Strain identifier
BacDive ID: 1209
Type strain:
Species: Niallia nealsonii
Strain Designation: Fo-92
Strain history: <- M. Satomi; Fo-92
NCBI tax ID(s): 115979 (species)
General
@ref: 5699
BacDive-ID: 1209
DSM-Number: 15077
keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped, colony-forming
description: Niallia nealsonii Fo-92 is a facultative anaerobe, spore-forming, mesophilic bacterium that forms irregular colonies and was isolated from natural fall out particle of the clean room air.
NCBI tax id
- NCBI tax id: 115979
- Matching level: species
strain history: <- M. Satomi; Fo-92
doi: 10.13145/bacdive1209.20221219.7.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Bacillaceae
- genus: Niallia
- species: Niallia nealsonii
- full scientific name: Niallia nealsonii (Venkateswaran et al. 2003) Gupta et al. 2020
synonyms
- @ref: 20215
- synonym: Bacillus nealsonii
@ref: 5699
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Bacillaceae
genus: Niallia
species: Niallia nealsonii
full scientific name: Niallia nealsonii (Venkateswaran et al. 2003) Gupta et al. 2020
strain designation: Fo-92
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility | confidence |
---|---|---|---|---|---|---|
23309 | positive | 4-5 µm | 1 µm | rod-shaped | yes | |
69480 | yes | 88.119 | ||||
69480 | positive | 94.304 |
colony morphology
- @ref: 23309
- colony size: 3-4 mm
- colony color: beige
- colony shape: irregular
- incubation period: 3-4 days
- medium used: TSA
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
5699 | CASO AGAR (MERCK 105458) (DSMZ Medium 220) | yes | https://mediadive.dsmz.de/medium/220 | Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water |
23309 | Trypticase Soy Agar (TSA) | yes | ||
33219 | Marine agar (MA) | yes | Distilled water make up to (1000.000 ml);Marine agar (55.100 g) |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
5699 | positive | growth | 30 | mesophilic |
23309 | positive | growth | 25-60 | |
23309 | positive | optimum | 30-35 | mesophilic |
33219 | positive | growth | 30 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
23309 | positive | growth | 6-10 | alkaliphile |
23309 | positive | optimum | 7 |
Physiology and metabolism
oxygen tolerance
- @ref: 23309
- oxygen tolerance: facultative anaerobe
spore formation
@ref | type of spore | spore formation | confidence |
---|---|---|---|
23309 | endospore | yes | |
69480 | yes | 97.308 |
halophily
@ref | salt | growth | tested relation | concentration | halophily level | confidence |
---|---|---|---|---|---|---|
23309 | NaCl | positive | growth | 0-8 % | ||
69480 | non-halophilic | 86.794 |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
23309 | 27613 | amygdalin | - | assimilation |
23309 | 29864 | mannitol | - | assimilation |
23309 | 15963 | ribitol | - | fermentation |
23309 | 28087 | glycogen | - | fermentation |
23309 | 33942 | ribose | - | fermentation |
23309 | 5291 | gelatin | - | hydrolysis |
23309 | 17632 | nitrate | - | reduction |
23309 | 16808 | 2-dehydro-D-gluconate | + | fermentation |
23309 | 18333 | D-arabitol | + | fermentation |
23309 | 62318 | D-lyxose | + | fermentation |
23309 | 16443 | D-tagatose | + | fermentation |
23309 | 32528 | turanose | + | fermentation |
23309 | 65327 | D-xylose | + | fermentation |
23309 | 28260 | galactose | + | fermentation |
23309 | 28066 | gentiobiose | + | fermentation |
23309 | 24265 | gluconate | + | fermentation |
23309 | 17754 | glycerol | + | fermentation |
23309 | 17268 | myo-inositol | + | fermentation |
23309 | 17716 | lactose | + | fermentation |
23309 | 6731 | melezitose | + | fermentation |
23309 | 28053 | melibiose | + | fermentation |
23309 | 506227 | N-acetylglucosamine | + | fermentation |
23309 | 16634 | raffinose | + | fermentation |
23309 | 26546 | rhamnose | + | fermentation |
23309 | 30911 | sorbitol | + | fermentation |
23309 | 28017 | starch | + | fermentation |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
23309 | 16136 | hydrogen sulfide | no |
23309 | 15688 | acetoin | no |
