Strain identifier
BacDive ID: 11897
Type strain:
Species: Chelativorans multitrophicus
Strain history: <- T. Egli, EAWAG, Swiss Fed. Inst. Aquatic Sci. & Technol., Dubendorf, Switzerland
NCBI tax ID(s): 449973 (species)
General
@ref: 3464
BacDive-ID: 11897
DSM-Number: 9103
keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, rod-shaped
description: Chelativorans multitrophicus DSM 9103 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from mixture of soil extracts and activated sludge from industrial wastewater treatment plants.
NCBI tax id
- NCBI tax id: 449973
- Matching level: species
strain history
- @ref: 3464
- history: <- T. Egli, EAWAG, Swiss Fed. Inst. Aquatic Sci. & Technol., Dubendorf, Switzerland
doi: 10.13145/bacdive11897.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Hyphomicrobiales
- family: Phyllobacteriaceae
- genus: Chelativorans
- species: Chelativorans multitrophicus
- full scientific name: Chelativorans multitrophicus Doronina et al. 2010
synonyms
- @ref: 20215
- synonym: Chelativorans oligotrophicus
@ref: 3464
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Rhizobiales
family: Phyllobacteriaceae
genus: Chelativorans
species: Chelativorans multitrophicus
full scientific name: Chelativorans multitrophicus Doronina et al. 2010
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility | confidence |
---|---|---|---|---|---|---|
28982 | negative | 1.35 µm | 0.75 µm | rod-shaped | no | |
69480 | negative | 99.984 |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
3464 | R2A MEDIUM (DSMZ Medium 830) | yes | https://mediadive.dsmz.de/medium/830 | Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water |
3464 | PLATE COUNT AGAR (DSMZ Medium 464) | yes | https://mediadive.dsmz.de/medium/464 | Name: PLATE COUNT AGAR (DSMZ Medium 464) Composition: Agar 15.0 g/l Tryptone 5.0 g/l Yeast extract 2.5 g/l Dextrose 1.0 g/l Distilled water |
3464 | EDTA-MEDIUM (DSMZ Medium 463a) | yes | https://mediadive.dsmz.de/medium/463a | Name: EDTA-MEDIUM (DSMZ Medium 463a) Composition: MgSO4 x 7 H2O 1.0 g/l EDTA 0.5 g/l Na2HPO4 x 2 H2O 0.41 g/l CaCl2 x 2 H2O 0.4 g/l KH2PO4 0.26 g/l FeCl2 x 4 H2O 0.0015 g/l CoCl2 x 6 H2O 0.00012 g/l MnCl2 x 4 H2O 0.0001 g/l ZnCl2 6.8e-05 g/l H3BO3 6.2e-05 g/l Thiamine-HCl x 2 H2O 5e-05 g/l p-Aminobenzoic acid 5e-05 g/l alpha-lipoic acid 5e-05 g/l Vitamin B12 5e-05 g/l Pantothenic acid 5e-05 g/l Riboflavin 5e-05 g/l Nicotine amide 2.5e-05 g/l Nicotinic acid 2.5e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l Na2MoO4 x 2 H2O 2.4e-05 g/l Folic acid 2e-05 g/l Biotin 2e-05 g/l CuCl2 x 2 H2O 1.7e-05 g/l Pyridoxamine hydrochloride 1e-05 g/l HCl Distilled water |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
3464 | positive | growth | 28 | mesophilic |
28982 | positive | growth | 25-35 | mesophilic |
28982 | positive | optimum | 30 | mesophilic |
culture pH
- @ref: 28982
- ability: positive
- type: optimum
- pH: 7
Physiology and metabolism
oxygen tolerance
- @ref: 28982
- oxygen tolerance: aerobe
spore formation
@ref | spore formation | confidence |
---|---|---|
28982 | no | |
69481 | no | 100 |
69480 | no | 100 |
halophily
- @ref: 28982
- salt: NaCl
- growth: positive
- tested relation: growth
- concentration: <3 %
metabolite production
- @ref: 28982
- Chebi-ID: 35581
- metabolite: indole
- production: yes
metabolite tests
- @ref: 28982
- Chebi-ID: 35581
- metabolite: indole
- indole test: +
enzymes
@ref | value | activity | ec |
---|---|---|---|
28982 | catalase | + | 1.11.1.6 |
28982 | cytochrome oxidase | + | 1.9.3.1 |
Isolation, sampling and environmental information
isolation
- @ref: 3464
- sample type: mixture of soil extracts and activated sludge from industrial wastewater treatment plants
- country: Switzerland
- origin.country: CHE
- continent: Europe
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Waste | #Activated sludge |
