Strain identifier

BacDive ID: 1212

Type strain: Yes

Species: Lederbergia galactosidilytica

Strain Designation: MB800

Strain history: CIP <- 2004, DSMZ <- 1997, P. de Vos, Ghent Univ., Ghent, Belgium <- 1997, M. Heyndrickx, APQCA Res., Ghent, Belgium: strain MB800

NCBI tax ID(s): 217031 (species)

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General

@ref: 5910

BacDive-ID: 1212

DSM-Number: 15595

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped, colony-forming

description: Lederbergia galactosidilytica MB800 is a facultative anaerobe, spore-forming, mesophilic bacterium that forms irregular colonies and was isolated from raw milk.

NCBI tax id

  • NCBI tax id: 217031
  • Matching level: species

strain history

@refhistory
5910<- M. Heyndrickx; MB800 <- H. Lieve, DVC-CLO, Belgium
119504CIP <- 2004, DSMZ <- 1997, P. de Vos, Ghent Univ., Ghent, Belgium <- 1997, M. Heyndrickx, APQCA Res., Ghent, Belgium: strain MB800

doi: 10.13145/bacdive1212.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Lederbergia
  • species: Lederbergia galactosidilytica
  • full scientific name: Lederbergia galactosidilytica corrig. (Heyndrickx et al. 2004) Gupta et al. 2020

  • synonyms

    @refsynonym
    20215Bacillus galactosidilyticus
    20215Lederbergia galactosidilyticus

@ref: 5910

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Lederbergia

species: Lederbergia galactosidilytica

full scientific name: Lederbergia galactosidilytica (Heyndrickx et al. 2004) Gupta et al. 2020

strain designation: MB800

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
23311positive2-5 µm0.7-0.9 µmrod-shapedyes
119504positiverod-shapedyes

colony morphology

@refcolony sizecolony colorcolony shapeincubation periodmedium used
233111 mmcreamy, off-white, opaqueirregular2 daysTSA
119504

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5910CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
23311Trypticase Soy Agar (TSA)yes
38177MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
119504CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
5910positivegrowth30mesophilic
23311positivegrowth30mesophilic
23311positivegrowth40thermophilic
23311nogrowth50thermophilic
38177positivegrowth30mesophilic
119504positivegrowth22-45
119504nogrowth10psychrophilic
119504nogrowth55thermophilic

culture pH

@refabilitytypepHPH range
23311positivegrowth6-10.5alkaliphile
23311nogrowth<5
119504positivegrowth6

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
23311facultative anaerobe
119504obligate aerobe

spore formation

@refspore descriptiontype of sporespore formation
23311ellipsoidal, central, paracentral, subterminal, slightly swollen sporangiaendosporeyes
119504yes

halophily

@refsaltgrowthtested relationconcentration
119504NaClpositivegrowth0-8 %
119504NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2331116947citrate-assimilation
2331116467L-arginine-assimilation
2331118019L-lysine-assimilation
2331115729L-ornithine-assimilation
23311168082-dehydro-D-gluconate-builds acid from
23311174265-dehydro-D-gluconate-builds acid from
2331115963ribitol-builds acid from
2331117108D-arabinose-builds acid from
2331118333D-arabitol-builds acid from
2331128847D-fucose-builds acid from
2331162318D-lyxose-builds acid from
2331117924D-sorbitol-builds acid from
2331116443D-tagatose-builds acid from
2331116813galactitol-builds acid from
2331117113erythritol-builds acid from
2331124265gluconate-builds acid from
2331117754glycerol-builds acid from
2331128087glycogen-builds acid from
2331117268myo-inositol-builds acid from
2331118403L-arabitol-builds acid from
2331118287L-fucose-builds acid from
2331117266L-sorbose-builds acid from
2331165328L-xylose-builds acid from
2331143943methyl alpha-D-mannoside-builds acid from
2331174863methyl beta-D-xylopyranoside-builds acid from
2331117151xylitol-builds acid from
2331127897tryptophan-energy source
233115291gelatin-hydrolysis
2331116199urea-hydrolysis
2331190144O-nitrophenyl-beta-D-galactopyranosid+assimilation
2331118305arbutin+builds acid from
2331117057cellobiose+builds acid from
233116731melezitose+builds acid from
2331128053melibiose+builds acid from
2331116634raffinose+builds acid from
2331117992sucrose+builds acid from
2331116551D-trehalose+builds acid from
2331128017starch+builds acid from
233114853esculin+hydrolysis
2331117632nitrate+reduction
2331127613amygdalin+/-builds acid from
2331115824D-fructose+/-builds acid from
2331112936D-galactose+/-builds acid from
2331117634D-glucose+/-builds acid from
2331117716lactose+/-builds acid from
2331117306maltose+/-builds acid from
2331116899D-mannitol+/-builds acid from
2331116024D-mannose+/-builds acid from
2331116988D-ribose+/-builds acid from
2331132528turanose+/-builds acid from
2331165327D-xylose+/-builds acid from
2331128066gentiobiose+/-builds acid from
2331115443inulin+/-builds acid from
2331130849L-arabinose+/-builds acid from
2331162345L-rhamnose+/-builds acid from
23311320061methyl alpha-D-glucopyranoside+/-builds acid from
23311506227N-acetylglucosamine+/-builds acid from
2331117814salicin+/-builds acid from
23311casein+/-hydrolysis
11950416947citrate-carbon source
1195044853esculin+hydrolysis
119504606565hippurate+hydrolysis
11950417632nitrate+reduction
11950416301nitrite-reduction
11950417632nitrate-respiration

