Strain identifier

BacDive ID: 1226

Type strain: Yes

Species: Pseudalkalibacillus hwajinpoensis

Strain Designation: SW-72

Strain history: CIP <- 2004, JCM <- 2002, J.H. Yoon: strain SW-72

NCBI tax ID(s): 208199 (species)

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General

@ref: 6325

BacDive-ID: 1226

DSM-Number: 16206

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-variable, rod-shaped

description: Pseudalkalibacillus hwajinpoensis SW-72 is an aerobe, spore-forming, mesophilic bacterium that was isolated from sea water.

NCBI tax id

  • NCBI tax id: 208199
  • Matching level: species

strain history

@refhistory
6325<- F. G. Priest, Univ. Edinburgh <- J.-H. Yoon; SW-72
67770J.-H. Yoon SW-72.
119993CIP <- 2004, JCM <- 2002, J.H. Yoon: strain SW-72

doi: 10.13145/bacdive1226.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Pseudalkalibacillus
  • species: Pseudalkalibacillus hwajinpoensis
  • full scientific name: Pseudalkalibacillus hwajinpoensis (Yoon et al. 2004) Joshi et al. 2022

  • synonyms

    @refsynonym
    20215Alkalihalobacillus hwajinpoensis
    20215Bacillus hwajinpoensis

@ref: 6325

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Caryophanales

family: Bacillaceae

genus: Pseudalkalibacillus

species: Pseudalkalibacillus hwajinpoensis

full scientific name: Pseudalkalibacillus hwajinpoensis (Yoon et al. 2004) Joshi et al. 2022

strain designation: SW-72

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
29923variable3.25 µm1.15 µmrod-shapedno
119993positiverod-shapedyes

colony morphology

  • @ref: 119993

pigmentation

  • @ref: 29923
  • production: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6325BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
40002Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
119993CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperaturerange
6325positivegrowth30mesophilic
29923positivegrowth10-40
29923positiveoptimum32.5mesophilic
40002positivegrowth30mesophilic
67770positivegrowth30mesophilic
119993positivegrowth10-37
119993nogrowth45thermophilic
119993nogrowth55thermophilic

culture pH

@refabilitytypepH
29923positivegrowth05-07
29923positiveoptimum6.5
119993positivegrowth6

Physiology and metabolism

oxygen tolerance

  • @ref: 29923
  • oxygen tolerance: aerobe

spore formation

  • @ref: 29923
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
29923NaClpositivegrowth0.1-19 %
29923NaClpositiveoptimum3.5 %
119993NaClpositivegrowth2-4 %
119993NaClnogrowth0 %
119993NaClnogrowth6 %
119993NaClnogrowth8 %
119993NaClnogrowth10 %

observation

  • @ref: 67770
  • observation: quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2992317057cellobiose+carbon source
2992328260galactose+carbon source
299235291gelatin+carbon source
2992329864mannitol+carbon source
2992337684mannose+carbon source
2992328053melibiose+carbon source
2992316634raffinose+carbon source
2992333942ribose+carbon source
2992353424tween 20+carbon source
2992353423tween 40+carbon source
2992353425tween 60+carbon source
2992353426tween 80+carbon source
299234853esculin+hydrolysis
2992317632nitrate+reduction
68371Potassium 2-ketogluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen+builds acid from
6837128017starch+builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose+builds acid from
6837128053melibiose+builds acid from
6837117716lactose-builds acid from
6837117306maltose+builds acid from
683714853esculin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine+builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol+builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose+builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
11999316947citrate-carbon source
1199934853esculin+hydrolysis
119993606565hippurate+hydrolysis
11999317632nitrate+reduction
11999316301nitrite-reduction
11999317632nitrate-respiration

metabolite production

  • @ref: 119993
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11999315688acetoin-
11999317234glucose-

enzymes

@refvalueactivityec
29923catalase+1.11.1.6
29923gelatinase+
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
119993oxidase-
119993beta-galactosidase+3.2.1.23
119993alcohol dehydrogenase-1.1.1.1
119993gelatinase+
119993amylase+
119993DNase+
119993catalase+1.11.1.6
119993tween esterase+
119993gamma-glutamyltransferase-2.3.2.2
119993lecithinase+
119993lysine decarboxylase-4.1.1.18
119993ornithine decarboxylase-4.1.1.17
119993protease+
119993urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119993-+++-----+++-+-+----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
119993+/----+/-----++++----+---+---+/-+/-+-++----++---------+/--+/-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
6325sea waterEast Sea, Hwajinpo BeachRepublic of KoreaKORAsia
67770SeawaterHwajinpo BeachRepublic of KoreaKORAsia
119993Environment, Sea waterHwajinpo beachRepublic of KoreaKORAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Marine