#Engineered | #Waste | #Industrial wastewater |
#Engineered | #Waste | #Water treatment plant |
#Environmental | #Terrestrial | #Soil |
taxonmaps
- @ref: 69479
- File name: preview.99_6742.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_5;96_220;97_3238;98_4971;99_6742&stattab=map
- Last taxonomy: Mesorhizobium
- 16S sequence: EF457243
- Sequence Identity:
- Total samples: 1910
- soil counts: 603
- aquatic counts: 392
- animal counts: 759
- plant counts: 156
Safety information
risk assessment
- @ref: 3464
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 3464
- description: Chelativorans multitrophicus strain DSM 9103 16S ribosomal RNA gene, partial sequence
- accession: EF457243
- length: 1415
- database: ena
- NCBI tax ID: 449973
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Chelativorans multitrophicus DSM 9103 | GCA_011317445 | contig | ncbi | 449973 |
66792 | Chelativorans multitrophicus strain DSM 9103 | 449973.3 | wgs | patric | 449973 |
GC content
@ref | GC-content | method |
---|---|---|
3464 | 63.1 | thermal denaturation, midpoint method (Tm) |
28982 | 63.1 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 100 | no |
gram-positive | no | 98.425 | no |
anaerobic | no | 97.631 | no |
halophile | no | 80.507 | no |
spore-forming | no | 94.963 | yes |
glucose-util | yes | 90.434 | no |
aerobic | yes | 88.51 | yes |
thermophile | no | 92.468 | no |
motile | yes | 67.672 | yes |
flagellated | no | 72.263 | no |
glucose-ferment | no | 90.134 | no |
External links
@ref: 3464
culture collection no.: DSM 9103, VKM B-2394
straininfo link
- @ref: 81114
- straininfo: 215465
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 19666787 | Chelativorans multitrophicus gen. nov., sp. nov. and Chelativorans oligotrophicus sp. nov., aerobic EDTA-degrading bacteria. | Doronina NV, Kaparullina EN, Trotsenko YA, Nortemann B, Bucheli-Witschel M, Weilenmann HU, Egli T | Int J Syst Evol Microbiol | 10.1099/ijs.0.003152-0 | 2009 | Aerobiosis, Alphaproteobacteria/*classification/genetics/metabolism/physiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Edetic Acid/*metabolism, Fatty Acids/analysis, Genes, rRNA, Genotype, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Russia, Sequence Analysis, DNA, Sewage/*microbiology, Species Specificity, Switzerland | Genetics |
Phylogeny | 33037888 | Chelativorans xinjiangense sp. nov., a novel bacterial species isolated from soil in Xinjiang, China. | Meng D, Liu YL, Gu PF, Fan XY, Huang ZS, Ji Y, Li WM, Du ZJ, Li Q | Arch Microbiol | 10.1007/s00203-020-02064-x | 2020 | Base Composition, China, Fabaceae/microbiology, Fatty Acids/analysis, Phospholipids/chemistry, Phyllobacteriaceae/chemistry/*classification/genetics, Phylogeny, RNA, Ribosomal, 16S/genetics, Rhizosphere, *Soil Microbiology, Species Specificity | |
Phylogeny | 33651188 | Chelativorans alearense sp. nov., A Novel Bacterial Species Isolated From Soil in Alear, China. | Meng D, Liu YL, Gu PF, Fan XY, Huang ZS, Ji Y, Li WM, Du ZJ, Li Q | Curr Microbiol | 10.1007/s00284-021-02428-6 | 2021 | Bacterial Typing Techniques, China, DNA, Bacterial/genetics, Fatty Acids/analysis, *Phospholipids, Phyllobacteriaceae, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil | Transcriptome |
Reference
@id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
---|---|---|---|---|---|---|
3464 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 9103) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-9103 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
28982 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 25415 | 28776041 | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
81114 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID215465.1 | StrainInfo: A central database for resolving microbial strain identifiers |