Strain identifier

BacDive ID: 1250

Type strain: Yes

Species: Mesobacillus jeotgali

Strain Designation: YKJ-10

Strain history: CIP <- 2001, JCM <- Y-H., Park: strain YKJ-10

NCBI tax ID(s): 129985 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 9.3 (current version):
version 9.2:
version 9.1:
version 9:
version 8.1:
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 9.3 (current version)

General

@ref: 7442

BacDive-ID: 1250

DSM-Number: 18226

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, spore-forming, mesophilic, Gram-variable, motile, rod-shaped, colony-forming

description: Mesobacillus jeotgali YKJ-10 is a facultative anaerobe, spore-forming, mesophilic bacterium that forms irregular colonies and was isolated from jeotgal, Korean traditional fermented seafood.

NCBI tax id

  • NCBI tax id: 129985
  • Matching level: species

strain history

@refhistory
7442<- CIP
67770Y.-H. Park YKJ-10.
120344CIP <- 2001, JCM <- Y-H., Park: strain YKJ-10

doi: 10.13145/bacdive1250.20250331.9.3

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Mesobacillus
  • species: Mesobacillus jeotgali
  • full scientific name: Mesobacillus jeotgali (Yoon et al. 2001) Patel and Gupta 2020

  • synonyms

    • @ref: 20215
    • synonym: Bacillus jeotgali

@ref: 7442

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Mesobacillus

species: Mesobacillus jeotgali

full scientific name: Mesobacillus jeotgali (Yoon et al. 2001) Patel and Gupta 2020

strain designation: YKJ-10

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityflagellum arrangement
23317variable4.0-6.0 µm0.8-1.1 µmrod-shapedyesperitrichous
120344positiverod-shapedyes

colony morphology

@refcolony colorcolony shapeincubation periodmedium usedhemolysis ability
23317cream-yellowirregular2-3 daysTSA+ASW
1203441

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7442BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
23317TSA+ASWyes
39660Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
120344CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperature
7442positivegrowth30
23317inconsistentgrowth50
23317nogrowth55
23317positivemaximum45
23317positiveminimum10
23317positiveoptimum30-35
39660positivegrowth30
67770positivegrowth30
120344positivegrowth10-45
120344nogrowth55

culture pH

@refabilitytypepH
23317positiveoptimum7.0-8.0
23317nogrowth<5.0
120344positivegrowth6

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
23317facultative anaerobe
120344facultative anaerobe

spore formation

  • @ref: 125438
  • spore formation: yes
  • confidence: 90.689

halophily

@refsaltgrowthtested relationconcentration
23317NaClpositivegrowth13 %
23317NaClnogrowth14 %
120344NaClpositivegrowth0-8 %
120344NaClnogrowth10 %

observation

  • @ref: 67770
  • observation: quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2331724265gluconate-builds acid from
2331753426tween 80-degradation
23317174265-dehydro-D-gluconate+builds acid from
2331717057cellobiose+builds acid from
2331728066gentiobiose+builds acid from
2331717992sucrose+builds acid from
12034416947citrate-carbon source
1203444853esculin+hydrolysis
120344606565hippurate-hydrolysis
12034417632nitrate+reduction
12034416301nitrite+reduction
12034417632nitrate+respiration
68371Potassium 5-ketogluconate-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen+builds acid from
6837128017starch+builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose+builds acid from
6837117992sucrose+builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose+builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from

metabolite production

  • @ref: 120344
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12034415688acetoin+
12034417234glucose+

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382beta-glucosidase-3.2.1.21
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382trypsin-3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382cystine arylamidase+3.4.11.3
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382alkaline phosphatase+3.1.3.1
120344oxidase+
120344beta-galactosidase-3.2.1.23
120344alcohol dehydrogenase-1.1.1.1
120344gelatinase+
120344amylase-
120344DNase+
120344caseinase+3.4.21.50
120344catalase+1.11.1.6
120344tween esterase+
120344gamma-glutamyltransferase+2.3.2.2
120344lecithinase-
120344lipase-
120344lysine decarboxylase-4.1.1.18
120344ornithine decarboxylase-4.1.1.17
120344urease-3.5.1.5
68382valine arylamidase+

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120344-+++-+++-+++---+----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
120344----------+++--------+/---+/---+--++---++-+/-----------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120344-------------------------------+------------------------------+--+--------------------+------------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
7442jeotgal, Korean traditional fermented seafoodRepublic of KoreaKORAsia
67770Jeotgal, Korean traditional fermented seafoodRepublic of KoreaKORAsia
120344Food, Korean traditonal fermented foodRepublic of KoreaKORAsia

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Seafood
#Engineered#Food production#Fermented

taxonmaps

  • @ref: 69479
  • File name: preview.99_2877.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_292;97_834;98_2226;99_2877&stattab=map
  • Last taxonomy: Mesobacillus
  • 16S sequence: AF221061
  • Sequence Identity:
  • Total samples: 2499
  • soil counts: 1008
  • aquatic counts: 793
  • animal counts: 580
  • plant counts: 118

