Strain identifier

BacDive ID: 1242

Type strain: Yes

Species: Evansella cellulosilytica

Strain Designation: N-4

Strain history: CIP <- 2005, JCM <- 1994, K. Horikoshi, Japan Ag. Marine-Earth Science Technol., Yokosuka, Japan: strain N-4

NCBI tax ID(s): 649639 (strain), 1413 (species)

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General

@ref: 1110

BacDive-ID: 1242

DSM-Number: 2522

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped, colony-forming

description: Evansella cellulosilytica N-4 is an aerobe, spore-forming, mesophilic bacterium that forms irregular colonies and builds sporangium.

NCBI tax id

NCBI tax idMatching level
649639strain
1413species

strain history

@refhistory
1110<- ATCC <- Rikagaku Kenkyusho, N-4
67770K. Horikoshi N-4.
117240CIP <- 2005, JCM <- 1994, K. Horikoshi, Japan Ag. Marine-Earth Science Technol., Yokosuka, Japan: strain N-4

doi: 10.13145/bacdive1242.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Evansella
  • species: Evansella cellulosilytica
  • full scientific name: Evansella cellulosilytica corrig. (Nogi et al. 2005) Gupta et al. 2020

  • synonyms

    @refsynonym
    20215Evansella cellulosilyticus
    20215Bacillus cellulosilyticus

@ref: 1110

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Evansella

species: Evansella cellulosilytica

full scientific name: Evansella cellulosilytica (Nogi et al. 2005) Gupta et al. 2020

strain designation: N-4

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityflagellum arrangementconfidence
31483positive2.5 µm0.35 µmrod-shapedyes
27781positive2-3 µm0.3-0.4 µmrod-shapedyesperitrichous
69480yes93.214
69480positive100
117240positiverod-shapedno

colony morphology

@refcolony colorcolony shape
27781cream-colouredirregular
117240

multicellular morphology

  • @ref: 27781
  • forms multicellular complex: yes
  • complex name: sporangium
  • further description: swollen

pigmentation

  • @ref: 31483
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
1110ALKALINE NUTRIENT AGAR (DSMZ Medium 31)yeshttps://mediadive.dsmz.de/medium/31Name: ALKALINE NUTRIENT AGAR (DSMZ Medium 31) Composition: Agar 15.0 g/l Na2CO3 anhydrous 5.3 g/l Peptone 5.0 g/l NaHCO3 4.2 g/l Meat extract 3.0 g/l Distilled water
34981MEDIUM 291 - for Bacillus hortiyesDistilled water make up to (900.000 ml);Magnesium sulphate heptahydrate (0.200 g);Agar (15.000 g);Glucose (10.000 g);Yeast extract (5.000 g);Potassium di-hydrogen phosphate (1.000 g);Polypeptone (5.000 g);Sodium carbonate solution - M00442 (100.000 ml)
117240CIP Medium 291yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=291

culture temp

@refgrowthtypetemperaturerange
1110positivegrowth30mesophilic
31483positivegrowth20-40
31483positiveoptimum37mesophilic
34981positivegrowth37mesophilic
27781positiveoptimum37mesophilic
27781positivegrowth20-40
67770positivegrowth37mesophilic

culture pH

@refabilitytypepHPH range
31483positivegrowth08-10alkaliphile
31483positiveoptimum9.5
27781positivegrowth8-10
27781positiveoptimum9-10

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
31483aerobe
117240obligate aerobe

spore formation

@refspore formationspore descriptiontype of sporeconfidence
31483yes
27781yessubtermiminalspore
69481yes100
69480yes100

compound production

  • @ref: 1110
  • compound: cellulase, alkaline

halophily

@refsaltgrowthtested relationconcentration
31483NaClpositivegrowth0-13 %
31483NaClpositiveoptimum7.5 %
27781NaClpositivegrowth12 %(w/v)

observation

@refobservation
31483aggregates in clumps
67770quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3148353425tween 60+carbon source
3148353423tween 40+carbon source
3148353424tween 20+carbon source
3148327082trehalose+carbon source
3148317992sucrose+carbon source
3148317814salicin+carbon source
3148337684mannose+carbon source
3148317306maltose+carbon source
3148317716lactose+carbon source
3148317234glucose+carbon source
3148328757fructose+carbon source
3148317057cellobiose+carbon source
3148317632nitrate+reduction
2778118305arbutin-builds gas from
2778118305arbutin+builds acid from
27781casein-
2778117057cellobiose-builds gas from
2778117057cellobiose+builds acid from
2778162968cellulose+hydrolysis
2778115824D-fructose-builds gas from
2778115824D-fructose+builds acid from
2778117634D-glucose-builds gas from
2778117634D-glucose+builds acid from
2778117716lactose-builds gas from
2778117716lactose+builds acid from
2778117306maltose-builds gas from
2778117306maltose+builds acid from
2778116024D-mannose-builds gas from
2778116024D-mannose+builds acid from
2778116551D-trehalose-builds gas from
2778116551D-trehalose+builds acid from
277815291gelatin-hydrolysis
2778161993maltotriose-builds gas from
2778161993maltotriose+builds acid from
2778117632nitrate+reduction
2778117814salicin-builds gas from
2778117814salicin+builds acid from
2778128017starch+hydrolysis
2778117992sucrose-builds gas from
2778117992sucrose+builds acid from
2778132528turanose-builds gas from
2778132528turanose+builds acid from
2778153424tween 20+hydrolysis
2778153423tween 40+hydrolysis
2778153425tween 60+hydrolysis
68371Potassium 5-ketogluconate+builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837128847D-fucose-builds acid from
6837162318D-lyxose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
683714853esculin+builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837115824D-fructose-builds acid from
6837117634D-glucose-builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
11724017632nitrate+reduction
11724016301nitrite-reduction

