Strain identifier

BacDive ID: 1224

Type strain: Yes

Species: Rossellomorea marisflavi

Strain Designation: TF-11

Strain history: CIP <- 2008, JCM <- 2002, Y.H. Park: strain TF-11

NCBI tax ID(s): 189381 (species)

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General

@ref: 6323

BacDive-ID: 1224

DSM-Number: 16204

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped, colony-forming

description: Rossellomorea marisflavi TF-11 is an aerobe, spore-forming, mesophilic bacterium that forms circular colonies and was isolated from sea water of a tidal flat.

NCBI tax id

  • NCBI tax id: 189381
  • Matching level: species

strain history

@refhistory
6323<- F. G. Priest, Univ. Edinburgh <- J.-H. Yoon; TF-11
67770Y.-H. Park TF-11.
120830CIP <- 2008, JCM <- 2002, Y.H. Park: strain TF-11

doi: 10.13145/bacdive1224.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Rossellomorea
  • species: Rossellomorea marisflavi
  • full scientific name: Rossellomorea marisflavi (Yoon et al. 2003) Gupta et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Bacillus marisflavi

@ref: 6323

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Rossellomorea

species: Rossellomorea marisflavi

full scientific name: Rossellomorea marisflavi (Yoon et al. 2003) Gupta et al. 2020

strain designation: TF-11

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityflagellum arrangement
23314positive1.5-3.5 µm0.6-0.8 µmrod-shapedyesmonotrichous, polar
120830positiverod-shapedyes

colony morphology

@refcolony sizecolony colorcolony shapeincubation periodmedium used
233142-4 mmpale yellowcircular3-4 daysMA
120830

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6323BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
32891Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
120830CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13
120830CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
6323positivegrowth30mesophilic
23314nogrowth>48thermophilic
23314positivegrowth10-47
23314nogrowth4psychrophilic
23314positiveoptimum30-37mesophilic
32891positivegrowth30mesophilic
67770positivegrowth30mesophilic
120830positivegrowth22-45
120830nogrowth10psychrophilic
120830nogrowth55thermophilic

culture pH

@refabilitytypepH
23314positiveoptimum6.0-8.0
23314positivegrowth4.5
23314nogrowth4.0
120830positivegrowth6

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
23314aerobe
120830obligate aerobe

spore formation

  • @ref: 23314
  • spore description: ellipsoidal, central or subterminal, swollen sporangia
  • type of spore: endospore
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentrationhalophily level
23314NaClpositiveoptimum2-5 %
23314NaClpositivegrowth0-16 %
23314moderately halophilic
120830NaClpositivegrowth0-6 %
120830NaClnogrowth8 %
120830NaClnogrowth10 %

observation

  • @ref: 67770
  • observation: quinones: MK-7

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371lactose-builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside+builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
120830citrate-carbon source16947
120830esculin-hydrolysis4853
120830hippurate+hydrolysis606565
120830nitrate-reduction17632
120830nitrite-reduction16301
120830nitrate+respiration17632

metabolite production

  • @ref: 120830
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12083015688acetoin-
12083017234glucose+

enzymes

@refvalueactivityec
23314catalase+1.11.1.6
23314cytochrome oxidase-1.9.3.1
23314urease-3.5.1.5
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
120830oxidase+
120830beta-galactosidase+3.2.1.23
120830alcohol dehydrogenase-1.1.1.1
120830gelatinase-
120830amylase-
120830DNase-
120830caseinase-3.4.21.50
120830catalase+1.11.1.6
120830tween esterase-
120830gamma-glutamyltransferase+2.3.2.2
120830lecithinase-
120830lipase-
120830lysine decarboxylase+4.1.1.18
120830ornithine decarboxylase-4.1.1.17
120830urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120830-+++-+---+++-+-+----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
120830+/----+-----+++----+-+-+-+++++-+++--+/-+/---+/-----------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120830+++++-+--++++++--+-+++-++------+-+--+-+----++--+++----++-+-+++------++--++-++--+++++--+--++++-+-+-+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
6323sea water of a tidal flatYellow SeaRepublic of KoreaKORAsia
67770Seawater of a tidal flat of the Yellow SeaRepublic of KoreaKORAsia
120830Environment, Sea water of a tidal flat of the Yellow SeaYellow SeaRepublic of KoreaKORAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Environmental#Terrestrial#Tidal flat

taxonmaps

  • @ref: 69479
  • File name: preview.99_3908.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_1652;97_1976;98_2430;99_3908&stattab=map
  • Last taxonomy: Bacillus marisflavi
  • 16S sequence: AF483624
  • Sequence Identity:
  • Total samples: 200
  • soil counts: 50
  • aquatic counts: 39
  • animal counts: 91
  • plant counts: 20

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
63231Risk group (German classification)
1208301Risk group (French classification)

Sequence information

16S sequences

  • @ref: 6323
  • description: Bacillus marisflavi strain TF-11 16S ribosomal RNA gene, partial sequence
  • accession: AF483624
  • length: 1506
  • database: ena
  • NCBI tax ID: 189381

