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Strain identifier

BacDive ID: 1209

Type strain: Yes

Species: Niallia nealsonii

Strain Designation: Fo-92

Strain history: <- M. Satomi; Fo-92

NCBI tax ID(s): 115979 (species)

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General

@ref: 5699

BacDive-ID: 1209

DSM-Number: 15077

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped, colony-forming

description: Niallia nealsonii Fo-92 is a facultative anaerobe, spore-forming, mesophilic bacterium that forms irregular colonies and was isolated from natural fall out particle of the clean room air.

NCBI tax id

  • NCBI tax id: 115979
  • Matching level: species

strain history: <- M. Satomi; Fo-92

doi: 10.13145/bacdive1209.20221219.7.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Niallia
  • species: Niallia nealsonii
  • full scientific name: Niallia nealsonii (Venkateswaran et al. 2003) Gupta et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Bacillus nealsonii

@ref: 5699

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Niallia

species: Niallia nealsonii

full scientific name: Niallia nealsonii (Venkateswaran et al. 2003) Gupta et al. 2020

strain designation: Fo-92

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
23309positive4-5 µm1 µmrod-shapedyes
69480yes88.119
69480positive94.304

colony morphology

  • @ref: 23309
  • colony size: 3-4 mm
  • colony color: beige
  • colony shape: irregular
  • incubation period: 3-4 days
  • medium used: TSA

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5699CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
23309Trypticase Soy Agar (TSA)yes
33219Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)

culture temp

@refgrowthtypetemperaturerange
5699positivegrowth30mesophilic
23309positivegrowth25-60
23309positiveoptimum30-35mesophilic
33219positivegrowth30mesophilic

culture pH

@refabilitytypepHPH range
23309positivegrowth6-10alkaliphile
23309positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 23309
  • oxygen tolerance: facultative anaerobe

spore formation

@reftype of sporespore formationconfidence
23309endosporeyes
69480yes97.308

halophily

@refsaltgrowthtested relationconcentrationhalophily levelconfidence
23309NaClpositivegrowth0-8 %
69480non-halophilic86.794

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2330927613amygdalin-assimilation
2330929864mannitol-assimilation
2330915963ribitol-fermentation
2330928087glycogen-fermentation
2330933942ribose-fermentation
233095291gelatin-hydrolysis
2330917632nitrate-reduction
23309168082-dehydro-D-gluconate+fermentation
2330918333D-arabitol+fermentation
2330962318D-lyxose+fermentation
2330916443D-tagatose+fermentation
2330932528turanose+fermentation
2330965327D-xylose+fermentation
2330928260galactose+fermentation
2330928066gentiobiose+fermentation
2330924265gluconate+fermentation
2330917754glycerol+fermentation
2330917268myo-inositol+fermentation
2330917716lactose+fermentation
233096731melezitose+fermentation
2330928053melibiose+fermentation
23309506227N-acetylglucosamine+fermentation
2330916634raffinose+fermentation
2330926546rhamnose+fermentation
2330930911sorbitol+fermentation
2330928017starch+fermentation

metabolite production

@refChebi-IDmetaboliteproduction
2330916136hydrogen sulfideno
2330915688acetoinno

enzymes

@refvalueactivityec
23309alginase-3.2.1.16
23309amylase-
23309arginine dihydrolase-3.5.3.6
23309beta-galactosidase+3.2.1.23
23309catalase+1.11.1.6
23309cytochrome oxidase-1.9.3.1
23309gelatinase-
23309lipase-
23309lysine decarboxylase-4.1.1.18
23309ornithine decarboxylase-4.1.1.17

Isolation, sampling and environmental information

isolation

  • @ref: 5699
  • sample type: natural fall out particle of the clean room air
  • geographic location: California, Spacecraft assembly facility
  • country: USA
  • origin.country: USA
  • continent: North America

isolation source categories

Cat1Cat2Cat3
#Engineered#Built environment#Clean room
#Environmental#Air#Indoor Air

Safety information

risk assessment

  • @ref: 5699
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Bacillus nealsonii strain DSM 15077 16S ribosomal RNA gene, partial sequenceEU6561111509ena115979
23309Bacillus nealsonii strain FO-092 16S ribosomal RNA gene, partial sequenceAF2348631215nuccore115979

Genome sequences

  • @ref: 66792
  • description: Niallia nealsonii FO-92
  • accession: GCA_002835805
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 115979

External links

@ref: 5699

culture collection no.: DSM 15077, ATCC BAA 519

straininfo link

@refpassport
20218http://www.straininfo.net/strains/301997
20218http://www.straininfo.net/strains/301996

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny12656168Bacillus nealsonii sp. nov., isolated from a spacecraft-assembly facility, whose spores are gamma-radiation resistant.Venkateswaran K, Kempf M, Chen F, Satomi M, Nicholson W, Kern RInt J Syst Evol Microbiol10.1099/ijs.0.02311-02003Bacillus/genetics/*isolation & purification/*radiation effects, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Disinfection, Fatty Acids/analysis, Gamma Rays, Microscopy, Electron, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Radiation Tolerance, Spacecraft, Spores, Bacterial/genetics/radiation effects, Ultraviolet Rays, United States, United States National Aeronautics and Space AdministrationGenetics
Phylogeny19542113Bacillus korlensis sp. nov., a moderately halotolerant bacterium isolated from a sand soil sample in China.Zhang L, Wang Y, Dai J, Tang Y, Yang Q, Luo X, Fang CInt J Syst Evol Microbiol10.1099/ijs.0.004879-02009Bacillus/*classification/genetics/isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/analysis, Fatty Acids/analysis, Genes, rRNA, Genotype, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, *Salt Tolerance, Sequence Analysis, DNA, *Silicon Dioxide, Sodium Chloride, *Soil Microbiology, Species SpecificityGenetics
Phylogeny31613744Bacillus taxi sp. nov., a novel endophytic bacterium isolated from root of Taxus chinensis (Pilger) Rehd.Tuo L, Liu F, Yan XR, Liu YInt J Syst Evol Microbiol10.1099/ijsem.0.0037762020Bacillus/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Endophytes/classification/isolation & purification, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Plant Roots/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Taxus/*microbiology, Vitamin K 2/analogs & derivatives/chemistryEnzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5699Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15077)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15077
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
23309Kasthuri Venkateswaran, Michael Kempf, Fei Chen, Masataka Satomi, Wayne Nicholson, Roger Kern10.1099/ijs.0.02311-0Bacillus nealsonii sp. nov., isolated from a spacecraft-assembly facility, whose spores are gamma-radiation resistantIJSEM 53: 165-172 200312656168
33219Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5865
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)