Strain identifier

BacDive ID: 11897

Type strain: Yes

Species: Chelativorans multitrophicus

Strain history: <- T. Egli, EAWAG, Swiss Fed. Inst. Aquatic Sci. & Technol., Dubendorf, Switzerland

NCBI tax ID(s): 449973 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 3464

BacDive-ID: 11897

DSM-Number: 9103

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, rod-shaped

description: Chelativorans multitrophicus DSM 9103 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from mixture of soil extracts and activated sludge from industrial wastewater treatment plants.

NCBI tax id

  • NCBI tax id: 449973
  • Matching level: species

strain history

  • @ref: 3464
  • history: <- T. Egli, EAWAG, Swiss Fed. Inst. Aquatic Sci. & Technol., Dubendorf, Switzerland

doi: 10.13145/bacdive11897.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Phyllobacteriaceae
  • genus: Chelativorans
  • species: Chelativorans multitrophicus
  • full scientific name: Chelativorans multitrophicus Doronina et al. 2010
  • synonyms

    • @ref: 20215
    • synonym: Chelativorans oligotrophicus

@ref: 3464

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Phyllobacteriaceae

genus: Chelativorans

species: Chelativorans multitrophicus

full scientific name: Chelativorans multitrophicus Doronina et al. 2010

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
28982negative1.35 µm0.75 µmrod-shapedno
69480negative99.984

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3464R2A MEDIUM (DSMZ Medium 830)yeshttps://mediadive.dsmz.de/medium/830Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
3464PLATE COUNT AGAR (DSMZ Medium 464)yeshttps://mediadive.dsmz.de/medium/464Name: PLATE COUNT AGAR (DSMZ Medium 464) Composition: Agar 15.0 g/l Tryptone 5.0 g/l Yeast extract 2.5 g/l Dextrose 1.0 g/l Distilled water
3464EDTA-MEDIUM (DSMZ Medium 463a)yeshttps://mediadive.dsmz.de/medium/463aName: EDTA-MEDIUM (DSMZ Medium 463a) Composition: MgSO4 x 7 H2O 1.0 g/l EDTA 0.5 g/l Na2HPO4 x 2 H2O 0.41 g/l CaCl2 x 2 H2O 0.4 g/l KH2PO4 0.26 g/l FeCl2 x 4 H2O 0.0015 g/l CoCl2 x 6 H2O 0.00012 g/l MnCl2 x 4 H2O 0.0001 g/l ZnCl2 6.8e-05 g/l H3BO3 6.2e-05 g/l Thiamine-HCl x 2 H2O 5e-05 g/l p-Aminobenzoic acid 5e-05 g/l alpha-lipoic acid 5e-05 g/l Vitamin B12 5e-05 g/l Pantothenic acid 5e-05 g/l Riboflavin 5e-05 g/l Nicotine amide 2.5e-05 g/l Nicotinic acid 2.5e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l Na2MoO4 x 2 H2O 2.4e-05 g/l Folic acid 2e-05 g/l Biotin 2e-05 g/l CuCl2 x 2 H2O 1.7e-05 g/l Pyridoxamine hydrochloride 1e-05 g/l HCl Distilled water

culture temp

@refgrowthtypetemperaturerange
3464positivegrowth28mesophilic
28982positivegrowth25-35mesophilic
28982positiveoptimum30mesophilic

culture pH

  • @ref: 28982
  • ability: positive
  • type: optimum
  • pH: 7

Physiology and metabolism

oxygen tolerance

  • @ref: 28982
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
28982no
69481no100
69480no100

halophily

  • @ref: 28982
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: <3 %

metabolite production

  • @ref: 28982
  • Chebi-ID: 35581
  • metabolite: indole
  • production: yes

metabolite tests

  • @ref: 28982
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: +

enzymes

@refvalueactivityec
28982catalase+1.11.1.6
28982cytochrome oxidase+1.9.3.1

Isolation, sampling and environmental information

isolation

  • @ref: 3464
  • sample type: mixture of soil extracts and activated sludge from industrial wastewater treatment plants
  • country: Switzerland
  • origin.country: CHE
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Engineered#Waste#Activated sludge
#Engineered#Waste#Industrial wastewater
#Engineered#Waste#Water treatment plant
#Environmental#Terrestrial#Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_6742.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_5;96_220;97_3238;98_4971;99_6742&stattab=map
  • Last taxonomy: Mesorhizobium
  • 16S sequence: EF457243
  • Sequence Identity:
  • Total samples: 1910
  • soil counts: 603
  • aquatic counts: 392
  • animal counts: 759
  • plant counts: 156

Safety information

risk assessment

  • @ref: 3464
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 3464
  • description: Chelativorans multitrophicus strain DSM 9103 16S ribosomal RNA gene, partial sequence
  • accession: EF457243
  • length: 1415
  • database: ena
  • NCBI tax ID: 449973

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Chelativorans multitrophicus DSM 9103GCA_011317445contigncbi449973
66792Chelativorans multitrophicus strain DSM 9103449973.3wgspatric449973

GC content

@refGC-contentmethod
346463.1thermal denaturation, midpoint method (Tm)
2898263.1

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
gram-positiveno98.425no
anaerobicno97.631no
halophileno80.507no
spore-formingno94.963yes
glucose-utilyes90.434no
aerobicyes88.51yes
thermophileno92.468no
motileyes67.672yes
flagellatedno72.263no
glucose-fermentno90.134no

External links

@ref: 3464

culture collection no.: DSM 9103, VKM B-2394

straininfo link

  • @ref: 81114
  • straininfo: 215465

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19666787Chelativorans multitrophicus gen. nov., sp. nov. and Chelativorans oligotrophicus sp. nov., aerobic EDTA-degrading bacteria.Doronina NV, Kaparullina EN, Trotsenko YA, Nortemann B, Bucheli-Witschel M, Weilenmann HU, Egli TInt J Syst Evol Microbiol10.1099/ijs.0.003152-02009Aerobiosis, Alphaproteobacteria/*classification/genetics/metabolism/physiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Edetic Acid/*metabolism, Fatty Acids/analysis, Genes, rRNA, Genotype, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Russia, Sequence Analysis, DNA, Sewage/*microbiology, Species Specificity, SwitzerlandGenetics
Phylogeny33037888Chelativorans xinjiangense sp. nov., a novel bacterial species isolated from soil in Xinjiang, China.Meng D, Liu YL, Gu PF, Fan XY, Huang ZS, Ji Y, Li WM, Du ZJ, Li QArch Microbiol10.1007/s00203-020-02064-x2020Base Composition, China, Fabaceae/microbiology, Fatty Acids/analysis, Phospholipids/chemistry, Phyllobacteriaceae/chemistry/*classification/genetics, Phylogeny, RNA, Ribosomal, 16S/genetics, Rhizosphere, *Soil Microbiology, Species Specificity
Phylogeny33651188Chelativorans alearense sp. nov., A Novel Bacterial Species Isolated From Soil in Alear, China.Meng D, Liu YL, Gu PF, Fan XY, Huang ZS, Ji Y, Li WM, Du ZJ, Li QCurr Microbiol10.1007/s00284-021-02428-62021Bacterial Typing Techniques, China, DNA, Bacterial/genetics, Fatty Acids/analysis, *Phospholipids, Phyllobacteriaceae, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *SoilTranscriptome

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
3464Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 9103)https://www.dsmz.de/collection/catalogue/details/culture/DSM-9103
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
28982Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2541528776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
81114Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID215465.1StrainInfo: A central database for resolving microbial strain identifiers