Strain identifier

BacDive ID: 1209

Type strain: Yes

Species: Niallia nealsonii

Strain Designation: Fo-92

Strain history: CIP <- 2004, K. Venkateswaran, Pasadena, USA: strain FO-92

NCBI tax ID(s): 115979 (species)

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General

@ref: 5699

BacDive-ID: 1209

DSM-Number: 15077

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped, colony-forming

description: Niallia nealsonii Fo-92 is a facultative anaerobe, spore-forming, mesophilic bacterium that forms irregular colonies and was isolated from natural fall out particle of the clean room air.

NCBI tax id

  • NCBI tax id: 115979
  • Matching level: species

strain history

@refhistory
5699<- M. Satomi; Fo-92
121247CIP <- 2004, K. Venkateswaran, Pasadena, USA: strain FO-92

doi: 10.13145/bacdive1209.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Niallia
  • species: Niallia nealsonii
  • full scientific name: Niallia nealsonii (Venkateswaran et al. 2003) Gupta et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Bacillus nealsonii

@ref: 5699

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Niallia

species: Niallia nealsonii

full scientific name: Niallia nealsonii (Venkateswaran et al. 2003) Gupta et al. 2020

strain designation: Fo-92

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
23309positive4-5 µm1 µmrod-shapedyes
121247positiverod-shapedno

colony morphology

@refcolony sizecolony colorcolony shapeincubation periodmedium used
233093-4 mmbeigeirregular3-4 daysTSA
121247

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5699CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
23309Trypticase Soy Agar (TSA)yes
33219Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
121247CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
121247CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperaturerange
5699positivegrowth30mesophilic
23309positivegrowth25-60
23309positiveoptimum30-35mesophilic
33219positivegrowth30mesophilic
121247positivegrowth22-37
121247nogrowth10psychrophilic
121247nogrowth45thermophilic
121247nogrowth55thermophilic

culture pH

@refabilitytypepHPH range
23309positivegrowth6-10alkaliphile
23309positiveoptimum7
121247positivegrowth6

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
23309facultative anaerobe
121247facultative anaerobe

spore formation

@reftype of sporespore formation
23309endosporeyes
121247yes

halophily

@refsaltgrowthtested relationconcentration
23309NaClpositivegrowth0-8 %
121247NaClpositivegrowth0-4 %
121247NaClnogrowth6 %
121247NaClnogrowth8 %
121247NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2330927613amygdalin-assimilation
2330929864mannitol-assimilation
2330915963ribitol-fermentation
2330928087glycogen-fermentation
2330933942ribose-fermentation
233095291gelatin-hydrolysis
2330917632nitrate-reduction
23309168082-dehydro-D-gluconate+fermentation
2330918333D-arabitol+fermentation
2330962318D-lyxose+fermentation
2330916443D-tagatose+fermentation
2330932528turanose+fermentation
2330965327D-xylose+fermentation
2330928260galactose+fermentation
2330928066gentiobiose+fermentation
2330924265gluconate+fermentation
2330917754glycerol+fermentation
2330917268myo-inositol+fermentation
2330917716lactose+fermentation
233096731melezitose+fermentation
2330928053melibiose+fermentation
23309506227N-acetylglucosamine+fermentation
2330916634raffinose+fermentation
2330926546rhamnose+fermentation
2330930911sorbitol+fermentation
2330928017starch+fermentation
68371Potassium 5-ketogluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose+builds acid from
6837132528turanose+builds acid from
6837128066gentiobiose+builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose+builds acid from
683716731melezitose+builds acid from
6837115443inulin-builds acid from
6837127082trehalose+builds acid from
6837117992sucrose+builds acid from
6837128053melibiose+builds acid from
6837117716lactose+builds acid from
6837117306maltose+builds acid from
6837117057cellobiose+builds acid from
6837117814salicin+builds acid from
683714853esculin+builds acid from
6837118305arbutin+builds acid from
6837127613amygdalin+builds acid from
6837159640N-acetylglucosamine+builds acid from
68371320061methyl alpha-D-glucopyranoside+builds acid from
6837117924D-sorbitol+builds acid from
6837116899D-mannitol+builds acid from
6837117268myo-inositol+builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose+builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837116988D-ribose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
12124716947citrate-carbon source
1212474853esculin+hydrolysis
121247606565hippurate-hydrolysis
12124717632nitrate-reduction
12124716301nitrite-reduction
12124717632nitrate+respiration

metabolite production

@refChebi-IDmetaboliteproduction
2330916136hydrogen sulfideno
2330915688acetoinno
12124735581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12124715688acetoin-
12124717234glucose+