taxonmaps

  • @ref: 69479
  • File name: preview.99_6943.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_3264;97_4003;98_5107;99_6943&stattab=map
  • Last taxonomy: Alkalihalobacillus
  • 16S sequence: AF541966
  • Sequence Identity:
  • Total samples: 1394
  • soil counts: 316
  • aquatic counts: 683
  • animal counts: 370
  • plant counts: 25

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
63251Risk group (German classification)
1199931Risk group (French classification)

Sequence information

16S sequences

  • @ref: 6325
  • description: Bacillus hwajinpoensis 16S ribosomal RNA gene, partial sequence
  • accession: AF541966
  • length: 1507
  • database: ena
  • NCBI tax ID: 208199

Genome sequences

  • @ref: 66792
  • description: Pseudalkalibacillus hwajinpoensis DSM 16206
  • accession: GCA_029277305
  • assembly level: complete
  • database: ncbi
  • NCBI tax ID: 208199

GC content

@refGC-contentmethod
632540.9high performance liquid chromatography (HPLC)
2992340.9

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno55.208yes
gram-positiveyes81.411yes
anaerobicno98.282yes
aerobicyes92.664yes
halophileyes82.563no
spore-formingyes92.166yes
glucose-fermentno93.108no
thermophileno97.926no
glucose-utilyes91.486no
motileyes79.818no

External links

@ref: 6325

culture collection no.: DSM 16206, JCM 11807, KCCM 41641, CIP 108441, LMG 24749

straininfo link

  • @ref: 70882
  • straininfo: 132012

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny15143027Bacillus hwajinpoensis sp. nov. and an unnamed Bacillus genomospecies, novel members of Bacillus rRNA group 6 isolated from sea water of the East Sea and the Yellow Sea in Korea.Yoon JH, Kim IG, Kang KH, Oh TK, Park YHInt J Syst Evol Microbiol10.1099/ijs.0.02678-02004Bacillus/*classification/genetics/*isolation & purification/metabolism, Base Composition, Cell Wall/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Fatty Acids/analysis, Korea, Molecular Sequence Data, Peptidoglycan/chemistry, Phenotype, Phylogeny, RNA, Bacterial/*genetics, RNA, Ribosomal, 16S/*genetics, Seawater/microbiologyGenetics
Phylogeny21278416Bacillus hemicentroti sp. nov., a moderate halophile isolated from a sea urchin.Chen YG, Zhang YQ, He JW, Klenk HP, Xiao JQ, Zhu HY, Tang SK, Li WJInt J Syst Evol Microbiol10.1099/ijs.0.026732-02011Animals, Bacillus/*classification/genetics/*isolation & purification/metabolism, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sea Urchins/*microbiologyMetabolism
Phylogeny30600415Lottiidibacillus patelloidae gen. nov., sp. nov., isolated from the intestinal tract of a marine limpet and reclassification of Bacillus taeanensis as Maribacillus taeanensis gen. nov., comb. nov.Liu R, Huang Z, Dong C, Shao ZAntonie Van Leeuwenhoek10.1007/s10482-018-01213-z2019Animals, Bacillaceae/*classification/genetics/isolation & purification/metabolism, Bacillus/*classification/genetics/isolation & purification/metabolism, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry/metabolism, Gastropoda/*microbiology, Intestines/*microbiology, Phylogeny, RNA, Ribosomal, 16S/geneticsMetabolism
Phylogeny32100673Bacillus caeni sp. nov., isolated from mangrove sediment.Mo K, Huang H, Bao S, Hu YInt J Syst Evol Microbiol10.1099/ijsem.0.0038532020Bacillus/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
6325Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16206)https://www.dsmz.de/collection/catalogue/details/culture/DSM-16206
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29923Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2629428776041
40002Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6064
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
70882Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID132012.1StrainInfo: A central database for resolving microbial strain identifiers
119993Curators of the CIPCollection of Institut Pasteur (CIP 108441)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108441