Strain identifier
BacDive ID: 11906
Type strain: 
Species: Methylophaga alcalica
Strain Designation: M39
Strain history: <- Y. A. Trotsenko; M39 <- N. V. Doronina
NCBI tax ID(s): 182774 (species)
version 8.1 (current version)
General
@ref: 5616
BacDive-ID: 11906
DSM-Number: 14953
keywords: 16S sequence, Bacteria, mesophilic
description: Methylophaga alcalica M39 is a mesophilic bacterium that was isolated from saline soda lake Khotontyn-nur.
NCBI tax id
- NCBI tax id: 182774
- Matching level: species
strain history
- @ref: 5616
- history: <- Y. A. Trotsenko; M39 <- N. V. Doronina
doi: 10.13145/bacdive11906.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Beggiatoales
- family: Piscirickettsiaceae
- genus: Methylophaga
- species: Methylophaga alcalica
- full scientific name: Methylophaga alcalica Doronina et al. 2003
@ref: 5616
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Thiotrichales
family: Piscirickettsiaceae
genus: Methylophaga
species: Methylophaga alcalica
full scientific name: Methylophaga alcalica Doronina et al. 2003
strain designation: M39
type strain: yes
Culture and growth conditions
culture medium
- @ref: 5616
- name: METHYLOPHAGA ALCALICA MEDIUM (DSMZ Medium 976)
- growth: yes
- link: https://mediadive.dsmz.de/medium/976
- composition: Name: METHYLOPHAGA ALCALICA MEDIUM (DSMZ Medium 976) Composition: NaCl 30.0 g/l Methanol 7.92 g/l Na2CO3 5.0 g/l KNO3 1.0 g/l KH2PO4 1.0 g/l MgSO4 x 7 H2O 0.22 g/l Agar 0.02 g/l CuSO4 x 5 H2O 0.0005 g/l CaCl2 x 2 H2O 3e-05 g/l Ferric citrate 3e-05 g/l ZnSO4 x 7 H2O 5e-06 g/l MgCl2 x 4 H2O 5e-06 g/l Distilled water
culture temp
- @ref: 5616
- growth: positive
- type: growth
- temperature: 30
- range: mesophilic
Isolation, sampling and environmental information
isolation
- @ref: 5616
- sample type: saline soda lake Khotontyn-nur
- country: Mongolia
- origin.country: MNG
- continent: Asia
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Lake (large) |
| #Condition | #Alkaline | |
| #Condition | #Saline |
Safety information
risk assessment
- @ref: 5616
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 20218
- description: Methylophaga alcalica 16S ribosomal RNA gene, partial sequence
- accession: AF384373
- length: 1494
- database: ena
- NCBI tax ID: 182774
GC content
- @ref: 5616
- GC-content: 48.3
- method: thermal denaturation, midpoint method (Tm)
External links
@ref: 5616
culture collection no.: DSM 14953, ATCC BAA 297, VKM B-2251
straininfo link
- @ref: 81121
- straininfo: 100768
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 12656177 | Methylophaga alcalica sp. nov., a novel alkaliphilic and moderately halophilic, obligately methylotrophic bacterium from an East Mongolian saline soda lake. | Doronina NV, Darmaeva TD, Trotsenko YA | Int J Syst Evol Microbiol | 10.1099/ijs.0.02267-0 | 2003 | Base Composition, Base Sequence, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Gammaproteobacteria/*classification/genetics/*isolation & purification/metabolism, Genotype, Microscopy, Electron, Molecular Sequence Data, Mongolia, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Species Specificity, Water Microbiology | Genetics |
| Phylogeny | 21890731 | Methylophaga lonarensis sp. nov., a moderately haloalkaliphilic methylotroph isolated from the soda lake sediments of a meteorite impact crater. | Antony CP, Doronina NV, Boden R, Trotsenko YA, Shouche YS, Murrell JC | Int J Syst Evol Microbiol | 10.1099/ijs.0.035089-0 | 2011 | Base Composition, Carbon/metabolism, Carbonates/metabolism, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Geologic Sediments/*microbiology, Hydrogen-Ion Concentration, Lakes, Locomotion, Metabolic Networks and Pathways, Meteoroids, Methane/metabolism, Molecular Sequence Data, Nucleic Acid Hybridization, Pentoses/metabolism, Phospholipids/analysis, Phylogeny, Piscirickettsiaceae/*classification/genetics/*isolation & purification/physiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/metabolism, Temperature, Ubiquinone/analysis, Vitamin B 12/metabolism | Metabolism |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 5616 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14953) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-14953 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 81121 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID100768.1 | StrainInfo: A central database for resolving microbial strain identifiers |