enzymes

@refvalueactivityec
23309alginase-3.2.1.16
23309amylase-
23309arginine dihydrolase-3.5.3.6
23309beta-galactosidase+3.2.1.23
23309catalase+1.11.1.6
23309cytochrome oxidase-1.9.3.1
23309gelatinase-
23309lipase-
23309lysine decarboxylase-4.1.1.18
23309ornithine decarboxylase-4.1.1.17
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
121247oxidase-
121247beta-galactosidase+3.2.1.23
121247alcohol dehydrogenase-1.1.1.1
121247gelatinase-
121247amylase+
121247DNase+
121247caseinase-3.4.21.50
121247catalase+1.11.1.6
121247tween esterase-
121247gamma-glutamyltransferase-2.3.2.2
121247lecithinase-
121247lipase-
121247lysine decarboxylase-4.1.1.18
121247ornithine decarboxylase-4.1.1.17
121247urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121247-++--++--+++-+-++---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
121247+/---+/--+/---+/-+/-+++-+-++++/-++++++++++++-++---+++/-+----+/-+/--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
121247++++--+++++++++++++---++-++---++-+-++----+--------------+--++--------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
5699natural fall out particle of the clean room airCalifornia, Spacecraft assembly facilityUSAUSANorth America
121247Jet Propulsion Laboratory Spacecraft Assembly FacilityCaliforniaUnited States of AmericaUSANorth America1999

isolation source categories

Cat1Cat2Cat3
#Engineered#Built environment#Clean room
#Environmental#Air#Indoor Air

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
56991Risk group (German classification)
1212471Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Bacillus nealsonii strain DSM 15077 16S ribosomal RNA gene, partial sequenceEU6561111509ena115979
23309Bacillus nealsonii strain FO-092 16S ribosomal RNA gene, partial sequenceAF2348631215nuccore115979

Genome sequences

  • @ref: 66792
  • description: Niallia nealsonii FO-92
  • accession: GCA_002835805
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 115979

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedyes84.861no
gram-positiveyes92.939yes
anaerobicno98.224yes
halophileno69.115no
spore-formingyes94.082yes
thermophileno99.051yes
glucose-utilyes88.905no
aerobicyes76.126no
motileyes87.532no
glucose-fermentno82.387no

External links

@ref: 5699

culture collection no.: DSM 15077, ATCC BAA 519, CIP 108262

straininfo link

  • @ref: 70865
  • straininfo: 100521

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny12656168Bacillus nealsonii sp. nov., isolated from a spacecraft-assembly facility, whose spores are gamma-radiation resistant.Venkateswaran K, Kempf M, Chen F, Satomi M, Nicholson W, Kern RInt J Syst Evol Microbiol10.1099/ijs.0.02311-02003Bacillus/genetics/*isolation & purification/*radiation effects, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Disinfection, Fatty Acids/analysis, Gamma Rays, Microscopy, Electron, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Radiation Tolerance, Spacecraft, Spores, Bacterial/genetics/radiation effects, Ultraviolet Rays, United States, United States National Aeronautics and Space AdministrationGenetics
Phylogeny19542113Bacillus korlensis sp. nov., a moderately halotolerant bacterium isolated from a sand soil sample in China.Zhang L, Wang Y, Dai J, Tang Y, Yang Q, Luo X, Fang CInt J Syst Evol Microbiol10.1099/ijs.0.004879-02009Bacillus/*classification/genetics/isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/analysis, Fatty Acids/analysis, Genes, rRNA, Genotype, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, *Salt Tolerance, Sequence Analysis, DNA, *Silicon Dioxide, Sodium Chloride, *Soil Microbiology, Species SpecificityGenetics
Phylogeny31613744Bacillus taxi sp. nov., a novel endophytic bacterium isolated from root of Taxus chinensis (Pilger) Rehd.Tuo L, Liu F, Yan XR, Liu YInt J Syst Evol Microbiol10.1099/ijsem.0.0037762020Bacillus/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Endophytes/classification/isolation & purification, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Plant Roots/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Taxus/*microbiology, Vitamin K 2/analogs & derivatives/chemistryEnzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5699Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15077)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15077
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
23309Kasthuri Venkateswaran, Michael Kempf, Fei Chen, Masataka Satomi, Wayne Nicholson, Roger Kern10.1099/ijs.0.02311-0Bacillus nealsonii sp. nov., isolated from a spacecraft-assembly facility, whose spores are gamma-radiation resistantIJSEM 53: 165-172 200312656168
33219Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5865
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
70865Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID100521.1StrainInfo: A central database for resolving microbial strain identifiers
121247Curators of the CIPCollection of Institut Pasteur (CIP 108262)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108262