospore-forming bacteria from geothermal environments in northern Victoria Land, Antarctica, and Candlemas Island, South Sandwich archipelago, with the proposal of Bacillus fumarioli sp. nov.IJSEM 50: 1741-1753 200011034482
38654Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/19093
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
70907Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID12034.1StrainInfo: A central database for resolving microbial strain identifiers
119489Curators of the CIPCollection of Institut Pasteur (CIP 106910)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106910