metabolite production

@refChebi-IDmetaboliteproduction
2331135581indoleno
2331116136hydrogen sulfideno
2331115688acetoinno
11950435581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole testmethylred-test
2331115688acetoin-
2331135581indole-
11950415688acetoin-
11950417234glucose-

enzymes

@refvalueactivityec
23311catalase+1.11.1.6
23311tryptophan deaminase-4.1.99.1
23311urease+/-3.5.1.5
23311urease-3.5.1.5
68382alpha-fucosidase+3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
119504oxidase+
119504beta-galactosidase-3.2.1.23
119504alcohol dehydrogenase-1.1.1.1
119504gelatinase-
119504amylase-
119504DNase-
119504caseinase+3.4.21.50
119504catalase+1.11.1.6
119504tween esterase-
119504gamma-glutamyltransferase-2.3.2.2
119504lecithinase-
119504lipase-
119504lysine decarboxylase-4.1.1.18
119504ornithine decarboxylase-4.1.1.17
119504urease+3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119504--+---------++-----+

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119504------------------++------------------------------------------------------------------+------------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
5910raw milkBelgiumBELEurope
23311aerobic sporeforming flora of raw milk
119504Food, Raw milk after heat-treatement

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Dairy product
#Environmental#Microbial community
#Host Body Product#Fluids#Milk

taxonmaps

  • @ref: 69479
  • File name: preview.99_2608.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_357;97_1662;98_2020;99_2608&stattab=map
  • Last taxonomy: Lederbergia galactosidilyticus subclade
  • 16S sequence: AJ535638
  • Sequence Identity:
  • Total samples: 977
  • soil counts: 412
  • aquatic counts: 93
  • animal counts: 390
  • plant counts: 82

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
59101Risk group (German classification)
1195041Risk group (French classification)

Sequence information

16S sequences

  • @ref: 5910
  • description: Bacillus galactosidilyticus partial 16S rRNA gene, type strain LMG 17892T
  • accession: AJ535638
  • length: 1486
  • database: ena
  • NCBI tax ID: 217031

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lederbergia galactosidilytica DSM 15595GCA_017873565contigncbi217031
66792Lederbergia galactosidilyticus strain DSM 15595217031.9wgspatric217031
66792Bacillus galactosidilyticus DSM 155952913365302draftimg217031

GC content

@refGC-contentmethod
591037.7
2331137.7high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes87.256no
gram-positiveyes87.088no
anaerobicno98.607yes
aerobicyes75.317yes
halophileyes75.181no
spore-formingyes95.337yes
glucose-utilyes88.133no
thermophileno99.361no
flagellatedyes82.07no
glucose-fermentno84.124no

External links

@ref: 5910

culture collection no.: DSM 15595, LMG 17892, CIP 108417

straininfo link

  • @ref: 70868
  • straininfo: 10694

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny15023985Bacillus galactosidilyticus sp. nov., an alkali-tolerant beta-galactosidase producer.Heyndrickx M, Logan NA, Lebbe L, Rodriguez-Diaz M, Forsyth G, Goris J, Scheldeman P, De Vos PInt J Syst Evol Microbiol10.1099/ijs.0.02816-02004Bacillus/*classification/enzymology/genetics/physiology, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Restriction Mapping, Spores, Bacterial/cytology, beta-Galactosidase/*metabolismGenetics
Phylogeny20656804Bacillus graminis sp. nov., an endophyte isolated from a coastal dune plant.Bibi F, Chung EJ, Jeon CO, Chung YRInt J Syst Evol Microbiol10.1099/ijs.0.023820-02010Bacillus/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Elymus/*microbiology, Endophytes/classification/genetics/isolation & purification, Fatty Acids/chemistry, Molecular Sequence Data, Peptidoglycan/chemistry, *Phylogeny, Plant Roots/microbiology, RNA, Ribosomal, 16S/genetics, Republic of Korea, Salt-Tolerant Plants/microbiology, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistryEnzymology
Phylogeny25825245Bacillus wuyishanensis sp. nov., isolated from rhizosphere soil of a medical plant, Prunella vulgaris.Liu B, Liu GH, Sengonca C, Schumann P, Che JM, Zhu YJ, Wang JPInt J Syst Evol Microbiol10.1099/ijs.0.0002152015Bacillus/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, *Phylogeny, Prunella/*microbiology, RNA, Ribosomal, 16S/genetics, *Rhizosphere, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistryGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5910Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15595)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15595
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23311Marc Heyndrickx, Niall A. Logan, Liesbeth Lebbe, Marina Rodríguez-Díaz, Gillian Forsyth, Johan Goris, Patsy Scheldeman, Paul De Vos10.1099/ijs.0.02816-0Bacillus galactosidilyticus sp. nov., an alkali-tolerant beta-galactosidase producerIJSEM 54: 617-621 200415023985
38177Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6037
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
70868Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID10694.1StrainInfo: A central database for resolving microbial strain identifiers
119504Curators of the CIPCollection of Institut Pasteur (CIP 108417)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108417