Strain identifier
BacDive ID: 12217
Type strain:
Species: Sorangium bulgaricum
Strain Designation: So ce321
Strain history: <- H. Reichenbach, GBF; So ce321
NCBI tax ID(s): 2093423 (species)
General
@ref: 15008
BacDive-ID: 12217
DSM-Number: 53339
keywords: Bacteria, spore-forming, mesophilic, Gram-negative, rod-shaped
description: Sorangium bulgaricum So ce321 is a spore-forming, mesophilic, Gram-negative bacterium that builds a fruiting body and was isolated from soil with plant residues.
NCBI tax id
- NCBI tax id: 2093423
- Matching level: species
strain history
- @ref: 15008
- history: <- H. Reichenbach, GBF; So ce321
doi: 10.13145/bacdive12217.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Deltaproteobacteria
- order: Myxococcales
- family: Polyangiaceae
- genus: Sorangium
- species: Sorangium bulgaricum
- full scientific name: Sorangium bulgaricum Mohr et al. 2018
@ref: 15008
domain: Bacteria
phylum: Proteobacteria
class: Deltaproteobacteria
order: Myxococcales
family: Polyangiaceae
genus: Sorangium
species: Sorangium bulgaricum
full scientific name: Sorangium bulgaricum Mohr et al. 2018
strain designation: So ce321
type strain: yes
Morphology
cell morphology
- @ref: 66744
- gram stain: negative
- cell length: 4.0-7.0 µm
- cell width: 0.7-1.2 µm
- cell shape: rod-shaped
- motility: no
colony morphology
@ref | colony color | medium used | colony shape |
---|---|---|---|
66744 | bright orange | P agar | |
66744 | bright orange | CY-AGAR | |
66744 | bright orange | liquid medium | |
66744 | orange-transparent | VY/2 AGAR | circular |
multicellular morphology
- @ref: 18281
- forms multicellular complex: yes
- complex name: fruiting body
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
15008 | VY/2 AGAR (DSMZ Medium 9) | yes | https://mediadive.dsmz.de/medium/9 | Name: VY/2 AGAR (DSMZ Medium 9) Composition: Agar 15.0 g/l Baker's yeast 5.0 g/l CaCl2 x 2 H2O 1.36 g/l Vitamin B12 0.0005 g/l Distilled water |
66744 | CY-AGAR | yes | ||
66744 | liquid medium | yes | ||
66744 | P agar | yes | ||
66744 | VY/2 AGAR | yes |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
15008 | positive | growth | 28 | mesophilic |
66744 | positive | optimum | 30 | mesophilic |
66744 | positive | growth | 18-37 |
culture pH
@ref | ability | type | pH |
---|---|---|---|
66744 | positive | optimum | 7.5 |
66744 | positive | growth | 6.5-8.0 |
Physiology and metabolism
spore formation
- @ref: 66744
- spore description: 0.7 µm wide and 0.95 µm long
- type of spore: myxospore
- spore formation: yes
observation
- @ref: 66744
- observation: On CY agar, the strain grows in veins. On VY/2 and Stan21 with filter paper, corrosion is extensive after several weeks. Black sporangioles can be observed on Stan 21 with filter paper after several weeks.