enzymes
@ref | value | activity | ec |
---|---|---|---|
23309 | alginase | - | 3.2.1.16 |
23309 | amylase | - | |
23309 | arginine dihydrolase | - | 3.5.3.6 |
23309 | beta-galactosidase | + | 3.2.1.23 |
23309 | catalase | + | 1.11.1.6 |
23309 | cytochrome oxidase | - | 1.9.3.1 |
23309 | gelatinase | - | |
23309 | lipase | - | |
23309 | lysine decarboxylase | - | 4.1.1.18 |
23309 | ornithine decarboxylase | - | 4.1.1.17 |
Isolation, sampling and environmental information
isolation
- @ref: 5699
- sample type: natural fall out particle of the clean room air
- geographic location: California, Spacecraft assembly facility
- country: USA
- origin.country: USA
- continent: North America
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Built environment | #Clean room |
#Environmental | #Air | #Indoor Air |
Safety information
risk assessment
- @ref: 5699
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Bacillus nealsonii strain DSM 15077 16S ribosomal RNA gene, partial sequence | EU656111 | 1509 | ena | 115979 |
23309 | Bacillus nealsonii strain FO-092 16S ribosomal RNA gene, partial sequence | AF234863 | 1215 | nuccore | 115979 |
Genome sequences
- @ref: 66792
- description: Niallia nealsonii FO-92
- accession: GCA_002835805
- assembly level: contig
- database: ncbi
- NCBI tax ID: 115979
External links
@ref: 5699
culture collection no.: DSM 15077, ATCC BAA 519
straininfo link
@ref | passport |
---|---|
20218 | http://www.straininfo.net/strains/301997 |
20218 | http://www.straininfo.net/strains/301996 |
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 12656168 | Bacillus nealsonii sp. nov., isolated from a spacecraft-assembly facility, whose spores are gamma-radiation resistant. | Venkateswaran K, Kempf M, Chen F, Satomi M, Nicholson W, Kern R | Int J Syst Evol Microbiol | 10.1099/ijs.0.02311-0 | 2003 | Bacillus/genetics/*isolation & purification/*radiation effects, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Disinfection, Fatty Acids/analysis, Gamma Rays, Microscopy, Electron, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Radiation Tolerance, Spacecraft, Spores, Bacterial/genetics/radiation effects, Ultraviolet Rays, United States, United States National Aeronautics and Space Administration | Genetics |
Phylogeny | 19542113 | Bacillus korlensis sp. nov., a moderately halotolerant bacterium isolated from a sand soil sample in China. | Zhang L, Wang Y, Dai J, Tang Y, Yang Q, Luo X, Fang C | Int J Syst Evol Microbiol | 10.1099/ijs.0.004879-0 | 2009 | Bacillus/*classification/genetics/isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/analysis, Fatty Acids/analysis, Genes, rRNA, Genotype, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, *Salt Tolerance, Sequence Analysis, DNA, *Silicon Dioxide, Sodium Chloride, *Soil Microbiology, Species Specificity | Genetics |
Phylogeny | 31613744 | Bacillus taxi sp. nov., a novel endophytic bacterium isolated from root of Taxus chinensis (Pilger) Rehd. | Tuo L, Liu F, Yan XR, Liu Y | Int J Syst Evol Microbiol | 10.1099/ijsem.0.003776 | 2020 | Bacillus/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Endophytes/classification/isolation & purification, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Plant Roots/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Taxus/*microbiology, Vitamin K 2/analogs & derivatives/chemistry | Enzymology |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
5699 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15077) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-15077 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
23309 | Kasthuri Venkateswaran, Michael Kempf, Fei Chen, Masataka Satomi, Wayne Nicholson, Roger Kern | 10.1099/ijs.0.02311-0 | Bacillus nealsonii sp. nov., isolated from a spacecraft-assembly facility, whose spores are gamma-radiation resistant | IJSEM 53: 165-172 2003 | 12656168 | |
33219 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/5865 | ||||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) |