Strain identifier

BacDive ID: 1194

Type strain: Yes

Species: Robertmurraya siralis

Strain Designation: 171544

Strain history: CIP <- 1999, F.C. Priest, Heriot Watt Univ., Edinburgh, UK: strain 171544 <- F. Hembke

NCBI tax ID(s): 77777 (species)

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General

@ref: 4920

BacDive-ID: 1194

DSM-Number: 13140

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped

description: Robertmurraya siralis 171544 is an obligate aerobe, spore-forming, mesophilic bacterium that was isolated from silage.

NCBI tax id

  • NCBI tax id: 77777
  • Matching level: species

strain history

@refhistory
4920<- F. G. Priest, HWU; 171544
67770CIP 106295 <-- F. G. Priest 171544 <-- F. Hembke.
122638CIP <- 1999, F.C. Priest, Heriot Watt Univ., Edinburgh, UK: strain 171544 <- F. Hembke

doi: 10.13145/bacdive1194.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Robertmurraya
  • species: Robertmurraya siralis
  • full scientific name: Robertmurraya siralis (Pettersson et al. 2000) Gupta et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Bacillus siralis

@ref: 4920

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Robertmurraya

species: Robertmurraya siralis

full scientific name: Robertmurraya siralis (Pettersson et al. 2000) Gupta et al. 2020

strain designation: 171544

type strain: yes

Morphology

cell morphology

  • @ref: 122638
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 122638

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4920BHI MEDIUM (DSMZ Medium 215)yeshttps://mediadive.dsmz.de/medium/215Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water
38616MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
122638CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
4920positivegrowth30mesophilic
38616positivegrowth30mesophilic
67770positivegrowth30mesophilic
122638positivegrowth22-45
122638nogrowth10psychrophilic
122638nogrowth55thermophilic

culture pH

  • @ref: 122638
  • ability: no
  • type: growth
  • pH: 6

Physiology and metabolism

oxygen tolerance

  • @ref: 122638
  • oxygen tolerance: obligate aerobe

spore formation

  • @ref: 122638
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
122638NaClpositivegrowth0-6 %
122638NaClnogrowth8 %
122638NaClnogrowth10 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
122638citrate-carbon source16947
122638esculin+hydrolysis4853
122638hippurate-hydrolysis606565
122638nitrate+reduction17632
122638nitrite+reduction16301

metabolite production

  • @ref: 122638
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12263815688acetoin-
12263817234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
122638oxidase+
122638beta-galactosidase-3.2.1.23
122638alcohol dehydrogenase-1.1.1.1
122638gelatinase+
122638amylase-
122638DNase-
122638caseinase+3.4.21.50
122638catalase+1.11.1.6
122638tween esterase-
122638gamma-glutamyltransferase-2.3.2.2
122638lecithinase-
122638lipase-
122638lysine decarboxylase-4.1.1.18
122638ornithine decarboxylase-4.1.1.17
122638protease-
122638urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122638-+++-++-++++---++---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
122638+/---+/-+/-+/-----+++----+---+-+++++---+--------------+/---

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
122638++-++----++----+-+++++---------+-+----------------------+--++--------------+----------+--++---+----

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
4920silageStuttgartGermanyDEUEurope
67770Silage
122638SilageStuttgartGermanyDEUEurope1996

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Animal feed
#Engineered#Biodegradation#Composting
#Host#Plants#Herbaceous plants (Grass,Crops)

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
49201Risk group (German classification)
1226381Risk group (French classification)

Sequence information

16S sequences

  • @ref: 67770
  • description: Bacillus sp. 171544 16S ribosomal RNA gene, partial sequence
  • accession: AF071856
  • length: 1523
  • database: ena
  • NCBI tax ID: 77777

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Bacillus siralis strain 17154477777.5wgspatric77777
67770Robertmurraya siralis 171544GCA_005502275contigncbi77777

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes90.752no
flagellatedyes85.3no
gram-positiveyes84.369no
anaerobicno97.539no
halophileno80.727no
spore-formingyes96.643no
glucose-utilyes88.938no
aerobicyes74.737no
thermophileno95.116yes
glucose-fermentno87.935no

External links

@ref: 4920

culture collection no.: DSM 13140, CIP 106295, NCIMB 13601, JCM 12213

straininfo link

  • @ref: 70851
  • straininfo: 311071

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny11155995Bacillus siralis sp. nov., a novel species from silage with a higher order structural attribute in the 16S rRNA genes.Pettersson B, de Silva SK, Uhlen M, Priest FGInt J Syst Evol Microbiol10.1099/00207713-50-6-21812000Bacillus/*classification/*genetics/physiology, Base Sequence, Culture Media, DNA, Ribosomal/analysis, *Genes, rRNA, Molecular Sequence Data, Phenotype, Phylogeny, Polymerase Chain Reaction, RNA, Ribosomal, 16S/*chemistry/*genetics, Random Amplified Polymorphic DNA Technique, Sequence Analysis, DNA, Silage/*microbiology, Spores, Bacterial/physiologyCultivation
Phylogeny31628627Bacillus yapensis sp. nov., a novel piezotolerant bacterium isolated from deep-sea sediment of the Yap Trench, Pacific Ocean.Xu X, Yu L, Xu G, Wang Q, Wei S, Tang XAntonie Van Leeuwenhoek10.1007/s10482-019-01348-72019Aquatic Organisms, Bacillus/*classification/isolation & purification/physiology, Bacterial Typing Techniques, Geologic Sediments/*microbiology, Pacific Ocean, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiologyPhenotype

Reference

@idauthorscataloguedoi/urltitle
4920Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13140)https://www.dsmz.de/collection/catalogue/details/culture/DSM-13140
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
38616Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18409
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
70851Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID311071.1StrainInfo: A central database for resolving microbial strain identifiers
122638Curators of the CIPCollection of Institut Pasteur (CIP 106295)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106295