Strain identifier

BacDive ID: 12006

Type strain: Yes

Species: Sporosarcina newyorkensis

Strain Designation: 6062

Strain history: <- W. J. Wolfgang, Wadsworth Center, NYS Dept. Health, USA; strain 6062 <- Sisters of Charity Hospital, Catholic Health System

NCBI tax ID(s): 759851 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 17272

BacDive-ID: 12006

DSM-Number: 23544

keywords: 16S sequence, Bacteria, facultative anaerobe, spore-forming, Gram-positive, motile, rod-shaped

description: Sporosarcina newyorkensis 6062 is a facultative anaerobe, spore-forming, Gram-positive bacterium that was isolated from clinical specimen, blood of a 57-year-old man.

NCBI tax id

  • NCBI tax id: 759851
  • Matching level: species

strain history

  • @ref: 17272
  • history: <- W. J. Wolfgang, Wadsworth Center, NYS Dept. Health, USA; strain 6062 <- Sisters of Charity Hospital, Catholic Health System

doi: 10.13145/bacdive12006.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Caryophanaceae
  • genus: Sporosarcina
  • species: Sporosarcina newyorkensis
  • full scientific name: Sporosarcina newyorkensis Wolfgang et al. 2012

@ref: 17272

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Caryophanaceae

genus: Sporosarcina

species: Sporosarcina newyorkensis

full scientific name: Sporosarcina newyorkensis Wolfgang et al. 2012

strain designation: 6062

type strain: yes

Morphology

cell morphology

  • @ref: 30158
  • gram stain: positive
  • cell length: 1.5 µm
  • cell width: 0.6 µm
  • cell shape: rod-shaped
  • motility: yes

pigmentation

  • @ref: 30158
  • production: yes

Culture and growth conditions

culture medium

  • @ref: 17272
  • name: CASO AGAR (MERCK 105458) (DSMZ Medium 220)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/220
  • composition: Name: CASO AGAR (Merck 105458) (DSMZ Medium 220; with strain-specific modifications) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l MnSO4 0.01 g/l Distilled water

culture temp

@refgrowthtypetemperature
17272positivegrowth37
30158positivegrowth10-42
30158positiveoptimum22-28
62055positivegrowth37

culture pH

  • @ref: 30158
  • ability: positive
  • type: optimum
  • pH: 7.2-9.5
  • PH range: alkaliphile

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
30158facultative anaerobe
62055aerobe

spore formation

  • @ref: 30158
  • spore formation: yes

halophily

  • @ref: 30158
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 2-13 %

metabolite utilization

  • @ref: 30158
  • Chebi-ID: 17632
  • metabolite: nitrate
  • utilization activity: +
  • kind of utilization tested: reduction

enzymes

@refvalueactivityec
30158alkaline phosphatase+3.1.3.1
30158alpha-galactosidase+3.2.1.22
30158catalase+1.11.1.6
30158gelatinase+
30158cytochrome oxidase+1.9.3.1
30158urease+3.5.1.5

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
17272clinical specimen, blood of a 57-year-old manNew York State, Erie CountyUSAUSANorth America
62055Human bloodBuffalo,New York StateUSAUSANorth America2008-10-17

isolation source categories

Cat1Cat2Cat3
#Infection#Medical environment#Clinic
#Infection#Patient
#Infection#Patient#Specimen
#Host Body Product#Fluids#Blood
#Host#Human#Male

taxonmaps

  • @ref: 69479
  • File name: preview.99_3308.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_217;97_236;98_2535;99_3308&stattab=map
  • Last taxonomy: Sporosarcina newyorkensis subclade
  • 16S sequence: GU994085
  • Sequence Identity:
  • Total samples: 647
  • soil counts: 150
  • aquatic counts: 72
  • animal counts: 410
  • plant counts: 15

Safety information

risk assessment

  • @ref: 17272
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 17272
  • description: Sporosarcina newyorkensis strain 6062 16S ribosomal RNA gene, partial sequence
  • accession: GU994085
  • length: 1474
  • database: nuccore
  • NCBI tax ID: 759851

GC content

@refGC-contentmethod
1727242.4high performance liquid chromatography (HPLC)
3015842.4

External links

@ref: 17272

culture collection no.: DSM 23544, CCUG 59649, LMG 26022

straininfo link

  • @ref: 81225
  • straininfo: 374122

literature

  • topic: Phylogeny
  • Pubmed-ID: 21421928
  • title: Sporosarcina newyorkensis sp. nov. from clinical specimens and raw cow's milk.
  • authors: Wolfgang WJ, Coorevits A, Cole JA, De Vos P, Dickinson MC, Hannett GE, Jose R, Nazarian EJ, Schumann P, Van Landschoot A, Wirth SE, Musser KA
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.030080-0
  • year: 2011
  • mesh: Adult, Aged, 80 and over, Animals, Bacterial Typing Techniques, Belgium/epidemiology, Cattle, Female, Genes, rRNA, Gram-Positive Bacterial Infections/epidemiology/*microbiology, Gram-Positive Endospore-Forming Rods/classification/genetics/isolation & purification, Humans, Male, Middle Aged, Milk/*microbiology, Molecular Sequence Data, New York/epidemiology, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, RNA, Ribosomal, 23S/genetics, Sequence Analysis, DNA, Species Specificity, Sporosarcina/*classification/genetics/*isolation & purification/physiology
  • topic2: Enzymology

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
17272Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23544)https://www.dsmz.de/collection/catalogue/details/culture/DSM-23544
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
30158Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2650928776041
62055Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 59649)https://www.ccug.se/strain?id=59649
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
81225Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID374122.1StrainInfo: A central database for resolving microbial strain identifiers