antibiotic resistance
@ref | ChEBI | metabolite | is antibiotic | is sensitive | sensitivity conc. | is resistant | resistance conc. |
---|---|---|---|---|---|---|---|
66744 | 23066 | cephalosporin | yes | yes | 50 µg/mL | ||
66744 | 71321 | fusidate | yes | yes | 50 µg/mL | ||
66744 | 24753 | hygromycin | yes | yes | 50 µg/mL | ||
66744 | 27701 | oxytetracycline | yes | yes | 50 µg/mL | ||
66744 | 9215 | spectinomycin | yes | yes | 50 µg/mL | ||
66744 | 29693 | thiostrepton | yes | yes | 50 µg/mL | ||
66744 | 28971 | ampicillin | yes | yes | 50 µg/mL | ||
66744 | 28669 | bacitracin | yes | yes | 50 µg/mL | ||
66744 | 17833 | gentamicin | yes | yes | 50 µg/mL | ||
66744 | 6104 | kanamycin | yes | yes | 50 µg/mL | ||
66744 | 59062 | polymyxin | yes | yes | 50 µg/mL | ||
66744 | 45924 | trimethoprim | yes | yes | 50 µg/mL |
enzymes
@ref | value | activity | ec |
---|---|---|---|
66744 | alpha-fucosidase | - | 3.2.1.51 |
66744 | alpha-glucosidase | - | 3.2.1.20 |
66744 | alpha-mannosidase | - | 3.2.1.24 |
66744 | beta-galactosidase | - | 3.2.1.23 |
66744 | beta-glucuronidase | - | 3.2.1.31 |
66744 | acid phosphatase | + | 3.1.3.2 |
66744 | alkaline phosphatase | + | 3.1.3.1 |
66744 | alpha-chymotrypsin | + | 3.4.21.1 |
66744 | alpha-galactosidase | + | 3.2.1.22 |
66744 | beta-glucosidase | + | 3.2.1.21 |
66744 | catalase | + | 1.11.1.6 |
66744 | cystine arylamidase | + | 3.4.11.3 |
66744 | cytochrome oxidase | + | 1.9.3.1 |
66744 | esterase (C 4) | + | |
66744 | esterase Lipase (C 8) | + | |
66744 | leucine aminopeptidase | + | 3.4.1.1 |
66744 | leucine arylamidase | + | 3.4.11.1 |
66744 | lipase (C 14) | + | |
66744 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
66744 | naphthol-AS-BI-phosphohydrolase | + | |
66744 | trypsin | + | 3.4.21.4 |
66744 | valine arylamidase | + |
Isolation, sampling and environmental information
isolation
@ref | sample type | country | origin.country | continent |
---|---|---|---|---|
15008 | soil with plant residues | Bulgaria | BGR | Europe |
66744 | soil | Bulgaria | BGR | Europe |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Terrestrial | #Soil |
#Host | #Plants |
Safety information
risk assessment
- @ref: 15008
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
GC content
- @ref: 66744
- GC-content: 71.4
- method: genome sequence analysis
External links
@ref: 15008
culture collection no.: DSM 53339, NCCB 100640, Soce 321
straininfo link
- @ref: 81436
- straininfo: 216479
literature
- topic: Phylogeny
- Pubmed-ID: 30234476
- title: A polyphasic approach leads to seven new species of the cellulose-decomposing genus Sorangium, Sorangium ambruticinum sp. nov., Sorangium arenae sp. nov., Sorangium bulgaricum sp. nov., Sorangium dawidii sp. nov., Sorangium kenyense sp. nov., Sorangium orientale sp. nov. and Sorangium reichenbachii sp. nov.
- authors: Mohr KI, Wolf C, Nubel U, Szafranska AK, Steglich M, Hennessen F, Gemperlein K, Kampfer P, Martin K, Muller R, Wink J
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.003034
- year: 2018
- mesh: Bacterial Typing Techniques, Cellulose/*metabolism, DNA, Bacterial/genetics, Genes, Bacterial, Myxococcales/*classification, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
- topic2: Transcriptome
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
15008 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 53339) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-53339 | |||
18281 | Reichenbach, H. | Collection description and documentation of myxobacteria by H. Reichenbach, HZI (formerly GBF); Collection curr. located at the DSMZ | ||||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
66744 | Kathrin I. Mohr, Corinna Wolf, Ulrich Nübel, Anna K. Szafranska, Matthias Steglich, Fabienne Hennessen, Katja Gemperlein, Peter Kaempfer, Karin Martin, Rolf Möller and Joachim Wink | 10.1099/ijsem.0.003034 | A polyphasic approach leads to seven new species of the cellulose-decomposing genus Sorangium, Sorangium ambruticinum sp. nov., Sorangium arenae sp. nov., Sorangium bulgaricum sp. nov., Sorangium dawidii sp. nov., Sorangium kenyense sp. nov., Sorangium orientale sp. nov. and Sorangium reichenbachii sp. nov. | IJSEM 68: 3576-3586 2018 | 30234476 | |
81436 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID216479.1 | StrainInfo: A central database for resolving microbial strain identifiers |