Strain identifier

BacDive ID: 1245

Type strain: Yes

Species: Fictibacillus macauensis

Strain Designation: ZFHKF-1

Strain history: CIP <- 2006, JCM, Bacillus macauensis <- 2005, H.H.P. Fang, Hong Kong Univ., Hong Kong, China: strain ZFHKF-1 <- DIT030428, Bacillus macauensis

NCBI tax ID(s): 245160 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 6862

BacDive-ID: 1245

DSM-Number: 17262

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, spore-forming, mesophilic, Gram-positive, rod-shaped

description: Fictibacillus macauensis ZFHKF-1 is a facultative anaerobe, spore-forming, mesophilic bacterium that was isolated from drinking water supply.

NCBI tax id

  • NCBI tax id: 245160
  • Matching level: species

strain history

@refhistory
6862<- H. H. P. Fang; ZFHKF-1
67770K. K. Kwon AKS293.
67770H. H. P. Fang ZFHKF-1 <-- DIT030428.
120731CIP <- 2006, JCM, Bacillus macauensis <- 2005, H.H.P. Fang, Hong Kong Univ., Hong Kong, China: strain ZFHKF-1 <- DIT030428, Bacillus macauensis

doi: 10.13145/bacdive1245.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Fictibacillus
  • species: Fictibacillus macauensis
  • full scientific name: Fictibacillus macauensis (Zhang et al. 2006) Glaeser et al. 2013

  • synonyms

    • @ref: 20215
    • synonym: Bacillus macauensis

@ref: 6862

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Fictibacillus

species: Fictibacillus macauensis

full scientific name: Fictibacillus macauensis (Zhang et al. 2006) Glaeser et al. 2013

strain designation: ZFHKF-1

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
31597positive02-05 µm0.8-1.2 µmrod-shaped
69480yes95.314
69480positive100
120731positiverod-shapedyes

colony morphology

  • @ref: 120731
  • hemolysis ability: 1

pigmentation

  • @ref: 31597
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6862CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
33279MEDIUM 368 - for Luria bertaniyesDistilled water make up to (1000.000 ml);Sodium chloride (10.000 g);Agar(15.000 g);Yeast extract (5.000 g);Tryptone (10.000 g)
120731CIP Medium 368yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=368

culture temp

@refgrowthtypetemperaturerange
6862positivegrowth30mesophilic
31597positivegrowth20-40
31597positiveoptimum30mesophilic
33279positivegrowth30mesophilic
67770positivegrowth30mesophilic

culture pH

@refabilitytypepHPH range
31597positivegrowth06-10alkaliphile
31597positiveoptimum8.5

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
31597facultative anaerobe
120731obligate aerobe

spore formation

@refspore formationconfidence
31597yes
69481yes100
69480yes100

halophily

@refsaltgrowthtested relationconcentration
31597NaClpositivegrowth2 %
31597NaClpositiveoptimum2 %

observation

@refobservation
31597aggregates in chains
67770quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3159717057cellobiose+carbon source
3159728260galactose+carbon source
3159724996lactate+carbon source
3159725115malate+carbon source
3159729864mannitol+carbon source
3159737684mannose+carbon source
3159715361pyruvate+carbon source
3159726546rhamnose+carbon source
3159733942ribose+carbon source
3159717814salicin+carbon source
3159717992sucrose+carbon source
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
683714853esculin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837115824D-fructose-builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
12073117632nitrate-reduction
12073116301nitrite-reduction

metabolite production

  • @ref: 120731
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
31597catalase+1.11.1.6
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
120731oxidase+
120731alcohol dehydrogenase-1.1.1.1
120731catalase+1.11.1.6
120731lysine decarboxylase-4.1.1.18
120731ornithine decarboxylase-4.1.1.17
120731urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120731-+++-+---+++-+-+----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
120731+/----+/------+/--+/---------------+/---------+/-------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
6862drinking water supplyMacauChinaCHNAsia
67770Influent of a drinking water treatment plant in MacauChinaCHNAsia
120731Environment, Drinking water, supply of MacaoPearl riverChinaCHNAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Freshwater

taxonmaps

  • @ref: 69479
  • File name: preview.99_5876.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_1870;97_3456;98_4369;99_5876&stattab=map
  • Last taxonomy: Fictibacillus macauensis subclade
  • 16S sequence: AY373018
  • Sequence Identity:
  • Total samples: 336
  • soil counts: 158
  • aquatic counts: 27
  • animal counts: 141
  • plant counts: 10