metabolite production

@refChebi-IDmetaboliteproduction
2778116136hydrogen sulfideno
2778135581indoleno
11724035581indoleno

enzymes

@refvalueactivityec
31483catalase+1.11.1.6
27781catalase+1.11.1.6
27781cytochrome oxidase+1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
67770cellulase3.2.1.4
117240oxidase-
117240alcohol dehydrogenase-1.1.1.1
117240catalase+1.11.1.6
117240lysine decarboxylase-4.1.1.18
117240ornithine decarboxylase-4.1.1.17
117240urease+3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
117240-+++-+---++-++-++---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
117240------------+/------------+--------------+/--+/--+/-----+

Isolation, sampling and environmental information

isolation

@refsample type
1110soil
67770Soil
117240Environment, Soil

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_5602.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_2701;97_3317;98_4179;99_5602&stattab=map
  • Last taxonomy: Bacillus cellulosilyticus
  • 16S sequence: AB043852
  • Sequence Identity:
  • Total samples: 543
  • soil counts: 173
  • aquatic counts: 105
  • animal counts: 235
  • plant counts: 30

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
11101Risk group (German classification)
1172401Risk group (French classification)

Sequence information

16S sequences

  • @ref: 31483
  • description: Bacillus cellulosilyticus gene for 16S rRNA
  • accession: AB043852
  • length: 1505
  • database: nuccore
  • NCBI tax ID: 649639

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Evansella cellulosilytica DSM 2522GCA_000177235completencbi649639
66792Bacillus cellulosilyticus DSM 2522649639.5completepatric649639
66792Bacillus cellulosilyticus N-4, DSM 2522649633010completeimg649639

GC content

@refGC-contentmethod
111036.1
3148339.6
2778139.6high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileyes91.3yes
flagellatedyes90.007yes
gram-positiveyes88.993yes
anaerobicno97.294yes
aerobicyes69.89no
halophileyes81.524yes
spore-formingyes95.453no
thermophileno96.847yes
glucose-utilyes88.047yes
glucose-fermentno90.856no

External links

@ref: 1110

culture collection no.: DSM 2522, ATCC 21833, FERM P-1141, JCM 9156, CIP 109017, FERM 1141

straininfo link

  • @ref: 70898
  • straininfo: 37749

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny16280488Characterization of alkaliphilic Bacillus strains used in industry: proposal of five novel species.Nogi Y, Takami H, Horikoshi KInt J Syst Evol Microbiol10.1099/ijs.0.63649-02005Bacillus/*classification/genetics/isolation & purification, DNA, Bacterial/analysis/genetics, DNA, Ribosomal/genetics, Fatty Acids/analysis, *Industrial Microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/*analysis/geneticsGenetics
Phylogeny24756808Bacillus alkalicola sp. nov., an alkaliphilic, gram-positive bacterium isolated from Zhabuye Lake in Tibet, China.Zhai L, Ma Y, Xue Y, Ma YCurr Microbiol10.1007/s00284-014-0576-x2014Bacillus/*classification/genetics/*isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, Cluster Analysis, Cytosol/chemistry, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Hydrogen-Ion Concentration, Lakes/*microbiology, Molecular Sequence Data, Peptidoglycan/analysis, Phospholipids/analysis, Phylogeny, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/metabolism, Spores, Bacterial/cytology, Temperature, TibetGenetics
Phylogeny25908708Bacillus caseinilyticus sp. nov., an alkali- and thermotolerant bacterium isolated from a soda lake.Vishnuvardhan Reddy S, Thirumala M, Farooq MInt J Syst Evol Microbiol10.1099/ijs.0.0002752015Alkalies/chemistry, Bacillus/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, India, Lakes/microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry, *Water MicrobiologyGenetics
Phylogeny29297846Bacillus tamaricis sp. nov., an alkaliphilic bacterium isolated from a Tamarix cone soil.Zhang YG, Zhou XK, Guo JW, Xiao M, Wang HF, Wang Y, Bobodzhanova K, Li WJInt J Syst Evol Microbiol10.1099/ijsem.0.0025432018Bacillus/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Phospholipids/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Tamaricaceae/*microbiology, Vitamin K 2/analogs & derivatives/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitlepubmedID_cross_referencejournal
1110Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2522)https://www.dsmz.de/collection/catalogue/details/culture/DSM-2522
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
2778110.1099/ijs.0.63649-016280488
31483Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2877604127781
34981Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6712
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updates33211880Nucleic Acids Res. 49: D498-D508 2020
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
70898Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID37749.1StrainInfo: A central database for resolving microbial strain identifiers
117240Curators of the CIPCollection of Institut Pasteur (CIP 109017)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109017