Strain identifier

BacDive ID: 1246

Type strain: Yes

Species: Salibacterium salarium

Strain Designation: BH 169

Strain history: CIP <- 2006, DSMZ <- C. J. Kim: strain BH 169 <- D. J. Park

NCBI tax ID(s): 284579 (species)

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General

@ref: 6452

BacDive-ID: 1246

DSM-Number: 16461

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, rod-shaped

description: Salibacterium salarium BH 169 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from salt lake soil.

NCBI tax id

  • NCBI tax id: 284579
  • Matching level: species

strain history

@refhistory
6452<- C.-J. Kim; BH 169 <- D.-J. Park
67771<- CJ Kim, KRIBB, BH 169 <- D.-J. Park
122358CIP <- 2006, DSMZ <- C. J. Kim: strain BH 169 <- D. J. Park

doi: 10.13145/bacdive1246.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Salibacterium
  • species: Salibacterium salarium
  • full scientific name: Salibacterium salarium (Lim et al. 2006) Gupta et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Bacillus salarius

@ref: 6452

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Salibacterium

species: Salibacterium salarium

full scientific name: Salibacterium salarium (Lim et al. 2006) Gupta et al. 2020

strain designation: BH 169

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
31491positive1.3-1.9 µm0.3-0.5 µmrod-shapedno
67771rod-shapedno
67771positive
122358positiverod-shapedyes

pigmentation

  • @ref: 31491
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6452BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514; with strain-specific modifications) Composition: NaCl 80.0 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
37479Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
122358CIP Medium 204yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=204
122358CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperaturerange
6452positivegrowth30mesophilic
31491positivegrowth15-40
31491positiveoptimum30mesophilic
37479positivegrowth30mesophilic
67771positivegrowth30mesophilic

culture pH

@refabilitytypepHPH range
31491positivegrowth6.8-9.5alkaliphile
31491positiveoptimum8

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
31491aerobe
67771aerobe
122358obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
31491NaClpositivegrowth03-20 %
31491NaClpositiveoptimum10-12 %

observation

@refobservation
31491aggregates in chains
67771quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3149122599arabinose+carbon source
3149128757fructose+carbon source
3149117234glucose+carbon source
3149117754glycerol+carbon source
3149117716lactose+carbon source
3149117306maltose+carbon source
3149129864mannitol+carbon source
3149137684mannose+carbon source
3149128053melibiose+carbon source
3149126546rhamnose+carbon source
3149117814salicin+carbon source
314914853esculin+hydrolysis
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
12235817632nitrate-reduction
12235816301nitrite-reduction

metabolite production

  • @ref: 122358
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
31491catalase+1.11.1.6
31491cytochrome oxidase+1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
122358oxidase+
122358catalase+1.11.1.6
122358urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122358-++--+---+-+-+--++--

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
122358---+/-------+/-+/-+/------------+/-------------------------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
6452salt lake soilChinaCHNAsia
67771From saline soilChinaCHNAsia
122358Environment, Soil, salt lakeChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Lake (large)
#Environmental#Terrestrial#Soil
#Condition#Saline

taxonmaps

  • @ref: 69479
  • File name: preview.99_72706.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_605;96_22576;97_28219;98_50937;99_72706&stattab=map
  • Last taxonomy: Bacillus salarius subclade
  • 16S sequence: AY667494
  • Sequence Identity:
  • Total samples: 7
  • aquatic counts: 2
  • animal counts: 5

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
64521Risk group (German classification)
1223581Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Bacillus salarius gene for 16S rRNA, partial sequence, strain: DSM 16461AB5718731530ena284579
6452Bacillus salarius strain BH169 16S ribosomal RNA gene, partial sequenceAY6674941380ena284579

Genome sequences

  • @ref: 66792
  • description: Bacillus salarius DSM 16461
  • accession: 2926589328
  • assembly level: draft
  • database: img
  • NCBI tax ID: 284579

GC content

@refGC-contentmethod
645243
6777143.0high performance liquid chromatography (HPLC)

External links

@ref: 6452

culture collection no.: DSM 16461, CIP 109218, KCTC 3912, DSM 28800

straininfo link

  • @ref: 70902
  • straininfo: 232132

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny16449443Bacillus salarius sp. nov., a halophilic, spore-forming bacterium isolated from a salt lake in China.Lim JM, Jeon CO, Lee SM, Lee JC, Xu LH, Jiang CL, Kim CJInt J Syst Evol Microbiol10.1099/ijs.0.63678-02006Bacillus/*classification/genetics/isolation & purification/*physiology, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fresh Water/*microbiology, Genes, rRNA, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/*metabolism, Spores, Bacterial/physiologyGenetics
Phylogeny19060067Bacillus aidingensis sp. nov., a moderately halophilic bacterium isolated from Ai-Ding salt lake in China.Xue Y, Ventosa A, Wang X, Ren P, Zhou P, Ma YInt J Syst Evol Microbiol10.1099/ijs.0.2008/000471-02008Bacillus/*classification/genetics/*physiology, China, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Salts, Seawater/*microbiology, Species SpecificityGenetics
Phylogeny22228653Alteribacillus bidgolensis gen. nov., sp. nov., a moderately halophilic bacterium from a hypersaline lake, and reclassification of Bacillus persepolensis as Alteribacillus persepolensis comb. nov.Didari M, Amoozegar MA, Bagheri M, Schumann P, Sproer C, Sanchez-Porro C, Ventosa AInt J Syst Evol Microbiol10.1099/ijs.0.034173-02012Bacillus/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Iran, Lakes/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/analysis, Phospholipids/analysis, *Phylogeny, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Salinity, Sequence Analysis, DNA, *Water MicrobiologyGenetics
Phylogeny26338019Salibacterium halotolerans gen. nov., sp. nov., a bacterium isolated from a salt pan, reclassification of Bacillus qingdaonensis as Salibacterium qingdaonense comb. nov. and Bacillus halochares as Salibacterium halochares comb. nov.Vishnuvardhan Reddy S, Thirumala M, Sasikala C, Venkata Ramana CInt J Syst Evol Microbiol10.1099/ijsem.0.0005722015Bacillaceae/*classification/genetics/isolation & purification, Bacillus/*classification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, India, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Sodium ChlorideTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
6452Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16461)https://www.dsmz.de/collection/catalogue/details/culture/DSM-16461
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
31491Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2877604127789
37479Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6935
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
70902Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID232132.1StrainInfo: A central database for resolving microbial strain identifiers
122358Curators of the CIPCollection of Institut Pasteur (CIP 109218)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109218