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
68621Risk group (German classification)
1207311Risk group (French classification)

Sequence information

16S sequences

  • @ref: 6862
  • description: Bacillus macauensis strain ZFHKF-1 16S ribosomal RNA gene, partial sequence
  • accession: AY373018
  • length: 1355
  • database: ena
  • NCBI tax ID: 1196324

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Bacillus macauensis ZFHKF-11196324.3wgspatric1196324
66792Fictibacillus macauensis ZFHKF-12524023182draftimg1196324
67770Fictibacillus macauensis ZFHKF-1GCA_000269865contigncbi1196324

GC content

@refGC-contentmethod
686240.8
6777040.6genome sequence analysis
6777040.8high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileyes82.747no
gram-positiveyes92.113yes
anaerobicno98.234yes
aerobicyes85.709yes
halophileno87.691no
spore-formingyes95.564no
glucose-utilyes89.688no
flagellatedyes65.995no
thermophileno92.758yes
glucose-fermentno91.227no

External links

@ref: 6862

culture collection no.: DSM 17262, JCM 13285, CIP 109160, NCIMB 14300

straininfo link

  • @ref: 70901
  • straininfo: 289967

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny16449438Bacillus macauensis sp. nov., a long-chain bacterium isolated from a drinking water supply.Zhang T, Fan X, Hanada S, Kamagata Y, Fang HHPInt J Syst Evol Microbiol10.1099/ijs.0.63868-02006Anaerobiosis, Bacillus/*classification/genetics/isolation & purification/*physiology, Bacterial Typing Techniques, China, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fresh Water/*microbiology, Genes, rRNA, Genotype, Microscopy, Confocal, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Water SupplyGenetics
Phylogeny19459975Bacillus tianmuensis sp. nov., isolated from soil in Tianmu Mountain national natural reserve, Hangzhou, China.Wen YP, Wu XC, Qian CD, Zhao YH, Fang HH, Li OFEMS Microbiol Lett10.1111/j.1574-6968.2009.01610.x2009Bacillus/chemistry/*classification/genetics/*isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, China, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil MicrobiologyGenetics
Phylogeny19502335Bacillus solisalsi sp. nov., a halotolerant, alkaliphilic bacterium isolated from soil around a salt lake.Liu H, Zhou Y, Liu R, Zhang KY, Lai RInt J Syst Evol Microbiol10.1099/ijs.0.000653-02009Bacillus/*classification/genetics/isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fatty Acids/analysis, Fresh Water/*microbiology, Hydrogen-Ion Concentration, Lipids/analysis, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, Polymerase Chain Reaction/methods, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Sodium Chloride/metabolism, *Soil Microbiology, Species SpecificityGenetics
Genetics22887677Genome of Bacillus macauensis ZFHKF-1, a long-chain-forming bacterium.Cai L, Zhang TJ Bacteriol10.1128/JB.01049-122012Bacillus/classification/*genetics/growth & development/isolation & purification, Base Sequence, Chromosome Mapping, *Genome, Bacterial, Molecular Sequence Data, Sequence Analysis, DNAPhylogeny
Phylogeny23355698Fictibacillus phosphorivorans gen. nov., sp. nov. and proposal to reclassify Bacillus arsenicus, Bacillus barbaricus, Bacillus macauensis, Bacillus nanhaiensis, Bacillus rigui, Bacillus solisalsi and Bacillus gelatini in the genus Fictibacillus.Glaeser SP, Dott W, Busse HJ, Kampfer PInt J Syst Evol Microbiol10.1099/ijs.0.049171-02013Bacillus/*classification/genetics/isolation & purification, Bacterial Typing Techniques, DNA, Bacterial/genetics, Fatty Acids/analysis, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/analysis, Phospholipids/analysis, *Phylogeny, Polyamines/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/analysisGenetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
6862Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17262)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17262
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
31597Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2788528776041
33279Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6870
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
70901Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID289967.1StrainInfo: A central database for resolving microbial strain identifiers
120731Curators of the CIPCollection of Institut Pasteur (CIP 109160)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109160