Strain identifier

BacDive ID: 11912

Type strain: Yes

Species: Thiomicrospira cyclica

Strain history: <- D. Y. Sorokin; ALM1

NCBI tax ID(s): 147268 (species)

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General

@ref: 5370

BacDive-ID: 11912

DSM-Number: 14477

keywords: 16S sequence, Bacteria, obligate aerobe, chemolithoautotroph, mesophilic, motile

description: Thiomicrospira cyclica DSM 14477 is an obligate aerobe, chemolithoautotroph, mesophilic bacterium that was isolated from water.

NCBI tax id

  • NCBI tax id: 147268
  • Matching level: species

strain history

  • @ref: 5370
  • history: <- D. Y. Sorokin; ALM1

doi: 10.13145/bacdive11912.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Beggiatoales
  • family: Piscirickettsiaceae
  • genus: Thiomicrospira
  • species: Thiomicrospira cyclica
  • full scientific name: Thiomicrospira cyclica (Sorokin et al. 2002) Boden et al. 2017

  • synonyms

    @refsynonym
    20215Thioalkalimicrobium cyclicum
    20215Thialkalimicrobium cyclicum

@ref: 5370

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Thiotrichales

family: Piscirickettsiaceae

genus: Thiomicrospira

species: Thiomicrospira cyclica

full scientific name: Thiomicrospira cyclica (Sorokin et al. 2002) Boden et al. 2017

type strain: yes

Morphology

cell morphology

  • @ref: 43182
  • cell length: 1 µm
  • cell width: 0.3-0.4 µm
  • motility: yes

colony morphology

  • @ref: 43182
  • colony color: reddish
  • medium used: Thiosulfate agar

multimedia

@refmultimedia contentcaptionintellectual property rights
66793EM_DSM_14477_1.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_14477_2.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_14477_3.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_14477_4.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_14477_5.jpgelectron microscopic image© HZI/Manfred Rohde

Culture and growth conditions

culture medium

  • @ref: 5370
  • name: ALKALIPHILIC SULPHUR RESPIRING STRAINS MEDIUM (DSMZ Medium 925)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/925
  • composition: Name: ALKALIPHILIC SULPHUR RESPIRING STRAINS MEDIUM (DSMZ Medium 925) Composition: Na2CO3 20.0 g/l NaHCO3 10.0 g/l NaCl 5.0 g/l K2HPO4 1.0 g/l MgCl2 x 6 H2O 0.2 g/l H3BO3 0.0006 g/l CoCl2 x 6 H2O 0.0004 g/l ZnSO4 x 7 H2O 0.0002 g/l Na2MoO4 x 2 H2O 6e-05 g/l MnCl2 x 4 H2O 6e-05 g/l NiCl2 x 6 H2O 4e-05 g/l CuCl2 x 2 H2O 2e-05 g/l EDTA 1e-05 g/l FeSO4 x 7 H2O 4e-06 g/l Distilled water

culture temp

  • @ref: 5370
  • growth: positive
  • type: growth
  • temperature: 28
  • range: mesophilic

culture pH

@refabilitytypepHPH range
43182positiveoptimum9.5alkaliphile
43182positiveminimum7.5
43182positivemaximum10.6

Physiology and metabolism

oxygen tolerance

  • @ref: 43182
  • oxygen tolerance: obligate aerobe

nutrition type

  • @ref: 43182
  • type: chemolithoautotroph

halophily

  • @ref: 43182
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 1.5 M

observation

  • @ref: 43182
  • observation: produce carboxysomes

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4318215226tetrathionate+energy source
4318216094thiosulfate+electron donor
4318233403elemental sulfur+electron donor
4318215226tetrathionate+electron donor
4318215987trithionate+electron donor
4318215138sulfide+electron donor
4318218022thiocyanate-electron donor
4318217359sulfite-electron donor
4318218248iron-electron donor
4318218291manganese-electron donor
4318218276dihydrogen-electron donor

metabolite production

@refChebi-IDmetaboliteproduction
4318230411cobalaminyes
4318233403elemental sulfurno

enzymes

  • @ref: 43182
  • value: cytochrome oxidase
  • activity: +
  • ec: 1.9.3.1

Isolation, sampling and environmental information

isolation

  • @ref: 5370
  • sample type: water
  • geographic location: California, Lake Mono
  • country: USA
  • origin.country: USA
  • continent: North America

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic

Safety information

risk assessment

  • @ref: 5370
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 5370
  • description: Thioalkalimicrobium cyclum 16S ribosomal RNA gene, partial sequence
  • accession: AF329082
  • length: 1500
  • database: ena
  • NCBI tax ID: 717773

GC content

@refGC-contentmethod
537049.6thermal denaturation, midpoint method (Tm)
4318247

External links

@ref: 5370

culture collection no.: DSM 14477, JCM 11371, ALM 1

straininfo link

  • @ref: 81126
  • straininfo: 101019

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny12054257Thioalkalimicrobium cyclicum sp. nov. and Thioalkalivibrio jannaschii sp. nov., novel species of haloalkaliphilic, obligately chemolithoautotrophic sulfur-oxidizing bacteria from hypersaline alkaline Mono Lake (California).Sorokin DY, Gorlenko VM, Tourova TP, Tsapin AI, Nealson KH, Kuenen GJInt J Syst Evol Microbiol10.1099/00207713-52-3-9132002California, Culture Media, DNA, Ribosomal/analysis, Fresh Water/*microbiology, Gammaproteobacteria/*classification/genetics/*physiology/ultrastructure, Hydrogen-Ion Concentration, Molecular Sequence Data, Oxidation-Reduction, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/*pharmacology, Sulfur/*metabolismCultivation
Genetics27274784Complete genome sequence of the haloalkaliphilic, obligately chemolithoautotrophic thiosulfate and sulfide-oxidizing gamma-proteobacterium Thioalkalimicrobium cyclicum type strain ALM 1 (DSM 14477(T)).Kappler U, Davenport K, Beatson S, Lapidus A, Pan C, Han C, Montero-Calasanz Mdel C, Land M, Hauser L, Rohde M, Goker M, Ivanova N, Woyke T, Klenk HP, Kyrpides NCStand Genomic Sci10.1186/s40793-016-0162-x2016

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5370Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14477)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14477
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
43182Rich Boden, Kathleen M. Scott, John Williams, Sydney Russel, Kirsten Antonen, Alex W. Rae and Lee P. Hutt10.1099/ijsem.0.001855An evaluation of Thiomicrospira, Hydrogenovibrio and Thioalkalimicrobium: reclassification of four species of Thiomicrospira to each Thiomicrorhabdus gen. nov. and Hydrogenovibrio, and reclassification of all four species of Thioalkalimicrobium to ThiomicrospiraIJSEM 67: 1140-1151 201728581925
66793Mukherjee et al.10.1038/nbt.3886GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life 35: 676-683 201728604660
81126Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID101019.1StrainInfo: A central database for resolving microbial strain identifiers