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Bacillus marisflavi strain JCM 11544189381.12wgspatric189381
66792Rossellomorea marisflavi JCM 115442645727550draftimg189381
67770Rossellomorea marisflavi JCM 11544GCA_001274775scaffoldncbi189381

GC content

@refGC-contentmethod
632349high performance liquid chromatography (HPLC)
2331449.00high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes90.419yes
gram-positiveyes89.493no
anaerobicno97.843yes
aerobicyes92.019yes
halophileyes64.434no
spore-formingyes95.844yes
glucose-utilyes91.141no
flagellatedyes91.856yes
thermophileno98.599no
glucose-fermentno89.176no

External links

@ref: 6323

culture collection no.: DSM 16204, JCM 11544, KCCM 41588, CIP 108135

straininfo link

  • @ref: 70880
  • straininfo: 127981

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny13130010Bacillus marisflavi sp. nov. and Bacillus aquimaris sp. nov., isolated from sea water of a tidal flat of the Yellow Sea in Korea.Yoon JH, Kim IG, Kang KH, Oh TK, Park YHInt J Syst Evol Microbiol10.1099/ijs.0.02365-02003Bacillus/classification/genetics/*isolation & purification/metabolism, Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Fatty Acids/analysis, Korea, Microscopy, Electron, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Seawater/microbiology, Species SpecificityGenetics
Phylogeny16902027Bacillus seohaeanensis sp. nov., a halotolerant bacterium that contains L-lysine in its cell wall.Lee JC, Lim JM, Park DJ, Jeon CO, Li WJ, Kim CJInt J Syst Evol Microbiol10.1099/ijs.0.64237-02006Bacillus/chemistry/*classification/isolation & purification/physiology, Base Composition, Cell Wall/chemistry, Culture Media, Cyanoacrylates/analysis, DNA, Bacterial/genetics, Fatty Acids/analysis, *Industrial Microbiology, Lysine/analysis/metabolism, Molecular Sequence Data, Peptidoglycan/analysis/metabolism, Phenotype, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Homology, Nucleic Acid, Sodium Chloride, Species Specificity, Temperature, Vitamin K 2/analogs & derivativesCultivation
Phylogeny24860111Bacillus oryzaecorticis sp. nov., a moderately halophilic bacterium isolated from rice husks.Hong SW, Kwon SW, Kim SJ, Kim SY, Kim JJ, Lee JS, Oh MH, Kim AJ, Chung KSInt J Syst Evol Microbiol10.1099/ijs.0.058768-02014Bacillus/*classification/genetics/isolation & purification, Bacterial Typing Techniques, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Oryza/*microbiology, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, Vitamin K 2/chemistryGenetics
Phylogeny25100188Bacillus haikouensis sp. nov., a facultatively anaerobic halotolerant bacterium isolated from a paddy soil.Li J, Yang G, Lu Q, Zhao Y, Zhou SAntonie Van Leeuwenhoek10.1007/s10482-014-0248-72014Aerobiosis, Anaerobiosis, Bacillus/*classification/genetics/*isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, China, Cluster Analysis, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Hydrogen-Ion Concentration, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/analysis, Phospholipids/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/metabolism, *Soil Microbiology, Spores, Bacterial/cytology, Temperature, Vitamin K 2/analysisGenetics
Genetics26659687Draft Genome Sequence of Bacillus marisflavi TF-11T (JCM 11544), a Carotenoid-Producing Bacterium Isolated from Seawater from a Tidal Flat in the Yellow Sea.Wang JP, Liu B, Liu GH, Chen DJ, Chen QQ, Zhu YJ, Chen Z, Che JMGenome Announc10.1128/genomeA.01451-152015Phylogeny
Phylogeny30767851Bacillus salacetis sp. nov., a slightly halophilic bacterium from Thai shrimp paste (Ka-pi).Daroonpunt R, Yiamsombut S, Sitdhipol J, Tanasupawat SInt J Syst Evol Microbiol10.1099/ijsem.0.0032862019Bacillus/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, *Food Microbiology, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seafood/*microbiology, Sequence Analysis, DNA, Thailand, Vitamin K 2/analogs & derivatives/chemistryBiotechnology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
6323Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16204)https://www.dsmz.de/collection/catalogue/details/culture/DSM-16204
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23314Jung-Hoon Yoon, In-Gi Kim, Kook Hee Kang, Tae-Kwang Oh, Yong-Ha Park10.1099/ijs.0.02365-0Bacillus marisflavi sp. nov. and Bacillus aquimaris sp. nov., isolated from sea water of a tidal flat of the Yellow Sea in KoreaIJSEM 53: 1297-1303 200313130010
32891Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5724
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
70880Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID127981.1StrainInfo: A central database for resolving microbial strain identifiers
120830Curators of the CIPCollection of Institut Pasteur (CIP 108135)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108135