Strain identifier

BacDive ID: 12

Type strain: Yes

Species: Acetobacter cerevisiae

Strain history: CIP <- 2002, NCIMB <- LMG <- R. Parsons

NCBI tax ID(s): 1307903 (strain), 178900 (species)

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General

@ref: 5318

BacDive-ID: 12

DSM-Number: 14362

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, rod-shaped, colony-forming

description: Acetobacter cerevisiae DSM 14362 is an obligate aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from beer in storage.

NCBI tax id

NCBI tax idMatching level
178900species
1307903strain

strain history

@refhistory
5318<- D. Janssens, LMG <- NCIB <- R. Parsons
67770LMG 1625 <-- NCIB 8894 <-- R. H. Parsons.
118210CIP <- 2002, NCIMB <- LMG <- R. Parsons

doi: 10.13145/bacdive12.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Rhodospirillales
  • family: Acetobacteraceae
  • genus: Acetobacter
  • species: Acetobacter cerevisiae
  • full scientific name: Acetobacter cerevisiae Cleenwerck et al. 2002

@ref: 5318

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Acetobacteraceae

genus: Acetobacter

species: Acetobacter cerevisiae

full scientific name: Acetobacter cerevisiae Cleenwerck et al. 2002

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
23233negative2.0-4.0 µm1 µmrod-shapedno
69480negative99.99
118210negativerod-shapedno

colony morphology

  • @ref: 23233
  • colony size: 0.3-0.5 mm
  • colony color: beige to brown
  • colony shape: circular
  • medium used: YPM agar

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5318YPM MEDIUM (DSMZ Medium 360)yeshttps://mediadive.dsmz.de/medium/360Name: YPM MEDIUM (DSMZ Medium 360) Composition: Mannitol 25.0 g/l Agar 12.0 g/l Yeast extract 5.0 g/l Peptone 3.0 g/l Distilled water
23233YPM agaryes
41779MEDIUM 314 - for Gluconacetobacter xylinus subsp. sucrofermentansyesDistilled water make up to (1000.000 ml);Agar (15.000 g);Glucose (100.000 g);Yeast extract (10.000 g);Calcium carbonate (30.000 g)
118210CIP Medium 314yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=314

culture temp

@refgrowthtypetemperaturerange
5318positivegrowth28mesophilic
41779positivegrowth30mesophilic
67770positivegrowth30mesophilic
118210positivegrowth22-41
118210nogrowth10psychrophilic
118210nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
23233obligate aerobe
118210obligate aerobe

spore formation

@refspore formationconfidence
23233no
69481no100
69480no99.995

compound production

  • @ref: 23233
  • compound: 2-Dehydro-D-gluconate

halophily

@refsaltgrowthtested relationconcentration
118210NaClpositivegrowth0-2 %
118210NaClnogrowth4 %
118210NaClnogrowth6 %
118210NaClnogrowth8 %
118210NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2323316236ethanol-carbon source
2323317306maltose-growth
2323317790methanol-growth
2323317754glycerol+growth
2323328938ammoniumnitrogen source
118210606565hippurate-hydrolysis
11821017632nitrate-reduction
11821016301nitrite-reduction

antibiotic resistance

  • @ref: 118210
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
23233174265-dehydro-D-gluconateno
23233168082-dehydro-D-gluconateyes
11821035581indoleno

enzymes

@refvalueactivityec
23233cytochrome oxidase-1.9.3.1
23233catalase+1.11.1.6
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
118210oxidase-
118210beta-galactosidase-3.2.1.23
118210gelatinase-
118210catalase+1.11.1.6
118210gamma-glutamyltransferase-2.3.2.2
118210urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
118210--++-++---++--------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
118210-------------------------------------------------------+-------------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
5318beer (ale) in storageTorontoCanadaCANNorth America
67770Beer (ale) in storageTorontoCanadaCANNorth America
118210BeerCanadaCANNorth America

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Fermented
#Engineered#Food production#Beverage

taxonmaps

  • @ref: 69479
  • File name: preview.99_1214.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_29;96_152;97_827;98_961;99_1214&stattab=map
  • Last taxonomy: Acetobacter
  • 16S sequence: JF793954
  • Sequence Identity:
  • Total samples: 1514
  • soil counts: 185
  • aquatic counts: 172
  • animal counts: 932
  • plant counts: 225

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
53181Risk group (German classification)
1182101Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Acetobacter cerevisiae gene for 16S rRNA, partial sequence, strain: JCM 17273AB6650801412ena178900
20218Acetobacter cerevisiae strain LMG 1625 16S ribosomal RNA gene, partial sequenceJF7939541358ena178900
23233Acetobacter cerevisiae 16S rRNA gene, strain LMG 1625AJ4198431442nuccore

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Acetobacter cerevisiae strain LMG 1625178900.5wgspatric178900
66792Acetobacter cerevisiae LMG 16252724679583draftimg178900
67770Acetobacter cerevisiae LMG 1625GCA_001580535contigncbi178900

GC content

@refGC-contentmethod
2323356.0-57.6high performance liquid chromatography (HPLC)
6777057.6high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno87.623yes
gram-positiveno95.961yes
anaerobicno99.361yes
aerobicyes92.863yes
halophileno93.295no
spore-formingno95.422no
thermophileno97.044yes
glucose-utilyes80.239no
flagellatedno97.553yes
glucose-fermentno89.734no

External links

@ref: 5318

culture collection no.: DSM 14362, ATCC 23765, LMG 1625, NCIMB 8894, JCM 17273, CIP 107722, VTT E-042461

straininfo link

  • @ref: 69696
  • straininfo: 38218

literature

  • topic: Phylogeny
  • Pubmed-ID: 12361257
  • title: Re-examination of the genus Acetobacter, with descriptions of Acetobacter cerevisiae sp. nov. and Acetobacter malorum sp. nov.
  • authors: Cleenwerck I, Vandemeulebroecke K, Janssens D, Swings J
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/00207713-52-5-1551
  • year: 2002
  • mesh: Acetobacter/*classification/*genetics/isolation & purification/metabolism, Base Composition, Base Sequence, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Species Specificity, Terminology as Topic
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5318Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14362)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14362
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
23233I. Cleenwerck, K. Vandemeulebroecke, D. Janssens and J. Swings10.1099/ijs.0.02064-0Re-examination of the genus Acetobacter, with descriptions of Acetobacter cerevisiae sp. nov. and Acetobacter malorum sp. nov.IJSEM 52: 1551-1558 2002
41779Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5189
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
69696Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID38218.1StrainInfo: A central database for resolving microbial strain identifiers
118210Curators of the CIPCollection of Institut Pasteur (CIP 107722)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107722