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
74421Risk group (German classification)
1203441Risk group (French classification)

Sequence information

16S sequences

  • @ref: 7442
  • description: Bacillus sp. YKJ-10 16S ribosomal RNA gene, partial sequence
  • accession: AF221061
  • length: 1505
  • database: nuccore
  • NCBI tax ID: 129985

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mesobacillus jeotgali DSM 18226GCA_002874535chromosomencbi129985
66792Bacillus jeotgali strain DSM 18226129985.4completepatric129985
66792Bacillus jeotgali DSM 182262908928733completeimg129985

GC content

@refGC-contentmethod
744241
2331741.00high performance liquid chromatography (HPLC)
6777041high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

@refmodeltraitdescriptionpredictionconfidencetraining_data
125438gram-positivegram-positivePositive reaction to Gram-stainingyes77.175no
125438anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no88.998yes
125438spore-formingspore-formingAbility to form endo- or exosporesyes90.689no
125438aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes51.38yes
125438thermophilethermophilicAbility to grow at temperatures above or equal to 45°Cno92.402yes
125438motile2+flagellatedAbility to perform flagellated movementyes81.926yes
125439BacteriaNetspore_formationAbility to form endo- or exosporesyes89.2
125439BacteriaNetmotilityAbility to perform movementyes85.1
125439BacteriaNetgram_stainReaction to gram-stainingvariable82.3
125439BacteriaNetoxygen_toleranceOxygenic conditions needed for growthobligate aerobe82.2

External links

@ref: 7442

culture collection no.: DSM 18226, CIP 107104, JCM 10885, KCCM 41040, BCRC 20126, CCM 7133, CGMCC 1.3651, KCTC 3839

straininfo link

  • @ref: 70906
  • straininfo: 88941

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny11411677Bacillus jeotgali sp. nov., isolated from jeotgal, Korean traditional fermented seafood.Yoon JH, Kang SS, Lee KC, Kho YH, Choi SH, Kang KH, Park YHInt J Syst Evol Microbiol10.1099/00207713-51-3-10872001Bacillus/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/analysis, Fermentation, Food Handling, Korea, Molecular Sequence Data, Phenotype, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seafood/*microbiology, Species SpecificityGenetics
Metabolism15747087Degradation of ferric chelate of ethylenediaminetetraacetic acid by bacterium isolated from deep-sea stalked barnacle.Imada C, Harada Y, Kobayashi T, Hamada-Sato N, Watanabe EMar Biotechnol (NY)10.1007/s10126-004-3700-22005Animals, Bacillus/*metabolism, Biodegradation, Environmental, Chromatography, Ion Exchange, Edetic Acid/metabolism, Ferric Compounds/*metabolism, Indian Ocean, Iron Chelating Agents/*metabolism, Thoracica/*microbiology, Water Pollutants, Chemical/*metabolism
Phylogeny17451444Isolation and characterization of Bacillus thioparus sp. nov., chemolithoautotrophic, thiosulfate-oxidizing bacterium.Perez-Ibarra BM, Flores ME, Garcia-Varela MFEMS Microbiol Lett10.1111/j.1574-6968.2007.00729.x2007Bacillus/*genetics/isolation & purification/*metabolism, DNA, Bacterial/chemistry/genetics, Molecular Sequence Data, Oxidation-Reduction, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sulfates/chemistry/metabolism, Thiosulfates/chemistry/*metabolismMetabolism
Phylogeny17473259Bacillus selenatarsenatis sp. nov., a selenate- and arsenate-reducing bacterium isolated from the effluent drain of a glass-manufacturing plant.Yamamura S, Yamashita M, Fujimoto N, Kuroda M, Kashiwa M, Sei K, Fujita M, Ike MInt J Syst Evol Microbiol10.1099/ijs.0.64667-02007Aerobiosis, Arsenates/*metabolism, Bacillus/*classification/genetics/isolation & purification/*metabolism, Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Genes, rRNA, *Industrial Waste, Japan, Molecular Sequence Data, Nitrates/metabolism, Nucleic Acid Hybridization, Oxidation-Reduction, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Selenic Acid, Selenium Compounds/*metabolism, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, *Water MicrobiologyMetabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
7442Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18226)https://www.dsmz.de/collection/catalogue/details/culture/DSM-18226
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23317J H Yoon, S S Kang, K C Lee, Y H Kho, S H Choi, K H Kang, Y H Park10.1099/00207713-51-3-1087Bacillus jeotgali sp. nov., isolated from jeotgal, Korean traditional fermented seafood.IJSEM 51: 1087-1092 200111411677
39660Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/19307
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
70906Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID88941.1StrainInfo: A central database for resolving microbial strain identifiers
120344Curators of the CIPCollection of Institut Pasteur (CIP 107104)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107104
125438Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann10.1101/2024.08.12.607695Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets
125439Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardyhttps://github.com/GenomeNet/deepGdeepG: Deep Learning for Genome Sequence Data. R package version 0.3.1