Strain identifier
BacDive ID: 1218
Type strain: 
Species: Fictibacillus gelatini
Strain Designation: R-13853
Strain history: <- D. Janssens, LMG <- E. De Clerck; R-13853
NCBI tax ID(s): 1121091 (strain), 225985 (species)
version 8.1 (current version)
General
@ref: 6127
BacDive-ID: 1218
DSM-Number: 15865
keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, thermophilic, Gram-variable, motile, rod-shaped
description: Fictibacillus gelatini R-13853 is an aerobe, spore-forming, thermophilic bacterium that was isolated from gelatin.
NCBI tax id
| NCBI tax id | Matching level |
|---|---|
| 225985 | species |
| 1121091 | strain |
strain history
- @ref: 6127
- history: <- D. Janssens, LMG <- E. De Clerck; R-13853
doi: 10.13145/bacdive1218.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Bacillaceae
- genus: Fictibacillus
- species: Fictibacillus gelatini
- full scientific name: Fictibacillus gelatini (De Clerck et al. 2004) Glaeser et al. 2013
synonyms
- @ref: 20215
- synonym: Bacillus gelatini
@ref: 6127
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Bacillaceae
genus: Fictibacillus
species: Fictibacillus gelatini
full scientific name: Fictibacillus gelatini (De Clerck et al. 2004) Glaeser et al. 2013
strain designation: R-13853
type strain: yes
Morphology
cell morphology
| @ref | gram stain | cell length | cell width | cell shape | motility | confidence |
|---|---|---|---|---|---|---|
| 30118 | variable | 7 µm | 0.7 µm | rod-shaped | yes | |
| 69480 | yes | 96.679 | ||||
| 69480 | positive | 100 |
pigmentation
- @ref: 30118
- production: no
Culture and growth conditions
culture medium
- @ref: 6127
- name: CASO AGAR (MERCK 105458) (DSMZ Medium 220)
- growth: yes
- link: https://mediadive.dsmz.de/medium/220
- composition: Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 6127 | positive | growth | 50 | thermophilic |
| 30118 | positive | optimum | 45 | thermophilic |
culture pH
| @ref | ability | type | pH | PH range |
|---|---|---|---|---|
| 30118 | positive | growth | 4.5-9.5 | alkaliphile |
| 30118 | positive | optimum | 6.5 |
Physiology and metabolism
oxygen tolerance
- @ref: 30118
- oxygen tolerance: aerobe
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 30118 | yes | |
| 69481 | yes | 100 |
| 69480 | yes | 100 |
halophily
- @ref: 30118
- salt: NaCl
- growth: positive
- tested relation: optimum
- concentration: 15 %
observation
- @ref: 30118
- observation: aggregates in chains
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 30118 | 58143 | 5-dehydro-D-gluconate | + | carbon source |
| 30118 | 17057 | cellobiose | + | carbon source |
| 30118 | 28757 | fructose | + | carbon source |
| 30118 | 28260 | galactose | + | carbon source |
| 30118 | 5291 | gelatin | + | carbon source |
| 30118 | 17234 | glucose | + | carbon source |
| 30118 | 17754 | glycerol | + | carbon source |
| 30118 | 17306 | maltose | + | carbon source |
| 30118 | 29864 | mannitol | + | carbon source |
| 30118 | 37684 | mannose | + | carbon source |
| 30118 | 37657 | methyl D-glucoside | + | carbon source |
| 30118 | 506227 | N-acetylglucosamine | + | carbon source |
| 30118 | 33942 | ribose | + | carbon source |
| 30118 | 27082 | trehalose | + | carbon source |
| 30118 | 18222 | xylose | + | carbon source |
| 30118 | 4853 | esculin | + | hydrolysis |
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 30118 | catalase | + | 1.11.1.6 |
| 30118 | gelatinase | + |
Isolation, sampling and environmental information
isolation
- @ref: 6127
- sample type: gelatin
- country: Belgium
- origin.country: BEL
- continent: Europe
isolation source categories
- Cat1: #Engineered
- Cat2: #Industrial
- Cat3: #Engineered product
taxonmaps
- @ref: 69479
- File name: preview.99_43356.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_20006;97_24855;98_31485;99_43356&stattab=map
- Last taxonomy: Fictibacillus gelatini subclade
- 16S sequence: AJ551329
- Sequence Identity:
- Total samples: 612
- soil counts: 225
- aquatic counts: 132
- animal counts: 198
- plant counts: 57
Safety information
risk assessment
- @ref: 6127
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 6127
- description: Bacillus gelatini partial 16S rRNA gene, type strain LMG 21880T
- accession: AJ551329
- length: 1485
- database: ena
- NCBI tax ID: 225985
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Fictibacillus gelatini DSM 15865 | GCA_000430745 | scaffold | ncbi | 1121091 |
| 66792 | Bacillus gelatini DSM 15865 | 1121091.3 | wgs | patric | 1121091 |
| 66792 | Fictibacillus gelatini DSM 15865 | 2523533616 | draft | img | 1121091 |
GC content
- @ref: 30118
- GC-content: 41.5
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 100 | no |
| motile | yes | 86.773 | no |
| flagellated | yes | 74.018 | no |
| gram-positive | yes | 88.914 | no |
| anaerobic | no | 98.773 | yes |
| aerobic | yes | 84.936 | no |
| halophile | no | 87.861 | no |
| spore-forming | yes | 97.654 | no |
| glucose-util | yes | 92.099 | no |
| thermophile | yes | 58.487 | yes |
| glucose-ferment | no | 93.468 | no |
External links
@ref: 6127
culture collection no.: DSM 15865, LMG 21880
straininfo link
- @ref: 70874
- straininfo: 97803
literature
- topic: Phylogeny
- Pubmed-ID: 15143046
- title: Anoxybacillus contaminans sp. nov. and Bacillus gelatini sp. nov., isolated from contaminated gelatin batches.
- authors: De Clerck E, Rodriguez-Diaz M, Vanhoutte T, Heyrman J, Logan NA, De Vos P
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.02960-0
- year: 2004
- mesh: Bacillaceae/*classification/genetics/*isolation & purification/metabolism, Bacillus/*classification/genetics/*isolation & purification/metabolism, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, *Drug Contamination, *Gelatin, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics
- topic2: Enzymology
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
|---|---|---|---|---|---|---|
| 6127 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15865) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-15865 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 30118 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 26473 | 28776041 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
| 70874 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID97803.1 | StrainInfo: A central database for resolving microbial strain identifiers |